WO2002020845A3 - Ultra yield amplification reaction - Google Patents

Ultra yield amplification reaction Download PDF

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Publication number
WO2002020845A3
WO2002020845A3 PCT/US2001/028028 US0128028W WO0220845A3 WO 2002020845 A3 WO2002020845 A3 WO 2002020845A3 US 0128028 W US0128028 W US 0128028W WO 0220845 A3 WO0220845 A3 WO 0220845A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
amplification reaction
reaction
primer
Prior art date
Application number
PCT/US2001/028028
Other languages
French (fr)
Other versions
WO2002020845A2 (en
Inventor
Linda Starr-Spires
Original Assignee
Univ Jefferson
Linda Starr-Spires
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Jefferson, Linda Starr-Spires filed Critical Univ Jefferson
Publication of WO2002020845A2 publication Critical patent/WO2002020845A2/en
Publication of WO2002020845A3 publication Critical patent/WO2002020845A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

The sensitivity, and therefore specificity, of the polymerase chain reaction is compromised by primer-dimer formation early in the amplification process. Described herein is a simple and novel technique to avoid the formation of primer-dimers. A target nucleic acid is first amplified in a 'pre-amplification' reaction, wherein an extremely low concentration of primers bind to the target nucleic acid and not to each other. This allows for the efficient use of the DNA polymerase, deoxynucleoside triphosphates and other reaction components, to extend and amplify the target nucleic acid.
PCT/US2001/028028 2000-09-08 2001-09-06 Ultra yield amplification reaction WO2002020845A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23126300P 2000-09-08 2000-09-08
US60/231,263 2000-09-08

Publications (2)

Publication Number Publication Date
WO2002020845A2 WO2002020845A2 (en) 2002-03-14
WO2002020845A3 true WO2002020845A3 (en) 2003-03-13

Family

ID=22868459

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/028028 WO2002020845A2 (en) 2000-09-08 2001-09-06 Ultra yield amplification reaction

Country Status (2)

Country Link
US (1) US20020031777A1 (en)
WO (1) WO2002020845A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7695941B2 (en) * 2005-06-16 2010-04-13 The United States Of America As Represented By The Secretary Of The Navy Multiplexed polymerase chain reaction for genetic sequence analysis
EP1666150B1 (en) 2004-11-20 2015-01-07 Roche Diagnostics GmbH Nucleic acid preparation
EP1658898A1 (en) * 2004-11-20 2006-05-24 Roche Diagnostics GmbH Nucleic acid preparation
DE102006003990A1 (en) * 2006-01-23 2007-08-02 Gebr. Schmid Gmbh & Co. Method and device for processing or processing silicon material
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2121956B1 (en) 2006-12-21 2016-08-17 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US7888858B2 (en) 2007-08-21 2011-02-15 Global Oled Technology Llc Light emitting diode device incorporating a white light emitting layer in combination with a plurality of optical microcavities
US9169512B2 (en) 2009-07-01 2015-10-27 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
KR101287431B1 (en) * 2010-05-07 2013-07-19 (주)진매트릭스 Primer composition for amplifying genetic region having various genetic variations in target genes, method for amplifying the target genes using the same, PCR amplification kit comprising the same and method for analyzing the genotype of the target genes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0469610A1 (en) * 1990-08-02 1992-02-05 SHIONOGI SEIYAKU KABUSHIKI KAISHA trading under the name of SHIONOGI & CO. LTD. Improved two-step PCR method
EP0866071A2 (en) * 1997-03-20 1998-09-23 F. Hoffmann-La Roche Ag Modified primers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0469610A1 (en) * 1990-08-02 1992-02-05 SHIONOGI SEIYAKU KABUSHIKI KAISHA trading under the name of SHIONOGI & CO. LTD. Improved two-step PCR method
EP0866071A2 (en) * 1997-03-20 1998-09-23 F. Hoffmann-La Roche Ag Modified primers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BROWNIE JANNINE ET AL: "The elimination of primer-dimer accumulation in PCR", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 16, 1997, pages 3235 - 3241, XP002152588, ISSN: 0305-1048 *
ERLICH H.A.: "PCR technology, Principles and Applications for DNA Amplification", 1989, STOCKTON PRESS, NEW YORK, US, XP002223319 *
GIBBS ET AL: "DIAGNOSIS OF NEW MUTATION DISEASES USING THE POLYMERASE CHAIN REACTION", PCR TECHNOLOGY. PRINCIPLES AND APPLICATIONS FOR DNA AMPLIFICATION, NEW YORK, STOCKTON PRESS, US, 1989, pages 171 - 190, XP000886474 *

Also Published As

Publication number Publication date
US20020031777A1 (en) 2002-03-14
WO2002020845A2 (en) 2002-03-14

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