KR900018376A - 리보핵산(rna)의 합성방법 - Google Patents

리보핵산(rna)의 합성방법 Download PDF

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Publication number
KR900018376A
KR900018376A KR1019900006503A KR900006503A KR900018376A KR 900018376 A KR900018376 A KR 900018376A KR 1019900006503 A KR1019900006503 A KR 1019900006503A KR 900006503 A KR900006503 A KR 900006503A KR 900018376 A KR900018376 A KR 900018376A
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South Korea
Prior art keywords
rna
sequence
dna
stranded dna
target sequence
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KR1019900006503A
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KR0185376B1 (ko
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데이비 체릴
티 말레크 로렌스
프랭클린 렌즈 피터
비이라드 프리츠
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에프.쥐. 엠 헤로만스 .에이. 쥐. 제이 베어미렌
악조 엔. 브이
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Publication of KR900018376A publication Critical patent/KR900018376A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster

Abstract

내용 없음

Description

리보핵산(RNA)의 합성방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (3)

  1. 타겟 서열이 존재하는 데옥시리보 핵산(DNA)으로 부터 시작하여 리보핵산(RNA)를 합성하는 방법으로서, 하나 이상의 제한효소로 상기 DNA를 처리한후, 수득한 DNA단편물을 분리하고, 그에 의해 단일가닥 DNA가 형성되며, 그후 타겟 서열의 일부에 대응하는 뉴클레오티드 서열에 결합하는 프로모터 서열을 함유한 하나의 핵산 프라이머를 적당한 조건하에 대응하는 단일가닥 DNA에 하이브리드 시키며, 그후, 하이브리드 형태로 수득한 2개의 핵산 서열을 DNA폴리머라제에 의해 3'-말단으로 부터 연장시켜서 이중가닥 DNA를 생성하며, 이것을 RNA합성을 위해 RNA폴리머라제에 의해 매트릭스로 사용하는 것을 특징으로 하는 방법.
  2. 제1항에 있어서, 합성된 RNA를 변성 조건하에 겔 전기영동과 블롯팅으로 검출한후 라벨된 특이 올리고뉴클레오티드 서열과 하이브리드 반응을 시키는 것을 특징으로 하는 방법.
  3. 제1항의 RNA합성을 위한 시험키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900006503A 1989-05-10 1990-05-09 리보핵산의 합성방법 KR0185376B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL89.01172 1989-05-10
NL8901172 1989-05-10
NL8901172 1989-05-10

Publications (2)

Publication Number Publication Date
KR900018376A true KR900018376A (ko) 1990-12-21
KR0185376B1 KR0185376B1 (ko) 1999-04-01

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KR1019900006503A KR0185376B1 (ko) 1989-05-10 1990-05-09 리보핵산의 합성방법

Country Status (13)

Country Link
US (1) US5466586A (ko)
EP (1) EP0397269B1 (ko)
JP (1) JP2839639B2 (ko)
KR (1) KR0185376B1 (ko)
AT (1) ATE118549T1 (ko)
AU (1) AU641159B2 (ko)
CA (1) CA2016358C (ko)
DE (1) DE69016826T2 (ko)
DK (1) DK0397269T3 (ko)
ES (1) ES2070995T3 (ko)
FI (1) FI102387B (ko)
IE (1) IE66597B1 (ko)
ZA (1) ZA903453B (ko)

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Also Published As

Publication number Publication date
IE901624L (en) 1990-11-10
EP0397269A1 (en) 1990-11-14
CA2016358C (en) 2000-11-28
CA2016358A1 (en) 1990-11-10
KR0185376B1 (ko) 1999-04-01
FI102387B1 (fi) 1998-11-30
ATE118549T1 (de) 1995-03-15
JP2839639B2 (ja) 1998-12-16
AU5487790A (en) 1990-11-15
US5466586A (en) 1995-11-14
ZA903453B (en) 1991-02-27
DE69016826D1 (de) 1995-03-23
DE69016826T2 (de) 1995-06-08
FI902318A0 (fi) 1990-05-09
EP0397269B1 (en) 1995-02-15
FI102387B (fi) 1998-11-30
DK0397269T3 (da) 1995-05-29
ES2070995T3 (es) 1995-06-16
JPH03180184A (ja) 1991-08-06
AU641159B2 (en) 1993-09-16
IE66597B1 (en) 1996-01-24

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