DK0866071T3 - Modificerede primere - Google Patents

Modificerede primere

Info

Publication number
DK0866071T3
DK0866071T3 DK98104461T DK98104461T DK0866071T3 DK 0866071 T3 DK0866071 T3 DK 0866071T3 DK 98104461 T DK98104461 T DK 98104461T DK 98104461 T DK98104461 T DK 98104461T DK 0866071 T3 DK0866071 T3 DK 0866071T3
Authority
DK
Denmark
Prior art keywords
carried out
amplification product
modified primers
modified oligonucleotides
primers
Prior art date
Application number
DK98104461T
Other languages
Danish (da)
English (en)
Inventor
Stephen Gordon Will
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0866071T3 publication Critical patent/DK0866071T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DK98104461T 1997-03-20 1998-03-12 Modificerede primere DK0866071T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4112797P 1997-03-20 1997-03-20
EP98104461A EP0866071B1 (de) 1997-03-20 1998-03-12 Modifizierte Primer

Publications (1)

Publication Number Publication Date
DK0866071T3 true DK0866071T3 (da) 2005-01-17

Family

ID=21914898

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98104461T DK0866071T3 (da) 1997-03-20 1998-03-12 Modificerede primere

Country Status (21)

Country Link
US (1) US6001611A (de)
EP (1) EP0866071B1 (de)
JP (1) JP2966389B2 (de)
KR (1) KR100292997B1 (de)
CN (1) CN1065873C (de)
AT (1) ATE280177T1 (de)
AU (1) AU712448B2 (de)
BR (1) BR9801878B1 (de)
CA (1) CA2229766C (de)
CZ (1) CZ291525B6 (de)
DE (1) DE69827060T2 (de)
DK (1) DK0866071T3 (de)
ES (1) ES2230631T3 (de)
HK (1) HK1012192A1 (de)
HU (1) HU223669B1 (de)
IL (1) IL123694A (de)
NO (1) NO322136B1 (de)
PL (1) PL190142B1 (de)
RU (1) RU2159248C2 (de)
TW (1) TW567188B (de)
UA (1) UA49843C2 (de)

