DE60042767D1 - Erhöhung der Spezifität von Nukleinsäureamplifizierungen durch Trägernukleinsäuren - Google Patents

Erhöhung der Spezifität von Nukleinsäureamplifizierungen durch Trägernukleinsäuren

Info

Publication number
DE60042767D1
DE60042767D1 DE60042767T DE60042767T DE60042767D1 DE 60042767 D1 DE60042767 D1 DE 60042767D1 DE 60042767 T DE60042767 T DE 60042767T DE 60042767 T DE60042767 T DE 60042767T DE 60042767 D1 DE60042767 D1 DE 60042767D1
Authority
DE
Germany
Prior art keywords
nucleic acid
carrier
specificity
increasing
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60042767T
Other languages
English (en)
Inventor
Gregory M Preston
John W Backus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Ortho Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Application granted granted Critical
Publication of DE60042767D1 publication Critical patent/DE60042767D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00Disinfection, sterilisation or deodorisation of air
    • A61L9/015Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone
    • A61L9/04Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone using substances evaporated in the air without heating
    • A61L9/12Apparatus, e.g. holders, therefor
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00Disinfection, sterilisation or deodorisation of air
    • A61L9/01Deodorant compositions
    • A61L9/012Deodorant compositions characterised by being in a special form, e.g. gels, emulsions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE60042767T 1999-02-03 2000-02-01 Erhöhung der Spezifität von Nukleinsäureamplifizierungen durch Trägernukleinsäuren Expired - Lifetime DE60042767D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11849599P 1999-02-03 1999-02-03

Publications (1)

Publication Number Publication Date
DE60042767D1 true DE60042767D1 (de) 2009-10-01

Family

ID=22378957

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60042767T Expired - Lifetime DE60042767D1 (de) 1999-02-03 2000-02-01 Erhöhung der Spezifität von Nukleinsäureamplifizierungen durch Trägernukleinsäuren

Country Status (13)

Country Link
US (1) US6300075B1 (de)
EP (1) EP1026261B1 (de)
JP (2) JP2000279184A (de)
KR (1) KR100901392B1 (de)
CN (1) CN100379880C (de)
AT (1) ATE440147T1 (de)
AU (1) AU764914B2 (de)
CA (1) CA2295942C (de)
DE (1) DE60042767D1 (de)
DK (1) DK1026261T3 (de)
ES (1) ES2328443T3 (de)
NO (1) NO327267B1 (de)
PT (1) PT1026261E (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1422298B1 (de) * 1999-07-09 2010-01-27 Gen-Probe Incorporated HIV-1 Detektion mittels Nukleinsäureamplifizierung
AU2001290867A1 (en) 2000-09-14 2002-03-26 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
US6949368B2 (en) * 2001-01-30 2005-09-27 The Trustees Of Princeton University Compositions and methods for enhancing polynucleotide amplification reactions
EP1321532A1 (de) * 2001-12-19 2003-06-25 Boehringer Mannheim Gmbh Reagenz zur verbesserter PCR
US7772383B2 (en) * 2002-01-25 2010-08-10 The Trustees Of Princeton University Chemical PCR: Compositions for enhancing polynucleotide amplification reactions
EP1532275A4 (de) * 2002-07-26 2005-09-14 Applera Corp Biochemische mg-vermittelte warmstart-reaktionen
US20040235032A1 (en) * 2003-05-19 2004-11-25 Canon Kabushiki Kaisha PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method
DE602005011754D1 (de) * 2004-01-23 2009-01-29 Bio Merieux Inc Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren
EP1605060A1 (de) * 2004-06-10 2005-12-14 Biodynamics S.R.L. Verfahren und Reaktionsgemisch zur Steigerung der Spezifität und Genauigkeit von Polymerasereaktionen
US20070092899A1 (en) * 2005-10-21 2007-04-26 The Regents Of The University Of California Multiple displacement amplification with blocker DNA
US8206924B2 (en) * 2006-09-11 2012-06-26 Osaka University Trace mRNA amplification method and use thereof
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
WO2011046972A2 (en) * 2009-10-12 2011-04-21 Life Technologies Corporation Compositions and methods for suppressing primer interactions
KR101162422B1 (ko) 2009-11-30 2012-07-10 박수민 핵산 증폭용 조성물
WO2013115067A1 (ja) * 2012-01-31 2013-08-08 タカラバイオ株式会社 核酸合成反応の向上方法
UA117921C2 (uk) * 2012-10-22 2018-10-25 Байєр Кропсаєнс Нв Композиція, пристрій та спосіб ампліфікації нуклеїнових кислот

