SG10201806890VA - Massively parallel single cell analysis - Google Patents

Massively parallel single cell analysis

Info

Publication number
SG10201806890VA
SG10201806890VA SG10201806890VA SG10201806890VA SG10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA SG 10201806890V A SG10201806890V A SG 10201806890VA
Authority
SG
Singapore
Prior art keywords
cells
single cell
massively parallel
parallel single
cell analysis
Prior art date
Application number
SG10201806890VA
Inventor
Christina Fan
Stephen Fodor
Glenn Fu
Geoffrey Facer
Julie Wilhelmy
Original Assignee
Cellular Res Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51519182&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10201806890V(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cellular Res Inc filed Critical Cellular Res Inc
Publication of SG10201806890VA publication Critical patent/SG10201806890VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50857—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/023—Sending and receiving of information, e.g. using bluetooth
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/101—Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

MASSIVELY PARALLEL SINGLE CELL ANALYSIS The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes). Fig. 1
SG10201806890VA 2013-08-28 2014-08-28 Massively parallel single cell analysis SG10201806890VA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361871232P 2013-08-28 2013-08-28
US201461952036P 2014-03-12 2014-03-12
US201462012237P 2014-06-13 2014-06-13

Publications (1)

Publication Number Publication Date
SG10201806890VA true SG10201806890VA (en) 2018-09-27

Family

ID=51519182

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201806890VA SG10201806890VA (en) 2013-08-28 2014-08-28 Massively parallel single cell analysis
SG11201601188TA SG11201601188TA (en) 2013-08-28 2014-08-28 Massively parallel single cell analysis

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201601188TA SG11201601188TA (en) 2013-08-28 2014-08-28 Massively parallel single cell analysis

Country Status (11)

Country Link
US (13) US9567645B2 (en)
EP (3) EP3039158B1 (en)
JP (2) JP6545682B2 (en)
KR (4) KR20230074639A (en)
CN (2) CN110964796B (en)
AU (3) AU2014312208B2 (en)
DK (1) DK3039158T3 (en)
ES (2) ES2857908T3 (en)
GB (2) GB2546833B (en)
SG (2) SG10201806890VA (en)
WO (1) WO2015031691A1 (en)

Families Citing this family (335)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7833709B2 (en) 2004-05-28 2010-11-16 Wafergen, Inc. Thermo-controllable chips for multiplex analyses
AU2010232439C1 (en) 2009-04-02 2017-07-13 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
ES2797448T3 (en) 2011-08-01 2020-12-02 Bio Rad Laboratories Cell capture system
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
ES2776673T3 (en) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Methods and uses for molecular tags
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN104736725A (en) 2012-08-13 2015-06-24 加利福尼亚大学董事会 Methods and systems for detecting biological components
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
EP2986741B1 (en) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Method for generating extended sequence reads
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
SG10201806890VA (en) 2013-08-28 2018-09-27 Cellular Res Inc Massively parallel single cell analysis
EP3055676A1 (en) 2013-10-07 2016-08-17 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
CN105849264B (en) 2013-11-13 2019-09-27 纽亘技术公司 For identifying the composition and method that repeat sequencing reading
BR112016011764B1 (en) * 2013-11-29 2021-06-22 Koninklijke Philips N.V. DEVICE FOR OPTICALLY CONTROLLING A CHEMICAL REACTION IN A REACTION CHAMBER COMPRISING A REAGENT FLUID, AND METHOD FOR OPTICALLY CONTROLLING A CHEMICAL REACTION IN A REACTION CHAMBER COMPRISING A REAGENT FLUID
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3957750A1 (en) 2013-12-20 2022-02-23 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
WO2015168161A2 (en) * 2014-04-29 2015-11-05 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
US10738352B2 (en) * 2014-05-02 2020-08-11 Idac Theranostics, Inc. Method for analyzing nucleic acid derived from single cell
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
CN107075581B (en) 2014-08-06 2022-03-18 纽亘技术公司 Digital measurement by targeted sequencing
WO2018176007A1 (en) * 2017-03-23 2018-09-27 BioSpyder Technologies, Inc. Modulation of targets in cellular pathways identified by resolution of stochastic gene expression
WO2016040476A1 (en) 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
IL299976A (en) 2014-10-17 2023-03-01 Illumina Cambridge Ltd Contiguity preserving transposition
LT3207134T (en) * 2014-10-17 2019-09-10 Illumina Cambridge Limited Contiguity preserving transposition
EP3209419A4 (en) 2014-10-22 2018-10-03 The Regents of The University of California High definition microdroplet printer
CN114807307A (en) 2014-10-29 2022-07-29 10X 基因组学有限公司 Methods and compositions for sequencing target nucleic acids
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
CN107407638B (en) 2014-12-03 2021-01-12 伊索普莱西斯公司 Analysis and screening of cellular secretion characteristics
US20160163235A1 (en) * 2014-12-09 2016-06-09 Avery Dennison Corporation Can End Label
US10616219B2 (en) 2014-12-11 2020-04-07 FlowJo, LLC Single cell data management and analysis systems and methods
ES2856843T3 (en) 2014-12-19 2021-09-28 Univ Ottawa Integration of nanopore sensors within microfluidic channel arrays using controlled rupture
CN112126675B (en) 2015-01-12 2022-09-09 10X基因组学有限公司 Method and system for preparing nucleic acid sequencing library and library prepared by using same
EP4112744A1 (en) 2015-02-04 2023-01-04 The Regents of the University of California Sequencing of nucleic acids via barcoding in discrete entities
GB201501907D0 (en) * 2015-02-05 2015-03-25 Technion Res & Dev Foundation System and method for single cell genetic analysis
CN107250379B (en) 2015-02-19 2021-12-28 贝克顿迪金森公司 High throughput single cell analysis combining proteomic and genomic information
WO2016134342A1 (en) 2015-02-20 2016-08-25 Wafergen, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
ES2906221T3 (en) * 2015-02-27 2022-04-13 Becton Dickinson Co Methods for barcoding nucleic acids for sequencing
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
CN107614700A (en) 2015-03-11 2018-01-19 布罗德研究所有限公司 Genotype and phenotype coupling
US20180284125A1 (en) * 2015-03-11 2018-10-04 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2016149418A1 (en) * 2015-03-18 2016-09-22 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
ES2934982T3 (en) * 2015-03-30 2023-02-28 Becton Dickinson Co Methods for encoding with combinatorial barcodes
ES2955916T3 (en) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Multiplex analysis of biological specimens of spatially distinguished nucleic acids
WO2016168584A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10788452B2 (en) * 2015-04-21 2020-09-29 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
US10677793B2 (en) * 2015-04-21 2020-06-09 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
CN107810277B (en) * 2015-04-21 2021-11-02 通用自动化实验技术公司 High resolution systems, kits, devices, and methods for high throughput microbiological applications
WO2016172373A1 (en) * 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
CN107849560B (en) * 2015-04-27 2021-10-22 阿布维特罗有限责任公司 Methods for sequencing, identifying, pairing and validating therapeutic agents and disease-specific antigens
DK3760737T3 (en) 2015-05-11 2023-04-11 Illumina Inc Platform for discovery and analysis of therapeutic agents
WO2016183289A1 (en) * 2015-05-12 2016-11-17 Wake Forest University Health Services Identification of genetic modifications
EP3303633B1 (en) * 2015-05-26 2024-02-21 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, and systems
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
CN108138225B (en) * 2015-07-27 2022-10-14 亿明达股份有限公司 Spatial localization of nucleic acid sequence information
WO2017031125A1 (en) * 2015-08-17 2017-02-23 The Regents Of The University Of California Microdroplet-based multiple displacement amplification (mda) methods and related compositions
JP6743150B2 (en) * 2015-08-28 2020-08-19 イルミナ インコーポレイテッド Single cell nucleic acid sequence analysis
US10619186B2 (en) 2015-09-11 2020-04-14 Cellular Research, Inc. Methods and compositions for library normalization
