BR112016025349A2 - composições e métodos para a detecção de huanglongbing - Google Patents

composições e métodos para a detecção de huanglongbing

Info

Publication number
BR112016025349A2
BR112016025349A2 BR112016025349A BR112016025349A BR112016025349A2 BR 112016025349 A2 BR112016025349 A2 BR 112016025349A2 BR 112016025349 A BR112016025349 A BR 112016025349A BR 112016025349 A BR112016025349 A BR 112016025349A BR 112016025349 A2 BR112016025349 A2 BR 112016025349A2
Authority
BR
Brazil
Prior art keywords
huanglongbing
extract
detecting
methods
citrus
Prior art date
Application number
BR112016025349A
Other languages
English (en)
Inventor
Parker Breck
Lampton Paula
TAPLEY Susan
Original Assignee
Envirologix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Envirologix Inc filed Critical Envirologix Inc
Publication of BR112016025349A2 publication Critical patent/BR112016025349A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

a presente invenção apresenta composições e métodos para a detecção de huanglongbing (hlb) em árvores de citrinos e insetos. em um aspecto, a invenção proporciona um método para detectar uma infecção de huanglongbing (hlb) em uma plantação de citros envolvendo a obtenção de um extrato a partir de uma amostra biológica obtida a partir de uma plantação de citros, contatar o extrato com primers diretos e inversos que se ligam especificamente a uma molécula de ácido nucleico de candidutus na presença de uma enzima nicking, dntps e uma polimerase sob condições permissivas para a amplificação isotérmica da molécula de ácido nucleico; e a detecção de um amplicón de candidutus no extrato.
BR112016025349A 2014-04-30 2015-04-28 composições e métodos para a detecção de huanglongbing BR112016025349A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461986587P 2014-04-30 2014-04-30
PCT/US2015/028006 WO2015168134A1 (en) 2014-04-30 2015-04-28 Compositions and methods for detecting huanglongbing

Publications (1)

Publication Number Publication Date
BR112016025349A2 true BR112016025349A2 (pt) 2017-12-12

Family

ID=54359243

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016025349A BR112016025349A2 (pt) 2014-04-30 2015-04-28 composições e métodos para a detecção de huanglongbing

Country Status (8)

Country Link
US (1) US10100370B2 (pt)
EP (1) EP3137629A4 (pt)
JP (1) JP2017516466A (pt)
CN (1) CN106574299A (pt)
BR (1) BR112016025349A2 (pt)
MX (1) MX2016014292A (pt)
WO (1) WO2015168134A1 (pt)
ZA (1) ZA201607571B (pt)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109517924A (zh) * 2019-01-17 2019-03-26 四川省农业科学院园艺研究所 一种柑橘花青苷积累形成红肉性状的分子检测方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ701145A (en) 2012-04-09 2016-09-30 Envirologix Inc Compositions and methods for quantifying a nucleic acid sequence in a sample
AR100157A1 (es) 2014-04-22 2016-09-14 Envirologix Inc Composiciones y métodos para mejorar y/o predecir la amplificación de adn
US20190137476A1 (en) * 2016-03-25 2019-05-09 The Regents Of The University Of California Detecting huanglongbing (hlb) in citrus plants by analyzing changes in emitted volatile organic compounds
CN107354224A (zh) * 2017-08-31 2017-11-17 中国检验检疫科学研究院 一种用于检测柑桔黄龙病菌的引物探针组合及检测方法
CN108424976A (zh) * 2018-02-08 2018-08-21 杭州富集生物科技有限公司 突发急性烈性传染病应急检测储备试剂盒及检验方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100404690C (zh) * 2005-12-29 2008-07-23 重庆大学 柑桔黄龙病菌实时荧光pcr检测引物、探针、固相化试剂盒及其检测方法
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
NZ701145A (en) * 2012-04-09 2016-09-30 Envirologix Inc Compositions and methods for quantifying a nucleic acid sequence in a sample
CN102605092B (zh) 2012-04-09 2013-06-12 中国农业科学院柑桔研究所 柑橘黄龙病的lamp快速检测方法
CN103589794A (zh) * 2013-11-04 2014-02-19 中国热带农业科学院环境与植物保护研究所 一种实时荧光恒温定量检测柑橘黄龙病的方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109517924A (zh) * 2019-01-17 2019-03-26 四川省农业科学院园艺研究所 一种柑橘花青苷积累形成红肉性状的分子检测方法

Also Published As

Publication number Publication date
EP3137629A1 (en) 2017-03-08
EP3137629A4 (en) 2017-12-27
US10100370B2 (en) 2018-10-16
MX2016014292A (es) 2017-02-13
ZA201607571B (en) 2018-05-30
US20170114394A1 (en) 2017-04-27
CN106574299A (zh) 2017-04-19
JP2017516466A (ja) 2017-06-22
WO2015168134A1 (en) 2015-11-05

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]