UA117921C2 - Композиція, пристрій та спосіб ампліфікації нуклеїнових кислот - Google Patents

Композиція, пристрій та спосіб ампліфікації нуклеїнових кислот

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Publication number
UA117921C2
UA117921C2 UAA201505034A UAA201505034A UA117921C2 UA 117921 C2 UA117921 C2 UA 117921C2 UA A201505034 A UAA201505034 A UA A201505034A UA A201505034 A UAA201505034 A UA A201505034A UA 117921 C2 UA117921 C2 UA 117921C2
Authority
UA
Ukraine
Prior art keywords
amplification
dna
nucleic acid
mixture
compositions
Prior art date
Application number
UAA201505034A
Other languages
English (en)
Inventor
Піт Соетаерт
Мартін Лойбе
Бастіан Будде
Клаус ОХМАНН
Міхаель Харнау
Original Assignee
Байєр Кропсаєнс Нв
Байєр Кропсаєнс Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Байєр Кропсаєнс Нв, Байєр Кропсаєнс Аг filed Critical Байєр Кропсаєнс Нв
Publication of UA117921C2 publication Critical patent/UA117921C2/uk

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Винахід належить до композиції, яка містить суміш дезоксинуклеотидів (dNTPs), двовалентних катіонів, ферменту, здатного ампліфікувати ДНК, та праймерів, придатних для ампліфікації цільової нуклеїнової кислоти або цільової ДНК, де вказана суміш має буферну ємність з достатньо низьким рівнем іонної сили для того, щоб достатньо нейтралізувати лужний розчин, що містить матричну нуклеїнову кислоту або ДНК, що дозволить ферменту ампліфікації функціонувати, при цьому вказана суміш містить сіль, яка містить амоній в якості катіону та аніон слабкої кислоти, в якій вказаною сіллю є амонію пентаборат, та дикарбонову кислоту. Винахід належить також до набору, пристрою та способу ампліфікації цільової нуклеїнової кислоти або цільової ДНК.
UAA201505034A 2012-10-22 2013-10-18 Композиція, пристрій та спосіб ампліфікації нуклеїнових кислот UA117921C2 (uk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12189381 2012-10-22
PCT/EP2013/071830 WO2014064002A1 (en) 2012-10-22 2013-10-18 Methods, compositions and devices for amplification of nucleic acids

Publications (1)

Publication Number Publication Date
UA117921C2 true UA117921C2 (uk) 2018-10-25

Family

ID=47046465

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201505034A UA117921C2 (uk) 2012-10-22 2013-10-18 Композиція, пристрій та спосіб ампліфікації нуклеїнових кислот

Country Status (10)

Country Link
US (1) US10087491B2 (uk)
EP (1) EP2909338B1 (uk)
AR (1) AR093086A1 (uk)
BR (1) BR112015008268A2 (uk)
CA (1) CA2888720A1 (uk)
CO (1) CO7400880A2 (uk)
RU (1) RU2666988C2 (uk)
UA (1) UA117921C2 (uk)
UY (1) UY35093A (uk)
WO (1) WO2014064002A1 (uk)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113943773A (zh) 2014-11-05 2022-01-18 加州理工学院 生物体对药物的响应的微流体测量
US11168347B2 (en) 2016-09-23 2021-11-09 California Institute Of Technology Digital quantification of DNA replication and/or chromosome segregation based determination of antimicrobial susceptibility
WO2018089943A1 (en) * 2016-11-10 2018-05-17 Slipchip Corporation Probe detection of loop-mediated amplification products
WO2019075264A1 (en) 2017-10-11 2019-04-18 California Institute Of Technology ANTIBIOSENSITIVITY OF MICROORGANISMS AND COMPOSITIONS, METHODS AND ASSOCIATED SYSTEMS
US10450616B1 (en) 2018-05-09 2019-10-22 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Chlamydia trachomatis
JP7329912B2 (ja) * 2018-10-09 2023-08-21 公益財団法人筑波メディカルセンター 微生物の検出方法
US10954572B2 (en) 2019-07-25 2021-03-23 Talis Biomedical Corporation Polynucleotides for the amplification and detection of Neisseria gonorrhoeae
US11891662B2 (en) 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
CN112813150A (zh) * 2021-03-04 2021-05-18 重庆大学 谷子叶片直接pcr扩增的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6300075B1 (en) 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
EP1390466A4 (en) * 2001-04-26 2010-07-28 Pressure Biosciences Inc MULTI-CHAMBER DEVICE AND ITS USE FOR PROCESSING BIOLOGICAL SAMPLES
WO2003042383A1 (fr) * 2001-11-14 2003-05-22 Toyo Boseki Kabushiki Kaisha Promoteurs de replication de l'adn, facteurs associes a l'adn polymerase et utilisation de ces derniers
WO2003048391A1 (en) 2001-12-04 2003-06-12 Bio3 Research Srl Double buffer system for polymerase chain reaction buffers
ES2373686T3 (es) 2005-06-23 2012-02-07 Biocartis Sa Cartucho, sistema y procedimiento para diagnósticos médicos automatizados.
US8921073B2 (en) * 2006-06-23 2014-12-30 Illumina, Inc. Devices and systems for creation of DNA cluster arrays
GB0618966D0 (en) 2006-09-26 2006-11-08 Iti Scotland Ltd Cartridge system
EP2077337A1 (en) 2007-12-26 2009-07-08 Eppendorf Array Technologies SA Amplification and detection composition, method and kit
JP5572943B2 (ja) * 2008-12-17 2014-08-20 株式会社島津製作所 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット

Also Published As

Publication number Publication date
EP2909338A1 (en) 2015-08-26
RU2015119238A (ru) 2016-12-10
BR112015008268A2 (pt) 2017-11-28
US20150267266A1 (en) 2015-09-24
UY35093A (es) 2014-05-30
AR093086A1 (es) 2015-05-20
CA2888720A1 (en) 2014-05-01
EP2909338B1 (en) 2018-08-15
RU2666988C2 (ru) 2018-09-13
CO7400880A2 (es) 2015-09-30
US10087491B2 (en) 2018-10-02
WO2014064002A1 (en) 2014-05-01

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