SG10201806890VA - Massively parallel single cell analysis - Google Patents

Massively parallel single cell analysis

Info

Publication number
SG10201806890VA
SG10201806890VA SG10201806890VA SG10201806890VA SG10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA
Authority
SG
Singapore
Prior art keywords
cells
single cell
massively parallel
parallel single
compositions
Prior art date
Application number
SG10201806890VA
Inventor
Christina Fan
Stephen Fodor
Glenn Fu
Geoffrey Facer
Julie Wilhelmy
Original Assignee
Cellular Res Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
Priority to US201361871232P priority Critical
Priority to US201461952036P priority
Priority to US201462012237P priority
Application filed by Cellular Res Inc filed Critical Cellular Res Inc
Publication of SG10201806890VA publication Critical patent/SG10201806890VA/en
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51519182&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10201806890V(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Abstract

MASSIVELY PARALLEL SINGLE CELL ANALYSIS The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes). Fig. 1
SG10201806890VA 2013-08-28 2014-08-28 Massively parallel single cell analysis SG10201806890VA (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US201361871232P true 2013-08-28 2013-08-28
US201461952036P true 2014-03-12 2014-03-12
US201462012237P true 2014-06-13 2014-06-13

Publications (1)

Publication Number Publication Date
SG10201806890VA true SG10201806890VA (en) 2018-09-27

Family

ID=51519182

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201806890VA SG10201806890VA (en) 2013-08-28 2014-08-28 Massively parallel single cell analysis
SG11201601188TA SG11201601188TA (en) 2013-08-28 2014-08-28 Massively parallel single cell analysis

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201601188TA SG11201601188TA (en) 2013-08-28 2014-08-28 Massively parallel single cell analysis

Country Status (10)

