BR0015382A - Método para sintetização do ácido nucléico - Google Patents

Método para sintetização do ácido nucléico

Info

Publication number
BR0015382A
BR0015382A BR0015382-6A BR0015382A BR0015382A BR 0015382 A BR0015382 A BR 0015382A BR 0015382 A BR0015382 A BR 0015382A BR 0015382 A BR0015382 A BR 0015382A
Authority
BR
Brazil
Prior art keywords
nucleic acid
primer
present
synthesis method
oligonucleotide
Prior art date
Application number
BR0015382-6A
Other languages
English (en)
Other versions
BR0015382B1 (pt
Inventor
Tsugunori Notomi
Tetsu Hase
Original Assignee
Eiken Chemical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=14237231&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0015382(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/JP1999/006213 external-priority patent/WO2000028082A1/ja
Application filed by Eiken Chemical filed Critical Eiken Chemical
Publication of BR0015382A publication Critical patent/BR0015382A/pt
Publication of BR0015382B1 publication Critical patent/BR0015382B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Patente de Invenção: "MéTODO PARA SINTETIZAçãO DO áCIDO NUCLéICO". A presente invenção refere-se a um oligonucleotídeo tendo uma nova estrutura e um método de sintetização de ácido nucléico empregando-se o mesmo como um iniciador. Este oligonucleotídeo é fornecido no lado 5' do iniciador com uma seq³ência de nucleotídeo substancialmente a mesma como a região sintetizada com este iniciador como a origem de síntese. A presente invenção realiza a síntese de ácido nucléico com base em uma reação isotérmica com uma constituição simples de reagentes. Além disso, a presente invenção fornece um método de sintetização de ácido nucléico altamente específico nas bases deste método de sintetização de ácido nucléico.
BRPI0015382-6A 1999-11-08 2000-03-28 Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia BR0015382B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/JP1999/006213 WO2000028082A1 (fr) 1998-11-09 1999-11-08 Procede de synthese d'acide nucleique
JPPCT/JP99/06213 1999-11-08
PCT/JP2000/001919 WO2001034790A1 (fr) 1999-11-08 2000-03-28 Procede de synthese d'un acide nucleique

Publications (2)

Publication Number Publication Date
BR0015382A true BR0015382A (pt) 2002-07-02
BR0015382B1 BR0015382B1 (pt) 2014-04-29

Family

ID=14237231

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0015382-6A BR0015382B1 (pt) 1999-11-08 2000-03-28 Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia

Country Status (9)

