ATE454476T1 - 3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sonden - Google Patents
3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sondenInfo
- Publication number
- ATE454476T1 ATE454476T1 AT05735457T AT05735457T ATE454476T1 AT E454476 T1 ATE454476 T1 AT E454476T1 AT 05735457 T AT05735457 T AT 05735457T AT 05735457 T AT05735457 T AT 05735457T AT E454476 T1 ATE454476 T1 AT E454476T1
- Authority
- AT
- Austria
- Prior art keywords
- primers
- pseudoisocytosine
- probes
- applications
- modified oligonucleotides
- Prior art date
Links
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Chemical class Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 239000000523 sample Substances 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000000539 dimer Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2005/012570 WO2006112818A2 (en) | 2005-04-14 | 2005-04-14 | 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE454476T1 true ATE454476T1 (de) | 2010-01-15 |
Family
ID=36570763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05735457T ATE454476T1 (de) | 2005-04-14 | 2005-04-14 | 3'-modifizierte oligonukleotide mit pseudoisocytosin-nukleobasenderivaten sowie deren anwendungen als primer oder sonden |
Country Status (6)
| Country | Link |
|---|---|
| EP (2) | EP2409980B1 (de) |
| JP (1) | JP2008535518A (de) |
| CN (1) | CN101171343A (de) |
| AT (1) | ATE454476T1 (de) |
| DE (1) | DE602005018840D1 (de) |
| WO (1) | WO2006112818A2 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2647237T3 (es) | 2007-07-03 | 2017-12-20 | Genaphora Ltd. | Cebadores quiméricos para reacciones de amplificación de ácidos nucleicos mejoradas |
| US8501441B2 (en) | 2008-03-10 | 2013-08-06 | Quantibact A/S | Target amplification and sequencing with primers comprising triplex forming monomer units |
| EP2867377B1 (de) | 2012-07-02 | 2019-08-21 | The Translational Genomics Research Institute | Primer, assays und verfahren zum nachweis eines e.-coli-subtyps |
| CN105555971B (zh) * | 2013-08-09 | 2017-08-29 | 卢米耐克斯公司 | 用于在核酸测定中改善熔链分辨和多重性的探针 |
| WO2017169119A1 (ja) * | 2016-03-30 | 2017-10-05 | 大研医器株式会社 | 変異プライマーの設計方法 |
| JP6681804B2 (ja) * | 2016-03-30 | 2020-04-15 | 大研医器株式会社 | 変異プライマーの設計方法 |
| AU2017268369B2 (en) * | 2016-05-18 | 2022-09-01 | Integrated Nano-Technologies, Inc. | Method for detection of a PCR product |
| FR3110602B1 (fr) | 2020-05-20 | 2025-03-07 | Anova Plus | Methode de detection sur le terrain de la presence et de la mutliresistance d’un bio-agresseur, notamment dezymo-septoria tritici,dans des culturescerealieres |
| FR3129407B1 (fr) | 2021-11-25 | 2024-12-13 | Anova Plus | Methode de detection de la presence d’un pathogene et determination de ses caracteristiques en sante humaine et animale |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US3956302A (en) | 1974-05-24 | 1976-05-11 | The Upjohn Company | Substituted pyrimidines |
| US4318846A (en) | 1979-09-07 | 1982-03-09 | Syva Company | Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers |
| US4577018A (en) | 1983-08-22 | 1986-03-18 | American Cyanamid Company | 2,3-Diamino-substituted-4(3H)-pyrimidinones and platinum chelates |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US4811218A (en) | 1986-06-02 | 1989-03-07 | Applied Biosystems, Inc. | Real time scanning electrophoresis apparatus for DNA sequencing |
| JPS641949A (en) | 1987-06-24 | 1989-01-06 | Hitachi Ltd | Regeneration of electrophoretic gel |
| US5585481A (en) | 1987-09-21 | 1996-12-17 | Gen-Probe Incorporated | Linking reagents for nucleotide probes |
| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5451463A (en) | 1989-08-28 | 1995-09-19 | Clontech Laboratories, Inc. | Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides |
| US5274240A (en) | 1990-01-12 | 1993-12-28 | The Regents Of The University Of California | Capillary array confocal fluorescence scanner and method |
| US5091652A (en) | 1990-01-12 | 1992-02-25 | The Regents Of The University Of California | Laser excited confocal microscope fluorescence scanner and method |
| US5714331A (en) | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5766855A (en) | 1991-05-24 | 1998-06-16 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity and sequence specificity |