Country Link
US (11) US9567645B2 (en)
EP (2) EP3039158B1 (en)
JP (1) JP2019106998A (en)
KR (1) KR20160055168A (en)
CN (1) CN105705659A (en)
DK (1) DK3039158T3 (en)
ES (1) ES2711168T3 (en)
GB (2) GB2525104B (en)
SG (2) SG10201806890VA (en)
WO (1) WO2015031691A1 (en)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2013019491A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) * 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) * 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2954104A4 (en) 2013-02-08 2016-08-24 10X Genomics Inc Polynucleotide barcode generation
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
KR20160055168A (en) 2013-08-28 2016-05-17 셀룰러 리서치, 인크. Massively parallel single cell analysis
CN105745528A (en) 2013-10-07 2016-07-06 赛卢拉研究公司 Methods and systems for digitally counting features on arrays
CA2929596A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CA2943624A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN108350497A (en) * 2015-08-28 2018-07-31 Illumina公司 Nucleic acid sequence analysis from single cells
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3177740A4 (en) 2014-08-06 2018-02-28 Nugen Technologies, Inc. Digital measurements from targeted sequencing
EP3212807A1 (en) 2014-10-29 2017-09-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US20160163235A1 (en) * 2014-12-09 2016-06-09 Avery Dennison Corporation Can End Label
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using the same
EP3259371A1 (en) 2015-02-19 2017-12-27 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
EP3262189A1 (en) * 2015-02-27 2018-01-03 Cellular Research, Inc. Methods and compositions for barcoding nucleic acids for sequencing
CN107208158A (en) 2015-02-27 2017-09-26 赛卢拉研究公司 Spatially addressable molecular barcoding
WO2016145416A2 (en) * 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
US20180073073A1 (en) * 2015-03-18 2018-03-15 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
JP2018509915A (en) * 2015-03-30 2018-04-12 セルラー リサーチ, インコーポレイテッド Methods and compositions for combinatorial barcoding
CN107810277A (en) * 2015-04-21 2018-03-16 通用自动化实验技术公司 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
US20160312276A1 (en) * 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3303633A1 (en) * 2015-05-26 2018-04-11 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
CN108350488A (en) * 2015-08-17 2018-07-31 加利福尼亚大学董事会 Microdroplet-based multiple displacement amplification (MDA) methods and related compositions
US20180340939A1 (en) * 2015-10-28 2018-11-29 The Broad Institute, Inc. Multiplex analysis of single cell constituents
CN108473984A (en) * 2015-11-04 2018-08-31 阿特雷卡公司 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
KR101745590B1 (en) * 2015-11-13 2017-06-09 고려대학교 산학협력단 Optical Microscopy for RNA Splicing on Single Molecules Using Scattering Intensity, Localized Surface Plasmon Resonance and Surface-Enhanced Raman Scattering
WO2017096110A1 (en) * 2015-12-04 2017-06-08 Siemens Healthcare Diagnostics Inc. Method and device for optimizing the process of identification of pathogens
WO2017124101A2 (en) * 2016-01-15 2017-07-20 The Broad Institute Inc. Semi-permeable arrays for analyzing biological systems and methods of using same
WO2017139501A1 (en) * 2016-02-10 2017-08-17 The Board Of Trustees Of The Leland Stanford Junior University Rna fixation and detection in clarity-based hydrogel tissue
US20190094224A1 (en) * 2016-04-11 2019-03-28 Board Of Regents, The University Of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
WO2018057820A1 (en) * 2016-09-21 2018-03-29 Predicine, Inc. Systems and methods for combined detection of genetic alterations
US20170314067A1 (en) * 2016-05-02 2017-11-02 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN107058310A (en) * 2016-08-12 2017-08-18 艾吉泰康生物科技(北京)有限公司 Amplicon library construction method with function of improving low-frequency mutation detection sensitivity of gene
CN109791157A (en) 2016-09-26 2019-05-21 赛卢拉研究公司 Use the reagent measuring protein expression with bar coded oligonucleotide sequence
WO2018064385A1 (en) * 2016-09-28 2018-04-05 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
US20180137242A1 (en) 2016-11-08 2018-05-17 Cellular Research, Inc. Methods for expression profile classification
US20180127744A1 (en) 2016-11-08 2018-05-10 Cellular Research, Inc. Methods for cell label classification
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR101903255B1 (en) * 2016-12-29 2018-10-02 한국표준과학연구원 Sensor for detecting botulinum neurotoxin using spinning carbon-nanotube
WO2018129222A1 (en) * 2017-01-04 2018-07-12 Carlos Genty A multi-well device for the processing, testing, and multiplexed analysis of intact, fixed, paraffin or plastic embedded (ifpe) biological materials
US20180200710A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
WO2018176007A1 (en) * 2017-03-23 2018-09-27 BioSpyder Technologies, Inc. Modulation of targets in cellular pathways identified by resolution of stochastic gene expression
US20180276332A1 (en) 2017-03-24 2018-09-27 Cellular Research, Inc. Synthetic multiplets for multiplets determination
WO2018174827A1 (en) * 2017-03-24 2018-09-27 National University Of Singapore Methods for multiplex detection of molecules
WO2018203141A1 (en) 2017-05-05 2018-11-08 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US20180356331A1 (en) 2017-06-12 2018-12-13 Becton, Dickinson And Company Index sorting systems and methods
WO2019018128A1 (en) 2017-07-18 2019-01-24 Becton, Dickinson And Company Dynamic interactive display of multi-parameter quantitative biological data
US20190026926A1 (en) 2017-07-18 2019-01-24 Becton, Dickinson And Company Dynamic interactive display of multi-parameter quantitative biological data
WO2019060804A1 (en) 2017-09-25 2019-03-28 Cellular Research, Inc. Immune receptor-barcode error correction
WO2019099908A1 (en) * 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019104337A1 (en) * 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides

Family Cites Families (357)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
US4510244A (en) 1980-04-17 1985-04-09 The Board Of Trustees Of The Leland Stanford Jr. University Cell labeling with antigen-coupled microspheres
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4725536A (en) 1985-09-19 1988-02-16 Genetics Institute, Inc. Reagent polynucleotide complex with multiple target binding regions, and kit and methods
US6150517A (en) 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Method and kits for the amplification and detection of nucleic acid sequences
IE72468B1 (en) 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5656731A (en) 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
JP2650159B2 (en) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー Nucleic acid amplification method
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
JPH04501353A (en) 1988-07-26 1992-03-12
RU2107729C1 (en) 1988-12-16 1998-03-27 Акцо Нобел Н.В. Amplification method
IE66597B1 (en) 1989-05-10 1996-01-24 Akzo Nv Method for the synthesis of ribonucleic acid (RNA)
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5925525A (en) 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DE69128545D1 (en) 1990-08-24 1998-02-05 Univ Tennessee Res Corp Technique of DNA fingerprinting with dns duplication
WO1992007095A1 (en) 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
DE69132843D1 (en) 1990-12-06 2002-01-10 Affymetrix Inc Identifying nucleic acids in samples
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
DE69233087D1 (en) 1991-11-22 2003-07-03 Affymetrix Inc N D Ges D Staat A process for the production of polymer arrays
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
DE69431719T2 (en) 1993-06-25 2003-09-18 Affymetrix Inc N D Ges D Staat Hybridization and sequencing of nucleic acids
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5500356A (en) 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
WO1995030774A1 (en) 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6600996B2 (en) 1994-10-21 2003-07-29 Affymetrix, Inc. Computer-aided techniques for analyzing biological sequences
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
ES2271944T3 (en) 1994-10-28 2007-04-16 Gen-Probe Incorporated Compositions and methods for the detection and quantitation of multiple simultaneous specific nucleic acid sequences.
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
CA2216645A1 (en) 1995-04-25 1996-11-21 Irori Remotely programmable matrices with memories and uses thereof
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US6147205A (en) 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US5981956A (en) 1996-05-16 1999-11-09 Affymetrix, Inc. Systems and methods for detection of labeled materials
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
JPH1021373A (en) 1996-07-05 1998-01-23 Fuji Photo Film Co Ltd Picture analyzing device
WO1998015644A2 (en) 1996-09-27 1998-04-16 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6117631A (en) 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
WO1998053300A2 (en) 1997-05-23 1998-11-26 Lynx Therapeutics, Inc. System and apparaus for sequential processing of analytes
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
JP2001521753A (en) 1997-10-31 2001-11-13 アフィメトリックス インコーポレイテッド Expression profiles of adult organs and fetal organs
AU1603199A (en) 1997-12-03 1999-06-16 Curagen Corporation Methods and devices for measuring differential gene expression
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6653077B1 (en) 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US6262216B1 (en) 1998-10-13 2001-07-17 Affymetrix, Inc. Functionalized silicon compounds and methods for their synthesis and use
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6197554B1 (en) 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US6629040B1 (en) 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
JP2002539849A (en) 1999-03-26 2002-11-26 アフィメトリックス インコーポレイテッド Universal array
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
CA2304260C (en) 1999-04-20 2009-03-24 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
US20030207300A1 (en) 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6326148B1 (en) 1999-07-12 2001-12-04 The Regents Of The University Of California Detection of copy number changes in colon cancer
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6436675B1 (en) * 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
SE9903988D0 (en) 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of Analysis
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
AT492652T (en) 2000-02-07 2011-01-15 Illumina Inc Nucleic acid detection methods with universal priming
US20020072058A1 (en) 2000-03-24 2002-06-13 Voelker Leroy L. Method for amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
US6613520B2 (en) 2000-04-10 2003-09-02 Matthew Ashby Methods for the survey and genetic analysis of populations
EP1368460B1 (en) 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Real-time sequence determination
