BR112018013539A2 - métodos de preparar capsinoides por processos biossintéticos - Google Patents
métodos de preparar capsinoides por processos biossintéticosInfo
- Publication number
- BR112018013539A2 BR112018013539A2 BR112018013539A BR112018013539A BR112018013539A2 BR 112018013539 A2 BR112018013539 A2 BR 112018013539A2 BR 112018013539 A BR112018013539 A BR 112018013539A BR 112018013539 A BR112018013539 A BR 112018013539A BR 112018013539 A2 BR112018013539 A2 BR 112018013539A2
- Authority
- BR
- Brazil
- Prior art keywords
- capsinoids
- preparing
- methods
- biosynthetic processes
- cell mixture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/52—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation
- C07C67/54—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation by distillation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/56—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/58—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/60—Separation; Purification; Stabilisation; Use of additives by treatment giving rise to chemical modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
- C07C69/52—Esters of acyclic unsaturated carboxylic acids having the esterified carboxyl group bound to an acyclic carbon atom
- C07C69/587—Monocarboxylic acid esters having at least two carbon-to-carbon double bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
a presente invenção refere-se a métodos de preparar capsinoides incluindo fornecer um capsiato sintase em uma mistura ou sistema celular, alimentando 8-metil-6-nonenoil-coa, ácido 6e-8-metilnonenóico ou ácido 8-metilnonanóico na mistura ou sistema celular, alimentando álcool vanilílico na mistura ou sistema celular, e coletando capsinoides da mistura ou sistema celular.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662276059P | 2016-01-07 | 2016-01-07 | |
PCT/US2017/012535 WO2017120473A1 (en) | 2016-01-07 | 2017-01-06 | Methods of making capsinoids by biosynthetic processes |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018013539A2 true BR112018013539A2 (pt) | 2018-12-04 |
Family
ID=59274312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018013539A BR112018013539A2 (pt) | 2016-01-07 | 2017-01-06 | métodos de preparar capsinoides por processos biossintéticos |
Country Status (10)
Country | Link |
---|---|
US (1) | US10655150B2 (pt) |
EP (1) | EP3377057A4 (pt) |
JP (1) | JP2019500877A (pt) |
KR (1) | KR20180101445A (pt) |
CN (1) | CN108472279A (pt) |
AU (1) | AU2017205501A1 (pt) |
BR (1) | BR112018013539A2 (pt) |
CA (1) | CA3010716A1 (pt) |
MX (1) | MX2018008351A (pt) |
WO (1) | WO2017120473A1 (pt) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20190027830A (ko) | 2016-07-19 | 2019-03-15 | 코나겐 인크. | 특정 자연 캡사이시노이드의 미생물 생산 방법 |
CN111187161B (zh) * | 2020-01-20 | 2021-10-01 | 中国科学技术大学 | 一种二氢辣椒素和二氢辣椒素酯的制备方法 |
CN115960736B (zh) * | 2023-03-01 | 2024-05-28 | 石河子大学 | 一种产香草胺和辣椒碱的酿酒酵母工程菌及其构建方法与应用 |
CN116837016B (zh) * | 2023-08-29 | 2024-01-02 | 中国农业科学院北京畜牧兽医研究所 | 一种构建生产辣椒素香草壬酰胺的重组大肠杆菌工程菌株的方法及重组菌株和应用 |
Family Cites Families (31)
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US5958745A (en) | 1996-03-13 | 1999-09-28 | Monsanto Company | Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants |
JP4043605B2 (ja) | 1998-07-02 | 2008-02-06 | 丸善製薬株式会社 | カプサイシン類縁体の製造法 |
US20070244192A1 (en) | 1999-01-14 | 2007-10-18 | Martek Biosciences Corporation | Plant seed oils containing polyunsaturated fatty acids |
DE10000978A1 (de) | 2000-01-12 | 2001-07-26 | Gvs Ges Fuer Erwerb Und Verwer | Verfahren zur Erhöhung des Gehalts an Fettsäuren in Pflanzen und Mikroorganismen |
US20040010817A1 (en) | 2000-07-21 | 2004-01-15 | Washington State University Research Foundation | Plant acyl-CoA synthetases |
JP3951008B2 (ja) | 2002-01-17 | 2007-08-01 | 国立大学法人 岡山大学 | カプサイシン分解合成酵素及びその生産方法 |
WO2003087834A2 (en) | 2002-04-08 | 2003-10-23 | Affinium Pharmaceuticals, Inc. | High throughput analysis of recombinant proteins in multi-well plates |
GB0421937D0 (en) | 2004-10-02 | 2004-11-03 | Univ York | Acyl CoA synthetases |
