ATE280177T1 - Modifizierte primer - Google Patents

Modifizierte primer

Info

Publication number
ATE280177T1
ATE280177T1 AT98104461T AT98104461T ATE280177T1 AT E280177 T1 ATE280177 T1 AT E280177T1 AT 98104461 T AT98104461 T AT 98104461T AT 98104461 T AT98104461 T AT 98104461T AT E280177 T1 ATE280177 T1 AT E280177T1
Authority
AT
Austria
Prior art keywords
carried out
amplification product
modified primers
modified oligonucleotides
primers
Prior art date
Application number
AT98104461T
Other languages
English (en)
Inventor
Stephen Gordon Will
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE280177T1 publication Critical patent/ATE280177T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paints Or Removers (AREA)
  • Dental Preparations (AREA)
  • Saccharide Compounds (AREA)
AT98104461T 1997-03-20 1998-03-12 Modifizierte primer ATE280177T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4112797P 1997-03-20 1997-03-20

Publications (1)

Publication Number Publication Date
ATE280177T1 true ATE280177T1 (de) 2004-11-15

Family

ID=21914898

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98104461T ATE280177T1 (de) 1997-03-20 1998-03-12 Modifizierte primer

Country Status (20)

Country Link
US (1) US6001611A (de)
EP (1) EP0866071B1 (de)
JP (1) JP2966389B2 (de)
KR (1) KR100292997B1 (de)
CN (1) CN1065873C (de)
AT (1) ATE280177T1 (de)
AU (1) AU712448B2 (de)
BR (1) BR9801878B1 (de)
CA (1) CA2229766C (de)
CZ (1) CZ291525B6 (de)
DE (1) DE69827060T2 (de)
DK (1) DK0866071T3 (de)
ES (1) ES2230631T3 (de)
HU (1) HU223669B1 (de)
IL (1) IL123694A (de)
NO (1) NO322136B1 (de)
PL (1) PL190142B1 (de)
RU (1) RU2159248C2 (de)
TW (1) TW567188B (de)
UA (1) UA49843C2 (de)

