WO2004016755A3 - Amplification of target nucleotide sequence without polymerase chain reaction - Google Patents

Amplification of target nucleotide sequence without polymerase chain reaction Download PDF

Info

Publication number
WO2004016755A3
WO2004016755A3 PCT/US2003/025544 US0325544W WO2004016755A3 WO 2004016755 A3 WO2004016755 A3 WO 2004016755A3 US 0325544 W US0325544 W US 0325544W WO 2004016755 A3 WO2004016755 A3 WO 2004016755A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
lcr
chain reaction
sequence
products
Prior art date
Application number
PCT/US2003/025544
Other languages
French (fr)
Other versions
WO2004016755A2 (en
Inventor
Tadashi Ryan Kawashima
Erik Holmlin
Donald M Crothers
Original Assignee
Geneohm Sciences
Tadashi Ryan Kawashima
Erik Holmlin
Donald M Crothers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geneohm Sciences, Tadashi Ryan Kawashima, Erik Holmlin, Donald M Crothers filed Critical Geneohm Sciences
Priority to AU2003258242A priority Critical patent/AU2003258242A1/en
Publication of WO2004016755A2 publication Critical patent/WO2004016755A2/en
Priority to US10/914,114 priority patent/US20050118616A1/en
Publication of WO2004016755A3 publication Critical patent/WO2004016755A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure relates to methods for generating single-stranded DNA molecules of defined sequence and length using ligase chain reaction (LCR). Specifically, a region of template containing target sequence is amplified by LCR, exogenous sequence is introduced by LCR primers or probes used in amplification, and LCR products may be used in further amplification steps involving rolling circle amplification (RCA) or polymerase chain reaction (PCR). LCR products may include sequence complementary to the backbone of a padlock probe, where the LCR product hybridizes to a padlock probe and after ligation of the padlock, serves as polymerazation primer. After amplification, single-stranded amplification products are trimmed to produce short single-stranded DNA molecules of defined sequence and length.
PCT/US2003/025544 2002-08-16 2003-08-14 Amplification of target nucleotide sequence without polymerase chain reaction WO2004016755A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003258242A AU2003258242A1 (en) 2002-08-16 2003-08-14 Amplification of target nucleotide sequence without polymerase chain reaction
US10/914,114 US20050118616A1 (en) 2002-08-16 2004-08-09 Amplification of target nucleotide sequence without polymerase chain reaction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40419502P 2002-08-16 2002-08-16
US60/404,195 2002-08-16

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/914,114 Continuation-In-Part US20050118616A1 (en) 2002-08-16 2004-08-09 Amplification of target nucleotide sequence without polymerase chain reaction

Publications (2)

Publication Number Publication Date
WO2004016755A2 WO2004016755A2 (en) 2004-02-26
WO2004016755A3 true WO2004016755A3 (en) 2004-08-26

Family

ID=31888341

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/025544 WO2004016755A2 (en) 2002-08-16 2003-08-14 Amplification of target nucleotide sequence without polymerase chain reaction

Country Status (2)

Country Link
AU (1) AU2003258242A1 (en)
WO (1) WO2004016755A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
GB201617491D0 (en) * 2016-10-14 2016-11-30 Moorlodge Biotech Ventures Limited Amplification of nucleic acids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6410278B1 (en) * 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
US6558928B1 (en) * 1998-03-25 2003-05-06 Ulf Landegren Rolling circle replication of padlock probes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558928B1 (en) * 1998-03-25 2003-05-06 Ulf Landegren Rolling circle replication of padlock probes
US6410278B1 (en) * 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid

Also Published As

Publication number Publication date
AU2003258242A8 (en) 2004-03-03
WO2004016755A2 (en) 2004-02-26
AU2003258242A1 (en) 2004-03-03

Similar Documents

Publication Publication Date Title
WO2003091406A3 (en) Amplification of dna to produce single-stranded product of defined sequence and length
WO2001079420A3 (en) Detection and amplification or rna using target-mediated ligation of dna by rna ligase
IL131966A0 (en) An in vitro method for construction of a dna library
EP1050587A3 (en) Generation and amplification of nucleic acids from ribonucleic acids
WO2004058987A3 (en) Nucleic acid amplification
JP5680078B2 (en) Nucleic acid normalized quantification method based on ligation
EP1350841A3 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
WO2004081225A3 (en) Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
WO2001020035A3 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2005089508A3 (en) Dna sequence detection by limited primer extension
WO2003027307A3 (en) Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
WO2004070053A3 (en) cDNA AMPLIFICATION FOR EXPRESSION PROFILING
WO2003102232A3 (en) Amplification of ribonucleic acids
NO20000538L (en) Enhancing the specificity of nucleic acid amplification by nucleic acid
DK1171634T3 (en) Amplification and sequencing primer pairs and their use
WO2003000927A3 (en) Method for the determination of telomere length
WO2004003173A3 (en) Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides
JP2008136451A (en) Method for amplifying nucleic acid
WO2004016755A3 (en) Amplification of target nucleotide sequence without polymerase chain reaction
EP1411117A4 (en) Oligonucleotides for detecting tubercle bacillus and method therefor
WO2005012499A3 (en) Methods for amplifying polymeric nucleic acids
RU2005104635A (en) METHOD FOR PROTECTING RT-PCR FROM CONTAMINATION BY AMPLIFICATION PRODUCTS BASED ON THE DESTRUCTIVE ACTION OF URAPYLES OF Glycosylase DNA
WO2001029248A3 (en) Method for amplifying and detecting nucleic acid
Park et al. A DNA Extraction Technique Using Sing le B uffer S ystem, A ccuP rep®-Q uick DNAK it
Wang et al. Methods for reducing non-specific priming in DNA detection

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 10914114

Country of ref document: US

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP