DE60324810D1 - HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN - Google Patents

HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN

Info

Publication number
DE60324810D1
DE60324810D1 DE60324810T DE60324810T DE60324810D1 DE 60324810 D1 DE60324810 D1 DE 60324810D1 DE 60324810 T DE60324810 T DE 60324810T DE 60324810 T DE60324810 T DE 60324810T DE 60324810 D1 DE60324810 D1 DE 60324810D1
Authority
DE
Germany
Prior art keywords
helicase
sures
nuclear
dependent amplification
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60324810T
Other languages
English (en)
Inventor
Huimin Kong
Myriam Vincent
Yan Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32033597&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60324810(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of DE60324810D1 publication Critical patent/DE60324810D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Optical Recording Or Reproduction (AREA)
  • Glass Compositions (AREA)
DE60324810T 2002-09-20 2003-09-19 HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN Expired - Lifetime DE60324810D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41229802P 2002-09-20 2002-09-20
US44666203P 2003-02-11 2003-02-11
PCT/US2003/029020 WO2004027025A2 (en) 2002-09-20 2003-09-19 Helicase dependent amplification of nucleic acids

Publications (1)

Publication Number Publication Date
DE60324810D1 true DE60324810D1 (de) 2009-01-02

Family

ID=32033597

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60324810T Expired - Lifetime DE60324810D1 (de) 2002-09-20 2003-09-19 HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN

Country Status (8)

Country Link
US (3) US7282328B2 (de)
EP (1) EP1539979B1 (de)
JP (1) JP4632788B2 (de)
AT (1) ATE414792T1 (de)
AU (1) AU2003272438B2 (de)
CA (1) CA2498764C (de)
DE (1) DE60324810D1 (de)
WO (1) WO2004027025A2 (de)

