MX2012004627A - Composicion, metodo y kit para la deteccion de bacterias mediante secuenciacion. - Google Patents

Composicion, metodo y kit para la deteccion de bacterias mediante secuenciacion.

Info

Publication number
MX2012004627A
MX2012004627A MX2012004627A MX2012004627A MX2012004627A MX 2012004627 A MX2012004627 A MX 2012004627A MX 2012004627 A MX2012004627 A MX 2012004627A MX 2012004627 A MX2012004627 A MX 2012004627A MX 2012004627 A MX2012004627 A MX 2012004627A
Authority
MX
Mexico
Prior art keywords
sequencing
kit
composition
detecting bacteria
detecting
Prior art date
Application number
MX2012004627A
Other languages
English (en)
Inventor
Jesus Mingorance Cruz
Pedro Manuel Franco De Sarabia Rosado
Pablo Castan Garcia
Original Assignee
Biotools Biotechnological & Medical Lab S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotools Biotechnological & Medical Lab S A filed Critical Biotools Biotechnological & Medical Lab S A
Publication of MX2012004627A publication Critical patent/MX2012004627A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención describe un método para detectar la presencia y tipo de microorganismo presente en una muestra mediante técnicas de estabilización y de secuenciación, y posterior análisis de microsecuencias en genes codificantes para el RNA ribosomal de mayor conservación, y sobre áreas específicas de la región 1 6-S con valor taxonómico.
MX2012004627A 2009-10-22 2009-10-22 Composicion, metodo y kit para la deteccion de bacterias mediante secuenciacion. MX2012004627A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/ES2009/000507 WO2011048227A1 (es) 2009-10-22 2009-10-22 Composición, método y kit para la detección de bacterias mediante secuenciación

Publications (1)

Publication Number Publication Date
MX2012004627A true MX2012004627A (es) 2012-05-23

Family

ID=43899855

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012004627A MX2012004627A (es) 2009-10-22 2009-10-22 Composicion, metodo y kit para la deteccion de bacterias mediante secuenciacion.

Country Status (7)

Country Link
US (1) US20130157265A1 (es)
EP (1) EP2492352A4 (es)
BR (1) BR112012009023A2 (es)
CA (1) CA2775273A1 (es)
IN (1) IN2012DN02587A (es)
MX (1) MX2012004627A (es)
WO (1) WO2011048227A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110592244A (zh) * 2013-07-25 2019-12-20 德诚分子诊断 用于检测细菌污染的方法和组合物
GB201417372D0 (en) 2014-10-01 2014-11-12 Arcis Biotechnology Holdings Ltd Methods and kits
CN109971844A (zh) * 2019-05-15 2019-07-05 四川剑南春（集团）有限责任公司 一种快速鉴定酿酒厌氧微生物的方法
EP3901286A1 (en) 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
EP4055189A1 (en) 2019-11-04 2022-09-14 Mirnax Biosens, S.L. Bivalent reverse primer

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE3682047D1 (de) 1985-07-09 1991-11-21 Quadrant Bioresources Ltd Beschuetzung von proteinen und aehnlichem.
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB9010742D0 (en) 1990-05-14 1990-07-04 Quadrant Bioresources Ltd Stabilization of biological macromolecular substances
AU2309692A (en) 1991-07-03 1993-02-11 Cryolife, Inc. Method for stabilization of biomaterials
JPH09508003A (ja) * 1993-07-23 1997-08-19 ハイセック，インコーポレイション 未知の生物をスクリーニングするための方法
NL9301957A (nl) * 1993-11-11 1995-06-01 U Gene Research Bv Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen.
US5955448A (en) 1994-08-19 1999-09-21 Quadrant Holdings Cambridge Limited Method for stabilization of biological substances during drying and subsequent storage and compositions thereof
JPH08131171A (ja) 1994-11-11 1996-05-28 Asahi Chem Ind Co Ltd 逆転写酵素の安定化組成物
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US5861251A (en) 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US7288371B1 (en) * 1996-12-23 2007-10-30 The University Of Chicago Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips
US6030818A (en) * 1997-12-22 2000-02-29 Bcm Developpement, Inc. Bacterial mass production of taxanes and paclitaxel
US6225289B1 (en) * 1998-12-10 2001-05-01 Genvec, Inc. Methods and compositions for preserving adenoviral vectors
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
WO2000066777A2 (en) 1999-04-30 2000-11-09 Qualicon, Inc. Assay based on the analysis of dna melting curves
AU2001269292A1 (en) * 2000-07-07 2002-01-21 Helen Lee Improved stability of hybridisation interactions in dipstick assays
GB0026424D0 (en) * 2000-10-28 2000-12-13 Ncimb Ltd Genetic analysis of microorganisms
US6699670B2 (en) 2001-03-01 2004-03-02 The Johns Hopkins University Quantitative assay for the simultaneous detection and speciation of bacterial infections
ES2180416B1 (es) * 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.
US20040023209A1 (en) * 2001-11-28 2004-02-05 Jon Jonasson Method for identifying microorganisms based on sequencing gene fragments
AU2003279664A1 (en) 2002-11-15 2004-06-15 Biotage Ab A method for inter-species differentiation and identification of a gram-positive bacteria
WO2005060345A2 (en) 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
US8076104B2 (en) * 2007-01-25 2011-12-13 Rogan Peter K Rapid and comprehensive identification of prokaryotic organisms
WO2009049007A2 (en) 2007-10-10 2009-04-16 Magellan Biosciences, Inc. Compositions, methods and systems for rapid identification of pathogenic nucleic acids

Also Published As

Publication number Publication date
EP2492352A1 (en) 2012-08-29
IN2012DN02587A (es) 2015-08-28
CA2775273A1 (en) 2011-04-28
US20130157265A1 (en) 2013-06-20
BR112012009023A2 (pt) 2019-09-24
EP2492352A4 (en) 2013-03-20
WO2011048227A1 (es) 2011-04-28

Similar Documents

Publication Publication Date Title
GB201317477D0 (en) Methods, compositions and kits for determing the presence/absnece of a variant nucleic acid sequence
MX341204B (es) Metodo para caracterizar almenos un microorganismo por medio de espectrometria de masa.
PL1895015T3 (pl) Sposób i zestaw do wykrywania gatunków bakteryjnych za pomocą analizy DNA
WO2012030136A3 (en) Oligonucleotides for detecting listeria spp. and use thereof
WO2009142735A3 (en) LUCIFERASE BIOSENSORS FOR cAMP
MX2012004627A (es) Composicion, metodo y kit para la deteccion de bacterias mediante secuenciacion.
EP2314680A4 (en) METHOD FOR THE AMPLIFICATION OF A TARGET NUCLEIC ACID SEQUENCE, METHOD FOR METHODIC DETECTION USING THE METHOD AND REAGENT FOR USE IN THE PROCEDURES
EP2491144A4 (en) METHODS, KITS AND REACTION MIXTURES FOR THE ANALYSIS OF SINGLE-STRENGTH NUCLEIC ACID SEQUENCES
ATE533838T1 (de) Verfahren zur verbesserten einführung von dna in bakterienzellen
BRPI0811490A2 (pt) Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucléicos através de nanopartículas metálicas funcionalizadas com oligonucleotídos modificados.
MY182940A (en) Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
WO2010092164A3 (en) Bacterial dna as markers of cardiovascular and/or metabolic disease
GB201021499D0 (en) Detection of quantative genetic differnces
UA118676C2 (uk) СПОСІБ ВИЯВЛЕННЯ РОСЛИНИ Zea mays, ЯКА МІСТИТЬ ЩОНАЙМЕНШЕ ОДИН АЛЕЛЬ, АСОЦІЙОВАНИЙ З ОПТИМІЗАЦІЄЮ ВОДОСПОЖИВАННЯ
WO2012135620A8 (en) Molecular gram stain
GB201317805D0 (en) Process controls for molecular assay
WO2014102761A8 (es) Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección
IN2012DN00172A (es)
WO2012151599A3 (de) Lamp-verfahren zum nachweisen von erwinia amylovora
HK1159692A1 (en) Method for the specific detection of low abundance rna species in a biological sample rna
TN2009000537A1 (en) Method for the identification of propane oxidizing bacteria
MX2015004809A (es) Pronostico del impacto de la dieta sobre las co-morbilidades relacionadas con la obesidad.
EP2450455A4 (en) METHOD FOR DETERMINING WHETHER OR NOT EPITHELIAL CELLS OF CANCER ORIGIN ARE PRESENT OR NOT IN A BIOLOGICAL SAMPLE, MOLECULAR MARKER AND NECESSARY THEREFOR
EP2358910A4 (en) NUCLEOTIDE SEQUENCES, METHODS AND KITS FOR DETECTING HPV
WO2011009975A3 (es) Método para la detección e identificación de especies de la familia engraulidae

Legal Events

Date Code Title Description
FA Abandonment or withdrawal