US11092607B2 (en) * 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
KR20180097536A (en) 2015-11-04 2018-08-31 아트레카, 인크. A combination set of nucleic acid barcodes for the analysis of nucleic acids associated with single cells
KR101745590B1 (en) * 2015-11-13 2017-06-09 고려대학교 산학협력단 Optical Microscopy for RNA Splicing on Single Molecules Using Scattering Intensity, Localized Surface Plasmon Resonance and Surface-Enhanced Raman Scattering
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
WO2017096158A1 (en) 2015-12-04 2017-06-08 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
WO2017096110A1 (en) * 2015-12-04 2017-06-08 Siemens Healthcare Diagnostics Inc. Method and device for optimizing the process of identification of pathogens
CA3006994A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
US20190144936A1 (en) * 2016-01-15 2019-05-16 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
US11254974B2 (en) * 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
EP3423599B1 (en) * 2016-03-04 2023-04-19 Abbott Diagnostics Scarborough, Inc. Device for automated nested recombinase polymerase amplification
JP6552720B2 (en) * 2016-03-28 2019-07-31 株式会社日立製作所 Single-cell biomolecule capture system
EP3443123B1 (en) * 2016-04-11 2021-08-11 Board of Regents, The University of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
US11702702B2 (en) 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
EP4269616A3 (en) * 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) * 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
JP6231709B1 (en) 2016-05-31 2017-11-15 シスメックス株式会社 Fluorescence image analyzer and analysis method
US10640824B1 (en) * 2016-06-03 2020-05-05 Verily Life Sciences Llc Methods of identifying nucleic acids from a single cell using an electrical cell-trapping array
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
EP3686287A1 (en) * 2016-06-28 2020-07-29 Hifibio Method for transcriptome analysis of single cells
WO2018017892A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
CN110088290A (en) 2016-08-10 2019-08-02 加利福尼亚大学董事会 Multiple displacement amplification and PCR are combined in lotion droplet
CN107058310A (en) * 2016-08-12 2017-08-18 艾吉泰康生物科技(北京)有限公司 A kind of amplicon library constructing method for improving gene low frequency abrupt climatic change sensitivity
US11174503B2 (en) 2016-09-21 2021-11-16 Predicine, Inc. Systems and methods for combined detection of genetic alterations
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
JP2019531730A (en) * 2016-09-28 2019-11-07 ジェネラル オートメーション ラボ テクノロジーズ インコーポレイテッド Bacterial community relationship determination and other high-throughput microbiology applied high resolution systems, kits, devices, and methods
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
JP7254349B2 (en) * 2016-10-26 2023-04-10 インテグレーテッド ナノ－テクノロジーズ，インコーポレイティド System and method for optical detection of biomolecular targets
JP7228510B2 (en) 2016-11-08 2023-02-24 ベクトン・ディキンソン・アンド・カンパニー Cell labeling method
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
JP7348066B2 (en) 2016-11-11 2023-09-20 アイソプレキシス コーポレイション Compositions and methods for simultaneous analysis of single cell genome, transcriptome and proteome
WO2018094109A1 (en) * 2016-11-17 2018-05-24 Denovo Sciences, Inc. System and method for retrieving and analyzing particles
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
US20180165414A1 (en) 2016-12-14 2018-06-14 FlowJo, LLC Applied Computer Technology for Management, Synthesis, Visualization, and Exploration of Parameters in Large Multi-Parameter Data Sets
CN110462053A (en) 2016-12-21 2019-11-15 加利福尼亚大学董事会 Unicellular gene order-checking is carried out using the drop based on hydrogel
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
PT3558510T (en) 2016-12-22 2023-01-30 Illumina Inc Array including sequencing primer and non-sequencing entity
KR101903255B1 (en) * 2016-12-29 2018-10-02 한국표준과학연구원 Sensor for detecting botulinum neurotoxin using spinning carbon-nanotube
US20200316589A1 (en) * 2017-01-04 2020-10-08 Carlos Genty A Multi-Well Device for the Processing, Testing, and Multiplexed Analysis of Intact, Fixed, Paraffin or Plastic Embedded (IFPE) Biological Materials
US10722880B2 (en) 2017-01-13 2020-07-28 Cellular Research, Inc. Hydrophilic coating of fluidic channels
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018144240A1 (en) * 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3585364B1 (en) 2017-02-24 2021-04-28 The Regents of the University of California Particle-drop structures and methods for making and using the same
CN110799654A (en) * 2017-03-21 2020-02-14 穆韦尔斯有限公司 Sealed micropore assay
JP2020513826A (en) * 2017-03-24 2020-05-21 ナショナル ユニヴァーシティー オブ シンガポール Multiplex detection method for molecules
EP4159871A1 (en) 2017-03-24 2023-04-05 Becton, Dickinson and Company Synthetic multiplets for multiplets determination
CN110678558B (en) 2017-05-02 2023-06-02 国立大学法人东京大学 Method for detecting non-destructive measurement information and genome-related information of single cells in integrity
CN110621782B (en) * 2017-05-02 2023-10-24 国立大学法人东京大学 Method for monitoring dynamic changes in cells or substances derived therefrom and cell classification method using same
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
CN111148846B (en) 2017-05-05 2024-06-18 西比欧生物科学公司 Method for capturing and barcoding discrete biological units in hydrogels
WO2018217933A1 (en) 2017-05-25 2018-11-29 FlowJo, LLC Visualization, comparative analysis, and automated difference detection for large multi-parameter data sets
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
JP2020522262A (en) 2017-06-05 2020-07-30 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Sample index addition for single cells
US11029242B2 (en) 2017-06-12 2021-06-08 Becton, Dickinson And Company Index sorting systems and methods
EP3655753A1 (en) 2017-07-18 2020-05-27 Becton, Dickinson and Company Dynamic interactive display of multi-parameter quantitative biological data
US10593082B2 (en) 2017-07-18 2020-03-17 Becton, Dickinson And Company Dynamic display of multi-parameter quantitative biological data
US10636182B2 (en) 2017-07-18 2020-04-28 Becton, Dickinson And Company Dynamic interactive display of multi-parameter quantitative biological data
CN107177693B (en) * 2017-07-19 2020-11-06 中山大学 Multiple PCR amplification method
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
CN111295245B (en) 2017-08-29 2021-03-16 伯乐实验室有限公司 Systems and methods for separating and analyzing cells
EP4379727A1 (en) 2017-09-25 2024-06-05 Becton, Dickinson and Company Immune receptor-barcode error correction
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
CN111148847B (en) * 2017-10-11 2024-05-07 深圳华大智造科技有限公司 Methods for improving nucleic acid loading and stability
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
EP4241882A3 (en) * 2017-10-27 2023-12-06 10X Genomics, Inc. Methods for sample preparation and analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019099908A1 (en) * 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019100024A1 (en) * 2017-11-20 2019-05-23 Freenome Holdings, Inc. Methods for reduction in required material for shotgun sequencing
WO2019104337A1 (en) * 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
AU2018378827B2 (en) * 2017-12-07 2023-04-13 Massachusetts Institute Of Technology Single cell analyses
EP3805405A1 (en) * 2017-12-14 2021-04-14 Ludwig-Maximilians-Universität München Single molecule detection or quantification by means of dna nanotechnology in micro-wells
CN111492068A (en) * 2017-12-19 2020-08-04 贝克顿迪金森公司 Particles associated with oligonucleotides
TWI804560B (en) * 2018-01-11 2023-06-11 美商奈諾卡福有限責任公司 Microfluidic cellular device and methods of use thereof
US20200340915A1 (en) * 2018-01-19 2020-10-29 Nitto Denko Corporation Flow path, measurement tape, and measurement device
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US20190276820A1 (en) * 2018-03-12 2019-09-12 Mantra Bio, Inc. Single extracellular vesicle multiplexed protein and rna analysis
JP7003763B2 (en) * 2018-03-19 2022-02-04 凸版印刷株式会社 Reagent cartridge
US11519027B2 (en) * 2018-03-30 2022-12-06 Massachusetts Institute Of Technology Single-cell RNA sequencing using click-chemistry
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11773441B2 (en) 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
US11254975B2 (en) * 2018-05-24 2022-02-22 National Center For Child Health And Development Method of amplifying a polynucleotide of interest