Families Citing this family (181)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6200757B1 (en) * 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
SK287932B6 (sk) 1999-11-17 2012-05-03 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
JP2003516765A (ja) 1999-12-15 2003-05-20 ジェン−プローブ・インコーポレーテッド 鳥型結核菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍａｖｉｕｍ）複合体種の検出のための方法および組成物
WO2001044510A2 (en) * 1999-12-17 2001-06-21 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
AU2001253129A1 (en) * 2000-04-03 2001-10-15 Biolink Partners, Inc. Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
US6548251B1 (en) * 2000-09-05 2003-04-15 Fidelity Systems, Inc. Inhibition of molecular and biological processes using modified oligonucleotides
WO2002020845A2 (en) * 2000-09-08 2002-03-14 Thomas Jefferson University Ultra yield amplification reaction
US6664079B2 (en) 2000-10-06 2003-12-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE312942T1 (de) * 2000-10-25 2005-12-15 Hoffmann La Roche Amplifizierung unter verwendung von modifizierten primern
EP1373574A4 (de) * 2001-03-30 2007-01-03 Ge Healthcare Bio Sciences Ab P450-einzelnukleotidpolymorphismus-biochipanalyse
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2002101041A1 (fr) * 2001-06-12 2002-12-19 Takara Bio Inc. Procede d'amplification de l'acide nucleique et procede de detection du polymorphisme des nucleotides a l'aide d'un analogue de nucleotide
US20030096277A1 (en) * 2001-08-30 2003-05-22 Xiangning Chen Allele specific PCR for genotyping
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
EP1509624A2 (de) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Methode für den nukleinsäurenachweis basierend auf met/fret wobei sich die fluorophoren gruppen auf komplementären strängen befinden
US7955795B2 (en) 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
JP4782668B2 (ja) * 2003-03-31 2011-09-28 エフ．ホフマン−ラ ロシュ アーゲー 日本脳炎ウィルス血清グループのメンバーを包含する一定のフラビウィルスを検出するための組成物及び方法
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7517978B1 (en) 2004-04-14 2009-04-14 Applied Biosystems, Llc Modified oligonucleotides and applications thereof
US7408051B2 (en) 2004-04-14 2008-08-05 Applera Corporation Modified oligonucleotides and applications thereof
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20090232771A1 (en) 2004-07-13 2009-09-17 Takeda Pharmaceutical Company Limited Method of controlling cell functions
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
EP1809765A2 (de) * 2004-11-04 2007-07-25 Roche Diagnostics GmbH Klassifizierung von aml (akuter myeloider leukämie)
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
EP2409980B1 (de) * 2005-04-14 2015-06-03 Applied Biosystems, LLC 3'-modifizierte Oligonucleotide mit Pseudoisocytosin-Nukleobasederivaten und Anwendungen daraus als Primer oder Sonden
US8067208B2 (en) 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
WO2007139723A1 (en) 2006-06-01 2007-12-06 Trilink Biotechnologies Chemicallymodified oligonucleotide primers for nucleic acid amplification
AU2007260707A1 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
ATE538216T1 (de) * 2006-07-31 2012-01-15 Wanli Bi Nukleinsäureverstärkung mithilfe eines reversibel modifizierten oligonukleotids
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US7893227B2 (en) 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
CN101903535A (zh) * 2007-03-28 2010-12-01 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
CN103320506B (zh) 2007-07-03 2015-10-28 吉纳珀莱有限公司 用于改进的核酸扩增反应的嵌合引物
WO2009021054A2 (en) 2007-08-06 2009-02-12 Orion Genomics Llc Novel single nucleotide polymorphisms and combinations of novel and known polymorphisms for determining the allele-specific expression of the igf2 gene
CA2639416C (en) 2007-09-11 2019-12-31 F. Hoffmann-La Roche Ag Diagnostic test for susceptibility to b-raf kinase inhibitors
WO2009043112A1 (en) * 2007-10-04 2009-04-09 Commonwealth Scientific And Industrial Research Organisation Nucleic acid amplification
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
EP4310194A2 (de) 2007-10-19 2024-01-24 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
WO2009135093A2 (en) 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2307565B1 (de) 2008-06-11 2017-11-29 Lasergen, Inc. Spaltbare markierte nukleoside und nukleotide und verfahren zu ihrer verwendung bei der dna-sequenzierung
CN104732118B (zh) 2008-08-04 2017-08-22 纳特拉公司 等位基因调用和倍性调用的方法
JP5624044B2 (ja) * 2008-10-20 2014-11-12 エフ．ホフマン−ラ ロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善されたアレル−特異的増幅
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
AU2010213727A1 (en) 2009-02-11 2011-09-29 Orion Genomics Llc Combinations of polymorphisms for determining allele-specific expression of IGF2
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
WO2011041485A1 (en) 2009-09-30 2011-04-07 Gene Security Network, Inc. Methods for non-invasive prenatal ploidy calling
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
WO2011128096A1 (en) 2010-04-16 2011-10-20 Roche Diagnostics Gmbh Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
AU2011255641A1 (en) 2010-05-18 2012-12-06 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
WO2011153254A2 (en) 2010-06-04 2011-12-08 Chronix Biomedical Prostate cancer associated circulating nucleic acid biomarkers
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
WO2012075005A1 (en) 2010-12-01 2012-06-07 Berry & Associates, Inc. Compounds for the synthetic introduction of n-alkyl nucleosides into dna oligonucleotides
US20120164641A1 (en) * 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
RU2620959C2 (ru) 2010-12-22 2017-05-30 Натера, Инк. Способы неинвазивного пренатального установления отцовства
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
CA2852098C (en) 2011-10-21 2023-05-02 Chronix Biomedical Colorectal cancer associated circulating nucleic acid biomarkers
ES2413566B1 (es) 2011-12-14 2014-05-20 Fundación Pública Andaluza Progreso Y Salud Método de obtención de datos útiles para el diagnóstico y pronóstico de la hipoacusia neurosensorial.
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
KR101545848B1 (ko) * 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
EP2861757B1 (de) 2012-06-14 2019-11-06 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits für polymerasekettenreaktionen (pcr)
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
