ATE469243T1 - Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen - Google Patents
Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturenInfo
- Publication number
- ATE469243T1 ATE469243T1 AT06017482T AT06017482T ATE469243T1 AT E469243 T1 ATE469243 T1 AT E469243T1 AT 06017482 T AT06017482 T AT 06017482T AT 06017482 T AT06017482 T AT 06017482T AT E469243 T1 ATE469243 T1 AT E469243T1
- Authority
- AT
- Austria
- Prior art keywords
- secondary structure
- sequence
- forming region
- primer
- destruction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US41226302P | 2002-09-20 | 2002-09-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE469243T1 true ATE469243T1 (de) | 2010-06-15 |
Family
ID=32326255
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06017482T ATE469243T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
AT03255861T ATE346169T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03255861T ATE346169T1 (de) | 2002-09-20 | 2003-09-18 | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen |
Country Status (6)
Country | Link |
---|---|
US (1) | US8394944B2 (de) |
EP (2) | EP1428892B1 (de) |
JP (1) | JP2004113240A (de) |
AT (2) | ATE469243T1 (de) |
DE (2) | DE60309817T2 (de) |
ES (1) | ES2276016T3 (de) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005084277A2 (en) * | 2004-02-27 | 2005-09-15 | University Of Denver | Method of isolating nucleic acid targets |
SE0401270D0 (sv) * | 2004-05-18 | 2004-05-18 | Fredrik Dahl | Method for amplifying specific nucleic acids in parallel |
US8628918B2 (en) | 2005-05-09 | 2014-01-14 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
US7803541B2 (en) | 2005-05-12 | 2010-09-28 | Panomics, Inc. | Multiplex branched-chain DNA assays |
CN101495650B (zh) | 2005-06-20 | 2015-02-04 | 领先细胞医疗诊断有限公司 | 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法 |
WO2007044427A2 (en) | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
WO2007058898A2 (en) | 2005-11-10 | 2007-05-24 | Panomics, Inc. | Detection of nucleic acids through amplification of surrogate nucleic acids |
ES2648564T3 (es) | 2010-10-21 | 2018-01-04 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
EP3286331B1 (de) * | 2015-04-24 | 2022-12-14 | Qiagen GmbH | Verfahren zur hybridisierung eines nukleinsäuremoleküls |
US11085073B2 (en) | 2015-04-24 | 2021-08-10 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on a solid support |
WO2016170179A1 (en) | 2015-04-24 | 2016-10-27 | Qiagen Gmbh | Method for immobilizing a nucleic acid molecule on solid support |
CN108368543B (zh) | 2015-10-12 | 2023-06-13 | 领先细胞医疗诊断有限公司 | 高噪声样品中原位检测核苷酸变体及相关的组合物和方法 |
US10415080B2 (en) | 2016-11-21 | 2019-09-17 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
US10844430B2 (en) * | 2018-01-24 | 2020-11-24 | Qiagen Sciences, Llc | DNA sequencing reaction additive |
US11549139B2 (en) | 2018-05-14 | 2023-01-10 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
WO2023248310A1 (ja) * | 2022-06-20 | 2023-12-28 | 株式会社日立ハイテク | プライマー、dna検出方法およびdna検出キット |
Family Cites Families (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU602875B2 (en) | 1985-12-18 | 1990-11-01 | British Technology Group Limited | Newcastle disease virus gene clones |
SE454781B (sv) | 1986-10-17 | 1988-05-30 | Wallac Oy | Hybridiseringsforfarande for detektion av polynukleotidsekvens |
US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
US5030557A (en) * | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
US5200314A (en) | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
EP0463395B1 (de) | 1990-06-22 | 1997-05-14 | F. Hoffmann-La Roche Ag | Nachweis von schwachen Metabolisierungsreagentien von Drogen |
US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
ES2082256T3 (es) * | 1992-03-23 | 1996-03-16 | Hoffmann La Roche | Metodo de deteccion del adn. |
US5827648A (en) | 1995-12-13 | 1998-10-27 | Chiron Corporation | Differential hybridization for relative quantification of variant populations |
US6187538B1 (en) | 1993-12-13 | 2001-02-13 | Bayer Corporation | Differential hybridization for relative quantification of variant populations |
EP0754241B1 (de) | 1994-04-04 | 1998-12-02 | Ciba Corning Diagnostics Corp. | Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen |
US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
US5622822A (en) | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
US5747248A (en) | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
US5981180A (en) | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
ES2262902T3 (es) | 1995-12-05 | 2006-12-01 | Jorn Erland Koch | Una reaccion en cascada de amplificacion de acido nucleico. |