US10883912B2 (en) 2018-06-04 2021-01-05 Becton, Dickinson And Company Biexponential transformation for graphics display
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
WO2019241249A1 (en) * 2018-06-11 2019-12-19 The Brigham And Women's Hospital, Inc. Single cell cloning approaches for biological studies
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020010311A2 (en) * 2018-07-05 2020-01-09 Active Genomes Expressed Diagnostics, Inc Viral oncogene influences and gene expression patterns as indicators of early tumorigenesis
WO2020012057A1 (en) * 2018-07-13 2020-01-16 Universidad De Granada Preparation of nucleic acid libraries or gene banks
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830262A1 (en) 2018-08-03 2021-06-09 Becton Dickinson and Company Nuclei barcoding and capture in single cells
ES2945323T3 (en) 2018-08-17 2023-06-30 Becton Dickinson Co Barcoding by aptamers
WO2020046833A1 (en) 2018-08-28 2020-03-05 Cellular Research, Inc. Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
CN109112182A (en) * 2018-09-04 2019-01-01 安徽农业大学 The method of the single oocyte gene quantitative expression of one boar
WO2020061123A1 (en) * 2018-09-18 2020-03-26 California Institute Of Technology Systems and methods for dissecting heterogeneous cell populations
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
JP2022506546A (en) * 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
KR20210098491A (en) * 2018-11-30 2021-08-10 진인포섹, 아이엔씨. A method for generating random oligonucleotides and determining their sequence
EP3891303A4 (en) * 2018-12-07 2022-09-14 Qiagen Sciences LLC Methods for preparing cdna samples for rna sequencing, and cdna samples and uses thereof
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
TWI725686B (en) 2018-12-26 2021-04-21 財團法人工業技術研究院 Tubular structure for producing droplets and method for producing droplets
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
GB2580385B (en) * 2019-01-08 2022-10-12 Quantumdx Group Ltd Oligonucleotide deposition onto polypropylene substrates
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167830A1 (en) 2019-02-12 2020-08-20 Becton, Dickinson And Company Determining expressions of transcript variants and polyadenylation sites
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US20200263169A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (en) 2019-03-11 2021-12-07 10X基因组学有限公司 Systems and methods for processing optically labeled beads
US11609208B2 (en) * 2019-04-12 2023-03-21 Western Digital Technologies, Inc. Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles
US11738336B2 (en) 2019-04-12 2023-08-29 Western Digital Technologies, Inc. Spin torque oscillator (STO) sensors used in nucleic acid sequencing arrays and detection schemes for nucleic acid sequencing
US11579217B2 (en) 2019-04-12 2023-02-14 Western Digital Technologies, Inc. Devices and methods for frequency- and phase-based detection of magnetically-labeled molecules using spin torque oscillator (STO) sensors
US10633693B1 (en) * 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CA3138881A1 (en) 2019-05-07 2020-11-12 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
EP3973074A4 (en) 2019-05-22 2023-09-06 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
KR20220033484A (en) 2019-06-14 2022-03-16 바이오 래드 래버러토리스 인코오포레이티드 Systems and Methods for Automated Single Cell Processing and Analysis
CA3145243A1 (en) * 2019-06-27 2020-12-30 Cell Microsystems, Inc. Systems and methods for associating single cell imaging with rna transcriptomics
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US20220356462A1 (en) * 2019-07-11 2022-11-10 Tokyo University Of Science Foundation Method for amplifying nucleic acid using solid-phase carrier
WO2021011944A2 (en) * 2019-07-18 2021-01-21 Essenlix Corporation Imaging based homogeneous assay
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
EP4018001A4 (en) * 2019-08-19 2023-09-13 Universal Sequencing Technology Corporation Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing
US11208682B2 (en) 2019-09-13 2021-12-28 Western Digital Technologies, Inc. Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags
CN110951580B (en) * 2019-09-29 2022-05-20 中国科学院苏州生物医学工程技术研究所 High-throughput single-cell transcriptome and gene mutation integration analysis integrated device
GB2604481A (en) 2019-10-10 2022-09-07 1859 Inc Methods and systems for microfluidic screening
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
JP2023500679A (en) 2019-11-08 2023-01-10 ベクトン・ディキンソン・アンド・カンパニー Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
US11747329B2 (en) 2019-11-22 2023-09-05 Western Digital Technologies, Inc. Magnetic gradient concentrator/reluctance detector for molecule detection
WO2021113065A1 (en) 2019-12-04 2021-06-10 Becton, Dickinson And Company Magnetic capture bead mediated molecular barcoding of nucleic acid targets in single particles and compositions for use in the same
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CA3167719A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
US11512337B2 (en) 2020-01-13 2022-11-29 Fluent Biosciences Inc. Emulsion based drug screening
US20210214770A1 (en) 2020-01-13 2021-07-15 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
CN115698282A (en) 2020-01-13 2023-02-03 福路伦特生物科学公司 Single cell sequencing
EP4090762A1 (en) 2020-01-17 2022-11-23 Becton, Dickinson and Company Methods and compositions for single cell secretomics
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
CN114901831A (en) * 2020-01-23 2022-08-12 青岛华大智造普惠科技有限公司 Single cell sequencing based on droplet microfluidics and application
EP4097228A1 (en) 2020-01-29 2022-12-07 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
CN115427584A (en) 2020-01-31 2022-12-02 贝克顿迪金森公司 Mesophilic DNA polymerase extension blockers
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
WO2021163374A2 (en) 2020-02-12 2021-08-19 Becton, Dickinson And Company Intracellular abseq
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
ES2965354T3 (en) 2020-04-22 2024-04-12 10X Genomics Inc Methods for spatial analysis using targeted RNA deletion
WO2021222302A1 (en) * 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for increasing cell recovery efficiency
CN113567690A (en) * 2020-04-28 2021-10-29 深圳迎凯生物科技有限公司 Incubation assembly, incubation device and automatic analysis device
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
EP4158054A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021258024A1 (en) * 2020-06-19 2021-12-23 California Institute Of Technology Sensitive and multiplexed detection of nucleic acids and proteins for large scale serological testing
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11932901B2 (en) * 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116157534A (en) 2020-07-13 2023-05-23 贝克顿迪金森公司 cDNA labelling control for single cell analysis
EP4190910A4 (en) * 2020-07-29 2024-05-15 MGI Tech Co., Ltd. Method for loading nucleic acid molecule on solid support
US20220033810A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) * 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022067494A1 (en) * 2020-09-29 2022-04-07 Singleron (Nanjing) Biotechnologies, Ltd. Method for detection of whole transcriptome in single cells
WO2022081766A1 (en) * 2020-10-13 2022-04-21 Arizona Board Of Regents On Behalf Of Arizona State University Transmembrane sensors and molecular amplifiers for lysis-free detection of intracellular targets
WO2022082025A1 (en) * 2020-10-16 2022-04-21 Biomems Analytics Llc Cellular response analysis method
AU2021364598A1 (en) 2020-10-19 2023-06-08 Bio-Rad Laboratories, Inc. System and method for rapid multiplexed sample processing with applications for nucleic acid amplification assays
CN114480592A (en) * 2020-10-27 2022-05-13 重庆医科大学 SNPs detection technology based on UDG mediated PCR, magnetic nano enrichment and strand displacement reaction
EP3995178A1 (en) * 2020-11-09 2022-05-11 Université de Genève Method
CN116438316A (en) 2020-11-17 2023-07-14 贝克顿迪金森公司 Cell-free nucleic acid and single cell combinatorial analysis for oncology diagnostics
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US20220162695A1 (en) 2020-11-20 2022-05-26 Becton, Dickinson And Company Use of dextramer in single cell analysis
CN116635533A (en) 2020-11-20 2023-08-22 贝克顿迪金森公司 Profiling of high and low expressed proteins
CA3199189A1 (en) * 2020-11-25 2022-06-02 Gudrun Stengel Multiplexed profiling of rna and dna modifications
