BR112018075548A2 - método para estabilizar proteínas - Google Patents

método para estabilizar proteínas

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Publication number
BR112018075548A2
BR112018075548A2 BR112018075548-6A BR112018075548A BR112018075548A2 BR 112018075548 A2 BR112018075548 A2 BR 112018075548A2 BR 112018075548 A BR112018075548 A BR 112018075548A BR 112018075548 A2 BR112018075548 A2 BR 112018075548A2
Authority
BR
Brazil
Prior art keywords
product
stabilize proteins
cells expressing
culturing
recovering
Prior art date
Application number
BR112018075548-6A
Other languages
English (en)
Inventor
Khan Mohsam
Porter Alison
Original Assignee
Lonza Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ltd filed Critical Lonza Ltd
Publication of BR112018075548A2 publication Critical patent/BR112018075548A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/28—Means for regulation, monitoring, measurement or control, e.g. flow regulation of redox potential
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/20—Heating or cooling
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Thermal Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

a presente revelação apresenta métodos e composições para aumentar a quantidade de produtos de metabolismo celular, por exemplo, proteínas, ao diminuir a temperatura de células que expressam o produto em uma ou mais etapas enquanto se cultiva as células, que expressam o produto, e/ou recuperando o produto.
BR112018075548-6A 2016-06-10 2017-06-12 método para estabilizar proteínas BR112018075548A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662348595P 2016-06-10 2016-06-10
US62/348,595 2016-06-10
PCT/EP2017/064313 WO2017212071A1 (en) 2016-06-10 2017-06-12 Method for stabilizing proteins

Publications (1)

Publication Number Publication Date
BR112018075548A2 true BR112018075548A2 (pt) 2019-04-09

Family

ID=59253468

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018075548-6A BR112018075548A2 (pt) 2016-06-10 2017-06-12 método para estabilizar proteínas

Country Status (13)

Country Link
US (2) US10975409B2 (pt)
EP (1) EP3469091A1 (pt)
JP (2) JP2019517272A (pt)
KR (2) KR102451374B1 (pt)
CN (1) CN109496237A (pt)
AU (1) AU2017277761B2 (pt)
BR (1) BR112018075548A2 (pt)
CA (1) CA3026589A1 (pt)
EA (1) EA201892820A1 (pt)
IL (1) IL263342B2 (pt)
MX (1) MX2018015298A (pt)
SG (1) SG11201810975UA (pt)
WO (1) WO2017212071A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017277761B2 (en) 2016-06-10 2021-11-11 Lonza Ltd Method for stabilizing proteins
WO2018175688A1 (en) * 2017-03-22 2018-09-27 Bonti, Inc. Botulinum neurotoxins for use in therapy
US11760777B2 (en) * 2017-04-26 2023-09-19 Bristol-Myers Squibb Company Methods of antibody production that minimize disulfide bond reduction
US10814338B2 (en) * 2017-08-09 2020-10-27 Delta Separations, Llc Device, system and methods for separation and purification of organic compounds from botanical material
AU2018395215A1 (en) * 2017-12-28 2020-08-13 Locus Ip Company, Llc Reactors and submerged fermentation methods for producing microbe-based products
EP3875595A4 (en) * 2018-11-02 2022-07-13 Kyowa Kirin Co., Ltd. METHOD FOR PREPARING LIQUID CULTURE MEDIUM
CN109932328B (zh) * 2019-03-27 2021-06-25 西京学院 一种速溶咖啡中丙烯酰胺含量的可见分光光度测定方法
US20230323422A1 (en) * 2020-08-14 2023-10-12 Bristol-Myers Squibb Company Method of making protein

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Publication number Priority date Publication date Assignee Title
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
IT1258959B (it) 1992-06-09 1996-03-11 Impianto a moduli mobili per lo sviluppo e la produzione di prodotti biotecnologici su scala pilota
CA2293632C (en) 1997-06-12 2011-11-29 Research Corporation Technologies, Inc. Artificial antibody polypeptides
WO2003072796A1 (fr) * 2002-02-28 2003-09-04 Yaeta Endo Solution de reaction pour la synthese de proteines sans cellule, procede de preparation de cette solution et procede de synthese de cette proteine utilisant cette solution
US8298054B2 (en) 2004-02-03 2012-10-30 Xcellerex, Inc. System and method for manufacturing
GB0410118D0 (en) 2004-05-06 2004-06-09 Glaxo Group Ltd Novel bioreactor
MXPA06014099A (es) 2004-06-04 2007-05-09 Xcellerex Inc Sistemas biorreactores desechables y metodos.
WO2007067656A2 (en) 2005-12-05 2007-06-14 Hope Ernest G Prevalidated, modular good manufacturing practice-compliant facility
JP2010524467A (ja) 2007-04-16 2010-07-22 モメンタ ファーマシューティカルズ インコーポレイテッド 規定の糖タンパク質産物および関連の方法
HUE037633T2 (hu) * 2007-07-09 2018-09-28 Genentech Inc Diszulfidkötés redukciójának megelõzése polipeptidek rekombináns elõállítása során
EP2216395A1 (en) * 2009-02-09 2010-08-11 Lonza Biologics plc. Bioreactor for the cultivation of mammalian cells
US8771635B2 (en) 2010-04-26 2014-07-08 Toyota Motor Engineering & Manufacturing North America, Inc. Hydrogen release from complex metal hydrides by solvation in ionic liquids
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WO2012122413A1 (en) 2011-03-08 2012-09-13 University Of Maryland Baltimore County Microscale bioprocessing system and method for protein manufacturing
DK2763694T3 (en) * 2011-10-04 2018-04-16 Statens Seruminstitut Preparation of a cysteine-rich protein
EP3674320A3 (en) * 2011-10-27 2020-08-12 Genmab A/S Production of heterodimeric proteins
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JP6063362B2 (ja) * 2013-09-10 2017-01-18 株式会社日立製作所 光合成微生物の分離システム及びその分離方法
AU2017277761B2 (en) 2016-06-10 2021-11-11 Lonza Ltd Method for stabilizing proteins

Also Published As

Publication number Publication date
EP3469091A1 (en) 2019-04-17
CN109496237A (zh) 2019-03-19
IL263342A (en) 2018-12-31
KR102451374B1 (ko) 2022-10-11
US20200248223A1 (en) 2020-08-06
IL263342B1 (en) 2023-07-01
KR102499718B1 (ko) 2023-02-13
SG11201810975UA (en) 2019-01-30
JP2022191246A (ja) 2022-12-27
JP2019517272A (ja) 2019-06-24
US10975409B2 (en) 2021-04-13
US11993802B2 (en) 2024-05-28
US20170356022A1 (en) 2017-12-14
EA201892820A1 (ru) 2019-10-31
AU2017277761B2 (en) 2021-11-11
KR20190017885A (ko) 2019-02-20
IL263342B2 (en) 2023-11-01
KR20220139432A (ko) 2022-10-14
CA3026589A1 (en) 2017-12-14
WO2017212071A9 (en) 2018-04-12
AU2017277761A1 (en) 2018-12-20
MX2018015298A (es) 2019-04-11
WO2017212071A1 (en) 2017-12-14

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