Families Citing this family (207)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
JP2003514383A (ja) 1999-11-17 2003-04-15 ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング 磁性ガラス粒子、それらの製造方法、及びそれらの使用
JP2003516765A (ja) 1999-12-15 2003-05-20 ジェン−プローブ・インコーポレーテッド 鳥型結核菌(Mycobacteriumavium)複合体種の検出のための方法および組成物
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
AU781192B2 (en) * 1999-12-17 2005-05-12 Biomerieux Sa Nucleic acid amplification and detection of mycobacterium species
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
WO2001075139A1 (en) * 2000-04-03 2001-10-11 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
US6548251B1 (en) * 2000-09-05 2003-04-15 Fidelity Systems, Inc. Inhibition of molecular and biological processes using modified oligonucleotides
US20020031777A1 (en) * 2000-09-08 2002-03-14 Linda Starr-Spires Ultra yield amplification reaction
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US6664079B2 (en) 2000-10-06 2003-12-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
DE60115811T2 (de) * 2000-10-25 2006-08-03 Roche Diagnostics Gmbh Amplifizierung unter Verwendung von modifizierten Primern
EP1373574A4 (de) * 2001-03-30 2007-01-03 Ge Healthcare Bio Sciences Ab P450-einzelnukleotidpolymorphismus-biochipanalyse
RU2218414C2 (ru) * 2001-05-04 2003-12-10 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ интеграции множественных полимеразных реакций амплификации с последующим анализом амплифицированных последовательностей непосредственно на биочипе
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2002101041A1 (en) * 2001-06-12 2002-12-19 Takara Bio Inc. Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog
WO2003020983A1 (en) * 2001-08-30 2003-03-13 Virginia Commonwealth University Allele specific pcr for genotyping
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー/アクセプター部分が相補鎖上となる標的核酸検出のmet/fretに基づく方法
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
RU2240350C1 (ru) * 2003-03-11 2004-11-20 НИИ онкологии им. проф. Н.Н.Петрова Минздрава РФ Способ идентификации генных мутаций и полиморфизмов
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7517978B1 (en) 2004-04-14 2009-04-14 Applied Biosystems, Llc Modified oligonucleotides and applications thereof
US7408051B2 (en) 2004-04-14 2008-08-05 Applera Corporation Modified oligonucleotides and applications thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2006112818A2 (en) * 2005-04-14 2006-10-26 Applera Corporation 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes
US20090232771A1 (en) 2004-07-13 2009-09-17 Takeda Pharmaceutical Company Limited Method of controlling cell functions
WO2006029184A2 (en) * 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
EP1809765A2 (de) * 2004-11-04 2007-07-25 Roche Diagnostics GmbH Klassifizierung von aml (akuter myeloider leukämie)
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
RU2299240C2 (ru) * 2005-02-21 2007-05-20 Рамиль Ришадович Вафин Способ проведения пцр
US8067208B2 (en) 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
WO2007139723A1 (en) 2006-06-01 2007-12-06 Trilink Biotechnologies Chemicallymodified oligonucleotide primers for nucleic acid amplification
WO2007147110A2 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
ATE538216T1 (de) * 2006-07-31 2012-01-15 Wanli Bi Nukleinsäureverstärkung mithilfe eines reversibel modifizierten oligonukleotids
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (de) 2006-11-09 2009-09-23 XDX, Inc. Verfahren zur diagnose und überwachung des status von systemischem lupus erythematodes
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7893227B2 (en) 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
ES2725003T3 (es) * 2007-03-28 2019-09-18 Signal Diagnostics Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia
AU2008272421B2 (en) 2007-07-03 2014-09-04 Genaphora Ltd. Chimeric primers for improved nucleic acid amplification reactions
EP2952588A1 (de) 2007-08-06 2015-12-09 Orion Genomics, LLC Einzelnukleotid-polymorphismen zur bestimmung der allelspezifischen expression des igf2-gens
CA2639416C (en) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Diagnostic test for susceptibility to b-raf kinase inhibitors
CN101889096B (zh) * 2007-10-04 2015-10-21 联邦科学及工业研究组织 核酸扩增
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
EP3431615A3 (de) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit nichtfluoreszierenden, reversiblen nukleotidterminatoren und spaltbaren, etikettmodifizierten nukleotidterminatoren
WO2009051807A1 (en) 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
EA201100001A3 (ru) 2008-06-11 2013-03-29 Лейзеджен, Инк. Нуклеотиды и нуклеозиды и методы их использования в секвенировании днк
CN104732118B (zh) 2008-08-04 2017-08-22 纳特拉公司 等位基因调用和倍性调用的方法
EP2337865B1 (de) * 2008-10-20 2014-11-19 Roche Diagnostics GmbH Allelspezifische amplifikation, die einen primer mit modifiziertem nukleotid verwendet
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
EP2955234A1 (de) 2009-02-11 2015-12-16 Orion Genomics, LLC Kombinationen aus polymorphismen zur bestimmung von allelspezifischer expression von igf2
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
ES2640776T3 (es) 2009-09-30 2017-11-06 Natera, Inc. Métodos para denominar de forma no invasiva ploidía prenatal
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
AU2011255641A1 (en) 2010-05-18 2012-12-06 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US10179937B2 (en) 2014-04-21 2019-01-15 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
WO2011153254A2 (en) 2010-06-04 2011-12-08 Chronix Biomedical Prostate cancer associated circulating nucleic acid biomarkers
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
WO2012075005A1 (en) 2010-12-01 2012-06-07 Berry & Associates, Inc. Compounds for the synthetic introduction of n-alkyl nucleosides into dna oligonucleotides
US20120164641A1 (en) * 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
CA2821906C (en) 2010-12-22 2020-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
CA2852098C (en) 2011-10-21 2023-05-02 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
ES2413566B1 (es) 2011-12-14 2014-05-20 Fundación Pública Andaluza Progreso Y Salud Método de obtención de datos útiles para el diagnóstico y pronóstico de la hipoacusia neurosensorial.
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
KR101545848B1 (ko) * 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
EP2861757B1 (de) 2012-06-14 2019-11-06 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits für polymerasekettenreaktionen (pcr)
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
EP2722399A1 (de) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation
US9382581B2 (en) 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
EP2931922B1 (de) 2012-12-14 2018-10-17 Chronix Biomedical Personalisierte biomarker für krebs
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9828629B2 (en) 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
LT3004388T (lt) 2013-05-29 2019-01-25 Chronix Biomedical Donoro ne ląstelinės dnr aptikimas ir jos kiekio nustatymas organo transplantanto recipientų apytakoje
CA2920666A1 (en) 2013-08-12 2015-02-19 Genentech, Inc. Compositions and method for treating complement-associated conditions
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
WO2015048535A1 (en) 2013-09-27 2015-04-02 Natera, Inc. Prenatal diagnostic resting standards
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
WO2015052147A1 (en) 2013-10-09 2015-04-16 Roche Diagnostics Gmbh Methods and compositions for detecting mutation in the human ezh2 gene
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
JP2016538882A (ja) 2013-10-30 2016-12-15 グリーン ライフ バイオテック,エルエルシー カンキツグリーニング病のための病因定量システム及び治療方法
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
TWI769970B (zh) 2014-02-08 2022-07-11 美商建南德克公司 治療阿茲海默症之方法
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
HRP20200764T1 (hr) 2014-10-01 2020-07-24 Chronix Biomedical Metode za kvantifikaciju cirkulirajuće slobodne dnk
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
US9970062B2 (en) * 2015-08-06 2018-05-15 Roche Molecular Systems, Inc. Compositions and methods for detection of mycobacterium tuberculosis
US9382582B1 (en) * 2015-08-25 2016-07-05 Tracy Hayden Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction
EP3402880B1 (de) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophile dna-polymerase-mutanten
CA3017265C (en) 2016-03-11 2022-08-16 F. Hoffmann-La Roche Ag Compositions and methods for detection of zika virus
JP6681804B2 (ja) * 2016-03-30 2020-04-15 大研医器株式会社 変異プライマーの設計方法
WO2017169119A1 (ja) * 2016-03-30 2017-10-05 大研医器株式会社 変異プライマーの設計方法
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
US10612101B2 (en) * 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
EP3464617B1 (de) 2016-05-27 2021-04-07 Roche Diagnostics GmbH Zusammensetzungen und verfahren zur detektion von trichomonas vaginalis
US10934597B2 (en) * 2016-05-27 2021-03-02 Roche Molecular Systems, Inc. Compositions and methods for detection of trichomonas vaginalis
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
US10443095B2 (en) 2016-08-02 2019-10-15 Roche Molecular Systems, Inc. Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
US12275999B2 (en) 2016-11-10 2025-04-15 Talis Biomedical Corporation Polynucleotides for the amplification and detection of chlamydia trachomatis
WO2018089945A1 (en) 2016-11-10 2018-05-17 Slipchip Corporation Polynucleotides for the amplification and detection of neisseria gonorrhoeae
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
EP3585889A1 (de) 2017-02-21 2020-01-01 Natera, Inc. Zusammensetzungen, verfahren und kits zur isolierung von nukleinsäuren
EP3645710A1 (de) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophile dna-polymerase-mutanten