CN116569042A (en) 2020-12-09 2023-08-08 贝克顿迪金森公司 Multiplexed single cell immunoassays
US20220187286A1 (en) 2020-12-15 2022-06-16 Becton, Dickinson And Company Single cell secretome analysis
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022155282A1 (en) * 2021-01-14 2022-07-21 Integrated Dna Technologies, Inc. Methods for production and quantification of unique molecular identifier-labeled beads
US20220277808A1 (en) * 2021-02-19 2022-09-01 Twist Bioscience Corporation Libraries for identification of genomic variants
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022192671A1 (en) * 2021-03-12 2022-09-15 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
US11884977B2 (en) * 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
EP4301870A1 (en) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022226234A1 (en) * 2021-04-21 2022-10-27 Rutgers, The State University Of New Jersey Identifying microbial signatures and gene expression signatures
CN113358866B (en) * 2021-04-22 2023-06-23 四川大学华西医院 Homogeneous ultrasensitive two-dimensional visualization and fluorescence analysis method for tetanus antigen based on triple parallel hybridization chain reaction and application thereof
CN113150989A (en) * 2021-05-06 2021-07-23 上海交通大学医学院附属第九人民医院 Support for biomechanical bionic research
CN117642633A (en) * 2021-05-26 2024-03-01 德克萨斯大学系统董事会 Method and system for single cell protein analysis
EP4348245A1 (en) * 2021-05-28 2024-04-10 Fluent Biosciences Inc. Spatial transcriptomics in pips
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11427855B1 (en) 2021-06-17 2022-08-30 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CN113450336B (en) * 2021-07-01 2022-10-25 维柯基科技(上海)有限公司 Processing method and device of porous fluorescent microarray image, computer equipment and computer readable storage medium
WO2023034739A1 (en) 2021-08-30 2023-03-09 Becton, Dickinson And Company Template switch oligonucleotide (tso) for mrna 5' analysis
CN117897498A (en) 2021-08-31 2024-04-16 贝克顿迪金森公司 RNA preservation and recovery from immobilized cells
WO2023034790A1 (en) 2021-08-31 2023-03-09 Becton, Dickinson And Company Use of decoy polynucleotides in single cell multiomics
WO2023034872A1 (en) 2021-09-01 2023-03-09 Becton, Dickinson And Company Spatial multiomics using in situ reverse transcription
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023039433A1 (en) 2021-09-08 2023-03-16 Becton, Dickinson And Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides
CN117957330A (en) 2021-09-14 2024-04-30 贝克顿迪金森公司 Full length single cell RNA sequencing
CN114277091B (en) * 2021-09-17 2024-02-27 广东省人民医院 Method for constructing high-quality immune repertoire library
CN118234860A (en) * 2021-11-16 2024-06-21 生物辐射实验室股份有限公司 Methods and compositions for genotyping and phenotyping cells
WO2023122041A1 (en) * 2021-12-20 2023-06-29 Enumerix, Inc. Detection and digital quantitation of multiple targets
US11834714B2 (en) 2021-12-20 2023-12-05 Enumerix, Inc. Detection and digital quantitation of multiple targets
CN114214395A (en) * 2021-12-31 2022-03-22 上海星耀医学科技发展有限公司 Nucleic acid amplification method and kit for improving gene detection accuracy
CN114507743B (en) * 2022-01-28 2022-11-25 中国农业科学院植物保护研究所 RPA primer, probe and kit for rapidly detecting heterodera filipjevi and application of RPA primer, probe and kit
WO2023150764A1 (en) 2022-02-07 2023-08-10 Becton, Dickinson And Company Sorting of mrna and abseq containing barcoded beads by flow
WO2023150763A1 (en) 2022-02-07 2023-08-10 Becton, Dickinson And Company Preparing nucleic acid for further analysis of their sequence
WO2023154694A1 (en) 2022-02-08 2023-08-17 Becton, Dickinson And Company Reference beads for linking imaging and sequencing readouts with single-cell resolution
WO2023172977A1 (en) 2022-03-09 2023-09-14 Becton, Dickinson And Company Modified flow proxy assay prior to single-cell cite-seq
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
US20230383332A1 (en) * 2022-05-31 2023-11-30 Bio-Rad Laboratories, Inc. Methods and hydrogel compositions for partitioning biological samples
WO2023250283A1 (en) * 2022-06-23 2023-12-28 The Trustees Of The University Of Pennsylvania Patterning wettability in complex microfluidic channels for very large-scale generation of double emulsions
WO2024035789A1 (en) * 2022-08-10 2024-02-15 Becton, Dickinson And Company Highly efficient partition loading of single cells
WO2024073442A1 (en) * 2022-09-27 2024-04-04 Fred Hutchinson Cancer Center Methods for isolating, detecting, and quantifying intercellular proteins
WO2024097263A1 (en) 2022-11-01 2024-05-10 Becton, Dickinson And Company Bead-based single cell secretome analysis
WO2024097718A1 (en) 2022-11-02 2024-05-10 Becton, Dickinson And Company Polymerase mediated end modification of abseq
WO2024097719A1 (en) 2022-11-02 2024-05-10 Becton, Dickinson And Company Application for peptide nucleic acid (pna) blocker
US11802870B1 (en) 2022-12-13 2023-10-31 Panazee Kinetic immunoassay systems and methods

Family Cites Families (598)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4437975A (en) 1977-07-20 1984-03-20 Mobil Oil Corporation Manufacture of lube base stock oil
US4510244A (en) 1980-04-17 1985-04-09 The Board Of Trustees Of The Leland Stanford Jr. University Cell labeling with antigen-coupled microspheres
US4786600A (en) 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4725536A (en) 1985-09-19 1988-02-16 Genetics Institute, Inc. Reagent polynucleotide complex with multiple target binding regions, and kit and methods
US6150517A (en) 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Method and kits for the amplification and detection of nucleic acid sequences
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5656731A (en) 1987-10-15 1997-08-12 Chiron Corporation Nucleic acid-amplified immunoassay probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JP2650159B2 (en) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー Nucleic acid amplification method
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JPH04501353A (en) 1988-07-26 1992-03-12 ジエネラブス・テクノロジーズ・インコーポレイテツド RNA and DNA amplification methods
AU4829690A (en) 1988-12-16 1990-07-10 Siska Diagnostics, Inc. Self-sustained, sequence replication system
IE66597B1 (en) 1989-05-10 1996-01-24 Akzo Nv Method for the synthesis of ribonucleic acid (RNA)
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US5925525A (en) 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DE69128545D1 (en) 1990-08-24 1998-02-05 Univ Tennessee Res Corp GENETIC FINGERPRINT TECHNIQUE WITH DNA REPLACEMENT
JP3164814B2 (en) 1990-08-27 2001-05-14 科学技術振興事業団 Preparation of homogenized cDNA library
WO1992007095A1 (en) 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
EP1231282A3 (en) 1990-12-06 2005-05-18 Affymetrix, Inc. Methods and compositions for identification of polymers
JPH04337446A (en) 1991-05-15 1992-11-25 Hitachi Ltd Method and device for measuring fine grain and constant quantity method
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
DE69233087T2 (en) 1991-11-22 2003-12-24 Affymetrix Inc N D Ges D Staat Process for the production of polymer arrays
US5384261A (en) 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5550215A (en) 1991-11-22 1996-08-27 Holmes; Christopher P. Polymer reversal on solid surfaces
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5324633A (en) 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
WO1995000530A1 (en) 1993-06-25 1995-01-05 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US6087186A (en) 1993-07-16 2000-07-11 Irori Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
US5500356A (en) 1993-08-10 1996-03-19 Life Technologies, Inc. Method of nucleic acid sequence selection
US6309823B1 (en) 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
US5681697A (en) 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US6090555A (en) 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
DE69503126T2 (en) 1994-05-05 1998-11-12 Beckman Instruments Inc REPETITIVE OLIGONUCLEOTIDE MATRIX
US5571639A (en) 1994-05-24 1996-11-05 Affymax Technologies N.V. Computer-aided engineering system for design of sequence arrays and lithographic masks
US6495518B1 (en) 1994-06-13 2002-12-17 Vanderbilt University Method for importing biologically active molecules into cells
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6600996B2 (en) 1994-10-21 2003-07-29 Affymetrix, Inc. Computer-aided techniques for analyzing biological sequences
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