Families Citing this family (277)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
AU2003215391B2 (en) * 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
WO2005118853A2 (en) * 2004-06-01 2005-12-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
EP2071038B1 (de) * 2003-04-25 2014-11-26 Becton Dickinson and Company Detektion der Herpes-simplex-Viren vom Typ 1 und 2 durch Nukleinsäureamplifizierung
US20050069926A1 (en) * 2003-08-01 2005-03-31 Affymetrix, Inc. Helicase-amplified reverse transcription
CN101208437B (zh) * 2003-12-12 2012-07-04 圣路易斯大学 检测大分子和其它分析物的生物传感器
WO2005089508A2 (en) * 2004-03-18 2005-09-29 Atom Sciences, Inc. Dna sequence detection by limited primer extension
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
EP1871527B1 (de) * 2004-12-23 2017-09-27 Abbott Point of Care Inc. Molekulares diagnostiksystem
WO2006074334A2 (en) 2005-01-05 2006-07-13 Biohelix Corporation Identification of rna targets using helicases
EP1841879A4 (de) * 2005-01-25 2009-05-27 Population Genetics Technologi Isothermische dna-amplifikation
EP1866434B1 (de) * 2005-02-19 2013-06-05 Avacta Group plc Isotherme nukleinsäureamplifikation
AU2006244617B2 (en) 2005-05-09 2013-04-18 Labrador Diagnostics Llc Point-of-care fluidic systems and uses thereof
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
CA2611198C (en) 2005-06-10 2015-04-28 Saint Louis University Methods for the selection of aptamers
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
US8062850B2 (en) 2005-07-25 2011-11-22 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
JP2009506788A (ja) * 2005-09-06 2009-02-19 ジェン−プローブ・インコーポレーテッド 核酸の等温増幅のための方法、組成物及びキット
US8703445B2 (en) 2005-12-29 2014-04-22 Abbott Point Of Care Inc. Molecular diagnostics amplification system and methods
US7368265B2 (en) 2006-01-23 2008-05-06 Compass Genetics, Llc Selective genome amplification
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US20100173364A1 (en) * 2006-04-11 2010-07-08 New England Biolabs, Inc. Repair of Nucleic Acids for Improved Amplification
WO2007120808A2 (en) * 2006-04-14 2007-10-25 Biohelix Corporation A method of asymmetric helicase-dependent amplification for probe-based detection of targets
AU2007298650B2 (en) 2006-05-04 2013-10-17 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
AU2007258455B2 (en) 2006-06-06 2013-05-02 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
US20090047673A1 (en) * 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8338109B2 (en) 2006-11-02 2012-12-25 Mayo Foundation For Medical Education And Research Predicting cancer outcome
EP2118280B1 (de) 2007-02-23 2010-12-01 New England Biolabs, Inc. Auswahl und anreicherung von proteinen durch in-vitro-untergliederung
US7838235B2 (en) * 2007-03-28 2010-11-23 Signal Diagnostics System and method for high resolution analysis of nucleic acids to detect sequence variations
CN101970111B (zh) 2007-06-21 2013-09-11 简·探针公司 用于执行处理的仪器和容器
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
MX2010003578A (es) 2007-10-02 2010-06-09 Theranos Inc Dispositivos modulares de punto de cuidado y usos de los mismos.
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
JP2011522521A (ja) 2008-05-05 2011-08-04 ロスアラモス ナショナル セキュリティ，エルエルシー 高度に単純化された側方流動ベースの核酸サンプル調製および受動的流体流動制御
CA2723726C (en) 2008-05-13 2017-09-12 Michael M. Becker Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
WO2009143603A1 (en) 2008-05-28 2009-12-03 Genomedx Biosciences, Inc. Systems and methods for expression-based discrimination of distinct clinical disease states in prostate cancer
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
CN102317779A (zh) 2008-11-21 2012-01-11 圣路易大学 检测目标物多个表位的生物感应器
AU2010208386B2 (en) * 2009-01-27 2016-08-11 Qiagen Gaithersburg Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
JP2010172248A (ja) * 2009-01-29 2010-08-12 National Institute Of Advanced Industrial Science & Technology 核酸の新規定量方法並びにそれに用いる新規試薬キット
JP5586631B2 (ja) 2009-01-30 2014-09-10 ジェン−プローブ・インコーポレーテッド 信号を検出し、信号伝送要素に熱エネルギーを加えるためのシステム及び方法
WO2010091111A1 (en) 2009-02-03 2010-08-12 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
WO2010129934A2 (en) 2009-05-07 2010-11-11 Veracyte, Inc. Methods and compositions for diagnosis of thyroid conditions
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
WO2010138187A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Scaffolded nucleic acid polymer particles and methods of making and using