EP2722399A1 (de) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation
US9382581B2 (en) * 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
EP2931922B1 (de) 2012-12-14 2018-10-17 Chronix Biomedical Personalisierte biomarker für krebs
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9828629B2 (en) 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
PL3004388T5 (pl) 2013-05-29 2023-10-09 Chronix Biomedical Wykrywanie i oznaczanie ilościowe pozakomórkowego dna dawcy w układzie krążenia biorców przeszczepów organów
CN107318267B (zh) 2013-08-12 2021-08-17 豪夫迈·罗氏有限公司 用于治疗补体相关的病症的组合物和方法
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
CN105793435A (zh) 2013-10-09 2016-07-20 弗洛雷森特里克公司 多重探针
CN105593378B (zh) 2013-10-09 2019-09-20 豪夫迈·罗氏有限公司 用于在人ezh2基因中检测突变的方法和组合物
CN105722977A (zh) 2013-10-30 2016-06-29 绿色生活生物技术有限责任公司 用于柑橘绿化枯萎病的发病定量系统和治疗方法
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
BR112016018205A8 (pt) 2014-02-08 2018-04-17 Genentech Inc métodos de tratamento de mal de alzheimer, de seleção de pacientes, de identificação de pacientes, de previsão, de otimização da eficácia terapêutica, kit, uso de agente e uso in vitro de agente
CN109971852A (zh) 2014-04-21 2019-07-05 纳特拉公司 检测染色体片段中的突变和倍性
US10570443B2 (en) 2014-10-01 2020-02-25 Chronix Biomedical Methods of quantifying cell-free DNA
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
US9920381B2 (en) * 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
CA2971589C (en) 2014-12-18 2021-09-28 Edico Genome Corporation Chemically-sensitive field effect transistor
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
US9970062B2 (en) * 2015-08-06 2018-05-15 Roche Molecular Systems, Inc. Compositions and methods for detection of mycobacterium tuberculosis
US9382582B1 (en) * 2015-08-25 2016-07-05 Tracy Hayden Methods, compositions and kits for enriching for a minor template amplification product in a mixed template amplification reaction
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
CN108779500B (zh) 2016-03-11 2022-12-13 豪夫迈·罗氏有限公司 用于检测寨卡病毒的组合物和方法
JP6681804B2 (ja) * 2016-03-30 2020-04-15 大研医器株式会社 変異プライマーの設計方法
WO2017169119A1 (ja) * 2016-03-30 2017-10-05 大研医器株式会社 変異プライマーの設計方法
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10612101B2 (en) * 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
US10934597B2 (en) * 2016-05-27 2021-03-02 Roche Molecular Systems, Inc. Compositions and methods for detection of trichomonas vaginalis
ES2874259T3 (es) 2016-05-27 2021-11-04 Roche Diagnostics Gmbh Composiciones y procedimientos para la detección de Trichomonas vaginalis
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
US10443095B2 (en) 2016-08-02 2019-10-15 Roche Molecular Systems, Inc. Helper oligonucleotide for improved efficiency of amplification and detection/quantitation of nucleic acids
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
CN110177887A (zh) 2016-11-10 2019-08-27 达丽斯生物医学公司 用于扩增和检测沙眼衣原体的多核苷酸
WO2018089945A1 (en) 2016-11-10 2018-05-17 Slipchip Corporation Polynucleotides for the amplification and detection of neisseria gonorrhoeae
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
EP3585889A1 (de) 2017-02-21 2020-01-01 Natera, Inc. Zusammensetzungen, verfahren und kits zur isolierung von nukleinsäuren
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
US10760137B2 (en) * 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
WO2019045532A2 (en) * 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
WO2019180137A1 (en) 2018-03-21 2019-09-26 F. Hoffmann-La Roche Ag Dna polymerases for efficient and effective incorporation of methylated-dntps
US10450616B1 (en) 2018-05-09 2019-10-22 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Chlamydia trachomatis
EP3814531A4 (de) 2018-06-29 2022-04-06 Pacific Biosciences Of California, Inc. Verfahren und zusammensetzungen zur verabreichung von molekülen und komplexen an reaktionsstellen
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CA3118000A1 (en) * 2018-10-29 2020-05-07 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
JP7478734B2 (ja) 2018-12-03 2024-05-07 エフ． ホフマン－ラ ロシュ アーゲー カンジダ・アウリス（ｃａｎｄｉｄａ ａｕｒｉｓ）の検出のための組成物および方法
KR102141312B1 (ko) * 2019-04-19 2020-08-04 주식회사 제노헬릭스 짧은 RNA-primed 제노 센서 모듈 증폭 기반 짧은 RNA 탐지 기법
CN113785073A (zh) 2019-05-02 2021-12-10 豪夫迈·罗氏有限公司 基于链分离温度利用dITP进行RNA靶标相比于DNA靶标的优先性/选择性扩增
JP2022531881A (ja) 2019-05-07 2022-07-12 エフ．ホフマン－ラ ロシュ アーゲー ナイセリア・ゴノレア（Ｎｅｉｓｓｅｒｉａ Ｇｏｎｏｒｒｏｈｅａｅ）を検出するための組成物および方法
US10954572B2 (en) 2019-07-25 2021-03-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Neisseria gonorrhoeae
US20220251670A1 (en) 2019-07-25 2022-08-11 Roche Molecular Systems, Inc. Compositions and methods for detection of epstein barr virus (ebv)
US11739382B2 (en) 2019-08-27 2023-08-29 Roche Molecular Systems, Inc. Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
JP2023508100A (ja) 2019-12-27 2023-02-28 エフ． ホフマン－ラ ロシュ エージー． メチシリン耐性黄色ブドウ球菌を検出するための組成物および方法
US20220356537A1 (en) 2019-12-31 2022-11-10 Roche Molecular Systems, Inc. Quantitative pcr screening of inducible prophage from bacterial isolates
US20210285061A1 (en) 2020-03-09 2021-09-16 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2), influenza a and influenza b
WO2022029157A2 (en) 2020-08-06 2022-02-10 F. Hoffmann-La Roche Ag Compositions and methods for the detection of severe acute respiratory syndrome coronavirus 2 (sars-2), influenza a, and influenza b
WO2022117784A1 (en) 2020-12-04 2022-06-09 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
CN116806268A (zh) 2021-02-05 2023-09-26 豪夫迈·罗氏有限公司 用于检测人副流感病毒1-4(hpiv 1-4)的组合物和方法
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
WO2022233930A1 (en) 2021-05-06 2022-11-10 F. Hoffmann-La Roche Ag Compositions and methods for detecting hepatitis delta virus by a dual-target assay
WO2023079032A1 (en) 2021-11-05 2023-05-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
WO2023089186A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance
WO2023104812A1 (en) 2021-12-09 2023-06-15 F. Hoffmann-La Roche Ag Mass based detection of pcr amplicons
WO2024003260A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis
WO2024018485A1 (en) * 2022-07-18 2024-01-25 Haystackanalytics Private Limited Methods and systems for detection and identification of pathogens and antibiotic resistance genes
WO2024042042A1 (en) 2022-08-24 2024-02-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting monkeypox virus