ATE340866T1 (en) 1994-10-28 2006-10-15 Gen Probe Inc COMPOSITIONS AND METHODS FOR THE SIMULTANEOUS DETECTION AND QUANTIFICATION OF A MAJORITY OF SPECIFIC NUCLIC ACID SEQUENCES
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
KR19990008052A (en) 1995-04-25 1999-01-25 마이클 피 노바 Remotely programmable matrix with storage device and uses thereof
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US5763175A (en) 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6147205A (en) 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5830712A (en) 1996-02-06 1998-11-03 Allelix Biopharmaceuticals Inc. Selective template deletion method
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
WO1997043611A1 (en) 1996-05-16 1997-11-20 Affymetrix, Inc. Systems and methods for detection of labeled materials
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
JPH1021373A (en) 1996-07-05 1998-01-23 Fuji Photo Film Co Ltd Picture analyzing device
US20020172953A1 (en) 1996-07-29 2002-11-21 Mirkin Chad A. Movement of biomolecule-coated nanoparticles in an electric field
US7169556B2 (en) 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6100029A (en) 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US5935793A (en) 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6117631A (en) 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
AU736321B2 (en) 1997-05-23 2001-07-26 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6033860A (en) 1997-10-31 2000-03-07 Affymetrix, Inc. Expression profiles in adult and fetal organs
WO1999028505A1 (en) 1997-12-03 1999-06-10 Curagen Corporation Methods and devices for measuring differential gene expression
US6235433B1 (en) 1997-12-19 2001-05-22 Nec Corporation High molecular gel electrolyte and secondary battery using the same
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US5936324A (en) 1998-03-30 1999-08-10 Genetic Microsystems Inc. Moving magnet scanner
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6653077B1 (en) 1998-09-04 2003-11-25 Lynx Therapeutics, Inc. Method of screening for genetic polymorphism
US6262216B1 (en) 1998-10-13 2001-07-17 Affymetrix, Inc. Functionalized silicon compounds and methods for their synthesis and use
US6480791B1 (en) 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US6197554B1 (en) 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US6436675B1 (en) * 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US20020019005A1 (en) 1999-02-18 2002-02-14 Arcaris, Inc. Process for identification of genes encoding proteins having cell proliferation-promoting activity
US6629040B1 (en) 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
WO2000058516A2 (en) 1999-03-26 2000-10-05 Whitehead Institute For Biomedical Research Universal arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
DE60045059D1 (en) 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Methods and probes for determining the concentration of nucleic acid molecules and methods for analyzing the data obtained
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
US6326148B1 (en) 1999-07-12 2001-12-04 The Regents Of The University Of California Detection of copy number changes in colon cancer
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
JP2001078768A (en) 1999-09-16 2001-03-27 Otsuka Pharmaceut Co Ltd Assay of gene expression
SE9903988D0 (en) 1999-11-03 1999-11-03 Amersham Pharm Biotech Ab Method of analysis
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
WO2001048242A2 (en) 1999-12-29 2001-07-05 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
US7022479B2 (en) 2000-01-24 2006-04-04 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
ATE322558T1 (en) 2000-01-24 2006-04-15 Compound Therapeutics Inc SENSITIVE AND MULTIPLEX DIAGNOSTIC TESTS FOR PROTEIN ANALYSIS
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
EP1990428B1 (en) 2000-02-07 2010-12-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US20050214825A1 (en) 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
DE10011579B4 (en) 2000-03-09 2007-06-06 BüHLER GMBH agitating mill
GB2364054B (en) 2000-03-24 2002-05-29 Smithkline Beecham Corp Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
NZ521593A (en) 2000-03-29 2004-11-26 Lgc Ltd Hybridisation beacon and method of rapid sequence detection and discrimination
DK2206791T3 (en) 2000-04-10 2016-10-24 Taxon Biosciences Inc Methods of study and genetic analysis of populations
US20030207300A1 (en) 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US7630063B2 (en) 2000-08-02 2009-12-08 Honeywell International Inc. Miniaturized cytometer for detecting multiple species in a sample
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
EP2100971A3 (en) 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
CA2418502A1 (en) 2000-08-11 2002-03-07 Nanosphere Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
DE60124363T2 (en) 2000-08-25 2007-09-06 Riken, Wako Method for the production of standardized and / or subtracted cDNA
JP4915492B2 (en) 2000-08-25 2012-04-11 独立行政法人理化学研究所 Method for creating a normalized / subtracted cDNA library
US6934408B2 (en) 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
JP4287652B2 (en) 2000-10-24 2009-07-01 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ Characterization of genomic DNA by direct multiple processing
WO2002046472A2 (en) 2000-12-08 2002-06-13 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
US20030049616A1 (en) 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
AU2002249116A1 (en) 2001-01-11 2002-09-19 Lion Bioscience Ag Gene library for screening methods
ATE546545T1 (en) 2001-01-25 2012-03-15 Luminex Molecular Diagnostics Inc POLYNUCLEOTIDES FOR USE AS TAGS AND TAG COMPLEMENTS, PREPARATION AND USE THEREOF
AU2002252279B2 (en) 2001-03-09 2005-05-12 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
JP2005233974A (en) 2001-03-21 2005-09-02 Olympus Corp Biochemical inspection method
US7027932B2 (en) 2001-03-21 2006-04-11 Olympus Optical Co., Ltd. Biochemical examination method
ATE431853T1 (en) 2001-03-28 2009-06-15 Nanosphere Inc BIO-BAR CODES BASED ON OLIGONUCLEOTIDE-MODIFIED PARTICLES
US20030170675A1 (en) 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
EP1377683A4 (en) 2001-04-11 2004-09-08 Us Gov Health & Human Serv Modified random primers for probe labeling
AUPR480901A0 (en) 2001-05-04 2001-05-31 Genomics Research Partners Pty Ltd Diagnostic method for assessing a condition of a performance animal
CA2344599C (en) 2001-05-07 2011-07-12 Bioneer Corporation Selective polymerase chain reaction of dna of which base sequence is completely unknown
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
CA2450139A1 (en) 2001-06-11 2002-12-19 Illumina, Inc Multiplexed detection methods
JP4244534B2 (en) 2001-06-12 2009-03-25 横河電機株式会社 Biochip
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US6830931B2 (en) 2001-07-12 2004-12-14 Automated Cell, Inc. Method and apparatus for monitoring of proteins and cells
WO2006071776A2 (en) 2004-12-23 2006-07-06 Ge Healthcare Bio-Sciences Corp. Ligation-based rna amplification
EP1478774A4 (en) 2001-10-09 2006-11-29 Nanosphere Inc Nanoparticles having oligonucleotides attached thereto and uses therefor
WO2003031591A2 (en) 2001-10-10 2003-04-17 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
AU2002359436A1 (en) 2001-11-13 2003-06-23 Rubicon Genomics Inc. Dna amplification and sequencing using dna molecules generated by random fragmentation
EP1448800A4 (en) 2001-11-21 2007-05-16 Applera Corp Digital assay
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
AU2003206095A1 (en) 2002-01-29 2003-09-02 Global Genomics Ab Methods and means for amplifying nucleic acid
EP1474772A4 (en) 2002-02-14 2005-11-09 Immunivest Corp Methods and algorithms for cell enumeration in a low-cost cytometer
US20030186251A1 (en) 2002-04-01 2003-10-02 Brookhaven Science Associates, Llc Genome sequence tags
US20050175993A1 (en) 2002-04-12 2005-08-11 Chia-Lin Wei Method for making full-length coding sequence cDNA libraries
US6808906B2 (en) 2002-05-08 2004-10-26 Rigel Pharmaceuticals, Inc. Directionally cloned random cDNA expression vector libraries, compositions and methods of use
US20070178478A1 (en) 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
GB0212391D0 (en) 2002-05-29 2002-07-10 Axis Shield Asa Assay
US20040096368A1 (en) 2002-06-28 2004-05-20 Igen International, Inc. Assay systems and components
US20050019776A1 (en) 2002-06-28 2005-01-27 Callow Matthew James Universal selective genome amplification and universal genotyping system