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
US11591645B2 (en) * 2017-08-31 2023-02-28 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
CA3085933A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
WO2019161244A1 (en) 2018-02-15 2019-08-22 Natera, Inc. Methods for isolating nucleic acids with size selection
EP3768832B1 (de) 2018-03-21 2023-11-29 F. Hoffmann-La Roche AG Dna-polymerasen zum effizienten und effektiven einbau von methylierten dntps
EP3781714B1 (de) 2018-04-14 2026-01-07 Natera, Inc. Verfahren zur krebserkennung und -überwachung durch personalisierte detektion zirkulierender tumor-dna
US10450616B1 (en) 2018-05-09 2019-10-22 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Chlamydia trachomatis
WO2020006421A1 (en) 2018-06-29 2020-01-02 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN113227398B (zh) * 2018-10-29 2025-03-14 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
ES3031673T3 (en) 2018-12-03 2025-07-10 Hoffmann La Roche Compositions and methods for detection of candida auris
WO2020132279A1 (en) 2018-12-19 2020-06-25 Nuclein, Llc Apparatus and methods for molecular diagnostics
KR102141312B1 (ko) * 2019-04-19 2020-08-04 주식회사 제노헬릭스 짧은 RNA-primed 제노 센서 모듈 증폭 기반 짧은 RNA 탐지 기법
JP7628085B2 (ja) 2019-05-02 2025-02-07 エフ. ホフマン-ラ ロシュ アーゲー 鎖分離温度に基づくDNAに対してRNA標的の優先的/選択的増幅のためのdITPの利用
CN113795596A (zh) 2019-05-07 2021-12-14 豪夫迈·罗氏有限公司 用于检测淋病奈瑟氏球菌的组合物和方法
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
US10954572B2 (en) 2019-07-25 2021-03-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Neisseria gonorrhoeae
JP7649285B2 (ja) 2019-07-25 2025-03-19 エフ. ホフマン-ラ ロシュ アーゲー エプスタイン・バーウイルス(ebv)を検出するための組成物および方法
US11739382B2 (en) 2019-08-27 2023-08-29 Roche Molecular Systems, Inc. Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
CN114867871A (zh) 2019-12-27 2022-08-05 豪夫迈·罗氏有限公司 用于检测耐甲氧西林金黄色葡萄球菌的组合物和方法
US20220356537A1 (en) 2019-12-31 2022-11-10 Roche Molecular Systems, Inc. Quantitative pcr screening of inducible prophage from bacterial isolates
JP7796660B2 (ja) 2020-03-09 2026-01-09 エフ. ホフマン-ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス2(sars-cov-2)、インフルエンザa及びインフルエンザbを検出するための組成物及び方法
US12264365B2 (en) 2020-03-23 2025-04-01 Talis Biomedical Corporation Polynucleotides for amplification and detection of SARS-CoV-2
WO2022029157A2 (en) 2020-08-06 2022-02-10 F. Hoffmann-La Roche Ag Compositions and methods for the detection of severe acute respiratory syndrome coronavirus 2 (sars-2), influenza a, and influenza b
EP4256089A1 (de) 2020-12-04 2023-10-11 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis von malaria
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
EP4288567A1 (de) 2021-02-05 2023-12-13 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis des menschlichen parainfluenzavirus 1-4- (hpiv1-4)
US20220290221A1 (en) * 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
CN117858960A (zh) 2021-05-06 2024-04-09 豪夫迈·罗氏有限公司 用于通过双靶标测定来检测丁型肝炎病毒的组合物和方法
EP4426865A1 (de) 2021-11-05 2024-09-11 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis von malaria
WO2023089186A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance
EP4444904A1 (de) 2021-12-09 2024-10-16 F. Hoffmann-La Roche AG Massenbasierter nachweis von pcr-amplikons
WO2023109887A1 (zh) 2021-12-15 2023-06-22 南京金斯瑞生物科技有限公司 一种整合位点的检测方法
JP2025521750A (ja) 2022-06-30 2025-07-10 エフ. ホフマン-ラ ロシュ アーゲー クラミジア・トラコマチス(chlamydia trachomatis)の鼠径リンパ肉芽腫症(lgv)血清型を検出するための組成物および方法
EP4558648A1 (de) * 2022-07-18 2025-05-28 Haystackanalytics Private Limited Verfahren und systeme zum nachweis und zur identifizierung von pathogenen und antibiotikaresistenzgenen
EP4577674A1 (de) 2022-08-24 2025-07-02 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis des affektoxvirus
JP2025542121A (ja) 2022-12-25 2025-12-25 エフ. ホフマン-ラ ロシュ アーゲー カルバペネム耐性アシネトバクター・カルコアセチカス-バウマンニ(acinetobacter calcoaceticus-baumannii)(crab)を検出するための組成物および方法
EP4642924A1 (de) 2022-12-28 2025-11-05 F. Hoffmann-La Roche AG Digitale pcr-testdesigns für mehrere hepatitis-b-virus-genziele und nicht-dehnbare blockeroligonukleotide dafür
EP4669777A1 (de) 2023-02-25 2025-12-31 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis von candida-spezies
WO2024184165A1 (en) 2023-03-03 2024-09-12 F. Hoffmann-La Roche Ag Compositions and methods for the detection of group b streptococcus ( streptococcus agalactiae) and clindamycin resistance gene determinants
WO2025024676A1 (en) 2023-07-26 2025-01-30 Caribou Biosciences, Inc. In vitro validation methods for cd19-targeting cell therapies
WO2025027080A1 (en) 2023-08-03 2025-02-06 F. Hoffmann-La Roche Ag Use of 5'-tailed long primers to improve amplification performance when targeting short primer-binding sites
WO2025202488A1 (en) 2024-03-28 2025-10-02 Roche Diagnostics Gmbh Enhancer of nucleic acid amplification
WO2026052826A1 (en) 2024-09-09 2026-03-12 Roche Diagnostics Gmbh Methods for producing fluorescent dyes

