MX338076B - Deteccion cuantitativa, altamente multiplexada de acidos nucleicos. - Google Patents

Deteccion cuantitativa, altamente multiplexada de acidos nucleicos.

Info

Publication number
MX338076B
MX338076B MX2014005912A MX2014005912A MX338076B MX 338076 B MX338076 B MX 338076B MX 2014005912 A MX2014005912 A MX 2014005912A MX 2014005912 A MX2014005912 A MX 2014005912A MX 338076 B MX338076 B MX 338076B
Authority
MX
Mexico
Prior art keywords
nucleic acids
quantitative
highly multiplexed
multiplexed detection
methods
Prior art date
Application number
MX2014005912A
Other languages
English (en)
Other versions
MX2014005912A (es
Inventor
Kris Scaboo
Patrick Martin
Brad Taft
Jason La
Original Assignee
Nvs Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2012/025699 external-priority patent/WO2012112925A2/en
Application filed by Nvs Technologies Inc filed Critical Nvs Technologies Inc
Publication of MX2014005912A publication Critical patent/MX2014005912A/es
Publication of MX338076B publication Critical patent/MX338076B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a métodos de detección y cuantificación de ácidos nucleicos blanco de muestras en reacciones en una sola cámara multiplexada. Se proporcionan cámaras que incorporan consumibles optimizadas para reducir la señal de fondo próxima a matrices de alta eficiencia, así como métodos de uso. Dispositivos y sistemas configurados para utilizar los consumibles para la práctica de los métodos son una característica de la invención.
MX2014005912A 2011-11-17 2012-08-16 Deteccion cuantitativa, altamente multiplexada de acidos nucleicos. MX338076B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161561198P 2011-11-17 2011-11-17
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
US13/399,872 US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids

Publications (2)

Publication Number Publication Date
MX2014005912A MX2014005912A (es) 2014-09-04
MX338076B true MX338076B (es) 2016-04-01

Family

ID=48430026

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014005912A MX338076B (es) 2011-11-17 2012-08-16 Deteccion cuantitativa, altamente multiplexada de acidos nucleicos.

Country Status (12)

Country Link
EP (1) EP2780479A4 (es)
JP (1) JP2014533502A (es)
KR (1) KR20140094007A (es)
CN (1) CN104093857A (es)
AU (1) AU2012337389A1 (es)
BR (1) BR112014011937A2 (es)
CA (1) CA2855953A1 (es)
HK (1) HK1199746A1 (es)
IL (1) IL232586A0 (es)
MX (1) MX338076B (es)
SG (1) SG11201402341QA (es)
WO (1) WO2013074163A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6050555B2 (ja) * 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
WO2016168640A1 (en) 2015-04-16 2016-10-20 William Marsh Rice University Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species
CN110016500A (zh) * 2018-01-10 2019-07-16 深圳闪量科技有限公司 表面探针定量pcr方法
AU2020388573A1 (en) * 2019-11-21 2022-06-23 10X Genomics, Inc, Spatial analysis of analytes
CN111808989A (zh) * 2020-06-18 2020-10-23 重庆浦洛通基因医学研究院有限公司 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法
JP2023545506A (ja) 2020-10-15 2023-10-30 ジェネンテック， インコーポレイテッド 多重化された標的結合候補スクリーニング分析

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
WO2003042353A2 (en) * 2001-07-17 2003-05-22 Stratagene Methods for detection of a target nucleic acid by capture using multi-subunit probes
WO2005010199A2 (en) * 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
WO2005068660A1 (en) * 2003-12-19 2005-07-28 Beckman Coulter, Inc. Solid-phase multiplexed invader assay
CA2672246A1 (en) * 2006-06-30 2008-03-13 Rosetta Genomics Ltd Detecting nucleic acids
EP2118310B1 (en) * 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
US20120040853A1 (en) * 2008-11-21 2012-02-16 Koninklijke Philips Electronics N.V. Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes

Also Published As

Publication number Publication date
JP2014533502A (ja) 2014-12-15
CN104093857A (zh) 2014-10-08
BR112014011937A2 (pt) 2017-05-30
HK1199746A1 (en) 2015-07-17
EP2780479A4 (en) 2015-04-08
SG11201402341QA (en) 2014-06-27
EP2780479A1 (en) 2014-09-24
KR20140094007A (ko) 2014-07-29
IL232586A0 (en) 2014-06-30
MX2014005912A (es) 2014-09-04
AU2012337389A1 (en) 2014-06-19
WO2013074163A1 (en) 2013-05-23
CA2855953A1 (en) 2013-05-23

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