MX2016016898A - Metodos y composiciones para analisis de muestras. - Google Patents
Metodos y composiciones para analisis de muestras.Info
- Publication number
- MX2016016898A MX2016016898A MX2016016898A MX2016016898A MX2016016898A MX 2016016898 A MX2016016898 A MX 2016016898A MX 2016016898 A MX2016016898 A MX 2016016898A MX 2016016898 A MX2016016898 A MX 2016016898A MX 2016016898 A MX2016016898 A MX 2016016898A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- compositions
- present
- sample
- sample analysis
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Abstract
La presente descripción se refiere a métodos y sistemas para el procesamiento y el análisis de muestras cuando la cantidad total de muestra de entrada es baja o cuando una diana de interés está presente como una población relativamente menor o poco frecuente dentro de la muestra general. La descripción se refiere particularmente al análisis de muestras de ácido nucleico, incluso muestras en donde un ácido nucleico de interés está presente como una proporción relativamente baja de los ácidos nucleicos generales.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462017580P | 2014-06-26 | 2014-06-26 | |
US201462063870P | 2014-10-14 | 2014-10-14 | |
PCT/US2015/038143 WO2015200871A1 (en) | 2014-06-26 | 2015-06-26 | Methods and compositions for sample analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2016016898A true MX2016016898A (es) | 2017-04-25 |
Family
ID=54929861
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016016898A MX2016016898A (es) | 2014-06-26 | 2015-06-26 | Metodos y composiciones para analisis de muestras. |
Country Status (10)
Country | Link |
---|---|
US (2) | US20150376605A1 (es) |
EP (1) | EP3161161A4 (es) |
JP (1) | JP2017523774A (es) |
KR (1) | KR20170023011A (es) |
CN (1) | CN106574298A (es) |
AU (1) | AU2015279619A1 (es) |
CA (1) | CA2953473A1 (es) |
IL (1) | IL249618A0 (es) |
MX (1) | MX2016016898A (es) |
WO (1) | WO2015200871A1 (es) |
Families Citing this family (71)
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US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
CN113528634A (zh) | 2012-08-14 | 2021-10-22 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2900543C (en) | 2013-02-08 | 2023-01-31 | 10X Genomics, Inc. | Partitioning and processing of analytes and other species |
US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
CN106413896B (zh) | 2014-04-10 | 2019-07-05 | 10X基因组学有限公司 | 用于封装和分割试剂的流体装置、系统和方法及其应用 |
CN114214314A (zh) | 2014-06-24 | 2022-03-22 | 生物辐射实验室股份有限公司 | 数字式pcr条码化 |
JP2017526046A (ja) | 2014-06-26 | 2017-09-07 | 10エックス ゲノミクス,インコーポレイテッド | 核酸配列アセンブルのプロセス及びシステム |
EP3161160B1 (en) | 2014-06-26 | 2021-10-13 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
CN114807307A (zh) | 2014-10-29 | 2022-07-29 | 10X 基因组学有限公司 | 用于靶核酸测序的方法和组合物 |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
CA2968417A1 (en) | 2015-01-13 | 2016-07-21 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
EP3256606B1 (en) | 2015-02-09 | 2019-05-22 | 10X Genomics, Inc. | Systems and methods for determining structural variation |
EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
JP2018505688A (ja) | 2015-02-24 | 2018-03-01 | 10エックス ゲノミクス,インコーポレイテッド | 標的化核酸配列包括度(coverage)のための方法 |
US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
US10774370B2 (en) | 2015-12-04 | 2020-09-15 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
EP3414341A4 (en) | 2016-02-11 | 2019-10-09 | 10X Genomics, Inc. | SYSTEMS, METHODS, AND MEDIA FOR ASSEMBLING NOVO OF GENOME SEQUENCE DATA OVERALL |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP4310183A3 (en) | 2017-01-30 | 2024-02-21 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
WO2018200867A1 (en) | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
WO2018213643A1 (en) | 2017-05-18 | 2018-11-22 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
CN110870018A (zh) | 2017-05-19 | 2020-03-06 | 10X基因组学有限公司 | 用于分析数据集的系统和方法 |
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SG11201901822QA (en) | 2017-05-26 | 2019-03-28 | 10X Genomics Inc | Single cell analysis of transposase accessible chromatin |
KR101979834B1 (ko) * | 2017-08-11 | 2019-05-17 | 한국과학기술원 | 미세유체장치를 이용한 다중유전자 디지털 신호 분석장치 및 이의 분석방법 |
US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
US10748643B2 (en) | 2017-08-31 | 2020-08-18 | 10X Genomics, Inc. | Systems and methods for determining the integrity of test strings with respect to a ground truth string |
US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
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EP3568494B1 (en) * | 2017-12-08 | 2021-05-05 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
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KR101913735B1 (ko) * | 2018-05-03 | 2018-11-01 | 주식회사 셀레믹스 | 차세대 염기서열 분석을 위한 시료 간 교차 오염 탐색용 내부 검정 물질 |
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US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
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EP3002339B1 (en) * | 2006-02-02 | 2019-05-08 | The Board of Trustees of The Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
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US20120252015A1 (en) * | 2011-02-18 | 2012-10-04 | Bio-Rad Laboratories | Methods and compositions for detecting genetic material |
US9625454B2 (en) * | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
KR101963462B1 (ko) * | 2010-10-04 | 2019-03-28 | 제납시스 인크. | 재사용 가능한 자동화 평행 생물 반응 시스템 및 방법 |
EP3736281A1 (en) * | 2011-02-18 | 2020-11-11 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
WO2012149042A2 (en) * | 2011-04-25 | 2012-11-01 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
US9469874B2 (en) * | 2011-10-18 | 2016-10-18 | The Regents Of The University Of California | Long-range barcode labeling-sequencing |
WO2013126741A1 (en) * | 2012-02-24 | 2013-08-29 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
CN113528634A (zh) * | 2012-08-14 | 2021-10-22 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
CA2890441A1 (en) * | 2012-11-07 | 2014-05-15 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
EP3567116A1 (en) * | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
KR102436171B1 (ko) * | 2013-06-27 | 2022-08-24 | 10엑스 제노믹스, 인크. | 샘플 처리를 위한 조성물 및 방법 |
-
2015
- 2015-06-26 JP JP2016575081A patent/JP2017523774A/ja active Pending
- 2015-06-26 EP EP15812045.1A patent/EP3161161A4/en not_active Withdrawn
- 2015-06-26 CA CA2953473A patent/CA2953473A1/en not_active Abandoned
- 2015-06-26 US US14/752,602 patent/US20150376605A1/en not_active Abandoned
- 2015-06-26 AU AU2015279619A patent/AU2015279619A1/en not_active Abandoned
- 2015-06-26 CN CN201580034146.0A patent/CN106574298A/zh active Pending
- 2015-06-26 MX MX2016016898A patent/MX2016016898A/es unknown
- 2015-06-26 KR KR1020167036360A patent/KR20170023011A/ko unknown
- 2015-06-26 WO PCT/US2015/038143 patent/WO2015200871A1/en active Application Filing
-
2016
- 2016-12-18 IL IL249618A patent/IL249618A0/en unknown
-
2019
- 2019-12-23 US US16/725,673 patent/US20200399631A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
IL249618A0 (en) | 2017-02-28 |
EP3161161A4 (en) | 2018-02-28 |
KR20170023011A (ko) | 2017-03-02 |
AU2015279619A1 (en) | 2017-01-12 |
US20200399631A1 (en) | 2020-12-24 |
CA2953473A1 (en) | 2015-12-30 |
US20150376605A1 (en) | 2015-12-31 |
JP2017523774A (ja) | 2017-08-24 |
WO2015200871A1 (en) | 2015-12-30 |
CN106574298A (zh) | 2017-04-19 |
EP3161161A1 (en) | 2017-05-03 |
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