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5256549A (en) * 1986-03-28 1993-10-26 Chiron Corporation Purification of synthetic oligomers
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5354656A (en) * 1989-10-02 1994-10-11 Stratagene Method of DNA sequencing
US5459255A (en) * 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
DK0540693T3 (da) * 1990-07-24 1999-09-13 Hoffmann La Roche Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
GB9024005D0 (en) * 1990-11-05 1990-12-19 British Bio Technology Process for amplifying nucleic acid
WO1993005175A1 (en) * 1991-09-11 1993-03-18 Medical Research Council Improvements in oligonucleotide primers and probes
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
AU6449394A (en) * 1993-03-30 1994-10-24 Sterling Winthrop Inc. Acyclic nucleoside analogs and oligonucleotide sequences containing them
RU2084534C1 (ru) * 1993-07-07 1997-07-20 Институт молекулярной генетики РАН Способ получения двуцепочечной нуклеиновой кислоты
FR2722799B1 (fr) * 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
US5599662A (en) * 1995-02-17 1997-02-04 Hoffmann-La Roche Inc. Oliconucleotide primers and probes for the detection of HIV-1
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid

Also Published As

Publication number Publication date
JP2966389B2 (ja) 1999-10-25
IL123694A (en) 2003-06-24
CZ84198A3 (cs) 1998-10-14
CZ291525B6 (cs) 2003-03-12
DK0866071T3 (da) 2005-01-17
HU223669B1 (hu) 2004-11-29
EP0866071A2 (de) 1998-09-23
NO981239D0 (no) 1998-03-19
NO981239L (no) 1998-09-21
BR9801878B1 (pt) 2010-05-18
HUP9800581A2 (hu) 1999-05-28
EP0866071B1 (de) 2004-10-20
TW567188B (en) 2003-12-21
PL325439A1 (en) 1998-09-28
EP0866071A3 (de) 1999-04-28
CN1065873C (zh) 2001-05-16
AU712448B2 (en) 1999-11-04
BR9801878A (pt) 2000-09-19
ES2230631T3 (es) 2005-05-01
CN1194270A (zh) 1998-09-30
DE69827060T2 (de) 2005-03-24
AU5840498A (en) 1998-10-01
IL123694A0 (en) 1998-10-30
PL190142B1 (pl) 2005-11-30
RU2159248C2 (ru) 2000-11-20
UA49843C2 (uk) 2002-10-15
HK1012192A1 (en) 1999-07-30
KR100292997B1 (ko) 2001-12-17
DE69827060D1 (de) 2004-11-25
HUP9800581A3 (en) 2000-12-28
HU9800581D0 (en) 1998-05-28
JPH10279593A (ja) 1998-10-20
US6001611A (en) 1999-12-14
CA2229766A1 (en) 1998-09-20
NO322136B1 (no) 2006-08-21
KR19980080494A (ko) 1998-11-25
CA2229766C (en) 2001-12-25

Similar Documents

Publication Publication Date Title
DE69827060D1 (de) Modifizierte Primer
ATE312942T1 (de) Amplifizierung unter verwendung von modifizierten primern
ATE394410T1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
DK0868530T3 (da) Kaskade-nucleinsyreamplificeringsreaktion
CA2153898A1 (en) Method for generating single-stranded dna molecules
AU6846698A (en) Method of nucleic acid amplification
DE69638318D1 (de) Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren
ES2243007T3 (es) Sintesis quimica completa y sintesis de genes de genomas.
DE69403642D1 (de) 7-deazapurin modifizierte oligonukleotide
ATE266737T1 (de) Künstliche fehlhybridisierung
NO20020348L (no) Peptid acceptor ligeringsmetoder
BR0014182A (pt) Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas
FI103809B1 (fi) In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
WO2002057487A3 (en) Suppression of non-specific nucleic acid amplification
ATE342911T1 (de) Synthese von nukleosiden und polynukleotiden
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
ATE307901T1 (de) Amplifikation und sequenzierung von primer-paaren und deren verwendung
WO2002048401A3 (en) Nested oligonucleotides containing hairpin for nucleic acid amplification
Graziewicz et al. Inhibition of DNA synthesis on templates oxidized by hydroxyl radical generating system

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0866071

Country of ref document: EP