MX338076B - Deteccion cuantitativa, altamente multiplexada de acidos nucleicos. - Google Patents
Deteccion cuantitativa, altamente multiplexada de acidos nucleicos.Info
- Publication number
- MX338076B MX338076B MX2014005912A MX2014005912A MX338076B MX 338076 B MX338076 B MX 338076B MX 2014005912 A MX2014005912 A MX 2014005912A MX 2014005912 A MX2014005912 A MX 2014005912A MX 338076 B MX338076 B MX 338076B
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acids
- quantitative
- highly multiplexed
- multiplexed detection
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La presente invención se refiere a métodos de detección y cuantificación de ácidos nucleicos blanco de muestras en reacciones en una sola cámara multiplexada. Se proporcionan cámaras que incorporan consumibles optimizadas para reducir la señal de fondo próxima a matrices de alta eficiencia, así como métodos de uso. Dispositivos y sistemas configurados para utilizar los consumibles para la práctica de los métodos son una característica de la invención.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161561198P | 2011-11-17 | 2011-11-17 | |
PCT/US2012/025699 WO2012112925A2 (en) | 2011-02-18 | 2012-02-17 | Quantitative, highly multiplexed detection of nucleic acids |
US13/399,872 US20120214686A1 (en) | 2011-02-18 | 2012-02-17 | Quantitative, Highly Multiplexed Detection of Nucleic Acids |
PCT/US2012/051236 WO2013074163A1 (en) | 2011-11-17 | 2012-08-16 | Quantitative, highly multiplexed detection of nucleic acids |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2014005912A MX2014005912A (es) | 2014-09-04 |
MX338076B true MX338076B (es) | 2016-04-01 |
Family
ID=48430026
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2014005912A MX338076B (es) | 2011-11-17 | 2012-08-16 | Deteccion cuantitativa, altamente multiplexada de acidos nucleicos. |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP2780479A4 (es) |
JP (1) | JP2014533502A (es) |
KR (1) | KR20140094007A (es) |
CN (1) | CN104093857A (es) |
AU (1) | AU2012337389A1 (es) |
BR (1) | BR112014011937A2 (es) |
CA (1) | CA2855953A1 (es) |
HK (1) | HK1199746A1 (es) |
IL (1) | IL232586A0 (es) |
MX (1) | MX338076B (es) |
SG (1) | SG11201402341QA (es) |
WO (1) | WO2013074163A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6050555B2 (ja) * | 2013-07-15 | 2016-12-21 | シージーン アイエヌシー | Ptoの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出 |
WO2016168640A1 (en) | 2015-04-16 | 2016-10-20 | William Marsh Rice University | Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species |
CN110016500A (zh) * | 2018-01-10 | 2019-07-16 | 深圳闪量科技有限公司 | 表面探针定量pcr方法 |
AU2020388573A1 (en) * | 2019-11-21 | 2022-06-23 | 10X Genomics, Inc, | Spatial analysis of analytes |
CN111808989A (zh) * | 2020-06-18 | 2020-10-23 | 重庆浦洛通基因医学研究院有限公司 | 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法 |
JP2023545506A (ja) | 2020-10-15 | 2023-10-30 | ジェネンテック, インコーポレイテッド | 多重化された標的結合候補スクリーニング分析 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5422253A (en) * | 1992-12-07 | 1995-06-06 | Wisconsin Alumni Research Foundation | Method of site specific nucleic acid cleavage |
US6350580B1 (en) * | 2000-10-11 | 2002-02-26 | Stratagene | Methods for detection of a target nucleic acid using a probe comprising secondary structure |
WO2003042353A2 (en) * | 2001-07-17 | 2003-05-22 | Stratagene | Methods for detection of a target nucleic acid by capture using multi-subunit probes |
WO2005010199A2 (en) * | 2003-07-16 | 2005-02-03 | Geneohm Sciences, Inc. | Invasive cleavage reaction with electrochemical readout |
WO2005068660A1 (en) * | 2003-12-19 | 2005-07-28 | Beckman Coulter, Inc. | Solid-phase multiplexed invader assay |
CA2672246A1 (en) * | 2006-06-30 | 2008-03-13 | Rosetta Genomics Ltd | Detecting nucleic acids |
EP2118310B1 (en) * | 2006-12-29 | 2013-03-06 | Applied Biosystems, LLC | Systems and methods for detecting nucleic acids |
EP2077336A1 (en) * | 2007-12-19 | 2009-07-08 | Koninklijke Philips Electronics N.V. | Device and method for parallel quantitative analysis of multiple nucleic acids |
US20120040853A1 (en) * | 2008-11-21 | 2012-02-16 | Koninklijke Philips Electronics N.V. | Real time multiplex pcr detection on solid surfaces using double stranded nucleic acid specific dyes |
-
2012
- 2012-08-16 WO PCT/US2012/051236 patent/WO2013074163A1/en active Application Filing
- 2012-08-16 KR KR1020147016411A patent/KR20140094007A/ko not_active Application Discontinuation
- 2012-08-16 SG SG11201402341QA patent/SG11201402341QA/en unknown
- 2012-08-16 JP JP2014542300A patent/JP2014533502A/ja active Pending
- 2012-08-16 CA CA2855953A patent/CA2855953A1/en not_active Abandoned
- 2012-08-16 MX MX2014005912A patent/MX338076B/es active IP Right Grant
- 2012-08-16 AU AU2012337389A patent/AU2012337389A1/en not_active Abandoned
- 2012-08-16 EP EP12849697.3A patent/EP2780479A4/en not_active Withdrawn
- 2012-08-16 CN CN201280067273.7A patent/CN104093857A/zh active Pending
- 2012-08-16 BR BR112014011937A patent/BR112014011937A2/pt not_active IP Right Cessation
-
2014
- 2014-05-13 IL IL232586A patent/IL232586A0/en unknown
-
2015
- 2015-01-08 HK HK15100214.3A patent/HK1199746A1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
JP2014533502A (ja) | 2014-12-15 |
CN104093857A (zh) | 2014-10-08 |
BR112014011937A2 (pt) | 2017-05-30 |
HK1199746A1 (en) | 2015-07-17 |
EP2780479A4 (en) | 2015-04-08 |
SG11201402341QA (en) | 2014-06-27 |
EP2780479A1 (en) | 2014-09-24 |
KR20140094007A (ko) | 2014-07-29 |
IL232586A0 (en) | 2014-06-30 |
MX2014005912A (es) | 2014-09-04 |
AU2012337389A1 (en) | 2014-06-19 |
WO2013074163A1 (en) | 2013-05-23 |
CA2855953A1 (en) | 2013-05-23 |
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