AU2001288065A1 - Method of synthesizing polynucleotide - Google Patents

Method of synthesizing polynucleotide

Info

Publication number
AU2001288065A1
AU2001288065A1 AU2001288065A AU8806501A AU2001288065A1 AU 2001288065 A1 AU2001288065 A1 AU 2001288065A1 AU 2001288065 A AU2001288065 A AU 2001288065A AU 8806501 A AU8806501 A AU 8806501A AU 2001288065 A1 AU2001288065 A1 AU 2001288065A1
Authority
AU
Australia
Prior art keywords
loops
polynucleotides
isothermal
synthesizing polynucleotide
templates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001288065A
Inventor
Kentaro Nagamine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18768163&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2001288065(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Publication of AU2001288065A1 publication Critical patent/AU2001288065A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.
AU2001288065A 2000-09-19 2001-09-19 Method of synthesizing polynucleotide Abandoned AU2001288065A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000283862 2000-09-19
JP2000-283862 2000-09-19
PCT/JP2001/008142 WO2002024902A1 (en) 2000-09-19 2001-09-19 Method of synthesizing polynucleotide

Publications (1)

Publication Number Publication Date
AU2001288065A1 true AU2001288065A1 (en) 2002-04-02

Family

ID=18768163

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001288065A Abandoned AU2001288065A1 (en) 2000-09-19 2001-09-19 Method of synthesizing polynucleotide

Country Status (10)

Country Link
US (3) US7374913B2 (en)
EP (2) EP2031058B1 (en)
JP (2) JP4918210B2 (en)
CN (1) CN1222614C (en)
AT (1) ATE437225T1 (en)
AU (1) AU2001288065A1 (en)
DE (1) DE60139331D1 (en)
ES (2) ES2327712T3 (en)
HK (1) HK1055445A1 (en)
WO (1) WO2002024902A1 (en)

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US7374913B2 (en) * 2000-09-19 2008-05-20 Eiken Kagaku Kabushiki Kaisha Method for synthesizing polynucleotides
ES2382868T3 (en) 2002-02-20 2012-06-14 Sysmex Corporation Primers for nucleic acid amplification in the constituent gene mRNA detection and test method using these primers
ATE516365T1 (en) 2002-05-21 2011-07-15 Sysmex Corp NUCLEIC ACID AMPLIFICATION PRIMER FOR DETECTING CYTOKERATINS AND EXAMINATION METHODS USING THE PRIMERS
US7803579B2 (en) * 2002-10-29 2010-09-28 Riken Process for amplifying nucleic acid
US7399588B2 (en) 2003-06-27 2008-07-15 Eiken Kagaku Kabushiki Kaisha Method for detecting SARS coronavirus
JP2005110587A (en) * 2003-10-08 2005-04-28 Sapporo Breweries Ltd Primer for detecting alicyclobacillus bacteria
US8206902B2 (en) * 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
JP2008000002A (en) * 2004-09-30 2008-01-10 Sysmex Corp NUCLEIC ACID AMPLIFICATION PRIMER FOR DETECTING RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS-1A) GENE AND/OR mRNA OF THE GENE AND INSPECTION METHOD USING THE GENE AND/OR mRNA OF THE GENE AS INTERNAL STANDARD
US8043813B2 (en) 2004-11-01 2011-10-25 Japan As Represented By Director-General Of National Institute Of Infectious Diseases Method of detecting H5 or H7 avian influenza virus
JP2006254784A (en) * 2005-03-17 2006-09-28 Institute Of Physical & Chemical Research Method for decreasing nonspecific amplification from primer dimer
FR2885140B1 (en) 2005-04-29 2010-12-31 Millipore Corp METHOD FOR DETECTING AND CHARACTERIZING MICROORGARISTS ON A MEMBRANE
US8956850B2 (en) * 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
WO2007069331A1 (en) * 2005-12-16 2007-06-21 Eiken Kagaku Kabushiki Kaisha Method for detection of human immunodeficiency virus type 1
JP4580875B2 (en) * 2006-01-20 2010-11-17 株式会社東芝 Primer design method for nucleic acid detection method
JP4881623B2 (en) 2006-01-27 2012-02-22 シスメックス株式会社 CEA nucleic acid amplification primer, primer set, and cancer diagnosis support method
MX2009007280A (en) * 2007-01-17 2009-09-08 Meridian Bioscience Inc Stable reagents and kits useful in loop-mediated isothermal amplification (lamp).
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
JP4410269B2 (en) 2007-03-28 2010-02-03 株式会社東芝 Nucleic acid primer set, kit for detecting genotype of N-acetyltransferase 2 (NAT2), and detection method using the primer set
JP4410268B2 (en) 2007-03-28 2010-02-03 株式会社東芝 Nucleic acid primer set and nucleic acid probe for detecting the genotype of methylenetetrahydrofolate reductase (MTHFR)
JP4490988B2 (en) 2007-04-26 2010-06-30 株式会社東芝 Nucleic acid primer set and kit for detecting genotype of serum amyloid A1 (SAA1), and detection method using the primer set
WO2009054510A1 (en) 2007-10-25 2009-04-30 Riken Isothermal amplification method and dna polymerase used in the same
JPWO2009119331A1 (en) * 2008-03-28 2011-07-21 シスメックス株式会社 Reagent containing primer for detection of cytokeratin 7 mRNA
CN103923989B (en) 2008-05-28 2016-04-27 花王株式会社 The detection method of thermotolerant bacterium
EP2325309B1 (en) 2008-05-29 2014-10-22 Kao Corporation Method for detection of paecilomyces variotii
US20110236900A1 (en) 2008-11-27 2011-09-29 Riken Novel muts protein and method for determing mutation using the same
CN102246043B (en) 2008-12-25 2016-06-08 环球生物研究株式会社 The preprocess method of sample and the assay method of bio-related substance
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
JP5299981B1 (en) 2011-09-08 2013-09-25 独立行政法人理化学研究所 Primer set, target nucleic acid sequence amplification method and mutant nucleic acid detection method using the same
WO2013065574A1 (en) 2011-10-31 2013-05-10 栄研化学株式会社 Method for detecting target nucleic acid
WO2014085687A1 (en) 2012-11-28 2014-06-05 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
US9677981B2 (en) 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
US10351918B2 (en) 2013-10-30 2019-07-16 Green Life Biotech, Llc Pathogenesis quantification systems and treatment methods for citrus greening blight
WO2016011280A1 (en) 2014-07-16 2016-01-21 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
WO2016073353A1 (en) 2014-11-03 2016-05-12 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
WO2016098892A1 (en) * 2014-12-19 2016-06-23 国立大学法人 長崎大学 Primer set for detection of zaire ebola virus, assay kit, and amplification method
JP6605238B2 (en) * 2014-12-19 2019-11-13 国立大学法人 長崎大学 Zaire Ebola virus detection primer set, assay kit and amplification method
MY193444A (en) 2015-03-30 2022-10-13 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
JPWO2017212904A1 (en) * 2016-06-06 2019-04-11 国立大学法人 宮崎大学 Rapid detection method of African swine fever virus using LAMP method combining multiple primer sets
JP7176682B2 (en) 2018-02-22 2022-11-22 佐藤製薬株式会社 A primer set for detecting a trichophyton gene by the LAMP method, a kit containing the same, and a method for detecting trichophyton using them
CN109658982B (en) * 2018-12-25 2020-06-19 人和未来生物科技（长沙）有限公司 Primer design method and system for gene sequencing
SG10201910254YA (en) 2019-11-04 2021-06-29 Denka Company Ltd Nucleic Acid Detection Method Using Lamp And Probe Detection
CN110878367A (en) * 2019-12-05 2020-03-13 华南农业大学 Novel CPA method, primer group and kit capable of detecting SNP
CN115151656A (en) 2020-02-27 2022-10-04 荣研化学株式会社 Method for detecting single-stranded RNA virus
JP7313537B2 (en) 2020-03-05 2023-07-24 栄研化学株式会社 Method for detecting coronavirus (SARS-CoV-2)
CN115867653A (en) 2020-03-13 2023-03-28 艾优实验室公司 Loop primer and loop-de-loop method for detecting target nucleic acid
EP4146833A1 (en) 2020-05-05 2023-03-15 Genomtec SA Diagnostic primers, kits and methods for viral detection
WO2021252836A1 (en) 2020-06-12 2021-12-16 Sherlock Biosciences Crispr-based sars-cov-2 detection
WO2022029449A1 (en) 2020-08-07 2022-02-10 Oxford Nanopore Technologies Limited Methods of identifying nucleic acid barcodes
CN112553300A (en) * 2020-10-15 2021-03-26 德歌生物技术(山东)有限公司 Primer design method, primer and method for isothermal amplification of nucleic acid fragment
EP3992300A1 (en) 2020-10-30 2022-05-04 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk Onderzoek TNO Sample pre-treatment for isothermal amplification
CA3210883A1 (en) 2021-02-09 2022-08-18 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
US11788129B2 (en) 2021-02-23 2023-10-17 Industrial Technology Research Institute Nucleic acid detection kit and nucleic acid detection method
CN113201583B (en) * 2021-04-29 2022-02-08 国科宁波生命与健康产业研究院 Method for synthesizing nucleic acid under constant temperature condition, kit and application
EP4105327A4 (en) * 2021-04-29 2023-01-18 Ningbo Institute of Life and Health Industry University of Chinese Academy of Sciences Method for synthesizing nucleic acid under constant temperature conditions, kit, and application
CN118434883A (en) 2021-12-24 2024-08-02 豪夫迈·罗氏有限公司 Improved detection of LAMP amplification products

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
ES2294862T5 (en) * 1998-11-09 2011-04-12 Eiken Kagaku Kabushiki Kaisha NUCLEIC ACID SYNTHESIS PROCEDURE.
CA2390309C (en) 1999-11-08 2012-09-25 Eiken Kagaku Kabushiki Kaisha Method for synthesizing the nucleic acid
AU1057801A (en) 1999-11-08 2001-06-06 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
US8017357B2 (en) * 2000-04-07 2011-09-13 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US7374913B2 (en) * 2000-09-19 2008-05-20 Eiken Kagaku Kabushiki Kaisha Method for synthesizing polynucleotides

Also Published As

Publication number Publication date
CN1222614C (en) 2005-10-12
US20080213842A1 (en) 2008-09-04
CN1474870A (en) 2004-02-11
JPWO2002024902A1 (en) 2004-01-29
EP2031058A1 (en) 2009-03-04
EP1327679A4 (en) 2005-06-01
US7846695B2 (en) 2010-12-07
EP2031058B1 (en) 2012-07-18
US7374913B2 (en) 2008-05-20
EP1327679B1 (en) 2009-07-22
ES2390242T3 (en) 2012-11-07
JP4918210B2 (en) 2012-04-18
ATE437225T1 (en) 2009-08-15
US20080213790A1 (en) 2008-09-04
US20040038253A1 (en) 2004-02-26
JP2012085652A (en) 2012-05-10
US7851186B2 (en) 2010-12-14
DE60139331D1 (en) 2009-09-03
WO2002024902A1 (en) 2002-03-28
ES2327712T3 (en) 2009-11-03
EP1327679A1 (en) 2003-07-16
HK1055445A1 (en) 2004-01-09

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