KR101132152B1 (ko) | 2005-02-18 | 2012-04-03 | 아지노모토 가부시키가이샤 | 탈수 축합반응에 의한 캡시노이드의 제조법, 캡시노이드의안정화 방법 및 캡시노이드 조성물 |
WO2006100680A2 (en) | 2005-03-22 | 2006-09-28 | Hazera Genetics Ltd. | Pepper plants having fruit with altered vitamin content |
JPWO2007021020A1 (ja) | 2005-08-16 | 2009-02-26 | 味の素株式会社 | カプシノイド類含有組成物 |
JP2009529891A (ja) | 2006-03-15 | 2009-08-27 | マーテック バイオサイエンシーズ コーポレーション | 多価不飽和脂肪酸を含む植物種子油 |
EP2090662A3 (en) | 2006-04-05 | 2012-10-31 | Metanomics GmbH | Process for the production of a fine chemical |
ES2307420B1 (es) | 2007-04-24 | 2009-10-20 | Universidad De Cadiz | Procedimiento de sintesis quimica de capsinoides. |
AU2008335089B2 (en) | 2007-12-11 | 2014-06-26 | Synthetic Genomics, Inc. | Secretion of fatty acids by photosynthetic microorganisms |
BRPI0914419A2 (pt) | 2008-06-23 | 2015-08-11 | Ajinomoto Kk | Planta geneticamente modificada, e, métodos para produzir a mesma, e para produzir capsinóide |
CA2851316C (en) | 2011-07-13 | 2021-10-19 | National Research Council Of Canada | Genes and proteins for alkanoyl-coa synthesis |
US8951762B2 (en) | 2011-07-27 | 2015-02-10 | Iowa State University Research Foundation, Inc. | Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis |
KR101983115B1 (ko) | 2011-08-08 | 2019-05-29 | 에볼바 에스아 | 사프란 화합물의 재조합 생성을 위한 방법 및 물질 |
AU2012327162A1 (en) | 2011-12-27 | 2013-07-11 | Commonwealth Scientific And Industrial Research Organisation | Production of dihydrosterculic acid and derivatives thereof |
KR20150036502A (ko) | 2012-07-13 | 2015-04-07 | 칼리스타, 인코포레이티드 | 바이오리파이너리 시스템, 이의 방법 및 조성물 |
CN103710315B (zh) | 2012-10-30 | 2016-03-23 | 浙江工业大学 | 来自冬虫夏草的长链-酰基-辅酶a合成酶、基因及应用 |
US10184140B2 (en) | 2013-01-23 | 2019-01-22 | Iowa State University Research Foundation, Inc. | Materials and methods for production of bi-functional fatty acids in recombinant bacteria |
CN103173444A (zh) | 2013-03-27 | 2013-06-26 | 广东省农业科学院蔬菜研究所 | 一种准确区分辣椒与甜椒的功能标记及其检测引物组和检测方法 |
CA2962266A1 (en) | 2013-11-01 | 2015-05-07 | Conagen Inc. | Methods of using acyl-coa synthetase for biosynthetic production of acyl-coas |
CN103725652B (zh) | 2013-12-25 | 2016-06-29 | 无锡新和源发酵技术研究院有限公司 | 一种酰基辅酶a合成酶及其应用 |
MX2016009328A (es) * | 2014-01-17 | 2017-04-27 | Conagen Inc | Metodos para usar capsaicina sintasa para la produccion microbiana de capsaicinoides. |
WO2015160842A1 (en) * | 2014-04-14 | 2015-10-22 | Flex Pharma, Inc. | Methods and formulatiions of capsaicinoids and capsinoids |
EP3020819A1 (en) | 2014-11-17 | 2016-05-18 | Evonik Degussa GmbH | Fatty acid and derivatives production |
KR20190027830A (ko) | 2016-07-19 | 2019-03-15 | 코나겐 인크. | 특정 자연 캡사이시노이드의 미생물 생산 방법 |
JP6986084B2 (ja) | 2016-12-22 | 2021-12-22 | コナジェン・インコーポレイテッドConagen Inc. | 8−メチルノナン酸の微生物生成方法 |
-
2017
- 2017-01-06 EP EP17736436.1A patent/EP3377057A4/en not_active Withdrawn
- 2017-01-06 AU AU2017205501A patent/AU2017205501A1/en not_active Abandoned
- 2017-01-06 BR BR112018013539A patent/BR112018013539A2/pt not_active Application Discontinuation
- 2017-01-06 MX MX2018008351A patent/MX2018008351A/es unknown
- 2017-01-06 CA CA3010716A patent/CA3010716A1/en not_active Abandoned
- 2017-01-06 CN CN201780005728.5A patent/CN108472279A/zh active Pending
- 2017-01-06 US US16/067,161 patent/US10655150B2/en active Active
- 2017-01-06 WO PCT/US2017/012535 patent/WO2017120473A1/en active Application Filing
- 2017-01-06 JP JP2018535130A patent/JP2019500877A/ja active Pending
- 2017-01-06 KR KR1020187022312A patent/KR20180101445A/ko unknown
Also Published As
Publication number | Publication date |
---|---|
KR20180101445A (ko) | 2018-09-12 |
AU2017205501A1 (en) | 2018-07-05 |
WO2017120473A1 (en) | 2017-07-13 |
US20190010522A1 (en) | 2019-01-10 |
EP3377057A1 (en) | 2018-09-26 |
US10655150B2 (en) | 2020-05-19 |
EP3377057A4 (en) | 2018-12-05 |
CA3010716A1 (en) | 2017-07-13 |
CN108472279A (zh) | 2018-08-31 |
MX2018008351A (es) | 2018-12-06 |
JP2019500877A (ja) | 2019-01-17 |
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Legal Events
Date | Code | Title | Description |
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B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |