CA2264967A1 - Method of dna sequencing - Google Patents

Method of dna sequencing

Info

Publication number
CA2264967A1
CA2264967A1 CA002264967A CA2264967A CA2264967A1 CA 2264967 A1 CA2264967 A1 CA 2264967A1 CA 002264967 A CA002264967 A CA 002264967A CA 2264967 A CA2264967 A CA 2264967A CA 2264967 A1 CA2264967 A1 CA 2264967A1
Authority
CA
Canada
Prior art keywords
nucleic acid
transcription product
methods
rna polymerase
determining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002264967A
Other languages
French (fr)
Other versions
CA2264967C (en
Inventor
Yoshihide Hayashizaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon GeneTech Co Ltd
RIKEN Institute of Physical and Chemical Research
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2264967A1 publication Critical patent/CA2264967A1/en
Application granted granted Critical
Publication of CA2264967C publication Critical patent/CA2264967C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed are methods for determining DNA nucleotide sequences comprising reacting ribonucleoside 5'-triphosphates and 3'-dNTP derivatives in the presence of an RNA polymerase modified so as to enhance its ability for incorporating the 3'-dNTP derivatives and a DNA fragment containing a promoter sequence for the RNA polymerase to obtain a nucleic acid transcription product, separating the resulting nucleic acid transcription product, and determining a nucleic acid sequence from the resulting separated fraction. These methods can produce a transcription product of a long chain and afford more accurate sequence data where fluctuation of signals from labeled deoxyribonucleotides is reduced.
CA002264967A 1997-07-07 1998-07-06 Method of dna sequencing Expired - Fee Related CA2264967C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP19647897 1997-07-07
JP9/196478 1997-07-07
JP10/155847 1998-06-04
JP15584798A JP3318579B2 (en) 1997-07-07 1998-06-04 DNA sequencing method
PCT/JP1998/003039 WO1999002729A1 (en) 1997-07-07 1998-07-06 Method of dna sequencing

Publications (2)

Publication Number Publication Date
CA2264967A1 true CA2264967A1 (en) 1999-01-21
CA2264967C CA2264967C (en) 2008-09-23

Family

ID=26483751

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002264967A Expired - Fee Related CA2264967C (en) 1997-07-07 1998-07-06 Method of dna sequencing

Country Status (7)

Country Link
US (1) US6365350B1 (en)
EP (1) EP0978569B1 (en)
JP (1) JP3318579B2 (en)
KR (1) KR100551344B1 (en)
CA (1) CA2264967C (en)
DE (1) DE69826458T2 (en)
WO (1) WO1999002729A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3489991B2 (en) * 1997-07-07 2004-01-26 理化学研究所 3'-deoxyribonucleotide derivative
JP3172710B2 (en) * 1997-07-07 2001-06-04 理化学研究所 RNA polymerase
JP2002360256A (en) * 2001-06-04 2002-12-17 Inst Of Physical & Chemical Res Method for producing rna probe, method for detecting target nucleic acid, and kit for producing rna probe
JP2003061684A (en) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Method for dna base sequence determination
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US20060084799A1 (en) * 2003-09-24 2006-04-20 Williams Lewis T Human cDNA clones comprising polynucleotides encoding polypeptides and methods of their use
US20050075543A1 (en) * 2003-10-03 2005-04-07 Calabrese Charles A. Method of anonymous medical testing and providing the patient with the test results
US20070274988A1 (en) * 2003-10-10 2007-11-29 Five Prime Therapeautics, Inc. Kiaa0779, Splice Variants Thereof, and Methods of Their Use
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
JP4621921B2 (en) * 2005-07-27 2011-02-02 国立大学法人群馬大学 Novel nucleic acid derivative and method for producing polynucleotide using the same
US20080206850A1 (en) * 2007-02-28 2008-08-28 Dellinger Douglas J Methods and compositions for DNA synthesis
DK3133080T3 (en) * 2008-01-18 2018-11-26 Inst Of Organic Chemistry And Biochemistry Of The Academy Of Sciences Of The Czech Republic PRESENT UNKNOWN CYTOSTATIC 7-DEAZAPURIN NUCLEOSIDES
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
EP4355757A1 (en) * 2021-06-17 2024-04-24 F. Hoffmann-La Roche AG Nucleoside-5 -oligophosphates having a cationically-modified nucleobase

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6061594A (en) 1983-09-05 1985-04-09 ジヨエル　ブレスラ Fixed rna
FI87360C (en) 1990-01-18 1992-12-28 Neste Oy Process for preparing ethylene-propylene copolymers in particulate form
US6027877A (en) 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
JPH07327698A (en) 1994-04-13 1995-12-19 S R L:Kk Method for detecting variation of dna
GB9408344D0 (en) 1994-04-27 1994-06-15 St James S University Hospital Nucleic acid assays
ES2072238T3 (en) 1994-10-17 1997-01-16 Harvard College DNA-POLYMERASES THAT HAVE A UNION SITE WITH MODIFIED NUCLEOTIDES FOR DNA SEQUENCING.
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
CA2204378C (en) * 1994-11-07 2007-05-08 Yoshihide Hayashizaki Method for determining dna nucleotide sequence
SE9602062D0 (en) 1996-05-29 1996-05-29 Pharmacia Biotech Ab Method for detection of mutations

Also Published As

Publication number Publication date
WO1999002729A1 (en) 1999-01-21
DE69826458D1 (en) 2004-10-28
JPH1175898A (en) 1999-03-23
KR20000068502A (en) 2000-11-25
JP3318579B2 (en) 2002-08-26
US6365350B1 (en) 2002-04-02
DE69826458T2 (en) 2006-02-23
KR100551344B1 (en) 2006-02-09
EP0978569B1 (en) 2004-09-22
CA2264967C (en) 2008-09-23
EP0978569A4 (en) 2002-09-04
EP0978569A1 (en) 2000-02-09

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