ATE280177T1 - Modifizierte primer - Google Patents

Modifizierte primer

Info

Publication number
ATE280177T1
ATE280177T1 AT98104461T AT98104461T ATE280177T1 AT E280177 T1 ATE280177 T1 AT E280177T1 AT 98104461 T AT98104461 T AT 98104461T AT 98104461 T AT98104461 T AT 98104461T AT E280177 T1 ATE280177 T1 AT E280177T1
Authority
AT
Austria
Prior art keywords
carried out
amplification product
modified primers
modified oligonucleotides
primers
Prior art date
Application number
AT98104461T
Other languages
English (en)
Inventor
Stephen Gordon Will
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE280177T1 publication Critical patent/ATE280177T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
AT98104461T 1997-03-20 1998-03-12 Modifizierte primer ATE280177T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4112797P 1997-03-20 1997-03-20

Publications (1)

Publication Number Publication Date
ATE280177T1 true ATE280177T1 (de) 2004-11-15

Family

ID=21914898

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98104461T ATE280177T1 (de) 1997-03-20 1998-03-12 Modifizierte primer

Country Status (21)

Country Link
US (1) US6001611A (de)
EP (1) EP0866071B1 (de)
JP (1) JP2966389B2 (de)
KR (1) KR100292997B1 (de)
CN (1) CN1065873C (de)
AT (1) ATE280177T1 (de)
AU (1) AU712448B2 (de)
BR (1) BR9801878B1 (de)
CA (1) CA2229766C (de)
CZ (1) CZ291525B6 (de)
DE (1) DE69827060T2 (de)
DK (1) DK0866071T3 (de)
ES (1) ES2230631T3 (de)
HK (1) HK1012192A1 (de)
HU (1) HU223669B1 (de)
IL (1) IL123694A (de)
NO (1) NO322136B1 (de)
PL (1) PL190142B1 (de)
RU (1) RU2159248C2 (de)
TW (1) TW567188B (de)
UA (1) UA49843C2 (de)

