DE69016826T2 - Verfahren zur Synthese von Ribonukleinsäure (RNS). - Google Patents

Verfahren zur Synthese von Ribonukleinsäure (RNS).

Info

Publication number
DE69016826T2
DE69016826T2 DE69016826T DE69016826T DE69016826T2 DE 69016826 T2 DE69016826 T2 DE 69016826T2 DE 69016826 T DE69016826 T DE 69016826T DE 69016826 T DE69016826 T DE 69016826T DE 69016826 T2 DE69016826 T2 DE 69016826T2
Authority
DE
Germany
Prior art keywords
synthesis
ribonucleic acid
rna
target sequence
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69016826T
Other languages
English (en)
Other versions
DE69016826D1 (de
Inventor
Lawrence T Malek
Cheryl Davey
Peter Franklin Lens
Frits Wielaard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo NV
Original Assignee
Akzo NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo NV filed Critical Akzo NV
Publication of DE69016826D1 publication Critical patent/DE69016826D1/de
Application granted granted Critical
Publication of DE69016826T2 publication Critical patent/DE69016826T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/705Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
DE69016826T 1989-05-10 1990-05-08 Verfahren zur Synthese von Ribonukleinsäure (RNS). Expired - Lifetime DE69016826T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8901172 1989-05-10

Publications (2)

Publication Number Publication Date
DE69016826D1 DE69016826D1 (de) 1995-03-23
DE69016826T2 true DE69016826T2 (de) 1995-06-08

Family

ID=19854626

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69016826T Expired - Lifetime DE69016826T2 (de) 1989-05-10 1990-05-08 Verfahren zur Synthese von Ribonukleinsäure (RNS).

Country Status (13)

Country Link
US (1) US5466586A (de)
EP (1) EP0397269B1 (de)
JP (1) JP2839639B2 (de)
KR (1) KR0185376B1 (de)
AT (1) ATE118549T1 (de)
AU (1) AU641159B2 (de)
CA (1) CA2016358C (de)
DE (1) DE69016826T2 (de)
DK (1) DK0397269T3 (de)
ES (1) ES2070995T3 (de)
FI (1) FI102387B (de)
IE (1) IE66597B1 (de)
ZA (1) ZA903453B (de)