US7192700B2 (en) 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
EP1546721B2 (en) 2002-08-16 2017-05-17 Avantra Biosciences Corporation Substrates for isolating, reacting and microscopically analyzing materials
EP3067429A1 (en) 2002-09-03 2016-09-14 Quanta Biosciences, Inc. Improved methods for cdna synthesis
US7361821B2 (en) 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
WO2004031408A1 (en) 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US7822555B2 (en) 2002-11-11 2010-10-26 Affymetrix, Inc. Methods for identifying DNA copy number changes
US7424368B2 (en) 2002-11-11 2008-09-09 Affymetix, Inc. Methods for identifying DNA copy number changes
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
JP4395133B2 (en) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Single molecule amplification and detection of DNA
US20040214211A1 (en) 2003-01-23 2004-10-28 U.S. Genomics, Inc. Methods for analyzing polymer populations
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
JP4773338B2 (en) 2003-03-07 2011-09-14 ルビコン ゲノミクス， インコーポレイテッド Amplification and analysis of whole genome and whole transcriptome libraries generated by the DNA polymerization process
WO2006102264A1 (en) 2005-03-18 2006-09-28 Fluidigm Corporation Thermal reaction device and method for using the same
US7269518B2 (en) 2003-04-30 2007-09-11 Agilent Technologies, Inc. Chemical array reading
US20040259118A1 (en) 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
JP2006528482A (en) 2003-07-24 2006-12-21 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング Method for reverse transcription and / or amplification of nucleic acid
US20050106591A1 (en) 2003-08-13 2005-05-19 Affymetrix, Inc. Methods and kits for preparing nucleic acid samples
GB0319332D0 (en) 2003-08-16 2003-09-17 Astrazeneca Ab Amplification
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7341837B2 (en) 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
ATE461292T1 (en) 2003-09-10 2010-04-15 Althea Technologies Inc CREATION OF EXPRESSION PROFILES USING MICROARRAYS
EP1694869A2 (en) 2003-11-10 2006-08-30 Investigen, Inc. Methods of preparing nucleic acid for detection
CA2552858A1 (en) 2004-01-23 2005-08-04 Lingvitae As Improving polynucleotide ligation reactions
ATE451478T1 (en) 2004-02-12 2009-12-15 Population Genetics Technologi GENETIC ANALYSIS USING SEQUENCE-SPECIFIC SORTING
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US7432055B2 (en) 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
WO2005111243A2 (en) 2004-05-07 2005-11-24 Cepheid Multiplexed detection of biological agents
WO2005111242A2 (en) 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
US20060002824A1 (en) 2004-06-07 2006-01-05 Irm, Llc Dispensing systems, software, and related methods
US20060035258A1 (en) 2004-08-06 2006-02-16 Affymetrix, Inc. Methods for identifying DNA copy number changes
US20060041385A1 (en) 2004-08-18 2006-02-23 Bauer Kenneth D Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization
US7713697B2 (en) 2004-08-27 2010-05-11 Gen-Probe Incorporated Methods and kits for amplifying DNA
WO2006026828A1 (en) 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)
US20060073506A1 (en) 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
US7435084B2 (en) 2004-12-14 2008-10-14 Align Technology, Inc. System and methods for casting physical tooth model
PT1842226E (en) 2004-11-03 2010-11-29 Iris Molecular Diagnostics Inc Homogeneous analyte detection
JP5165383B2 (en) 2004-12-23 2013-03-21 アイ−スタツト・コーポレイシヨン Molecular diagnostic system and method
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
EP1845160A4 (en) 2005-02-10 2009-07-01 Riken Method for amplification of nucleotide sequence
WO2006099604A2 (en) 2005-03-16 2006-09-21 Compass Genetics, Llc Methods and compositions for assay readouts on multiple analytical platforms
US20090264635A1 (en) 2005-03-25 2009-10-22 Applera Corporation Methods and compositions for depleting abundant rna transcripts
US20060263789A1 (en) 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
US7695886B2 (en) 2005-05-19 2010-04-13 Fuji Xerox Co., Ltd. Process for producing resin particle liquid dispersion for electrostatic image developing toner, electrostatic image developing toner and production process thereof
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US20070065844A1 (en) 2005-06-08 2007-03-22 Massachusetts Institute Of Technology Solution-based methods for RNA expression profiling
US7796815B2 (en) 2005-06-10 2010-09-14 The Cleveland Clinic Foundation Image analysis of biological objects
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
EP2485052B1 (en) 2005-09-13 2015-05-06 Affymetrix, Inc. Encoded microparticles
CA2620081A1 (en) 2005-09-16 2007-03-29 454 Life Sciences Corporation Cdna library preparation
WO2007044974A2 (en) 2005-10-12 2007-04-19 The Research Foundation Of State University Of New York Absolute pcr quantification
US8313711B2 (en) 2005-11-01 2012-11-20 Freeslate, Inc. Liquid dispensing for high-throughput experimentation
US7375211B2 (en) 2005-11-18 2008-05-20 Kou Zhong C Method for detection and quantification of T-cell receptor Vβ repertoire
US20080070303A1 (en) * 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
US7636465B2 (en) 2005-12-09 2009-12-22 Cytyc Corporation Cross-frame object reconstruction for image-based cytology applications
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
US7537897B2 (en) * 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US20080038727A1 (en) 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
US7880142B2 (en) 2006-04-04 2011-02-01 Sidec Technologies Ab Extended electron tomography
US20080194414A1 (en) 2006-04-24 2008-08-14 Albert Thomas J Enrichment and sequence analysis of genomic regions
WO2007136874A2 (en) 2006-05-18 2007-11-29 President And Fellows Of Harvard College Genomic library construction
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
AU2007260676A1 (en) 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
EP2057465A4 (en) 2006-08-09 2010-04-21 Homestead Clinical Corp Organ-specific proteins and methods of their use
WO2008147428A1 (en) 2006-10-05 2008-12-04 Nanopoint, Inc. Systems and methods for active microfluidic cell handling
US8367051B2 (en) * 2006-10-09 2013-02-05 Carnegie Mellon University Preparation of functional gel particles with a dual crosslink network
SK50302009A3 (en) 2006-10-18 2010-03-08 Osaki Electric Co., Ltd. Electronic watthour meter
WO2008051928A2 (en) 2006-10-23 2008-05-02 The Salk Institute For Biological Studies Target-oriented whole genome amplification of nucliec acids
CA2958994C (en) 2006-11-15 2019-05-07 Biospherex Llc Kit for multiplex sequencing and ecogenomics analysis
US8050476B2 (en) 2006-11-22 2011-11-01 General Electric Company Heart rate demodulation of periodic movement in ultrasound data for object identification
EP2092322B1 (en) 2006-12-14 2016-02-17 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale fet arrays
WO2008087869A1 (en) 2007-01-16 2008-07-24 Olympus Corporation Fluorescent signal analyzing apparatus and fluorescent signal analyzing method
WO2008109207A2 (en) 2007-01-30 2008-09-12 The Regents Of The University Of California Methods and devices for biomolecular arrays
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
WO2008096318A2 (en) 2007-02-09 2008-08-14 Koninklijke Philips Electronics N.V. Identification system
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
US20110312511A1 (en) 2007-02-27 2011-12-22 Ola Winquist Multiplex Detection of Tumour Cells Using a Panel of Agents Binding to Extracellular Markers
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
KR100882711B1 (en) 2007-03-12 2009-02-06 성균관대학교산학협력단 Uracil-DNA glycosylase of Psychrobacter sp. HJ147 and use thereof
JP2008256428A (en) 2007-04-03 2008-10-23 Intec Systems Institute Inc Method for detecting block position of microarray image
US20080268508A1 (en) 2007-04-30 2008-10-30 Sowlay Mohankumar R Methods and kits for negative selection of desired nucleic acid sequences
US20080274458A1 (en) 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US20090061513A1 (en) 2007-05-15 2009-03-05 Picovitro Ab Cell sorting and cell cultivation methods
CN101720359A (en) 2007-06-01 2010-06-02 454生命科学公司 System and meth0d for identification of individual samples from a multiplex mixture
US8883691B2 (en) 2007-06-25 2014-11-11 Affymetrix, Inc. Encoded microparticles
WO2009031054A2 (en) 2007-06-29 2009-03-12 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