EP2438196B1 (de) 2009-06-05 2016-12-21 Alere San Diego, Inc. Kits und reagenzien für rekombinase-polymerase-amplifikationen
CA2768391C (en) 2009-07-21 2016-09-06 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
JP6057460B2 (ja) 2009-08-31 2017-01-11 ジェン−プローブ・インコーポレーテッド デングウイルスアッセイ
US8951733B2 (en) 2009-10-16 2015-02-10 Monsanto Technology Llc Methods of polynucleotide detection
US9121054B2 (en) * 2009-12-08 2015-09-01 Biohelix Corporation Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
US20120288861A1 (en) 2009-12-21 2012-11-15 Heinz-Josef Lenz Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085160A1 (en) * 2010-01-08 2011-07-14 Qiagen Gaithersburg, Inc. Materials and methods for isothermal nucleic acid amplification
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
CA2787483C (en) 2010-02-12 2018-03-06 Saint Louis University Molecular biosensors capable of signal amplification
US20110318748A1 (en) 2010-02-26 2011-12-29 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
EP2556171B1 (de) 2010-04-05 2015-09-02 Prognosys Biosciences, Inc. Räumlich kodierte biologische assays
US8628914B2 (en) 2010-05-26 2014-01-14 Qiagen Gaithersburg, Inc. Quantitative helicase assay
US9822397B2 (en) 2010-06-30 2017-11-21 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
US9121053B2 (en) * 2010-07-08 2015-09-01 University Of Central Florida Research Foundation, Inc. Method of detecting single nucleotide polymorphisms
BR112013004044A2 (pt) 2010-08-13 2016-07-05 Envirologix Inc composições e métodos para quantificação de uma sequência de ácido nucleico em uma amostra.
EP2420579A1 (de) 2010-08-17 2012-02-22 QIAGEN GmbH Helikasenabhängige isothermale Amplifikation mit Nicking-Enzymen
JP6393478B2 (ja) 2010-10-12 2018-09-19 モンサント テクノロジー エルエルシー トランスジェニック事象ｍｏｎ８７７１２に対応するダイズ植物および種子、ならびにそれを検出するための方法
TWI690594B (zh) 2011-01-21 2020-04-11 美商賽瑞諾斯Ｉｐ有限責任公司 樣本使用最大化之系統及方法
AU2012239989B2 (en) 2011-04-07 2016-03-10 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
KR20140044323A (ko) 2011-04-20 2014-04-14 메사 테크 인터내셔널, 인코포레이티드 핵산의 왕복 증폭 반응
JP6223332B2 (ja) 2011-06-30 2017-11-01 モンサント テクノロジー エルエルシー 形質転換事象ｋｋ１７９−２に対応するアルファルファ植物および種子、ならびにその検出方法
EP2751264B1 (de) 2011-09-01 2017-12-27 New England Biolabs, Inc. Zusammensetzungen und verfahren im zusammenhang mit varianten dna-polymerasen und synthetischen dna polymerasen
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
AU2012312169B2 (en) 2011-09-21 2016-01-14 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
MX344792B (es) 2011-09-25 2017-01-06 Theranos Inc Sistemas y métodos para múltiples análisis.
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US9758823B2 (en) 2011-10-21 2017-09-12 Oxford Nanopore Technologies Limited Enzyme method
CA2858581A1 (en) 2011-12-13 2013-06-20 Genomedx Biosciences, Inc. Cancer diagnostics using non-coding transcripts
AU2012360244B2 (en) 2011-12-29 2018-08-23 Oxford Nanopore Technologies Limited Enzyme method
CA2881200A1 (en) * 2012-02-14 2013-08-22 Great Basin Scientific Methods of isothermal amplification using blocked primers
CA2864035C (en) 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
KR102048946B1 (ko) 2012-04-09 2019-11-27 엔바이로로직스 인크. 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
CA3096342C (en) 2012-06-08 2023-04-04 Ionian Technologies, Llc Nucleic acid amplifications
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
AU2013291765C1 (en) * 2012-07-19 2019-08-08 Oxford Nanopore Technologies Limited Enzyme construct
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
AU2013202793B2 (en) 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
DK3435084T3 (da) 2012-08-16 2023-05-30 Mayo Found Medical Education & Res Prostatakræftprognose under anvendelse af biomarkører
US8993298B1 (en) 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
KR20150055002A (ko) 2012-09-11 2015-05-20 테라노스, 인코포레이티드 생물학적 서명을 이용한 정보 관리 시스템 및 방법
EP2909337B1 (de) 2012-10-17 2019-01-09 Spatial Transcriptomics AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
CN102876813B (zh) * 2012-10-25 2013-11-13 中华人民共和国北京出入境检验检疫局 一种检测禽流感病毒的实时荧光rt-hda试剂盒及引物
CN102912038B (zh) * 2012-10-25 2014-05-28 中华人民共和国北京出入境检验检疫局 一种检测禽流感病毒的rt-hda试剂盒及引物
US9074248B1 (en) 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
US20150329900A1 (en) * 2012-12-18 2015-11-19 Qiagen Gmbh Nucleic Acid Amplification Method
AU2014216060A1 (en) 2013-02-18 2015-08-20 Theranos Ip Company, Llc Systems and methods for collecting and transmitting assay results