Families Citing this family (180)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
WO2001037291A1 (en) 1999-11-17 2001-05-25 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
ES2253279T3 (es) 1999-12-15 2006-06-01 Gen-Probe Incorporated Metodos y composiciones para la deteccion de especies del complejo de mycobacterium avium.
WO2001044510A2 (en) * 1999-12-17 2001-06-21 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US6664081B2 (en) 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
WO2001075139A1 (en) * 2000-04-03 2001-10-11 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
US6548251B1 (en) * 2000-09-05 2003-04-15 Fidelity Systems, Inc. Inhibition of molecular and biological processes using modified oligonucleotides
US20020031777A1 (en) * 2000-09-08 2002-03-14 Linda Starr-Spires Ultra yield amplification reaction
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
ES2254306T3 (es) * 2000-10-25 2006-06-16 F. Hoffmann-La Roche Ag Amplificacion usando cebadores modificados.
WO2002083839A2 (en) * 2001-03-30 2002-10-24 Amersham Biosciences Ab P450 single nucleotide polymorphism biochip analysis
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
WO2002101041A1 (fr) * 2001-06-12 2002-12-19 Takara Bio Inc. Procede d'amplification de l'acide nucleique et procede de detection du polymorphisme des nucleotides a l'aide d'un analogue de nucleotide
WO2003020983A1 (en) * 2001-08-30 2003-03-13 Virginia Commonwealth University Allele specific pcr for genotyping
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー/アクセプター部分が相補鎖上となる標的核酸検出のmet/fretに基づく方法
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7408051B2 (en) 2004-04-14 2008-08-05 Applera Corporation Modified oligonucleotides and applications thereof
US7517978B1 (en) 2004-04-14 2009-04-14 Applied Biosystems, Llc Modified oligonucleotides and applications thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2006112818A2 (en) * 2005-04-14 2006-10-26 Applera Corporation 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes
WO2006006722A1 (ja) 2004-07-13 2006-01-19 Takeda Pharmaceutical Company Limited 細胞機能の調節方法
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20090118132A1 (en) * 2004-11-04 2009-05-07 Roche Molecular Systems, Inc. Classification of Acute Myeloid Leukemia
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
ATE502122T1 (de) * 2006-06-01 2011-04-15 Trilink Biotechnologies Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
WO2007147110A2 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
ATE538216T1 (de) * 2006-07-31 2012-01-15 Wanli Bi Nukleinsäureverstärkung mithilfe eines reversibel modifizierten oligonukleotids
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (en) 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7893227B2 (en) 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
DK2574681T3 (en) 2007-03-28 2016-07-04 Signal Diagnostics System and method for high-resolution analysis of nucleic acids to detect sequence variations
CN103320506B (zh) 2007-07-03 2015-10-28 吉纳珀莱有限公司 用于改进的核酸扩增反应的嵌合引物
ES2453108T3 (es) 2007-08-06 2014-04-04 Orion Genomics, Llc Nuevos polimorfismos de un único nucleótido y combinaciones de polimorfismos nuevos y conocidos para determinar la expresión específica de alelo del gen IGF2
JP2009077712A (ja) 2007-09-11 2009-04-16 F Hoffmann La Roche Ag B−Rafキナーゼ阻害剤に対する感受性についての診断試験
EP2207896B1 (de) * 2007-10-04 2014-01-29 Commonwealth Scientific and Industrial Research Organisation Nukleinsäureamplifikation
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A2 (de) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
EP2644709B1 (de) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CN103588839B (zh) 2008-06-11 2017-04-12 激光基因公司 核苷酸和核苷以及将其用于dna测序的方法
CA2731991C (en) 2008-08-04 2021-06-08 Gene Security Network, Inc. Methods for allele calling and ploidy calling
CN102186996A (zh) * 2008-10-20 2011-09-14 霍夫曼-拉罗奇有限公司 改进的等位基因特异性扩增
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
AU2010213727A1 (en) 2009-02-11 2011-09-29 Orion Genomics Llc Combinations of polymorphisms for determining allele-specific expression of IGF2
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
EP2473638B1 (de) 2009-09-30 2017-08-09 Natera, Inc. Verfahren zur nichtinvasiven pränatalen ploidiezuordnung
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
WO2011146632A1 (en) 2010-05-18 2011-11-24 Gene Security Network Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
ES2647612T3 (es) 2010-06-04 2017-12-22 Chronix Biomedical Biomarcadores de ácidos nucleicos en circulación asociados al cáncer de próstata
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
US20120142908A1 (en) 2010-12-01 2012-06-07 Berry And Associates, Inc. Compounds for the synthetic introduction of n-alkyl nucleosides into dna oligonucleotides
JP6328934B2 (ja) 2010-12-22 2018-05-23 ナテラ, インコーポレイテッド 非侵襲性出生前親子鑑定法
US20120164641A1 (en) * 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
AU2011358564B9 (en) 2011-02-09 2017-07-13 Natera, Inc Methods for non-invasive prenatal ploidy calling
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US9034581B2 (en) * 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
PL2768985T3 (pl) 2011-10-21 2019-10-31 Chronix Biomedical Biomarkery będące krążącymi kwasami nukleinowymi związane z rakiem jelita grubego
ES2413566B1 (es) 2011-12-14 2014-05-20 Fundación Pública Andaluza Progreso Y Salud Método de obtención de datos útiles para el diagnóstico y pronóstico de la hipoacusia neurosensorial.
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
KR101545848B1 (ko) * 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
WO2013188839A1 (en) 2012-06-14 2013-12-19 Life Technologies Corporation Novel compositions, methods and kits for real time polymerase chain reaction (pcr)
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
EP2722399A1 (de) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation
US9382581B2 (en) 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
EP2931922B1 (de) 2012-12-14 2018-10-17 Chronix Biomedical Personalisierte biomarker für krebs