EP2036989B1 (en) 2007-09-12 2012-07-25 Institut Pasteur Polynucleotide suitable for single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution
WO2009048530A2 (en) 2007-10-05 2009-04-16 Panomics, Inc. Highly multiplexed particle-based assays
US10300482B2 (en) 2010-12-09 2019-05-28 Akonni Biosystems, Inc. Sample analysis system
EP2294408A4 (en) 2008-02-21 2011-05-25 Gentel Biosciences Inc Substrates for multiplexed assays and uses thereof
US8687189B2 (en) 2008-03-03 2014-04-01 Ajjer, Llc Analysis of arrays by laser induced breakdown spectroscopy
US8206925B2 (en) 2008-04-14 2012-06-26 Medical Diagnostic Laboratories, Llc Splice variants of human IL-23 receptor (IL-23R) mRNA and use of a delta 9 isoform in predicting inflammatory bowel diseases
US20090298709A1 (en) 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
DE102008025656B4 (en) 2008-05-28 2016-07-28 Genxpro Gmbh Method for the quantitative analysis of nucleic acids, markers therefor and their use
WO2009148560A2 (en) 2008-05-30 2009-12-10 Board Of Regents, The Universtiy Of Texas System Methods and compositions for nucleic acid sequencing
US20100069250A1 (en) 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US8835110B2 (en) 2008-11-04 2014-09-16 The Johns Hopkins University DNA integrity assay (DIA) for cancer diagnostics, using confocal fluorescence spectroscopy
EP2350657A4 (en) 2008-11-21 2013-06-12 Univ Saint Louis Biosensor for detecting multiple epitopes on a target
US20100159533A1 (en) 2008-11-24 2010-06-24 Helicos Biosciences Corporation Simplified sample preparation for rna analysis
US8492096B2 (en) 2008-12-24 2013-07-23 Boris Pasche TGFBR1 expression modifies risk for colorectal cancer
AU2010206843B2 (en) 2009-01-20 2015-01-29 The Board Of Trustees Of The Leland Stanford Junior University Single cell gene expression for diagnosis, prognosis and identification of drug targets
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
EP2404918B1 (en) 2009-03-05 2016-11-30 Daiichi Sankyo Company, Limited Pyridine derivative as ppary inhibitor
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
EP3002337B1 (en) 2009-03-30 2018-10-24 Illumina, Inc. Gene expression analysis in single cells
WO2010117626A2 (en) 2009-03-31 2010-10-14 Christophe Pierrat Lithography modelling and applications
AU2010232439C1 (en) 2009-04-02 2017-07-13 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
JPWO2010131645A1 (en) 2009-05-14 2012-11-01 和光純薬工業株式会社 Method for synthesizing and amplifying double-stranded DNA corresponding to RNA
FR2945545B1 (en) 2009-05-14 2011-08-05 Univ Aix Marseille Ii METHOD FOR DETECTION OF PROCARYOTE DNA EXTRACTED FROM A SAMPLE SAMPLE
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
US8330957B2 (en) 2009-06-29 2012-12-11 Hager Enviromental and Atmospheric Technologies, LLC Device and method for quantification of gases in plumes by remote sensing
EP2789689B1 (en) 2009-06-29 2016-04-27 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
EP3029141A1 (en) 2009-08-20 2016-06-08 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement
US8936762B2 (en) * 2009-09-01 2015-01-20 Trustees Of Boston University High throughput multichannel reader and uses thereof
CN107419002A (en) 2009-09-01 2017-12-01 皇家飞利浦电子股份有限公司 Apparatus and method for microarray selection
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US9488656B2 (en) 2009-09-30 2016-11-08 Quest Diagnostics Investments Incorporated BCR-ABL truncation mutations
KR102171849B1 (en) * 2009-12-07 2020-10-30 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Rna preparations comprising purified modified rna for reprogramming cells
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US10662474B2 (en) 2010-01-19 2020-05-26 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic DNA by whole genome sequencing
US20110245085A1 (en) 2010-01-19 2011-10-06 Rava Richard P Methods for determining copy number variations
CN102725424B (en) 2010-01-25 2014-07-09 Rd生物科技公司 Self-folding amplification of target nucleic acid
US8535889B2 (en) 2010-02-12 2013-09-17 Raindance Technologies, Inc. Digital analyte analysis
JP5901046B2 (en) 2010-02-19 2016-04-06 国立大学法人 千葉大学 Novel alternative splicing variant of OATP1B3 mRNA
WO2011106738A2 (en) 2010-02-25 2011-09-01 Fred Hutchinson Cancer Research Center Use of tcr clonotypes as biomarkers for disease
WO2011123246A2 (en) 2010-04-01 2011-10-06 Illumina, Inc. Solid-phase clonal amplification and related methods
PL2556171T3 (en) 2010-04-05 2016-04-29 Prognosys Biosciences Inc Spatially encoded biological assays
US20140227691A1 (en) 2010-05-14 2014-08-14 Fluidigm, Inc. Nucleic acid isolation methods
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP3425062B1 (en) 2010-06-09 2023-08-02 Keygene N.V. Combinatorial sequence barcodes for high throughput screening
US20120004132A1 (en) 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
JP5416279B2 (en) 2010-07-07 2014-02-12 株式会社アドバンテスト Test apparatus and test method
EP2407242A1 (en) * 2010-07-13 2012-01-18 Dublin City University Direct clone analysis and selection technology
US20120040843A1 (en) 2010-08-12 2012-02-16 Dublin City University Centrifugal capture system
WO2012027503A2 (en) 2010-08-24 2012-03-01 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
PT2623613T (en) 2010-09-21 2016-10-11 Population Genetics Tech Ltd Increasing confidence of allele calls with molecular counting
EP2436766A1 (en) 2010-09-29 2012-04-04 Deutsches Krebsforschungszentrum Means and methods for improved protein interaction screening
WO2012042374A2 (en) 2010-10-01 2012-04-05 Anssi Jussi Nikolai Taipale Method of determining number or concentration of molecules
US20120088691A1 (en) 2010-10-08 2012-04-12 Gao Chen Highly multiplexed real-time pcr using encoded microbeads
EP2625320B1 (en) * 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
EP2646795B1 (en) 2010-11-29 2019-02-20 Dako Denmark A/S Methods for analyzing images of specimens processed by a programmable quantitative assay
US9394511B2 (en) * 2010-12-05 2016-07-19 Wenbin Jiang Rapid single cell based parallel biological cell sorter
WO2012083225A2 (en) 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
JP6328934B2 (en) 2010-12-22 2018-05-23 ナテラ， インコーポレイテッド Noninvasive prenatal testing
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
DK3216878T3 (en) 2011-01-17 2019-06-11 Life Technologies Corp WORKING PROCEDURE FOR DETERMINING LIGANDS BY USING NUCLEAR ACIDS
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US10144950B2 (en) 2011-01-31 2018-12-04 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
JP6068365B2 (en) 2011-02-23 2017-01-25 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム Using template switches for DNA synthesis
WO2012129363A2 (en) * 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
KR20120110431A (en) 2011-03-29 2012-10-10 에스케이하이닉스 주식회사 A semiconductor memory apparatus
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN110016499B (en) 2011-04-15 2023-11-14 约翰·霍普金斯大学 Safety sequencing system
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP3415619B1 (en) 2011-04-28 2020-12-16 The Board of Trustees of the Leland Stanford Junior University Identification of polynucleotides associated with a sample
GB201108259D0 (en) 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
CN105999250B (en) 2011-05-24 2020-03-10 生物技术Rna制药有限公司 Individualized vaccines for cancer
US9518292B2 (en) 2011-05-26 2016-12-13 Brandeis University Methods for suppression PCR
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
WO2012177639A2 (en) 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
EP2724160A2 (en) 2011-06-27 2014-04-30 Life Technologies Corporation Acoustic cytometry methods and protocols
US9340826B2 (en) 2011-08-01 2016-05-17 Celemics, Inc. Method of preparing nucleic acid molecules
WO2013022961A1 (en) 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
JP5816486B2 (en) 2011-08-18 2015-11-18 オリンパス株式会社 Fluorescence observation apparatus, fluorescence observation system, and fluorescence image processing method of fluorescence observation apparatus
LT2756098T (en) 2011-09-16 2018-09-10 Lexogen Gmbh Method for making library of nucleic acid molecules
US9297047B2 (en) 2011-10-24 2016-03-29 Jennifer Furchak Molecular beacon based assay for the detection of biomarkers for breast cancer metastasis
ES2884100T3 (en) 2011-11-11 2021-12-10 Eli N Glezer Coagent-Assisted Binding Assay Procedures
KR101337094B1 (en) 2011-11-30 2013-12-05 삼성에스디에스 주식회사 Apparatus and method for sequence alignment