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
AU2002246612B2 (en) 2000-10-24 2007-11-01 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic DNA
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
US20030049616A1 (en) 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
WO2002070684A2 (en) 2001-01-11 2002-09-12 Lion Bioscience Ag Gene library for screening methods
CA2435551C (en) 2001-01-25 2011-10-25 Tm Bioscience Corporation Families of non-cross-hybridizing polynucleotides for use as tags and tag complements, manufacture and use thereof
US7027932B2 (en) 2001-03-21 2006-04-11 Olympus Optical Co., Ltd. Biochemical examination method
JP2005233974A (en) 2001-03-21 2005-09-02 Olympus Corp Biochemical inspection method
AUPR480901A0 (en) 2001-05-04 2001-05-31 Genomics Research Partners Pty Ltd Diagnostic method for assessing a condition of a performance animal
CA2344599C (en) 2001-05-07 2011-07-12 Bioneer Corporation Selective polymerase chain reaction of dna of which base sequence is completely unknown
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
JP4244534B2 (en) 2001-06-12 2009-03-25 横河電機株式会社 Biochip
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US6830931B2 (en) 2001-07-12 2004-12-14 Automated Cell, Inc. Method and apparatus for monitoring of proteins and cells
EP1451365A4 (en) 2001-11-13 2006-09-13 Rubicon Genomics Inc Dna amplification and sequencing using dna molecules generated by random fragmentation
AU2003206095A1 (en) 2002-01-29 2003-09-02 Global Genomics Ab Methods and means for amplifying nucleic acid
US20070178478A1 (en) 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US20030186251A1 (en) 2002-04-01 2003-10-02 Brookhaven Science Associates, Llc Genome sequence tags
WO2004003219A2 (en) 2002-06-28 2004-01-08 Igen International, Inc. Improved assay systems and components
US20050019776A1 (en) 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
EP1546721B2 (en) 2002-08-16 2017-05-17 Avantra Biosciences Corporation Substrates for isolating, reacting and microscopically analyzing materials
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
WO2004031408A1 (en) 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
EP1578994A2 (en) 2002-11-11 2005-09-28 Affymetrix, Inc. Methods for identifying dna copy number changes
US7822555B2 (en) 2002-11-11 2010-10-26 Affymetrix, Inc. Methods for identifying DNA copy number changes
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
US7192700B2 (en) 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1861512A4 (en) 2005-03-18 2009-12-09 Fluidigm Corp Thermal reaction device and method for using the same
US7269518B2 (en) 2003-04-30 2007-09-11 Agilent Technologies, Inc. Chemical array reading
US20040259118A1 (en) 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
GB0319332D0 (en) 2003-08-16 2003-09-17 Astrazeneca Ab Amplification
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
AT461292T (en) 2003-09-10 2010-04-15 Althea Technologies Inc Expression profiling using microarrays
WO2005047521A2 (en) 2003-11-10 2005-05-26 Investigen, Inc. Methods of preparing nucleic acid for detection
JP2007524410A (en) 2004-01-23 2007-08-30 リングヴィテ エーエス Improvement of the polynucleotide ligation reaction
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
DE602005018166D1 (en) 2004-02-12 2010-01-21 Population Genetics Technologi sort genetic analysis using sequence-specific
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
WO2005111242A2 (en) 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
EP1771249A4 (en) 2004-06-07 2010-02-24 Irm Llc Dispensing systems, software, and related methods
US20060035258A1 (en) 2004-08-06 2006-02-16 Affymetrix, Inc. Methods for identifying DNA copy number changes
US20060041385A1 (en) 2004-08-18 2006-02-23 Bauer Kenneth D Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization
US7713697B2 (en) 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
US20060073506A1 (en) 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
ES2352041T5 (en) 2004-11-03 2014-12-22 Iris International, Inc. homogeneous detection of analytes
WO2006071770A2 (en) 2004-12-23 2006-07-06 I-Stat Corporation Molecular diagnostics system and methods
JP5420174B2 (en) 2004-12-23 2014-02-19 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション rna amplification method by ligation
EP1845160A4 (en) 2005-02-10 2009-07-01 Riken Method for amplification of nucleotide sequence
EP1856293A2 (en) 2005-03-16 2007-11-21 Compass Genetics, Llc Methods and compositions for assay readouts on multiple analytical platforms
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
US7695886B2 (en) 2005-05-19 2010-04-13 Fuji Xerox Co., Ltd. Process for producing resin particle liquid dispersion for electrostatic image developing toner, electrostatic image developing toner and production process thereof
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US7796815B2 (en) 2005-06-10 2010-09-14 The Cleveland Clinic Foundation Image analysis of biological objects
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
EP2485052B1 (en) 2005-09-13 2015-05-06 Affymetrix, Inc. Encoded microparticles
US20070117121A1 (en) 2005-09-16 2007-05-24 Hutchison Stephen K cDNA library preparation
US8313711B2 (en) 2005-11-01 2012-11-20 Freeslate, Inc. Liquid dispensing for high-throughput experimentation
US7375211B2 (en) 2005-11-18 2008-05-20 Kou Zhong C Method for detection and quantification of T-cell receptor Vβ repertoire
US20080070303A1 (en) * 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
US7636465B2 (en) 2005-12-09 2009-12-22 Cytyc Corporation Cross-frame object reconstruction for image-based cytology applications
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20080038727A1 (en) 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
EP2002246A1 (en) 2006-04-04 2008-12-17 Sidec Technologies AB Extended electron tomography
US20080194414A1 (en) 2006-04-24 2008-08-14 Albert Thomas J Enrichment and sequence analysis of genomic regions
WO2007136874A2 (en) 2006-05-18 2007-11-29 President And Fellows Of Harvard College Genomic library construction
CA2655272C (en) 2006-06-14 2017-04-18 Living Microsystems, Inc. Rare cell analysis using sample splitting and dna tags
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
EP2057465A4 (en) 2006-08-09 2010-04-21 Homestead Clinical Corp Organ-specific proteins and methods of their use
US20100159590A1 (en) 2006-10-05 2010-06-24 Nanopoint, Inc. Systems and methods for active microfluidic cell handling
WO2008057163A2 (en) 2006-10-09 2008-05-15 Carnegie Mellon University Preparation of functional gel particles with a dual crosslink network
ES2490601T3 (en) 2006-11-15 2014-09-04 Biospherex Llc multi-tag sequencing and analysis ecogenómico
US8050476B2 (en) 2006-11-22 2011-11-01 General Electric Company Heart rate demodulation of periodic movement in ultrasound data for object identification
CA2672315A1 (en) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
JP5489469B2 (en) 2007-01-16 2014-05-14 オリンパス株式会社 Fluorescent signal analyzer and fluorescence signal analysis method
US9063133B2 (en) 2007-01-30 2015-06-23 The Regents Of The University Of California Methods and devices for biomolecular arrays
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
WO2008096318A2 (en) 2007-02-09 2008-08-14 Koninklijke Philips Electronics N.V. Identification system
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
KR100882711B1 (en) 2007-03-12 2009-02-06 성균관대학교산학협력단 Uracil-DNA glycosylase of Psychrobacter sp. HJ147 and use thereof
JP2008256428A (en) 2007-04-03 2008-10-23 Intec Systems Institute Inc Method for detecting block position of microarray image
US20080274458A1 (en) 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US20090061513A1 (en) 2007-05-15 2009-03-05 Picovitro Ab Cell sorting and cell cultivation methods
EP2164985A4 (en) 2007-06-01 2014-05-14 454 Life Sciences Corp System and meth0d for identification of individual samples from a multiplex mixture
EP2173509A4 (en) 2007-06-25 2013-11-06 Affymetrix Inc Patterned microcodes
WO2009031054A2 (en) 2007-06-29 2009-03-12 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
EP2036989B1 (en) 2007-09-12 2012-07-25 Institut Pasteur Polynucleotide suitable for single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution
US20090253586A1 (en) 2008-02-21 2009-10-08 Gentel Biosciences, Inc. Substrates for multiplexed assays and uses thereof
US8687189B2 (en) 2008-03-03 2014-04-01 Ajjer, Llc Analysis of arrays by laser induced breakdown spectroscopy
DE102008025656B4 (en) 2008-05-28 2016-07-28 Genxpro Gmbh A method for quantitative analysis of nucleic acids, and their use for marker
US20090298709A1 (en) 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
WO2009148560A2 (en) 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Methods and compositions for nucleic acid sequencing
US8330957B2 (en) 2009-06-29 2012-12-11 Hager Enviromental and Atmospheric Technologies, LLC Device and method for quantification of gases in plumes by remote sensing
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
WO2010059820A1 (en) 2008-11-21 2010-05-27 Saint Louis University Biosensor for detecting multiple epitopes on a target
US20100159533A1 (en) 2008-11-24 2010-06-24 Helicos Biosciences Corporation Simplified sample preparation for rna analysis
US8492096B2 (en) 2008-12-24 2013-07-23 Boris Pasche TGFBR1 expression modifies risk for colorectal cancer
EP2389453B1 (en) 2009-01-20 2015-11-25 The Board of Trustees of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
BRPI1006113A2 (en) 2009-03-05 2016-02-16 Daiichi Sankyo Co Ltd "Pyridine derivative."
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
EP3002337B1 (en) 2009-03-30 2018-10-24 Illumina, Inc. Gene expression analysis in single cells
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
FR2945545B1 (en) 2009-05-14 2011-08-05 Univ Aix Marseille Ii prokaryotic DNA detection method to extract a stool sample
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
EP2449104B1 (en) 2009-06-29 2014-06-04 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
ES2562159T3 (en) 2009-08-20 2016-03-02 Population Genetics Technologies Ltd. Compositions and methods for the reordering of molecular nucleic acid
CN107419002A (en) 2009-09-01 2017-12-01 皇家飞利浦电子股份有限公司 Devices and methods for microarray selection
US8936762B2 (en) * 2009-09-01 2015-01-20 Trustees Of Boston University High throughput multichannel reader and uses thereof
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
WO2011041308A1 (en) 2009-09-30 2011-04-07 Quest Diagnostics Investments Incorporated Bcr-abl truncation mutations
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
AU2011207544A1 (en) 2010-01-19 2012-09-06 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic DNA by whole genome sequencing
AU2010343279B2 (en) 2010-01-19 2015-04-23 Verinata Health, Inc. Sequencing methods and compositions for prenatal diagnoses
EP3392349A1 (en) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digital analyte analysis
JP5901046B2 (en) 2010-02-19 2016-04-06 国立大学法人 千葉大学 Novel alternative splicing variant of OATP1B3mRNA
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
ES2697974T3 (en) 2010-06-09 2019-01-30 Keygene Nv Combinatorial sequence bar codes for high performance selection
EP2407242A1 (en) * 2010-07-13 2012-01-18 Dublin City University Direct clone analysis and selection technology
US20120040843A1 (en) 2010-08-12 2012-02-16 Dublin City University Centrifugal capture system
CA2811185A1 (en) 2010-09-21 2012-03-29 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
EP2436766A1 (en) 2010-09-29 2012-04-04 Deutsches Krebsforschungszentrum Means and methods for improved protein interaction screening
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
US20120088691A1 (en) 2010-10-08 2012-04-12 Gao Chen Highly multiplexed real-time pcr using encoded microbeads
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
CN103649713B (en) 2010-11-29 2017-03-01 丹麦达科有限公司 Sample image analysis method and system by a programmable quantitative assay of
US9394511B2 (en) * 2010-12-05 2016-07-19 Wenbin Jiang Rapid single cell based parallel biological cell sorter
CN107159328A (en) 2010-12-09 2017-09-15 阿科尼生物系统公司 Sample analysis system
EP2652155B1 (en) * 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
KR20120110431A (en) 2011-03-29 2012-10-10 에스케이하이닉스 주식회사 A semiconductor memory apparatus
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012142213A2 (en) 2011-04-15 2012-10-18 The Johns Hopkins University Safe sequencing system
CA2834291A1 (en) 2011-04-25 2012-11-01 Biorad Laboratories, Inc. Methods and compositions for nucleic acid analysis
GB201108259D0 (en) 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
EP2710172B1 (en) 2011-05-20 2017-03-29 Fluidigm Corporation Nucleic acid encoding reactions
CN103608033B (en) 2011-05-24 2016-06-15 生物技术公司 Individualized vaccine for cancer
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US20140147860A1 (en) 2011-06-27 2014-05-29 Life Technologies Corporation Acoustic Cytometry Methods and Protocols
WO2013019075A2 (en) 2011-08-01 2013-02-07 연세대학교산학협력단 Method of preparing nucleic acid molecules
JP5816486B2 (en) 2011-08-18 2015-11-18 オリンパス株式会社 Fluorescent image processing method of the fluorescence observation apparatus and fluoroscopy system and the fluorescence observation device
EP3467500A1 (en) 2011-11-11 2019-04-10 Eli N. Glezer Co-binder assisted assay methods
KR101337094B1 (en) 2011-11-30 2013-12-05 삼성에스디에스 주식회사 Apparatus and method for sequence alignment
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP3495817A1 (en) 2012-02-10 2019-06-12 Raindance Technologies, Inc. Molecular diagnostic screening assay
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
EP2820174A4 (en) 2012-02-27 2016-01-13 Univ North Carolina Methods and uses for molecular tags
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
UA115783C2 (en) 2012-05-08 2017-12-26 Адаптів Біотекнолоджіс Корпорейшн Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
CA2873585A1 (en) 2012-05-14 2013-11-21 Cb Biotechnologies, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
US9840732B2 (en) 2012-05-21 2017-12-12 Fluidigm Corporation Single-particle analysis of particle populations
CN108587867A (en) * 2012-05-25 2018-09-28 北卡罗来纳-查佩尔山大学 Microfluidic devices, solid supports for reagents and related methods
JP2015519909A (en) 2012-06-15 2015-07-16 アダプティブ バイオテクノロジーズ コーポレイション Specific tag appended reconstructed in complex gene set adaptive immune receptor gene
JP2015519911A (en) * 2012-06-15 2015-07-16 ボード・オブ・リージエンツ,ザ・ユニバーシテイ・オブ・テキサス・システム High throughput sequencing of a plurality transcripts
JP6181751B2 (en) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ, インコーポレイテッド Compositions and methods for the negative selection of unwanted nucleic sequence
JP6285929B2 (en) 2012-07-17 2018-02-28 カウンシル,インコーポレーテッド System and method for detecting genetic mutation
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
WO2014018460A1 (en) * 2012-07-24 2014-01-30 Sequenta, Inc. Single cell analysis using sequence tags
CN105264088B (en) 2012-08-08 2018-12-28 豪夫迈·罗氏有限公司 Improve the dynamic range of multiple epitopes in identification of cell
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
WO2014210353A2 (en) 2013-06-27 2014-12-31 10X Technologies, Inc. Compositions and methods for sample processing
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
JP6454281B2 (en) 2012-11-05 2019-01-16 タカラ バイオ ユーエスエー, インコーポレイテッド Nucleic acid that bar-coded
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
WO2014116729A2 (en) * 2013-01-22 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of hla loci with ultra-deep shotgun sequencing
EP2948252A1 (en) 2013-01-24 2015-12-02 SABIC Global Technologies B.V. Microwell plate made from a polyester-polycarbonate
US10190963B2 (en) 2013-02-01 2019-01-29 Becton, Dickinson And Company Methods and systems for assessing sample behavior in a flow cytometer
EP2954104A4 (en) 2013-02-08 2016-08-24 10X Genomics Inc Polynucleotide barcode generation
WO2014126937A1 (en) 2013-02-14 2014-08-21 The Board Of Trustees Of The Leland Stanford Junior University Suspension arrays and multiplexed assays based thereon
US9621600B2 (en) 2013-02-26 2017-04-11 PortAura Group Method and system for providing recommendations using location information
US20140274811A1 (en) 2013-03-14 2014-09-18 Lyle J. Arnold Methods for Amplifying a Complete Genome or Transcriptome
GB2525568A (en) 2013-03-15 2015-10-28 Abvitro Inc Single cell bar-coding for antibody discovery
WO2014165762A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
EP2805769A1 (en) 2013-05-24 2014-11-26 European Molecular Biology Laboratory Methods for nano-scale single cell analysis
JP2016525344A (en) 2013-06-12 2016-08-25 ザ ジェネラル ホスピタル コーポレイション The method for multiplex detection of target molecules, kits, and systems, as well as their use
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
US20150011398A1 (en) 2013-06-17 2015-01-08 Kim Lewis Methods for quantitative determination of protein-nucleic acid interactions in complex mixtures
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
KR20160055168A (en) 2013-08-28 2016-05-17 셀룰러 리서치, 인크. Massively parallel single cell analysis
US20150066385A1 (en) 2013-08-30 2015-03-05 10X Technologies, Inc. Sequencing methods
US20150132743A1 (en) 2013-09-04 2015-05-14 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
WO2015047186A1 (en) 2013-09-30 2015-04-02 Di Wu Methods to profile molecular complexes by using proximity bar-coding
CN105745528A (en) 2013-10-07 2016-07-06 赛卢拉研究公司 Methods and systems for digitally counting features on arrays
EP3089822A1 (en) 2013-12-30 2016-11-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
US20160340720A1 (en) 2014-02-13 2016-11-24 Cellular Research Methods and compositions for array-based counting
US20170292149A1 (en) 2014-03-05 2017-10-12 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
KR20170026383A (en) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. Analysis of nucleic acid sequences
CN106795553A (en) 2014-06-26 2017-05-31 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN107002076A (en) 2014-09-15 2017-08-01 阿布维特罗有限责任公司 High-throughput nucleotide library sequencing
CN107407691A (en) 2015-01-22 2017-11-28 贝克顿迪金森公司 Devices and systems for molecular barcoding of nucleic acid targets in single cells
US20180002738A1 (en) 2015-01-23 2018-01-04 Qiagen Sciences, Llc High multiplex pcr with molecular barcoding
EP3259371A1 (en) 2015-02-19 2017-12-27 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
EP3262189A1 (en) 2015-02-27 2018-01-03 Cellular Research, Inc. Methods and compositions for barcoding nucleic acids for sequencing
CN107208158A (en) 2015-02-27 2017-09-26 赛卢拉研究公司 Spatially addressable molecular barcoding
US20180073073A1 (en) 2015-03-18 2018-03-15 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
JP2018509915A (en) 2015-03-30 2018-04-12 セルラー リサーチ, インコーポレイテッド Methods and compositions for combinatorial barcoding
US20160312276A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US9618871B2 (en) 2015-04-28 2017-04-11 Kyocera Document Solutions Inc. Image forming apparatus
US20170073730A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for library normalization
CN108473984A (en) 2015-11-04 2018-08-31 阿特雷卡公司 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US20170314067A1 (en) 2016-05-02 2017-11-02 Cellular Research, Inc. Accurate molecular barcoding
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
TWI600309B (en) 2016-05-28 2017-09-21 Hon Hai Prec Ind Co Ltd Angle adjusting mechanism
US20170342405A1 (en) 2016-05-31 2017-11-30 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US20180037942A1 (en) 2016-08-03 2018-02-08 Cellular Research, Inc. Enzyme-independent molecular indexing
CN109791157A (en) 2016-09-26 2019-05-21 赛卢拉研究公司 Use the reagent measuring protein expression with bar coded oligonucleotide sequence

Also Published As

Publication number Publication date
US20180258500A1 (en) 2018-09-13
US20180002764A1 (en) 2018-01-04
WO2015031691A1 (en) 2015-03-05
US9637799B2 (en) 2017-05-02
CN105705659A (en) 2016-06-22
JP2019106998A (en) 2019-07-04
JP2016533187A (en) 2016-10-27
EP3039158A1 (en) 2016-07-06
US10151003B2 (en) 2018-12-11
US20190040474A1 (en) 2019-02-07
US20180291470A1 (en) 2018-10-11
KR20160055168A (en) 2016-05-17
GB2546833B (en) 2018-04-18
US9598736B2 (en) 2017-03-21
GB201609740D0 (en) 2016-07-20
US20160265069A1 (en) 2016-09-15
US20160010151A1 (en) 2016-01-14
US20180327867A1 (en) 2018-11-15
EP3039158B1 (en) 2018-11-14
US20160145683A1 (en) 2016-05-26
US9567645B2 (en) 2017-02-14
US10253375B1 (en) 2019-04-09
GB2525104B (en) 2016-09-28
US20180327866A1 (en) 2018-11-15
DK3039158T3 (en) 2019-03-04
US20150299784A1 (en) 2015-10-22
US9567646B2 (en) 2017-02-14
GB2525104A (en) 2015-10-14
SG11201601188TA (en) 2016-03-30
EP3480321A1 (en) 2019-05-08
GB201511591D0 (en) 2015-08-19
US20160258012A2 (en) 2016-09-08
GB2546833A (en) 2017-08-02
ES2711168T3 (en) 2019-04-30
US20190085412A1 (en) 2019-03-21
US10208356B1 (en) 2019-02-19
AU2014312208A1 (en) 2016-03-10
US10131958B1 (en) 2018-11-20

Similar Documents

Publication Publication Date Title
GB2513793B (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP2753914A4 (en) Automated sample handling instrumentation, systems, processes, and methods
SG178536A1 (en) Imaging and evaluating embryos, oocytes, and stem cells
GB201216076D0 (en) Methods for obtaining a sequence
IL225493D0 (en) Engineered nucleic acids and methods of use thereof
WO2014108810A8 (en) Sample preparation on a solid support
GB2474146A (en) Methods of determining the health status of an individual
WO2012024658A3 (en) Integrated analysis system
EA201591467A1 (en) Anti-lag-3 binding proteins
CO7151523A2 (en) Method and compositions for modification of target RNA-directed DNA and for modulation of transcription directed RNA
WO2011156529A3 (en) Methods and composition for multiplex sequencing
AU2019204045A1 (en) Methods of sample preparation
GB201308243D0 (en) High-throughput immune sequencing
MX2014002991A (en) Systems and methods for multi-analysis.
HK1221012A1 (en) Laboratory sample distribution system and laboratory automation system
GB2509668A (en) Bone fixation system with opposed mounting portions
WO2014145820A3 (en) Multiple tagging of long dna fragments
WO2012129363A3 (en) Single cell nucleic acid detection and analysis
MX2015001939A (en) Microcapsule compositions and methods.
MX2016007541A (en) Modified nucleic acid molecules and uses thereof.
WO2011094572A3 (en) Hanging drop devices, systems and/or methods
SG10201807532SA (en) Compositions and methods for detecting rare sequence variants
TW201448078A (en) Generalized virtual inspector
WO2014116851A3 (en) Methods and systems for using a cloud computing environment to share biological related data
MX2016010285A (en) Crispr enabled multiplexed genome engineering.