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN108872555B (zh) 2013-03-11 2021-04-09 克忧健康公司 用于检测和量化分析物的系统和方法
JP2016510991A (ja) 2013-03-11 2016-04-14 エリテックグループ・ベスローテン・フェンノートシャップＥｌｉｔｅｃｈｇｒｏｕｐ Ｂ．Ｖ． 正確な鎖置換型等温増幅のための方法
US10545161B2 (en) 2013-03-11 2020-01-28 Cue Health Inc. Systems and methods for detection and quantification of analytes
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US9623409B2 (en) 2013-03-11 2017-04-18 Cue Inc. Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
WO2014210416A1 (en) 2013-06-27 2014-12-31 New England Biolabs, Inc. Helicase suppression of non-template amplification
AU2014293075A1 (en) 2013-07-25 2015-12-17 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
US9315842B2 (en) * 2013-08-19 2016-04-19 Steven A Benner Helicase dependent amplification of DNA molecules using nucleotide analogs
MX2016004595A (es) 2013-10-09 2016-08-01 Monsanto Technology Llc Evento de maiz transgenico mon87403 y metodos para su deteccion.
ES2925313T3 (es) 2013-10-09 2022-10-14 Fluoresentric Inc Método para la detección de secuencias de ácido nucleico diana
EP3575410A3 (de) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modifizierte enzyme
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
MX367192B (es) 2013-10-30 2019-08-08 Green Life Biotech Llc Sistemas de cuantificacion de patogenesis y metodos de tratamiento para el enverdecimiento de los citricos.
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
SG11201604737VA (en) 2013-12-11 2016-07-28 Accuragen Inc Compositions and methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CA2946737A1 (en) 2014-04-22 2015-10-29 Envirologix, Inc. Compositions and methods for enhancing and/or predicting dna amplification
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
USD745423S1 (en) 2014-05-12 2015-12-15 Cue Inc. Automated analyzer test cartridge and sample collection device for analyte detection
WO2016011280A1 (en) 2014-07-16 2016-01-21 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
WO2016013620A1 (ja) * 2014-07-24 2016-01-28 学校法人関西学院 好熱性アーキア由来ＤＥＡＤ－ｂｏｘ型ＲＮＡへリカーゼ及びその改変体を用いるＤＮＡ増幅方法、並びにその方法のために使用する好熱性アーキア由来ＤＥＡＤ－ｂｏｘ型ＲＮＡへリカーゼ及びその改変体
US10274484B2 (en) 2014-09-12 2019-04-30 Mediomics Llc Molecular biosensors with a modular design
US10480026B2 (en) * 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
WO2016073353A1 (en) 2014-11-03 2016-05-12 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
JP7356788B2 (ja) 2014-11-05 2023-10-05 ベラサイト インコーポレイテッド 機械学習および高次元転写データを使用して経気管支生検における特発性肺線維症を診断するシステムおよび方法
MY182476A (en) 2014-11-11 2021-01-25 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP3218467A4 (de) 2014-11-13 2018-04-11 The Board of Trustees of the University of Illinois Biologisch manipulierte hyperfunktionale »super -helikasen
ES2870097T3 (es) 2014-12-15 2021-10-26 Illumina Inc Método de colocación molecular individual sobre un sustrato
MX2017009833A (es) 2015-01-30 2017-11-02 Envirologix Inc Molecula de sustrato.
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
EP3283512A4 (de) 2015-04-17 2018-10-03 Distributed Bio Inc Verfahren zur massenhumanisierung von nichthumanen antikörpern
TWI824991B (zh) 2015-04-24 2023-12-11 美商梅薩生物科技股份有限公司 用於檢測目標核酸的卡匣及控制通過在用於檢測目標核酸的卡匣中之腔室的流體之垂直流的方法
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
WO2016193695A1 (en) 2015-05-29 2016-12-08 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
US11015217B2 (en) 2015-05-29 2021-05-25 Roche Molecular Systems, Inc. Method of inactivating microbes by citraconic anhydride
EP3325153B1 (de) 2015-07-17 2020-03-18 Cue Health Inc. Kartusche zur probenanalyse
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
PL3256609T3 (pl) 2015-09-15 2022-05-16 Neogen Corporation Sposoby wykrywania listerii w próbce środowiskowej
AU2016334233B2 (en) 2015-10-09 2023-01-05 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
ES2786974T3 (es) 2016-04-07 2020-10-14 Illumina Inc Métodos y sistemas para la construcción de bibliotecas de ácidos nucleicos normalizadas
US11427866B2 (en) 2016-05-16 2022-08-30 Accuragen Holdings Limited Method of improved sequencing by strand identification
DK3464628T3 (en) 2016-06-06 2024-06-03 Redvault Biosciences Lp Target reporter constructs and uses thereof
CN117822128A (zh) 2016-07-22 2024-04-05 俄勒冈健康与科学大学 单细胞全基因组文库及制备其的组合索引方法
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
US11414708B2 (en) 2016-08-24 2022-08-16 Decipher Biosciences, Inc. Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN107058287B (zh) * 2017-01-16 2020-06-16 浙江大学 一种恒温扩增体系中生成单链产物的方法
