SG11202102530QA - High-throughput single-nuclei and single-cell libraries and methods of making and of using - Google Patents

High-throughput single-nuclei and single-cell libraries and methods of making and of using

Info

Publication number
SG11202102530QA
SG11202102530QA SG11202102530QA SG11202102530QA SG11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA SG 11202102530Q A SG11202102530Q A SG 11202102530QA
Authority
SG
Singapore
Prior art keywords
nuclei
making
methods
cell libraries
throughput
Prior art date
Application number
SG11202102530QA
Inventor
Sanjay Srivatsan
Jose Mcfaline-Figueroa
Vijay Ramani
Junyue Cao
Gregory Booth
Jay Shendure
Cole Trapnell
Frank J Steemers
Original Assignee
Illumina Inc
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc, Univ Washington filed Critical Illumina Inc
Publication of SG11202102530QA publication Critical patent/SG11202102530QA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
SG11202102530QA 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using SG11202102530QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962812853P 2019-03-01 2019-03-01
PCT/US2020/020637 WO2020180778A1 (en) 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using

Publications (1)

Publication Number Publication Date
SG11202102530QA true SG11202102530QA (en) 2021-04-29

Family

ID=70110324

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102530QA SG11202102530QA (en) 2019-03-01 2020-03-02 High-throughput single-nuclei and single-cell libraries and methods of making and of using

Country Status (12)

Country Link
US (1) US20220033805A1 (en)
EP (1) EP3837365A1 (en)
JP (2) JP2022522917A (en)
KR (1) KR20210134598A (en)
CN (1) CN113302300A (en)
AU (1) AU2020232618A1 (en)
BR (1) BR112021006234A2 (en)
CA (1) CA3113841A1 (en)
IL (1) IL281829A (en)
MX (1) MX2021003746A (en)
SG (1) SG11202102530QA (en)
WO (1) WO2020180778A1 (en)

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CN114107459B (en) * 2021-11-11 2022-12-06 浙江大学 High-throughput single cell sequencing method based on oligonucleotide chain hybridization marker
CN116694730A (en) * 2022-02-28 2023-09-05 南方科技大学 Construction method of single cell open chromatin and transcriptome co-sequencing library
IL292281A (en) 2022-04-14 2023-11-01 Yeda Res & Dev Methods of single cell rna-sequencing
CN117497037B (en) * 2023-11-17 2024-08-16 上海倍谙基生物科技有限公司 Culture medium component sensitivity analysis method based on generalized linear model

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Also Published As

Publication number Publication date
JP2023062105A (en) 2023-05-02
IL281829A (en) 2021-05-31
CN113302300A (en) 2021-08-24
WO2020180778A9 (en) 2021-04-08
AU2020232618A1 (en) 2021-04-08
US20220033805A1 (en) 2022-02-03
BR112021006234A2 (en) 2021-09-28
MX2021003746A (en) 2021-06-23
WO2020180778A1 (en) 2020-09-10
JP2022522917A (en) 2022-04-21
CA3113841A1 (en) 2020-09-10
KR20210134598A (en) 2021-11-10
EP3837365A1 (en) 2021-06-23

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