US6139800A (en) | 1997-06-23 | 2000-10-31 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
AU9220498A (en) | 1997-09-04 | 1999-03-22 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof |
US6136568A (en) | 1997-09-15 | 2000-10-24 | Hiatt; Andrew C. | De novo polynucleotide synthesis using rolling templates |
US6054568A (en) * | 1998-01-16 | 2000-04-25 | The Perkin-Elmer Corporation | Nucleobase oligomers |
KR100593712B1 (ko) | 1998-01-22 | 2006-06-30 | 루미넥스 코포레이션 | 다수의 형광 시그널을 갖는 마이크로입자 |
CA2319170A1 (en) | 1998-01-27 | 1999-07-29 | Clinical Micro Sensors, Inc. | Amplification of nucleic acids with electronic detection |
JP4196236B2 (ja) | 1998-03-17 | 2008-12-17 | 東洋紡績株式会社 | 核酸増幅用試薬および配列特異的な核酸増幅法 |
CA2331896C (en) | 1998-05-14 | 2009-09-15 | Luminex Corporation | Diode laser based measurement apparatus |
US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
US6322980B1 (en) | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
US6287778B1 (en) | 1999-10-19 | 2001-09-11 | Affymetrix, Inc. | Allele detection using primer extension with sequence-coded identity tags |
US20020028455A1 (en) * | 2000-05-03 | 2002-03-07 | Laibinis Paul E. | Methods and reagents for assembling molecules on solid supports |
US6214557B1 (en) | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
EP1412526A2 (de) | 2001-06-29 | 2004-04-28 | Syngenta Participations AG | Verfahren zur verbesserten detektion und unterschiedung von differenzieller genexpression mittels enzymatischer ligation von proben und amplifizierung |
-
2003
- 2003-09-15 US US10/667,191 patent/US8394944B2/en active Active
- 2003-09-18 EP EP03255861A patent/EP1428892B1/de not_active Expired - Lifetime
- 2003-09-18 EP EP06017482A patent/EP1785491B1/de not_active Expired - Lifetime
- 2003-09-18 DE DE60309817T patent/DE60309817T2/de not_active Expired - Lifetime
- 2003-09-18 AT AT06017482T patent/ATE469243T1/de not_active IP Right Cessation
- 2003-09-18 DE DE60332775T patent/DE60332775D1/de not_active Expired - Lifetime
- 2003-09-18 ES ES03255861T patent/ES2276016T3/es not_active Expired - Lifetime
- 2003-09-18 AT AT03255861T patent/ATE346169T1/de not_active IP Right Cessation
- 2003-09-19 JP JP2003329188A patent/JP2004113240A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP1785491B1 (de) | 2010-05-26 |
EP1428892B1 (de) | 2006-11-22 |
DE60332775D1 (de) | 2010-07-08 |
DE60309817D1 (de) | 2007-01-04 |
ES2276016T3 (es) | 2007-06-16 |
JP2004113240A (ja) | 2004-04-15 |
ATE346169T1 (de) | 2006-12-15 |
US8394944B2 (en) | 2013-03-12 |
DE60309817T2 (de) | 2007-09-13 |
EP1785491A1 (de) | 2007-05-16 |
EP1428892A1 (de) | 2004-06-16 |
US20040132061A1 (en) | 2004-07-08 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE469243T1 (de) | Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen | |
WO2007127992A3 (en) | Use of base-modified deoxynucleoside triphosphates | |
ATE394410T1 (de) | Hochspezifische primer, amplifizierungsverfahren und kits | |
WO2002052031A3 (en) | Nucleic acid amplification | |
WO2005089508A3 (en) | Dna sequence detection by limited primer extension | |
WO2009135093A3 (en) | Rnase-h-based assays utilizing modified rna monomers | |
RU2014144722A (ru) | Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце | |
DE60108897D1 (de) | Methoden für externe kontrollen zur nukleinsäure amplifizierung | |
WO2005091971A3 (en) | Compositions for use in identification of alphaviruses | |
EP1050587A3 (de) | Erzeugung und Amplifizierung von Nukleinsäuren ausgehend von Ribonukleinsäuren | |
WO2008033442A3 (en) | Methods and compositions for performing low background multiplex nucleic acid amplification reactions | |
WO2004013346A8 (en) | Methods and compositions for genotyping | |
US9121046B2 (en) | Reducing template independent primer extension and threshold time for loop mediated isothermal amplification | |
WO2005003386A3 (en) | Oligonucleotide ligation assay by detecting released pyrophosphate | |
WO2001092569A3 (en) | Formulation for polymerase chain reaction and vessel containing same | |
KR930004477A (ko) | 미코박테리아 프라이머 및 탐침 | |
DK1026261T3 (da) | Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre | |
EP1736543A4 (de) | Verfahren zum nachweis und zur quantifizierung von endogener weizen-dna-sequenz | |
KR20120020067A (ko) | 헤파티티스 c 바이러스 검출용 키트 및 이를 이용한 헤파티티스 c 바이러스의 검출 방법 | |
WO2008108843A3 (en) | Methods and kits for amplifying dna | |
CN101886131B (zh) | 用两性离子化合物提高核酸杂交特异性的方法 | |
ATE497022T1 (de) | Verfahren zur amplifikation von nukleinsäure | |
JP2006521824A5 (de) | ||
EP1862558A4 (de) | Gennachweisverfahren | |
WO2006039564A3 (en) | Assay for detecting and quantifying hiv-1 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |