WO2012151599A3 - Lamp-verfahren zum nachweisen von erwinia amylovora - Google Patents

Lamp-verfahren zum nachweisen von erwinia amylovora Download PDF

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Publication number
WO2012151599A3
WO2012151599A3 PCT/AT2012/050060 AT2012050060W WO2012151599A3 WO 2012151599 A3 WO2012151599 A3 WO 2012151599A3 AT 2012050060 W AT2012050060 W AT 2012050060W WO 2012151599 A3 WO2012151599 A3 WO 2012151599A3
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WO
WIPO (PCT)
Prior art keywords
erwinia amylovora
sequence
lamp method
detecting
seq
Prior art date
Application number
PCT/AT2012/050060
Other languages
English (en)
French (fr)
Other versions
WO2012151599A2 (de
Inventor
Karl Stich
Thilo Fischer
Christian Gosch
Original Assignee
Technische Universität Wien
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT6402011A external-priority patent/AT510418B1/de
Priority claimed from ATA1076/2011A external-priority patent/AT511771B1/de
Application filed by Technische Universität Wien filed Critical Technische Universität Wien
Publication of WO2012151599A2 publication Critical patent/WO2012151599A2/de
Publication of WO2012151599A3 publication Critical patent/WO2012151599A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/101—Linear amplification, i.e. non exponential
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum Nachweisen von Erwinia amylovora in einer Probe, indem eine oder mehrere Erwinia amylovora-Sequenzen in der Probe unter Anwendung der LAMP-Methode mittels für diese Sequenz(en) spezifischer Primer amplifiziert und gegebenenfalls mit bekannten Sequenzen verglichen werden, mit dem Kennzeichen, dass als Referenzsequenz die genomische Sequenz der Basen 378996 bis 1379215 des Erwinia amylovora-Genoms, die für das hypothetische Protein AMY1267 des Stamms Ea273 kodiert, wie in Seq.-ID Nr. 1 dargelegt, verwendet wird und der aus den Seq.-ID Nrn. 2 bis 7 bestehende Satz spezifischer LAMP-Primer zur Amplifikation eingesetzt wird.
PCT/AT2012/050060 2011-05-06 2012-05-04 Lamp-verfahren zum nachweisen von erwinia amylovora WO2012151599A2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ATA640/2011 2011-05-06
AT6402011A AT510418B1 (de) 2011-05-06 2011-05-06 Lamp-verfahren zum nachweisen von erwinia amylovora
ATA1076/2011A AT511771B1 (de) 2011-07-22 2011-07-22 Verwendung einer bienenpopulation zur gewinnung von proben von pflanzen
ATA1076/2011 2011-07-22

Publications (2)

Publication Number Publication Date
WO2012151599A2 WO2012151599A2 (de) 2012-11-15
WO2012151599A3 true WO2012151599A3 (de) 2013-01-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT2012/050060 WO2012151599A2 (de) 2011-05-06 2012-05-04 Lamp-verfahren zum nachweisen von erwinia amylovora

Country Status (1)

Country Link
WO (1) WO2012151599A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104388577A (zh) * 2014-12-11 2015-03-04 山东出入境检验检疫局检验检疫技术中心 一种检测梨火疫病菌的环介导恒温扩增引物及试剂盒
KR102403327B1 (ko) * 2020-10-16 2022-05-31 대한민국 어위니아 피리폴리애 검출용 프라이머 세트 및 이의 용도
KR102403329B1 (ko) * 2020-10-16 2022-05-30 대한민국 어위니아 아밀로보라 검출용 프라이머 세트 및 이의 용도
KR102586238B1 (ko) * 2021-06-16 2023-10-11 대한민국 어위니아 아밀로보라 개체 판별용 프라이머 세트 및 이의 용도

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT510418A4 (de) * 2011-05-06 2012-04-15 Univ Wien Tech Lamp-verfahren zum nachweisen von erwinia amylovora

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT510418A4 (de) * 2011-05-06 2012-04-15 Univ Wien Tech Lamp-verfahren zum nachweisen von erwinia amylovora

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ABOUBAKR MORADI ET AL: "Development and evaluation of a loop-mediated isothermal amplification assay for detection ofbased on chromosomal DNA", EUROPEAN JOURNAL OF PLANT PATHOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 133, no. 3, 2 February 2012 (2012-02-02), pages 609 - 620, XP035060799, ISSN: 1573-8469, DOI: 10.1007/S10658-012-9939-Y *
CHRISTIAN GOSCH ET AL: "Blue EaLAMP-a specific and sensitive method for visual detection of genomic Erwinia amylovora DNA", EUROPEAN JOURNAL OF PLANT PATHOLOGY, 19 August 2012 (2012-08-19) - 19 August 2012 (2012-08-19), XP055041522, ISSN: 0929-1873, DOI: 10.1007/s10658-012-0059-5 *
GOSCH C ET AL: "New bactericides against fire blight (erwinia amylovora) and development of a fast bee-erwinia monitoring system", ACTA HORTICULTURAE, INTERNATIONAL SOCIETY FOR HORTICULTURAL SCIENCE, BE, vol. 917, 22 August 2010 (2010-08-22) - 1 January 2011 (2011-01-01), pages 139 - 142, XP009163872, ISSN: 0567-7572 *
GOTTSBERGER R A: "Development and evaluation of a real-time PCR assay targeting chromosomal DNA of Erwinia amylovora", LETTERS IN APPLIED MICROBIOLOGY, vol. 51, no. 3, September 2010 (2010-09-01), pages 285 - 292, XP002685948, ISSN: 0266-8254 *
LÃ3PEZ: "Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses?", CURRENT ISSUES IN MOLECULAR BIOLOGY, vol. 11, no. 1, 1 January 2009 (2009-01-01), pages 13, XP055041602, ISSN: 1467-3037 *
TEMPLE TODD N ET AL: "Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Erwinia amylovora on Pear and Apple Fruit Flowers", PLANT DISEASE, vol. 95, no. 4, April 2011 (2011-04-01), pages 423 - 430, XP002685947, ISSN: 0191-2917 *

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Publication number Publication date
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