NO20022171L - Process for the synthesis of nucleic acid - Google Patents

Process for the synthesis of nucleic acid

Info

Publication number
NO20022171L
NO20022171L NO20022171A NO20022171A NO20022171L NO 20022171 L NO20022171 L NO 20022171L NO 20022171 A NO20022171 A NO 20022171A NO 20022171 A NO20022171 A NO 20022171A NO 20022171 L NO20022171 L NO 20022171L
Authority
NO
Norway
Prior art keywords
nucleic acid
synthesis
oligonucleotide
synthesizing
primer
Prior art date
Application number
NO20022171A
Other languages
Norwegian (no)
Other versions
NO20022171D0 (en
NO331732B1 (en
Inventor
Tsugunori Notomi
Tetsu Hase
Original Assignee
Eiken Chemical
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=14237231&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO20022171(L) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/JP1999/006213 external-priority patent/WO2000028082A1/en
Application filed by Eiken Chemical filed Critical Eiken Chemical
Publication of NO20022171D0 publication Critical patent/NO20022171D0/en
Publication of NO20022171L publication Critical patent/NO20022171L/en
Publication of NO331732B1 publication Critical patent/NO331732B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

Foreliggende oppfinnelse gjelder et oligonukleotid med en ny struktur og en fremgangsmåte for syntese av en nukleinsyre ved anvendelse av oligonukleotidet som primer. I sin 5'-ende er oligonukleotidet utstyrt med en basesekvens som i det vesentlige er identisk med området som skal syntetiseres ved anvendelse av primeren som utgangspunkt for syntesen. Således kan en nukleinsyre syntetiseres i en iso-term reaksjon ved anvendelse av en enkel reagensblanding. Videre tilveie-bringes en fremgangsmåte for syntese av en nukleinsyre med høy spesifisitet, basert på den ovenfor beskrevne fremgangsmåte for syntese av en nukleinsyre .The present invention relates to an oligonucleotide having a novel structure and a method for synthesizing a nucleic acid using the oligonucleotide as a primer. At its 5 'end, the oligonucleotide is provided with a base sequence that is substantially identical to the region to be synthesized using the primer as a starting point for the synthesis. Thus, a nucleic acid can be synthesized in an isothermal reaction using a simple reagent mixture. Furthermore, a method for synthesizing a high specificity nucleic acid is provided, based on the above-described method for synthesizing a nucleic acid.

NO20022171A 1999-11-08 2002-05-07 Procedure for Synthesis of Nucleic Acid NO331732B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/JP1999/006213 WO2000028082A1 (en) 1998-11-09 1999-11-08 Process for synthesizing nucleic acid
PCT/JP2000/001919 WO2001034790A1 (en) 1999-11-08 2000-03-28 Method for synthesizing nucleic acid

Publications (3)

Publication Number Publication Date
NO20022171D0 NO20022171D0 (en) 2002-05-07
NO20022171L true NO20022171L (en) 2002-07-04
NO331732B1 NO331732B1 (en) 2012-03-12

Family

ID=14237231

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20022171A NO331732B1 (en) 1999-11-08 2002-05-07 Procedure for Synthesis of Nucleic Acid

Country Status (9)

Country Link
KR (1) KR100612551B1 (en)
CN (2) CN100393875C (en)
BR (1) BR0015382B1 (en)
CA (1) CA2390309C (en)
IL (1) IL149446A0 (en)
NO (1) NO331732B1 (en)
RU (1) RU2252964C2 (en)
WO (1) WO2001034790A1 (en)
ZA (1) ZA200203293B (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
EP1275715B1 (en) * 2000-04-07 2015-07-15 Eiken Kagaku Kabushiki Kaisha Method for amplifyingand detecting a nucleic acid by using double-stranded nucleic acid as template
US7374913B2 (en) 2000-09-19 2008-05-20 Eiken Kagaku Kabushiki Kaisha Method for synthesizing polynucleotides
KR100806680B1 (en) * 2003-11-21 2008-02-26 한국표준과학연구원 Multiplex PCR mixtures for Testing the Performance of Thermocyclers
WO2010108325A1 (en) * 2009-03-26 2010-09-30 厦门艾德生物医药科技有限公司 Loop-shaped primer employed in nucleic acid amplification and the use thereof
CN101671674B (en) * 2009-03-27 2010-09-22 郑立谋 Annular primer for amplification of nucleic acid and application thereof
TWI600766B (en) * 2012-08-09 2017-10-01 財團法人工業技術研究院 Kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence
DK3553175T3 (en) 2013-03-13 2021-08-23 Illumina Inc PROCEDURE FOR MAKING A NUCLEIC ACID SEQUENCE LIBRARY
KR20150132481A (en) * 2013-03-15 2015-11-25 테라노스, 인코포레이티드 Nucleic Acid Amplification
ES2908751T3 (en) 2013-03-15 2022-05-03 Labrador Diagnostics Llc Nucleic acid amplification
WO2015075198A1 (en) * 2013-11-22 2015-05-28 Orion Diagnostica Oy Detection of nucleic acids by strand invasion based amplification
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
IL299976A (en) * 2014-10-17 2023-03-01 Illumina Cambridge Ltd Contiguity preserving transposition
EP3215260B1 (en) 2014-11-03 2020-01-15 Tangen Biosciences Inc. Apparatus and method for cell, spore, or virus capture and disruption
AU2015362617B2 (en) 2014-12-15 2022-03-03 Cepheid Exponential base-greater-than-2 nucleic acid amplification
WO2018132939A1 (en) * 2017-01-17 2018-07-26 中国科学院过程工程研究所 Method for synthesizing nucleic acid under constant temperature
EP3476938B1 (en) * 2017-09-14 2020-05-13 Zhongke Xinray (Suzhou) Biological Science Technologies Co., Ltd. Method and kit for synthesizing nucleic acid under constant temperature conditions
EP3874064A1 (en) 2018-10-29 2021-09-08 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
EP3911765A1 (en) 2019-01-15 2021-11-24 3M Innovative Properties Company Loop-mediated isothermal amplification primers for shiga toxin producing e. coli (stec) detection
WO2021165828A1 (en) 2020-02-17 2021-08-26 3M Innovative Properties Company Loop-mediated isothermal amplification primers for vibrio parahaemolyticus detection and uses thereof
WO2021252836A1 (en) 2020-06-12 2021-12-16 Sherlock Biosciences Crispr-based sars-cov-2 detection
US20220059187A1 (en) 2020-08-07 2022-02-24 Oxford Nanopore Technologies Limited Methods of detecting nucleic acid barcodes
CN113201583B (en) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 Method for synthesizing nucleic acid under constant temperature condition, kit and application
WO2022226870A1 (en) * 2021-04-29 2022-11-03 中国科学院大学宁波生命与健康产业研究院 Method for synthesizing nucleic acid under constant temperature conditions, kit, and application

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4213029A1 (en) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh METHOD FOR THE SPECIFIC REPRODUCTION OF NUCLEIN'S SEQUENCES
DK0549107T3 (en) * 1991-10-11 1998-08-31 Dade Behring Marburg Gmbh Process for Preparing a Polynucleotide for Use in Single Primer Amplification and Phosphorothioate-Containing Oils
WO1993017127A1 (en) * 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
FR2721945B1 (en) * 1994-07-04 1996-10-18 David Fabrice GENE ENHANCEMENT, A METHOD OF ISOTHERMAL GENE AMPLIFICATION AND ITS APPLICATIONS
FR2726277B1 (en) * 1994-10-28 1996-12-27 Bio Merieux OLIGONUCLEOTIDE FOR USE AS PRIMER IN AN AMPLIFICATION METHOD BASED ON REPLICATION WITH MOVEMENT OF STRAND
ATE317023T1 (en) * 1999-11-08 2006-02-15 Eiken Chemical METHOD FOR DETECTING VARIATIONS OR POLYMORPHISMS

Also Published As

Publication number Publication date
NO20022171D0 (en) 2002-05-07
ZA200203293B (en) 2003-03-26
RU2252964C2 (en) 2005-05-27
KR100612551B1 (en) 2006-08-11
RU2002115268A (en) 2004-01-27
CN1876843A (en) 2006-12-13
CN1876843B (en) 2012-09-05
KR20020064896A (en) 2002-08-10
CA2390309C (en) 2012-09-25
BR0015382B1 (en) 2014-04-29
CA2390309A1 (en) 2001-05-17
WO2001034790A1 (en) 2001-05-17
NO331732B1 (en) 2012-03-12
CN100393875C (en) 2008-06-11
CN1420928A (en) 2003-05-28
BR0015382A (en) 2002-07-02
IL149446A0 (en) 2002-11-10

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