| US5419966A (en) | 1991-06-10 | 1995-05-30 | Microprobe Corporation | Solid support for synthesis of 3'-tailed oligonucleotides |
| DK0519338T3 (da) | 1991-06-20 | 1996-10-28 | Hoffmann La Roche | Forbedrede fremgangsmåder til nukleinsyreamplifikation |
| US5512677A (en) | 1991-08-13 | 1996-04-30 | National Science Council | 3-substituted methyl-2,3-dihydroimidazo 1,2-c!quinazoline derivatives, the preparation and use thereof |
| WO1993020236A1 (en) | 1992-04-03 | 1993-10-14 | Applied Biosystems, Inc. | Probe composition and method |
| US5374527A (en) | 1993-01-21 | 1994-12-20 | Applied Biosystems, Inc. | High resolution DNA sequencing method using low viscosity medium |
| EP0695305B1 (de) | 1993-04-12 | 2003-08-06 | Northwestern University | Verfahren zur darstellung von oligonukleotiden |
| AU1254295A (en) | 1993-12-17 | 1995-07-03 | Perkin-Elmer Corporation, The | Uncharged polymers for separation of biomolecules by capillary electrophoresis |
| US5543026A (en) | 1994-02-07 | 1996-08-06 | The Perkin-Elmer Corporation | Real-time scanning fluorescence electrophoresis apparatus for the analysis of polynucleotide fragments |
| US5801155A (en) | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
| US5736626A (en) | 1996-01-29 | 1998-04-07 | The Perkin-Elmer Corporation | Solid support reagents for the direct synthesis of 3'-labeled polynucleotides |
| ATE280177T1 (de) * | 1997-03-20 | 2004-11-15 | Hoffmann La Roche | Modifizierte primer |
| IL135000A0 (en) | 1997-09-12 | 2001-05-20 | Exiqon As | Bi- and tri-cyclic nucleoside, nucleotide and oligonucleotide analogues |
| US5936087A (en) | 1997-11-25 | 1999-08-10 | The Perkin-Elmer Corporation | Dibenzorhodamine dyes |
| US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| US6287772B1 (en) * | 1998-04-29 | 2001-09-11 | Boston Probes, Inc. | Methods, kits and compositions for detecting and quantitating target sequences |
| US6432642B1 (en) * | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
| CA2335359C (en) | 1999-04-23 | 2007-07-17 | Molecular Probes, Inc. | Xanthene dyes and their application as luminescence quenching compounds |
| US6316230B1 (en) * | 1999-08-13 | 2001-11-13 | Applera Corporation | Polymerase extension at 3′ terminus of PNA-DNA chimera |
| AU2582600A (en) | 1999-09-16 | 2001-04-17 | Rimma Iliinichna Ashkinazi | Bioactive substance containing derivatives of 2-amino-6-aryloxypyrimidines and intermediary products of synthesis thereof |
| US6297016B1 (en) * | 1999-10-08 | 2001-10-02 | Applera Corporation | Template-dependent ligation with PNA-DNA chimeric probes |
| JP2003528627A (ja) * | 2000-03-27 | 2003-09-30 | アプレラ コーポレイション | 改善された侵襲アッセイ |
| US6887664B2 (en) | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
| ATE348191T1 (de) * | 2000-07-03 | 2007-01-15 | Applera Corp | Polynukleotid-sequenzassay |
| EP1448800A4 (de) * | 2001-11-21 | 2007-05-16 | Applera Corp | Digitaler assay |
| US20040171047A1 (en) * | 2002-05-22 | 2004-09-02 | Dahl Gary A. | Target-dependent transcription |
| JP2007516695A (ja) * | 2003-06-12 | 2007-06-28 | アプレラ コーポレイション | ユニバーサル塩基アナログを含む組合せ核酸塩基オリゴマーならびにこれらを作製する方法および使用する方法 |
-
2005
- 2005-04-14 AT AT05735457T patent/ATE454476T1/de not_active IP Right Cessation
- 2005-04-14 EP EP10000030.6A patent/EP2409980B1/de not_active Expired - Lifetime
- 2005-04-14 DE DE602005018840T patent/DE602005018840D1/de not_active Expired - Lifetime
- 2005-04-14 WO PCT/US2005/012570 patent/WO2006112818A2/en not_active Ceased
- 2005-04-14 CN CNA2005800497013A patent/CN101171343A/zh active Pending
- 2005-04-14 EP EP05735457A patent/EP1902142B1/de not_active Ceased
- 2005-04-14 JP JP2008506424A patent/JP2008535518A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1902142B1 (de) | 2010-01-06 |
| EP2409980A1 (de) | 2012-01-25 |
| DE602005018840D1 (de) | 2010-02-25 |
| CN101171343A (zh) | 2008-04-30 |
| JP2008535518A (ja) | 2008-09-04 |
| EP2409980B1 (de) | 2015-06-03 |
| WO2006112818A2 (en) | 2006-10-26 |
| WO2006112818A3 (en) | 2007-01-25 |
| EP1902142A2 (de) | 2008-03-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |