MX354465B - Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto. - Google Patents

Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto.

Info

Publication number
MX354465B
MX354465B MX2014010738A MX2014010738A MX354465B MX 354465 B MX354465 B MX 354465B MX 2014010738 A MX2014010738 A MX 2014010738A MX 2014010738 A MX2014010738 A MX 2014010738A MX 354465 B MX354465 B MX 354465B
Authority
MX
Mexico
Prior art keywords
nucleic acid
acid sequence
target nucleic
detection
nucleotide variation
Prior art date
Application number
MX2014010738A
Other languages
English (en)
Other versions
MX2014010738A (es
Inventor
Jong Yoon Chun
Young Jo Lee
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of MX2014010738A publication Critical patent/MX2014010738A/es
Publication of MX354465B publication Critical patent/MX354465B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere en general, a un nuevo método y un kit para detectar variaciones de nucleótidos por un ensayo de PTOCE (Desdoblamiento y Extensión de PTO) con PTO-NV. Además, la presente invención se dirige a un nuevo método y un kit para detectar una variación del nucleótido en una secuencia de ácido nucleico de objetivo por un ensayo PTOCE con PTO-NV que tiene una porción de apareamiento sin base.
MX2014010738A 2012-03-05 2013-02-25 Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto. MX354465B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261606713P 2012-03-05 2012-03-05
US201261613195P 2012-03-20 2012-03-20
US201261668628P 2012-07-06 2012-07-06
PCT/KR2013/001492 WO2013133561A1 (en) 2012-03-05 2013-02-25 Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay

Publications (2)

Publication Number Publication Date
MX2014010738A MX2014010738A (es) 2015-06-17
MX354465B true MX354465B (es) 2018-03-06

Family

ID=49116988

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014010738A MX354465B (es) 2012-03-05 2013-02-25 Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto.

Country Status (13)

Country Link
US (1) US9650665B2 (es)
EP (1) EP2823061B1 (es)
JP (1) JP5976849B2 (es)
KR (1) KR101794981B1 (es)
CN (1) CN104245959B (es)
AU (1) AU2013228226B2 (es)
BR (1) BR112014021993B8 (es)
CA (1) CA2864523C (es)
ES (1) ES2669244T3 (es)
MX (1) MX354465B (es)
RU (1) RU2620955C2 (es)
WO (1) WO2013133561A1 (es)
ZA (1) ZA201405894B (es)

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AU2014200958B2 (en) * 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
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CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
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CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
CN110273012B (zh) * 2018-03-13 2022-11-04 厦门大学 一种检测败血症病原体的方法
CN110273013B (zh) * 2018-03-13 2022-11-01 厦门大学 一种检测呼吸道病原体的方法
KR102220701B1 (ko) 2018-09-28 2021-03-03 (주)나노헬릭스 형광기반의 다중 융해 분석을 이용한 증폭 산물의 다중 분석방법
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트
US20240301520A1 (en) * 2021-03-05 2024-09-12 Seegene, Inc. METHOD FOR DETECTION OF SARS-CoV-2 MUTATIONS
MX2023014845A (es) * 2021-06-17 2024-04-24 Seegene Inc Deteccion de multiples acidos nucleicos objetivo utilizando multiples temperaturas de deteccion.

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Also Published As

Publication number Publication date
CN104245959B (zh) 2017-10-13
BR112014021993A2 (pt) 2017-07-11
MX2014010738A (es) 2015-06-17
CA2864523A1 (en) 2013-09-12
WO2013133561A1 (en) 2013-09-12
RU2620955C2 (ru) 2017-05-30
ZA201405894B (en) 2016-08-31
US9650665B2 (en) 2017-05-16
RU2014138951A (ru) 2016-04-20
US20150167061A1 (en) 2015-06-18
BR112014021993B8 (pt) 2022-03-03
EP2823061A1 (en) 2015-01-14
EP2823061A4 (en) 2015-11-04
EP2823061B1 (en) 2018-02-14
CA2864523C (en) 2019-02-05
BR112014021993B1 (pt) 2022-01-11
CN104245959A (zh) 2014-12-24
KR101794981B1 (ko) 2017-11-09
AU2013228226A1 (en) 2014-09-04
ES2669244T3 (es) 2018-05-24
JP2015520603A (ja) 2015-07-23
KR20140123561A (ko) 2014-10-22
AU2013228226B2 (en) 2016-06-02
JP5976849B2 (ja) 2016-08-24

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