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8918226D0 (en) * 1989-08-09 1989-09-20 Wellcome Found Dna assays
US5646019A (en) * 1989-10-24 1997-07-08 Stratagene Method for producing primed nucleic acid templates
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5491086A (en) 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
JPH09168400A (ja) * 1995-12-20 1997-06-30 Sekisui Chem Co Ltd 核酸の定量方法
JP3082908B2 (ja) * 1996-07-12 2000-09-04 東洋紡績株式会社 リボ核酸の単離方法
AU2799400A (en) * 1999-01-29 2000-08-18 Bavarian Nordic Research Institute A/S Multiplex real-time pcr

Also Published As

Publication number Publication date
PT1026261E (pt) 2009-09-23
NO20000538L (no) 2000-08-04
EP1026261A3 (de) 2003-07-30
AU764914B2 (en) 2003-09-04
US6300075B1 (en) 2001-10-09
JP2011120594A (ja) 2011-06-23
NO327267B1 (no) 2009-05-25
KR20000076606A (ko) 2000-12-26
EP1026261B1 (de) 2009-08-19
ATE440147T1 (de) 2009-09-15
CA2295942C (en) 2009-01-27
CN100379880C (zh) 2008-04-09
AU1487700A (en) 2000-08-10
EP1026261A2 (de) 2000-08-09
CN1271019A (zh) 2000-10-25
CA2295942A1 (en) 2000-08-03
DK1026261T3 (da) 2009-11-02
JP2000279184A (ja) 2000-10-10
ES2328443T3 (es) 2009-11-13
NO20000538D0 (no) 2000-02-02
KR100901392B1 (ko) 2009-06-08

Similar Documents

Publication Publication Date Title
DE60042767D1 (de) Erhöhung der Spezifität von Nukleinsäureamplifizierungen durch Trägernukleinsäuren
EP1050587A3 (de) Erzeugung und Amplifizierung von Nukleinsäuren ausgehend von Ribonukleinsäuren
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
AU6846698A (en) Method of nucleic acid amplification
BR9801878B1 (pt) oligonucleotìdeos, método para amplificação de uma seqüência alvo de ácido nucléico e kit para realizar uma reação de amplificação de ácido nucléico.
EA200300991A1 (ru) Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
EA200100402A1 (ru) Способы амплификации и секвенирования нуклеиновых кислот
WO1997041260A3 (en) Subtractive amplification
ATE161291T1 (de) Verfahren und reagenzien zum nachweis von hepatitis c
ATE148925T1 (de) Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion
ATE361377T1 (de) Leistungstarke verknüpfung von nukleinsäuresegmenten
EP1715037A4 (de) Verfahren zur nukleinsäureamplifikation
ATE469243T1 (de) Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen
ATE203775T1 (de) Methode zur detektion einer gezielten nukleinsäure
DE60023480D1 (de) Amplifikation und sequenzierung von primer-paaren und deren verwendung
ATE497022T1 (de) Verfahren zur amplifikation von nukleinsäure
ATE455866T1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
WO2002020845A3 (en) Ultra yield amplification reaction
EP1548112A4 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus
DE50214183D1 (de) Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen
DE60331132D1 (de) Verfahren zur Quantifizierung von Nukleinsäuren
ATE347618T1 (de) Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
WO2001029248A3 (en) Method for amplifying and detecting nucleic acid

Legal Events

Date Code Title Description
8364 No opposition during term of opposition