CA3050984A1 (en) 2017-01-20 2018-07-26 Decipher Biosciences, Inc. Molecular subtyping, prognosis, and treatment of bladder cancer
WO2018140540A1 (en) 2017-01-25 2018-08-02 Cue Health Inc. Systems and methods for enhanced detection and quantification of analytes
CA3055925A1 (en) 2017-03-09 2018-09-13 Decipher Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
JP6448695B2 (ja) 2017-03-21 2019-01-09 株式会社東芝 Ｒｎａ増幅方法、ｒｎａ検出方法及びアッセイキット
CA3241796A1 (en) 2017-03-24 2018-09-27 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
EP3913053A1 (de) 2017-04-23 2021-11-24 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
SG11201909394PA (en) 2017-04-23 2019-11-28 Illumina Inc Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN111094584A (zh) 2017-04-23 2020-05-01 伊鲁米那股份有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
CA3059840C (en) 2017-04-23 2022-04-26 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
WO2018205035A1 (en) 2017-05-12 2018-11-15 Genomedx Biosciences, Inc Genetic signatures to predict prostate cancer metastasis and identify tumor agressiveness
EP3635136B1 (de) 2017-06-07 2021-10-20 Oregon Health & Science University Einzelzellen-ganzgenombibliotheken zur methylierungssequenzierung
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
EP4289507A3 (de) 2017-07-10 2024-02-28 Gen-Probe Incorporated Analytische systeme und verfahren zur nukleinsäure-amplifikation mit der verwendung von den proben zugeteilten parametern
US20200202977A1 (en) 2017-07-31 2020-06-25 Illumina, Inc. Sequencing system with multiplexed biological sample aggregation
CA3076516A1 (en) 2017-10-02 2019-04-11 Quidel Corporation Phage-based detection method for antimicrobial susceptibility testing and identification of bacterial species
WO2019118550A1 (en) 2017-12-13 2019-06-20 Gen-Probe Incorporated Methods for biological sample processing
JP2021506274A (ja) 2017-12-15 2021-02-22 ジェン−プローブ・インコーポレーテッド 毒素産生Ｃｌｏｓｔｒｉｄｉｕｍ ｄｉｆｆｉｃｉｌｅを検出するための組成物および方法
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
US20210071242A1 (en) 2018-01-29 2021-03-11 Gen-Probe Incorporated Analytical systems and methods
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
AU2019248635B2 (en) 2018-04-02 2022-01-27 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CN112941155B (zh) * 2018-05-14 2023-07-14 北京艾克伦医疗科技有限公司 具有茎环结构的dna引物对及其应用
MX2019015013A (es) 2018-05-17 2021-01-08 Illumina Inc Secuenciación de células individuales de alto rendimiento con sesgo de amplificación reducido.
CN111247248A (zh) 2018-06-04 2020-06-05 伊鲁米纳公司 高通量单细胞转录组文库及制备和使用方法
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
GB2597566B (en) 2018-06-13 2023-02-01 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
BR112020026306A2 (pt) * 2018-06-26 2021-03-30 The Broad Institute Inc. Métodos de amplificação, sistemas e diagnósticos baseados em sistema efeitor crispr
CA3105684A1 (en) 2018-07-10 2020-01-16 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
JP7469290B2 (ja) 2018-08-01 2024-04-16 ジェン－プローブ・インコーポレーテッド エプスタイン－バールウイルスの核酸を検出するための組成物および方法
AU2019319203A1 (en) 2018-08-08 2021-03-11 Gen-Probe Incorporated Compositions, methods and kits for detecting
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3856934A2 (de) 2018-09-27 2021-08-04 Gen-Probe Incorporated Zusammensetzungen und verfahren zur detektion von bordetella-pertussis- und bordetella-parapertussis-nukleinsäure
EP3894585A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Erzeugung von erfassungssonden zur räumlichen analyse
EP3899037B1 (de) 2018-12-19 2023-10-18 Illumina, Inc. Verfahren zur verbesserung der ponynucleotid-cluster-klonalitätspriorität
WO2020142347A2 (en) 2018-12-31 2020-07-09 Gen-Probe Incorporated Systems and methods for filling multi-well cartridges with solid reagents
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
SG11202102530QA (en) 2019-03-01 2021-04-29 Illumina Inc High-throughput single-nuclei and single-cell libraries and methods of making and of using
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
EP3938538A1 (de) 2019-03-15 2022-01-19 10X Genomics, Inc. Verfahren zur verwendung von räumlichen anordnungen für einzelzellsequenzierung
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
US20220098647A1 (en) 2019-03-22 2022-03-31 Gen-Probe Incorporated Compositions and Methods for Detecting Group A Streptococcus
CA3176696C (en) 2019-05-03 2024-06-11 Gen-Probe Incorporated Receptacle transport system for an analytical system