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US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
KR100289793B1 (ko) * 1992-01-29 2001-12-01 테츠오 토미나가 폴리누클레오티드고정지지체
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
JPH07509361A (ja) * 1992-07-28 1995-10-19 日立化成工業株式会社 遺伝子検出システム
US5712127A (en) * 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
DE19633427C2 (de) * 1996-08-20 2001-10-04 Hubert S Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz
DE19812103A1 (de) * 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
CN1269966C (zh) * 2001-03-07 2006-08-16 拜奥默里克斯有限公司 用基于转录的扩增来扩增和检测hbvdna的方法
BR0207914A (pt) * 2001-03-07 2005-08-16 Biomerieux Bv Método para a amplificação baseada em transcrição de uma sequência de ácido nucleico alvo partindo de dna opcionalmente presente em uma amostra
US8137911B2 (en) * 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
WO2004059289A2 (en) * 2001-05-22 2004-07-15 Epicentre Technologies Target-dependent transcription using deletion mutants of n4 rna polymerase
US7504215B2 (en) 2002-07-12 2009-03-17 Affymetrix, Inc. Nucleic acid labeling methods
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
AU2003297557B2 (en) 2002-11-21 2009-02-26 Cellscript, Inc. Methods for using primers that encode one strand of a double-stranded promoter
US7266295B2 (en) * 2003-04-17 2007-09-04 Intel Corporation Modular reconfigurable multi-server system and method for high-speed networking within photonic burst-switched network
EP2071031B1 (de) * 2004-08-27 2013-10-09 Gen-Probe Incorporated Einfach-Primernukleinsäuren-Erweiterungsverfahren
US7713697B2 (en) * 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
US8202727B2 (en) 2005-05-17 2012-06-19 Ozgene Pty Ltd. Sequential cloning system
US8293684B2 (en) * 2006-11-29 2012-10-23 Exiqon Locked nucleic acid reagents for labelling nucleic acids
US8183359B2 (en) * 2007-03-01 2012-05-22 Gen-Probe Incorporated Kits for amplifying DNA
EP2173872B1 (de) 2007-06-29 2014-04-02 CellScript, Inc. Copy-dna und sense-rna
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
WO2010148085A1 (en) 2009-06-16 2010-12-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Rna nanoparticles and methods of use
TWI518325B (zh) 2010-02-04 2016-01-21 自治醫科大學 對alk抑制劑具有先抗性或後抗性癌症的識別、判斷和治療
EP3578205A1 (de) 2010-08-06 2019-12-11 ModernaTX, Inc. Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung
RU2013120302A (ru) 2010-10-01 2014-11-20 Модерна Терапьютикс, Инк. Сконструированные нуклеиновые кислоты и способы их применения
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US9428535B2 (en) 2011-10-03 2016-08-30 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
LT2791160T (lt) 2011-12-16 2022-06-10 Modernatx, Inc. Modifikuotos mrnr sudėtys
FR2984357B1 (fr) 2011-12-16 2016-11-18 Biomerieux Sa Procede d'amplification transcriptionnelle d'acides nucleiques reunissant des etapes de temperatures differentes
US9192651B2 (en) 2012-04-02 2015-11-24 Moderna Therapeutics, Inc. Modified polynucleotides for the production of secreted proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
WO2014081507A1 (en) 2012-11-26 2014-05-30 Moderna Therapeutics, Inc. Terminally modified rna
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2015006344A1 (en) * 2013-07-08 2015-01-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for synthesizing selectively labeled rna
EP3842542A1 (de) 2013-08-28 2021-06-30 Becton, Dickinson and Company Massiv parallele einzelzellanalyse
US10301621B2 (en) 2013-09-17 2019-05-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Multifunctional RNA nanoparticles and methods of use
EP3052106A4 (de) 2013-09-30 2017-07-19 ModernaTX, Inc. Polynukleotide zur codierung immunmodulierender polypeptide
CA2926218A1 (en) 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
US20160274095A1 (en) 2013-11-13 2016-09-22 Inserm (Institut National De La Sante Et De La Recherche Medicale Kits-of-Parts Comprising Nucleic Acids Able to Form a Kissing Complex and Their uses Thereof
US10517890B2 (en) 2014-05-06 2019-12-31 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Triggering RNA interference with RNA-DNA and DNA-RNA nanoparticles
US11512313B2 (en) 2017-04-03 2022-11-29 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Functionally-interdependent shape switching nucleic acid nanoparticles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4766072A (en) * 1985-07-17 1988-08-23 Promega Corporation Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
ATE92538T1 (de) * 1988-01-21 1993-08-15 Genentech Inc Verstaerkung und nachweis von nukleinsaeuresequenzen.
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process

Also Published As

Publication number Publication date
EP0397269A1 (de) 1990-11-14
IE901624L (en) 1990-11-10
ZA903453B (en) 1991-02-27
DE69016826D1 (de) 1995-03-23
ES2070995T3 (es) 1995-06-16
US5466586A (en) 1995-11-14
CA2016358A1 (en) 1990-11-10
JP2839639B2 (ja) 1998-12-16
ATE118549T1 (de) 1995-03-15
AU641159B2 (en) 1993-09-16
KR0185376B1 (ko) 1999-04-01
FI102387B1 (fi) 1998-11-30
KR900018376A (ko) 1990-12-21
CA2016358C (en) 2000-11-28
JPH03180184A (ja) 1991-08-06
FI102387B (fi) 1998-11-30
FI902318A0 (fi) 1990-05-09
DK0397269T3 (da) 1995-05-29
IE66597B1 (en) 1996-01-24
EP0397269B1 (de) 1995-02-15
AU5487790A (en) 1990-11-15

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