Country Link
KR (1) KR100612551B1 (pt)
CN (2) CN100393875C (pt)
BR (1) BR0015382B1 (pt)
CA (1) CA2390309C (pt)
IL (1) IL149446A0 (pt)
NO (1) NO331732B1 (pt)
RU (1) RU2252964C2 (pt)
WO (1) WO2001034790A1 (pt)
ZA (1) ZA200203293B (pt)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
EP1275715B1 (en) * 2000-04-07 2015-07-15 Eiken Kagaku Kabushiki Kaisha Method for amplifyingand detecting a nucleic acid by using double-stranded nucleic acid as template
ATE437225T1 (de) 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
KR100806680B1 (ko) * 2003-11-21 2008-02-26 한국표준과학연구원 Ｐｃｒ용 열순환 반응기의 성능시험용 다중 ｐｃｒ 조성물
DK2412718T3 (en) * 2009-03-26 2017-01-30 Xiamen Amoy Diagnostics Co Ltd CIRCUIT PRIMES USED BY NUCLEIC ACID AMPLIFICATION AND USE THEREOF
CN101671674B (zh) * 2009-03-27 2010-09-22 郑立谋 一种用于核酸扩增的环形引物及其应用
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
TWI600766B (zh) * 2012-08-09 2017-10-01 財團法人工業技術研究院 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組
ES2887177T3 (es) 2013-03-13 2021-12-22 Illumina Inc Método de preparación de una biblioteca de secuenciación de ácidos nucleicos
WO2014145298A1 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nucleic acid amplification
EP2971123B1 (en) 2013-03-15 2021-05-05 Labrador Diagnostics LLC Nucleic acid amplification
WO2015075198A1 (en) * 2013-11-22 2015-05-28 Orion Diagnostica Oy Detection of nucleic acids by strand invasion based amplification
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
BR112017007912A2 (pt) * 2014-10-17 2018-01-23 Illumina Cambridge Limited transposon de preservação de contiguidade
CN107208012B (zh) 2014-11-03 2021-06-29 唐恩生物科学公司 用于细胞、孢子或病毒捕获和破碎的装置和方法
ES2764975T3 (es) 2014-12-15 2020-06-05 Cepheid Amplificación de ácidos nucleicos en base exponencial mayor de 2
WO2018132939A1 (zh) * 2017-01-17 2018-07-26 中国科学院过程工程研究所 一种恒温条件下合成核酸的方法
EP3476938B1 (en) * 2017-09-14 2020-05-13 Zhongke Xinray (Suzhou) Biological Science Technologies Co., Ltd. Method and kit for synthesizing nucleic acid under constant temperature conditions
CN113227398A (zh) 2018-10-29 2021-08-06 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
CN113302314A (zh) 2019-01-15 2021-08-24 3M创新有限公司 用于产志贺毒素大肠杆菌(stec)检测的环介导的等温扩增引物
CN115103919A (zh) 2020-02-17 2022-09-23 3M创新有限公司 用于副溶血弧菌检测的环介导的等温扩增引物及其用途
CA3186580A1 (en) 2020-06-12 2021-12-16 Sherlock Biosciences, Inc. Crispr-based sars-cov-2 detection
WO2022029449A1 (en) 2020-08-07 2022-02-10 Oxford Nanopore Technologies Limited Methods of identifying nucleic acid barcodes
CN113201583B (zh) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
WO2022226870A1 (zh) * 2021-04-29 2022-11-03 中国科学院大学宁波生命与健康产业研究院 恒温条件下合成核酸的方法及试剂盒和应用
KR20240072313A (ko) 2022-11-10 2024-05-24 (주)레보스케치 황색포도상구균 감염 진단을 위한 rt-lamp용 조성물 및 이의 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
ES2112302T3 (es) * 1991-10-11 1998-04-01 Behringwerke Ag Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos.
WO1993017127A1 (en) * 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
FR2721945B1 (fr) * 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
WO2001034838A1 (fr) * 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Methode de detection d'une variation ou d'un polymorphisme

Also Published As

Publication number Publication date
WO2001034790A1 (fr) 2001-05-17
RU2252964C2 (ru) 2005-05-27
KR100612551B1 (ko) 2006-08-11
IL149446A0 (en) 2002-11-10
NO20022171D0 (no) 2002-05-07
KR20020064896A (ko) 2002-08-10
CA2390309C (en) 2012-09-25
CN1420928A (zh) 2003-05-28
CN100393875C (zh) 2008-06-11
RU2002115268A (ru) 2004-01-27
ZA200203293B (en) 2003-03-26
CN1876843B (zh) 2012-09-05
NO331732B1 (no) 2012-03-12
BR0015382B1 (pt) 2014-04-29
NO20022171L (no) 2002-07-04
CA2390309A1 (en) 2001-05-17
CN1876843A (zh) 2006-12-13

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Legal Events

Date Code Title Description
B25G Requested change of headquarter approved

Owner name: EIKEN KAGAKU KABUSHIKI KAISHA (JP)

Free format text: SEDE ALTERADA CONFORME SOLICITADO NA PETICAO NO 020080153598/RJ DE 12/12/2008.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULACAO DA PUBLICACAO POR TER SIDO INDEVIDA REF, A RPI 2228 DE 17/09/2013

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 29/04/2014, OBSERVADAS AS CONDICOES LEGAIS.