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
AU2020335996A1 (en) 2019-08-23 2022-03-31 Gen-Probe Incorporated Compositions, methods and kits for detecting Treponema pallidum
US20220333162A1 (en) 2019-09-05 2022-10-20 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021084637A1 (ja) 2019-10-30 2021-05-06 三菱電機株式会社 ソレノイド装置、およびスタータ
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2021102003A1 (en) 2019-11-18 2021-05-27 10X Genomics, Inc. Systems and methods for tissue classification
US20210155982A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc. Pipeline for spatial analysis of analytes
WO2021102005A1 (en) 2019-11-22 2021-05-27 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
CA3134746A1 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
SG11202106899SA (en) 2019-12-23 2021-09-29 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
IL296604A (en) 2020-03-30 2022-11-01 Illumina Inc Methods and preparations for preparing nucleic acid libraries
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
JP2023524117A (ja) 2020-04-30 2023-06-08 スタブ ヴィーダ － インヴェスティガサォン エ セルヴィソス エム シエンシアス バイオロジカス エルディーエー コロナウイルスの疑いのある試料中の核酸配列を検出するための方法及びポータブルデバイス
WO2021231477A2 (en) 2020-05-12 2021-11-18 Illumina, Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
WO2022006081A1 (en) 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
AU2021308095A1 (en) 2020-07-17 2023-03-09 Gen-Probe Incorporated Detection of macrolide-resistant mycoplasma genitalium
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CA3177256A1 (en) 2020-10-21 2022-04-28 Gen-Probe Incorporated Fluid container management system
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
CN117043352A (zh) 2021-01-29 2023-11-10 伊鲁米纳公司 使用多核苷酸改善流通池的接种效率的方法、组合物和试剂盒
JP2024510996A (ja) 2021-03-15 2024-03-12 ジェン－プローブ・インコーポレーテッド 生物学的サンプル処理のための組成物および方法
AU2022245985A1 (en) 2021-03-22 2023-09-21 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20220396778A1 (en) * 2021-06-12 2022-12-15 Shi-Lung Lin Novel RNA Composition and Production Method for Use in iPS Cell Generation
EP4382624A3 (de) 2021-07-27 2024-09-04 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von salmonella und mindestens einem von c. jejuni, c. coli, shigella und stec
US20240271186A1 (en) 2021-08-03 2024-08-15 Wageningen Universiteit Argonaute-based nucleic acid detection system
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20230279462A1 (en) * 2022-03-01 2023-09-07 Spectacular Labs, Inc. Methods for detecting microorganisms
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
EP4282980A1 (de) 2022-05-23 2023-11-29 Mobidiag Oy Methoden zum verstärken einer nukleinsäure
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5541099A (en) * 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DE69131321T2 (de) * 1990-09-28 1999-12-16 F. Hoffmann-La Roche Ag, Basel Mutationen in der 5'-3'-exonukleaseaktivität thermostabiler dna-polymerasen
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JPH05146299A (ja) * 1991-11-27 1993-06-15 Toyobo Co Ltd 核酸配列の増幅方法およびそのための試薬キツト
US6555349B1 (en) * 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
JPH0716094A (ja) * 1993-06-15 1995-01-20 Toyobo Co Ltd Ｒｎａポリメラーゼのプロモーター配列をもつ１本鎖オリゴヌクレオチドを製造する方法
AU687535B2 (en) * 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1999037820A1 (en) 1998-01-26 1999-07-29 Saigene Corporation An exponential nucleic acid amplification method using a single primer of opposing polarity
US6218122B1 (en) 1998-06-19 2001-04-17 Rosetta Inpharmatics, Inc. Methods of monitoring disease states and therapies using gene expression profiles
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
WO2000041524A2 (en) 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
WO2001025473A1 (en) 1999-06-28 2001-04-12 Source Precision Medicine, Inc. Systems and methods for characterizing a biological condition or agent using calibrated gene expression profiles
JP2003506048A (ja) * 1999-07-30 2003-02-18 ストラタジーン 古細菌複製補助因子及び使用方法
US7657379B2 (en) 2000-07-05 2010-02-02 Microsoft Corporation Methods and systems for determining the biological function of cell constituents using response profiles
AU2002307371B2 (en) * 2001-04-20 2008-04-03 Pennsylvania State University Methods for nucleic acid manipulation
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
AU2003215391B2 (en) * 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification

Also Published As

Publication number Publication date
US20070254304A1 (en) 2007-11-01
EP1539979A2 (de) 2005-06-15
JP2006500028A (ja) 2006-01-05
US7282328B2 (en) 2007-10-16
US20040058378A1 (en) 2004-03-25
WO2004027025A2 (en) 2004-04-01
EP1539979A4 (de) 2006-01-11
ATE414792T1 (de) 2008-12-15
JP4632788B2 (ja) 2011-02-16
AU2003272438A1 (en) 2004-04-08
AU2003272438B2 (en) 2009-04-02
EP1539979B1 (de) 2008-11-19
US7829284B2 (en) 2010-11-09
WO2004027025A3 (en) 2004-07-08
US20070207495A1 (en) 2007-09-06
CA2498764A1 (en) 2004-04-01
CA2498764C (en) 2015-11-10

Similar Documents

Publication Publication Date Title
DE60324810D1 (de) HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
HUP0302803A2 (hu) Nukleinsavak izotermikus amplifikációja szilárd hordozón
ATE329059T1 (de) Eine vollständig intern kontrollierte amplifikationsreaktion ermöglichende zusammensetzungen und verfahren
DE602004025801D1 (de) Dna-polymerasefusionen und deren verwendungen
ATE483194T1 (de) Verfahren und system zum auswählen und manipulieren von mehreren objekten
WO2004048931A3 (en) Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
ATE437945T1 (de) Verfahren zur amplifikation und sequenzierung von nukleinsäuren
WO2004065582A3 (en) Amplification of dna in a hairpin structure, and applications
DK1252328T3 (da) Fremgangsmåde og midler til isolering af mikroorganismer
TW200616578A (en) Fluid handling devices
EP0702090A3 (de) Verfahren zur Koamplifikation einer Zielnukleinsäure unter Versendung eines Volumenabscheidungsmittel in der Reaktionszusammensetzung, Testsatz und Testvorrichtung
MX2007012486A (es) Ensayos de investigacion sobre estilbazio.
WO2006030455A8 (en) Dna polymerases having strand displacement activity
ATE466080T1 (de) Formulierungen und verfahren zum denaturieren von proteinen
ATE401421T1 (de) Verfahren zum nachweis von dna methylierung
ATE439441T1 (de) Veränderte, fluoreszierende proteine
ATE522619T1 (de) Selektives kulturmedium zur isolation und/oder detektion von spezies des streptococcus genus
DK1465927T3 (da) BAG3-antistoffer til anvendelse til forskning, diagnostik og behandling af sygdomme, der omfatter celledød
ATE403663T1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
AU2002367750A8 (en) Isothermal amplification in nucleic acid analysis
ATE466103T1 (de) Polynukleotide zur amplifikation undzum nachweis von chlamydia trachomatis und neisseria gonorrhoeae
AU2003257177A8 (en) Improved compositions for in vitro amplification of nucleic acids
DE60108337D1 (de) Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion
DE60236996D1 (de) Kulturmedium zum nachweis von bakterien der gattung listeria
DE60324819D1 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus

Legal Events

Date Code Title Description
8363 Opposition against the patent