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5256549A (en) * 1986-03-28 1993-10-26 Chiron Corporation Purification of synthetic oligomers
US5459255A (en) * 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
EP0540693B1 (de) * 1990-07-24 1999-01-20 F. Hoffmann-La Roche Ag Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
EP0691968B1 (de) * 1993-03-30 1997-07-16 Sanofi Acyclische nucleosid analoge und sie enthaltende oligonucleotidsequenzen
US5599662A (en) * 1995-02-17 1997-02-04 Hoffmann-La Roche Inc. Oliconucleotide primers and probes for the detection of HIV-1
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid

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DE69827060D1 (de) 2004-11-25
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KR100292997B1 (ko) 2001-12-17
HU223669B1 (hu) 2004-11-29
CZ84198A3 (cs) 1998-10-14
DE69827060T2 (de) 2005-03-24
US6001611A (en) 1999-12-14
UA49843C2 (uk) 2002-10-15
HU9800581D0 (en) 1998-05-28
HUP9800581A2 (hu) 1999-05-28
ES2230631T3 (es) 2005-05-01
JP2966389B2 (ja) 1999-10-25
EP0866071A3 (de) 1999-04-28
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CA2229766A1 (en) 1998-09-20
ATE280177T1 (de) 2004-11-15
AU712448B2 (en) 1999-11-04
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KR19980080494A (ko) 1998-11-25
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AU5840498A (en) 1998-10-01
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