MX367666B - Composiciones y métodos para cuantificar una secuencia de ácido nucleico en una muestra. - Google Patents
Composiciones y métodos para cuantificar una secuencia de ácido nucleico en una muestra.Info
- Publication number
- MX367666B MX367666B MX2016004384A MX2016004384A MX367666B MX 367666 B MX367666 B MX 367666B MX 2016004384 A MX2016004384 A MX 2016004384A MX 2016004384 A MX2016004384 A MX 2016004384A MX 367666 B MX367666 B MX 367666B
- Authority
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- Mexico
- Prior art keywords
- methods
- sample
- quantifying
- compositions
- nucleic acid
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
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- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
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- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a composiciones y métodos para cuantificar la detección de un oligonucleótido objetivo en una muestra en tiempo real. Estos métodos son compatibles con los oligonucleótidos objetivo amplificados al utilizar una reacción NEAR.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261621975P | 2012-04-09 | 2012-04-09 | |
| PCT/US2013/035750 WO2013155056A1 (en) | 2012-04-09 | 2013-04-09 | Compositions and methods for quantifying a nucleic acid sequence in a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX367666B true MX367666B (es) | 2019-08-30 |
Family
ID=49328090
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014012214A MX338296B (es) | 2012-04-09 | 2013-04-09 | Composiciones y metodos para cuantificar una secuencia de acido nucleico en una muestra. |
| MX2016004384A MX367666B (es) | 2012-04-09 | 2013-04-09 | Composiciones y métodos para cuantificar una secuencia de ácido nucleico en una muestra. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014012214A MX338296B (es) | 2012-04-09 | 2013-04-09 | Composiciones y metodos para cuantificar una secuencia de acido nucleico en una muestra. |
Country Status (18)
| Country | Link |
|---|---|
| US (8) | US9096897B2 (es) |
| EP (3) | EP2836609B2 (es) |
| JP (4) | JP5801513B2 (es) |
| KR (4) | KR102362649B1 (es) |
| CN (2) | CN104685066B (es) |
| AU (3) | AU2013246080C1 (es) |
| BR (1) | BR112014025262A8 (es) |
| CA (1) | CA2869971C (es) |
| DK (2) | DK2836609T4 (es) |
| ES (2) | ES2743050T5 (es) |
| FI (1) | FI3587587T3 (es) |
| IN (1) | IN2014DN08831A (es) |
| MX (2) | MX338296B (es) |
| NZ (2) | NZ723570A (es) |
| RU (2) | RU2732589C2 (es) |
| UA (2) | UA116205C2 (es) |
| WO (1) | WO2013155056A1 (es) |
| ZA (3) | ZA201408116B (es) |
Families Citing this family (47)
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| US12043866B2 (en) | 2010-08-13 | 2024-07-23 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| KR102362649B1 (ko) | 2012-04-09 | 2022-02-14 | 엔바이로로직스 인크. | 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법 |
| MX386087B (es) * | 2014-04-22 | 2025-03-18 | Envirologix Inc | Composiciones y metodos para mejorar y/o predecir la amplificacion de adn. |
| EP3137629A4 (en) * | 2014-04-30 | 2017-12-27 | Envirologix Inc. | Compositions and methods for detecting huanglongbing |
| JP6773651B2 (ja) * | 2014-10-14 | 2020-10-21 | アボット・ラボラトリーズAbbott Laboratories | ポリdnaスペーサー配列を有する配列変換およびシグナル増幅dnaならびにそれらを用いた検出方法 |
| US10793922B2 (en) * | 2014-10-20 | 2020-10-06 | Envirologix Inc. | Compositions and methods for detecting an RNA virus |
| EP3250709B1 (en) * | 2015-01-30 | 2019-12-25 | Envirologix Inc. | Compositions and methods for rapid detection of salmonella |
| US10570441B2 (en) * | 2015-01-30 | 2020-02-25 | Envirologix Inc. | Compositions and methods for detecting nicking enzyme and polymerase activity using a substrate molecule |
| CA3021580A1 (en) * | 2015-06-25 | 2016-12-29 | Barry L. Merriman | Biomolecular sensors and methods |
| US20200208199A1 (en) * | 2015-07-20 | 2020-07-02 | Envirologix Inc. | Compositions and methods for rapid detection of salmonella serovar d1 |
| US20180320228A1 (en) * | 2015-10-30 | 2018-11-08 | Alere Inc. | Determination of polymorphisms using isothermal nucleic acid amplification |
| US10329601B2 (en) | 2015-12-28 | 2019-06-25 | Ionian Technologies, Inc. | Nicking and extension amplification reaction (NEAR) of Streptococcus species |
| CN113985017A (zh) | 2016-01-14 | 2022-01-28 | 罗斯韦尔生物技术股份有限公司 | 分子传感器及相关方法 |
| JP7280590B2 (ja) | 2016-01-28 | 2023-05-24 | ロズウェル バイオテクノロジーズ,インコーポレイテッド | 大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置 |
| EP3408219B1 (en) | 2016-01-28 | 2022-08-17 | Roswell Biotechnologies, Inc | Massively parallel dna sequencing apparatus |
| KR20240170584A (ko) | 2016-02-09 | 2024-12-03 | 로스웰 엠이 아이엔씨. | 전자 비표지 dna 및 게놈 시퀀싱 |
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| KR102622275B1 (ko) | 2017-01-10 | 2024-01-05 | 로스웰 바이오테크놀로지스 인코포레이티드 | Dna 데이터 저장을 위한 방법들 및 시스템들 |
| CA3052140A1 (en) | 2017-01-19 | 2018-07-26 | Roswell Biotechnologies, Inc. | Solid state sequencing devices comprising two dimensional layer materials |
| US10508296B2 (en) | 2017-04-25 | 2019-12-17 | Roswell Biotechnologies, Inc. | Enzymatic circuits for molecular sensors |
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| WO2018218250A2 (en) * | 2017-05-26 | 2018-11-29 | Vibrant Holdings, Llc | Photoactive compounds and methods for biomolecule detection and sequencing |
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