MX2016016902A - Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas. - Google Patents

Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.

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Publication number
MX2016016902A
MX2016016902A MX2016016902A MX2016016902A MX2016016902A MX 2016016902 A MX2016016902 A MX 2016016902A MX 2016016902 A MX2016016902 A MX 2016016902A MX 2016016902 A MX2016016902 A MX 2016016902A MX 2016016902 A MX2016016902 A MX 2016016902A
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Mexico
Prior art keywords
cell
individual cells
cell populations
methods
nucleic acids
Prior art date
Application number
MX2016016902A
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English (en)
Inventor
Serge Saxonov
Schnall-Levin Michael
Jarosz Mirna
BHARADWAJ Rajiv
Dean Ness Kevin
J Hindson Benjamin
Martin Hindson Christopher
Hardenbol Paul
X Y Zheng Grace
Belgrader Philip
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of MX2016016902A publication Critical patent/MX2016016902A/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
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    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/16—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support involving encoding steps
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Chemical & Material Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

Métodos, composiciones y sistemas para analizar células individuales o poblaciones de células a través del análisis dividido de los contenidos de las células individuales o poblaciones de células. Las células individuales o poblaciones de células se codividen con reactivos de procesamiento para acceder a los contenidos celulares, y para identificar especialmente los contenidos de una determinada célula o población de células y, posteriormente, analizar los contenidos de la célula y caracterizarlo como derivado de una célula individual o población de células, incluso el análisis y la caracterización de los ácidos nucleicos de la célula a través de secuenciación.
MX2016016902A 2014-06-26 2015-06-26 Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas. MX2016016902A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462017558P 2014-06-26 2014-06-26
US201462061567P 2014-10-08 2014-10-08
PCT/US2015/038178 WO2015200893A2 (en) 2014-06-26 2015-06-26 Methods of analyzing nucleic acids from individual cells or cell populations

Publications (1)

Publication Number Publication Date
MX2016016902A true MX2016016902A (es) 2017-03-27

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MX2016016902A MX2016016902A (es) 2014-06-26 2015-06-26 Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.

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US (5) US20150376609A1 (es)
EP (4) EP3161160B1 (es)
JP (3) JP6838969B2 (es)
KR (2) KR102531677B1 (es)
CN (3) CN118910216A (es)
AU (1) AU2015279548B2 (es)
CA (1) CA2953374A1 (es)
IL (1) IL249617A0 (es)
MX (1) MX2016016902A (es)
WO (1) WO2015200893A2 (es)

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