EP0210021A3 - Carbonic anhydrase inhibitor-tagged nucleic acid probes - Google Patents

Carbonic anhydrase inhibitor-tagged nucleic acid probes Download PDF

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Publication number
EP0210021A3
EP0210021A3 EP86305300A EP86305300A EP0210021A3 EP 0210021 A3 EP0210021 A3 EP 0210021A3 EP 86305300 A EP86305300 A EP 86305300A EP 86305300 A EP86305300 A EP 86305300A EP 0210021 A3 EP0210021 A3 EP 0210021A3
Authority
EP
European Patent Office
Prior art keywords
carbonic anhydrase
nucleic acid
methods
probes
reporter group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP86305300A
Other languages
German (de)
French (fr)
Other versions
EP0210021A2 (en
Inventor
Emil Thomas Kaiser
Gary Fred Musso
Soumitra Shankar Ghosh
Leslie Eleazer Orgel
Geoffrey Myles Wahl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siska Diagnostics Inc
Original Assignee
Siska Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siska Diagnostics Inc filed Critical Siska Diagnostics Inc
Publication of EP0210021A2 publication Critical patent/EP0210021A2/en
Publication of EP0210021A3 publication Critical patent/EP0210021A3/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic Table
    • C07F7/02—Silicon compounds
    • C07F7/08—Compounds having one or more C—Si linkages
    • C07F7/18—Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
    • C07F7/1804—Compounds having Si-O-C linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Nucleic acid hybridization probes are provided which comprise nucleoside bases or terminal nucleotide phosph­ ates chemically linked to aromatic sulfonamide inhibitors of carbonic anhydrase. Methods of preparing probes of the in­ vention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. A probe of the invention is detected by binding to it a reporter group, such as a homopolymer or hetero­ polymer of enzymes, which includes a carbonic anhydrase which binds to the inhibitor linked to the probe, and then det­ ecting the bound reporter group, as by production of a fluor­ escent or colored product in a reaction catalyzed by an en­ zyme component of the reporter group. Also provided are en­ zyme immunoassays wherein detection of antibody is by a process which comprises a chemical reaction catalyzed by a carbonic anhydrase.
EP86305300A 1985-07-09 1986-07-09 Carbonic anhydrase inhibitor-tagged nucleic acid probes Withdrawn EP0210021A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/753,176 US4780405A (en) 1985-06-25 1985-07-09 Carbonic anhydrase inhibitor-tagged nucleic acid probes
US753176 2004-01-06

Publications (2)

Publication Number Publication Date
EP0210021A2 EP0210021A2 (en) 1987-01-28
EP0210021A3 true EP0210021A3 (en) 1987-09-02

Family

ID=25029490

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86305300A Withdrawn EP0210021A3 (en) 1985-07-09 1986-07-09 Carbonic anhydrase inhibitor-tagged nucleic acid probes

Country Status (4)

Country Link
US (1) US4780405A (en)
EP (1) EP0210021A3 (en)
JP (1) JPS6214800A (en)
CA (1) CA1294899C (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US5210203A (en) * 1985-06-25 1993-05-11 Siska Diagnostics, Inc. Sulfonamide-derivative compounds for tagging nucleic acid probes
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
GB2209754A (en) * 1987-09-17 1989-05-24 Thomas Lee Mattson Polyaldehydic polynucleotides in use as probes,their preparation and use
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6277569B1 (en) 1990-09-20 2001-08-21 Vysis, Inc. Methods for multiple direct label probe detection of multiple chromosomes or regions thereof by in situ hybridization
US5491224A (en) * 1990-09-20 1996-02-13 Bittner; Michael L. Direct label transaminated DNA probe compositions for chromosome identification and methods for their manufacture
US20060078914A1 (en) * 1993-01-05 2006-04-13 Gelfand David H Homogeneous assay system
JPH0737397Y2 (en) * 1993-08-03 1995-08-23 コルチトーン補聴器株式会社 Hearing aid telephone listening device
US6048688A (en) * 1996-11-12 2000-04-11 Kimberly-Clark Corporation Method for detection of Pseudomonas aeruginosa using polymerase chain reaction
US6956695B2 (en) * 2001-03-19 2005-10-18 Ikonisys, Inc. System and method for increasing the contrast of an image produced by an epifluorescence microscope
US8709721B2 (en) 2009-10-01 2014-04-29 Riken Fluorescent molecule and method for detecting target nucleic acid
WO2018218250A2 (en) * 2017-05-26 2018-11-29 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
CN107417681B (en) * 2017-06-15 2020-01-17 安徽大学 Fluorescent probe compound containing coumarin-thiadiazole Schiff base and preparation method and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2407686A (en) * 1943-04-21 1946-09-17 Frances R Ruskin Sulphanilamide derivatives and method of preparing same
FR992364A (en) * 1944-06-22 1951-10-17 Prod De Chimie Organique De La Process for the preparation of water soluble sulfanilamides
GB844946A (en) * 1957-03-14 1960-08-17 American Cyanamid Co New 2-(n-substituted)-acylamino-1,3,4-thiadiazole-5-sulfonamides
US4255182A (en) * 1980-03-10 1981-03-10 Velsicol Chemical Corporation Aminosulfonylthiadiazolylureas
EP0063879A2 (en) * 1981-04-17 1982-11-03 Yale University Modified nucleotides and methods of preparing and using same
WO1984003285A1 (en) * 1983-02-22 1984-08-30 Molecular Biosystems Inc Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
EP0097373B1 (en) * 1982-06-23 1992-10-07 Enzo Biochem, Inc. Modified labeled nucleotides and polynucleotides and methods of preparing, utilizing and detecting same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4134792A (en) * 1976-12-06 1979-01-16 Miles Laboratories, Inc. Specific binding assay with an enzyme modulator as a labeling substance

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2407686A (en) * 1943-04-21 1946-09-17 Frances R Ruskin Sulphanilamide derivatives and method of preparing same
FR992364A (en) * 1944-06-22 1951-10-17 Prod De Chimie Organique De La Process for the preparation of water soluble sulfanilamides
GB844946A (en) * 1957-03-14 1960-08-17 American Cyanamid Co New 2-(n-substituted)-acylamino-1,3,4-thiadiazole-5-sulfonamides
US4255182A (en) * 1980-03-10 1981-03-10 Velsicol Chemical Corporation Aminosulfonylthiadiazolylureas
EP0063879A2 (en) * 1981-04-17 1982-11-03 Yale University Modified nucleotides and methods of preparing and using same
EP0097373B1 (en) * 1982-06-23 1992-10-07 Enzo Biochem, Inc. Modified labeled nucleotides and polynucleotides and methods of preparing, utilizing and detecting same
WO1984003285A1 (en) * 1983-02-22 1984-08-30 Molecular Biosystems Inc Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACS, vol. 78, no. 13, 2nd April 1973, page 351, column 2, abstract no. 83315r, Columbus, Ohio, US; H. GOYA et al.: "Sulfanilamides as inhibitors of oxidations of ammoniacal nitrogen in soils "; & JP-A-47 004 966 (YOSHITOMI PHARMACEUTICAL IND.,LTD). *
CHEMICAL ABSTRACTS, vol. 98, no. 5, 31th January 1983, page 297, column 1, abstract no. 30198m, Columbus, Ohio, US: J.N. SHEPARD et al. "The measurement of human erythrocyte carbonic anhydrase I by the enzyme-linked immunosorbent assay (ELISA)"; & CLIN. BIOCHEM. (OTTAWA), vol. 15, no. 5, 1982, 248-251. *

Also Published As

Publication number Publication date
EP0210021A2 (en) 1987-01-28
JPS6214800A (en) 1987-01-23
CA1294899C (en) 1992-01-28
US4780405A (en) 1988-10-25

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Inventor name: MUSSO, GARY FRED

Inventor name: GHOSH, SOUMITRA SHANKAR

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Inventor name: KAISER, EMIL THOMAS

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