US9828629B2 (en) 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
SI3004388T2 (sl) 2013-05-29 2023-11-30 Chronix Biomedical Odkrivanje in kvantifikacija donorjeve brezcelične DNK v cirkulaciji prejemnikov presajenih organov
US10093978B2 (en) 2013-08-12 2018-10-09 Genentech, Inc. Compositions for detecting complement factor H (CFH) and complement factor I (CFI) polymorphisms
WO2015048535A1 (en) 2013-09-27 2015-04-02 Natera, Inc. Prenatal diagnostic resting standards
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
ES2705573T3 (es) 2013-10-09 2019-03-26 Hoffmann La Roche Procedimientos y composiciones para detectar mutaciones en el gen EZH2 humano
AU2014331828B2 (en) 2013-10-09 2020-05-14 Fluoresentric, Inc. Multiplex probes
MX367192B (es) 2013-10-30 2019-08-08 Green Life Biotech Llc Sistemas de cuantificacion de patogenesis y metodos de tratamiento para el enverdecimiento de los citricos.
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
TW202239429A (zh) 2014-02-08 2022-10-16 美商建南德克公司 治療阿茲海默症之方法
CN113774132A (zh) 2014-04-21 2021-12-10 纳特拉公司 检测染色体片段中的突变和倍性
PL3201361T3 (pl) 2014-10-01 2020-09-07 Chronix Biomedical Sposoby ilościowego oznaczania pozakomórkowego DNA
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (de) 2014-12-18 2018-08-29 Agilome, Inc. Chemisch empfindlicher feldeffekttransistor
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
US9970062B2 (en) * 2015-08-06 2018-05-15 Roche Molecular Systems, Inc. Compositions and methods for detection of mycobacterium tuberculosis
US9382582B1 (en) * 2015-08-25 2016-07-05 Tracy Hayden Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
WO2017153566A1 (en) 2016-03-11 2017-09-14 Roche Diagnostics Gmbh Compositions and methods for detection of zika virus
WO2017169119A1 (ja) * 2016-03-30 2017-10-05 大研医器株式会社 変異プライマーの設計方法
JP6681804B2 (ja) * 2016-03-30 2020-04-15 大研医器株式会社 変異プライマーの設計方法
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
US10934597B2 (en) * 2016-05-27 2021-03-02 Roche Molecular Systems, Inc. Compositions and methods for detection of trichomonas vaginalis
US10612101B2 (en) 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
EP3464617B1 (de) * 2016-05-27 2021-04-07 Roche Diagnostics GmbH Zusammensetzungen und verfahren zur detektion von trichomonas vaginalis
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
US10443095B2 (en) 2016-08-02 2019-10-15 Roche Molecular Systems, Inc. Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
WO2018089942A1 (en) 2016-11-10 2018-05-17 Slipchip Corporation Polynucleotides for the amplification and detection of chlamydia trachomatis
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
CA3049139A1 (en) 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
EP3645710A1 (de) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophile dna-polymerase-mutanten
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
WO2019045532A2 (en) * 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
US20210032609A1 (en) 2018-03-21 2021-02-04 Roche Molecular Systems, Inc. Dna polymerases for efficient and effective incorporation of methylated-dntps
US10450616B1 (en) 2018-05-09 2019-10-22 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Chlamydia trachomatis
US11332787B2 (en) 2018-06-29 2022-05-17 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3874064A1 (de) * 2018-10-29 2021-09-08 Cepheid Exponentielle base-3-nukleinsäureamplifikation mit reduzierter amplifikationszeit unter verwendung verschachtelter überlappender primer
US20220017973A1 (en) 2018-12-03 2022-01-20 Roche Molecular Systems, Inc. Compositions and methods for detection of candida auris
KR102141312B1 (ko) * 2019-04-19 2020-08-04 주식회사 제노헬릭스 짧은 RNA-primed 제노 센서 모듈 증폭 기반 짧은 RNA 탐지 기법
WO2020221915A1 (en) 2019-05-02 2020-11-05 F. Hoffmann-La Roche Ag UTILIZATION OF dITP FOR PREFERENTIAL/SELECTIVE AMPLIFICATION OF RNA VERSUS DNA TARGETS BASED ON STRAND-SEPARATION TEMPERATURE
US20220220539A1 (en) 2019-05-07 2022-07-14 Roche Molecular Systems, Inc. Compositions and methods for detection of neisseria gonorroheae
WO2021013972A1 (en) 2019-07-25 2021-01-28 F. Hoffmann-La Roche Ag Compositions and methods for detection of epstein barr virus (ebv)
US10954572B2 (en) 2019-07-25 2021-03-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Neisseria gonorrhoeae
CN114286866A (zh) 2019-08-27 2022-04-05 豪夫迈·罗氏有限公司 用于扩增和检测包括从cccDNA转录的HBV RNA在内的乙型肝炎病毒RNA的组合物和方法
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
JP2023508100A (ja) 2019-12-27 2023-02-28 エフ. ホフマン-ラ ロシュ エージー. メチシリン耐性黄色ブドウ球菌を検出するための組成物および方法
EP4085156B1 (de) 2019-12-31 2023-10-25 F. Hoffmann-La Roche AG Quantitatives pcr-screening von induzierbaren prophagen aus bakterienisolaten
WO2021180631A1 (en) 2020-03-09 2021-09-16 F. Hoffmann-La Roche Ag Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2), influenza a and influenza b
JP2023536962A (ja) 2020-08-06 2023-08-30 エフ. ホフマン-ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス2(sars-2)、インフルエンザa及びインフルエンザbの検出のための組成物及び方法
EP4256089A1 (de) 2020-12-04 2023-10-11 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis von malaria
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
WO2022167570A1 (en) 2021-02-05 2022-08-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of human parainfluenza viruses 1-4 (hpiv 1-4)
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
EP4334472A1 (de) 2021-05-06 2024-03-13 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis des hepatitis-delta-virus durch einen doppeltarget-test
WO2023079032A1 (en) 2021-11-05 2023-05-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
WO2023089186A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance
WO2023104812A1 (en) 2021-12-09 2023-06-15 F. Hoffmann-La Roche Ag Mass based detection of pcr amplicons
WO2024003260A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis
WO2024018485A1 (en) * 2022-07-18 2024-01-25 Haystackanalytics Private Limited Methods and systems for detection and identification of pathogens and antibiotic resistance genes
WO2024042042A1 (en) 2022-08-24 2024-02-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting monkeypox virus

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5256549A (en) * 1986-03-28 1993-10-26 Chiron Corporation Purification of synthetic oligomers
US5459255A (en) * 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
ATE176002T1 (de) * 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
CA2159631A1 (en) * 1993-03-30 1994-10-13 Sanofi Acyclic nucleoside analogs and oligonucleotide sequences containing them
US5599662A (en) * 1995-02-17 1997-02-04 Hoffmann-La Roche Inc. Oliconucleotide primers and probes for the detection of HIV-1
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid

Also Published As

Publication number Publication date
EP0866071A2 (de) 1998-09-23
PL325439A1 (en) 1998-09-28
CA2229766C (en) 2001-12-25
IL123694A (en) 2003-06-24
DK0866071T3 (da) 2005-01-17
DE69827060T2 (de) 2005-03-24
AU5840498A (en) 1998-10-01
US6001611A (en) 1999-12-14
NO322136B1 (no) 2006-08-21
CZ291525B6 (cs) 2003-03-12
CZ84198A3 (cs) 1998-10-14
KR19980080494A (ko) 1998-11-25
ES2230631T3 (es) 2005-05-01
BR9801878B1 (pt) 2010-05-18
AU712448B2 (en) 1999-11-04
BR9801878A (pt) 2000-09-19
EP0866071A3 (de) 1999-04-28
PL190142B1 (pl) 2005-11-30
HK1012192A1 (en) 1999-07-30
HU223669B1 (hu) 2004-11-29
HUP9800581A2 (hu) 1999-05-28
IL123694A0 (en) 1998-10-30
RU2159248C2 (ru) 2000-11-20
TW567188B (en) 2003-12-21
UA49843C2 (uk) 2002-10-15
NO981239D0 (no) 1998-03-19
NO981239L (no) 1998-09-21
HU9800581D0 (en) 1998-05-28
EP0866071B1 (de) 2004-10-20
CN1065873C (zh) 2001-05-16
DE69827060D1 (de) 2004-11-25
HUP9800581A3 (en) 2000-12-28
CA2229766A1 (en) 1998-09-20
JP2966389B2 (ja) 1999-10-25
JPH10279593A (ja) 1998-10-20
KR100292997B1 (ko) 2001-12-17
CN1194270A (zh) 1998-09-30

Similar Documents

Publication Publication Date Title
ATE280177T1 (de) Modifizierte primer
DE60115811D1 (de) Amplifizierung unter Verwendung von modifizierten Primern
DE60038796D1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
CA2153898A1 (en) Method for generating single-stranded dna molecules
AU6846698A (en) Method of nucleic acid amplification
DK1300466T3 (da) En kaskade nukleinsyre amplifikationsreaktion
DE69638318D1 (de) Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren
ES2243007T3 (es) Sintesis quimica completa y sintesis de genes de genomas.
ATE266737T1 (de) Künstliche fehlhybridisierung
NO20020348L (no) Peptid acceptor ligeringsmetoder
BR0014182A (pt) Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas
FI103809B1 (fi) In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
AU6589094A (en) 7-deazapurine modified oligonucleotides
WO2002057487A3 (en) Suppression of non-specific nucleic acid amplification
DE69736840D1 (de) Synthese von nukleosiden und polynukleotiden
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
DE60023480D1 (de) Amplifikation und sequenzierung von primer-paaren und deren verwendung
ATE494375T1 (de) Frazzled nukleotidsequenzen, expressions- produkte, zusammensetzungen und verwendungen
Graziewicz et al. Inhibition of DNA synthesis on templates oxidized by hydroxyl radical generating system

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0866071

Country of ref document: EP