ES2626058T3 (en) 2011-12-22 2017-07-21 Ibis Biosciences, Inc. Primers and amplification methods
WO2013117595A2 (en) 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
WO2013120089A1 (en) 2012-02-10 2013-08-15 Raindance Technologies, Inc. Molecular diagnostic screening assay
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
EP2817418B1 (en) 2012-02-24 2017-10-11 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
ES2776673T3 (en) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Methods and uses for molecular tags
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
WO2013137737A1 (en) 2012-03-16 2013-09-19 Flexgen B.V. Methods of creating and screening of dna encoded combinatorial libraries of chemical antibodies
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
US9150905B2 (en) 2012-05-08 2015-10-06 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed PCR reactions
WO2013173394A2 (en) 2012-05-14 2013-11-21 Cb Biotechnologies, Inc. Method for increasing accuracy in quantitative detection of polynucleotides
EP2852682B1 (en) 2012-05-21 2017-10-04 Fluidigm Corporation Single-particle analysis of particle populations
WO2013176767A1 (en) * 2012-05-25 2013-11-28 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
CA2876209A1 (en) 2012-06-15 2013-12-19 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US9708654B2 (en) * 2012-06-15 2017-07-18 Board Of Regents, The University Of Texas System High throughput sequencing of multiple transcripts
CN104619894B (en) 2012-06-18 2017-06-06 纽亘技术公司 For the composition and method of the Solid phase of unexpected nucleotide sequence
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
JP6285929B2 (en) 2012-07-17 2018-02-28 カウンシル，インコーポレーテッド System and method for detecting genetic variation
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
SG11201500313YA (en) * 2012-07-24 2015-02-27 Sequenta Inc Single cell analysis using sequence tags
US10870099B2 (en) 2012-07-26 2020-12-22 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
WO2014026032A2 (en) 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9701998B2 (en) * 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014031997A1 (en) 2012-08-24 2014-02-27 Yale University System, device and method for high-throughput multi-plexed detection
CA2886974C (en) 2012-10-17 2021-06-29 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
CA2889862C (en) 2012-11-05 2021-02-16 Rubicon Genomics, Inc. Barcoding nucleic acids
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
WO2014116729A2 (en) * 2013-01-22 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of hla loci with ultra-deep shotgun sequencing
US9168532B2 (en) 2013-01-24 2015-10-27 Sabic Global Technologies B.V. Microwell plate
US10017761B2 (en) 2013-01-28 2018-07-10 Yale University Methods for preparing cDNA from low quantities of cells
ES2931314T3 (en) 2013-02-01 2022-12-28 Becton Dickinson Co Methods and systems to evaluate the behavior of a sample in a flow cytometer
EP2954102B1 (en) 2013-02-08 2018-12-19 Bio-Rad Laboratories, Inc. Affinity-based partition assay for detection of target molecules
WO2014126937A1 (en) 2013-02-14 2014-08-21 The Board Of Trustees Of The Leland Stanford Junior University Suspension arrays and multiplexed assays based thereon
US9621600B2 (en) 2013-02-26 2017-04-11 PortAura Group Method and system for providing recommendations using location information
US20140274811A1 (en) 2013-03-14 2014-09-18 Lyle J. Arnold Methods for Amplifying a Complete Genome or Transcriptome
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014145458A1 (en) 2013-03-15 2014-09-18 Arizona Board Of Regents On Behalf Of Arizona State University Nucleic acid-tagged compositions and methods for multiplexed protein-protein interaction profiling
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
EP3415626B1 (en) 2013-03-15 2020-01-22 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
GB2525568B (en) 2013-03-15 2020-10-14 Abvitro Llc Single cell barcoding for antibody discovery
WO2014165762A1 (en) 2013-04-05 2014-10-09 Raindance Technologies, Inc. Rare cell analysis after negative selection
SG11201508460TA (en) 2013-04-25 2015-11-27 Firefly Bioworks Inc Multiplexed analysis of target nucleic acids
US10822605B2 (en) 2013-05-03 2020-11-03 Gensinta, Inc. Method and apparatus for producing sequence verified DNA
EP2805769A1 (en) 2013-05-24 2014-11-26 European Molecular Biology Laboratory Methods for nano-scale single cell analysis
WO2014201273A1 (en) 2013-06-12 2014-12-18 The Broad Institute, Inc. High-throughput rna-seq
ES2887726T3 (en) 2013-06-12 2021-12-27 Massachusetts Gen Hospital Methods, kits, and systems for the multiplexed detection of target molecules and their uses
US20150011398A1 (en) 2013-06-17 2015-01-08 Kim Lewis Methods for quantitative determination of protein-nucleic acid interactions in complex mixtures
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
MX361481B (en) 2013-06-27 2018-12-06 10X Genomics Inc Compositions and methods for sample processing.
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US20160153973A1 (en) 2013-07-09 2016-06-02 Lucas David Smith Device and method of rapid linker mediated label-based immunoassays
SG10201806890VA (en) 2013-08-28 2018-09-27 Cellular Res Inc Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CA2922926A1 (en) 2013-09-04 2015-03-12 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
CN105745334B (en) 2013-09-30 2019-12-10 吴迪 Method for profiling molecular complexes by using proximity barcoding
EP3055676A1 (en) 2013-10-07 2016-08-17 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
EP3875601A1 (en) 2013-10-17 2021-09-08 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2015061719A1 (en) 2013-10-25 2015-04-30 Massachusetts Institute Of Technology Method and apparatus for tracking cell identity
WO2015061844A1 (en) 2013-10-30 2015-05-07 The University Of Sydney Assay to stratify cancer patients
US10288608B2 (en) 2013-11-08 2019-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
CA2935122C (en) 2013-12-30 2023-09-19 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
WO2015117163A2 (en) 2014-02-03 2015-08-06 Integrated Dna Technologies, Inc. Methods to capture and/or remove highly abundant rnas from a heterogeneous rna sample
US20160340720A1 (en) 2014-02-13 2016-11-24 Cellular Research Methods and compositions for array-based counting
JP6608381B2 (en) 2014-02-26 2019-11-20 ヴェンタナ メディカル システムズ， インク． Photoselective methods for biological sample analysis
AU2015227054A1 (en) 2014-03-05 2016-09-22 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015168161A2 (en) 2014-04-29 2015-11-05 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
ES2861353T3 (en) 2014-05-19 2021-10-06 Univ Rice William M Allele-specific amplification using a composition of non-allele-specific primer oligonucleotides and overlapping allele-specific blocking agents
CN114214314A (en) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 Digital PCR barcoding
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3169799B1 (en) 2014-07-15 2019-09-04 Qiagen Sciences, LLC Semi-random barcodes for nucleic acid analysis
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
MX2017003382A (en) 2014-09-15 2017-11-20 Abvitro Llc High-throughput nucleotide library sequencing.
US9529672B2 (en) 2014-09-25 2016-12-27 Everspin Technologies Inc. ECC word configuration for system-level ECC compatibility
IL299976A (en) 2014-10-17 2023-03-01 Illumina Cambridge Ltd Contiguity preserving transposition
EP3234602B1 (en) 2014-12-19 2021-08-25 F. Hoffmann-La Roche AG Methods for identifying multiple epitopes in selected sub-populations of cells
US20160289669A1 (en) 2015-01-22 2016-10-06 Becton, Dickinson And Company Devices and systems for molecular barcoding of nucleic acid targets in single cells
EP3247804B1 (en) 2015-01-23 2020-08-05 Qiagen Sciences, LLC High multiplex pcr with molecular barcoding
EP4112744A1 (en) 2015-02-04 2023-01-04 The Regents of the University of California Sequencing of nucleic acids via barcoding in discrete entities
EP3256606B1 (en) 2015-02-09 2019-05-22 10X Genomics, Inc. Systems and methods for determining structural variation
CN107250379B (en) 2015-02-19 2021-12-28 贝克顿迪金森公司 High throughput single cell analysis combining proteomic and genomic information
ES2906221T3 (en) 2015-02-27 2022-04-13 Becton Dickinson Co Methods for barcoding nucleic acids for sequencing
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
CN107614700A (en) 2015-03-11 2018-01-19 布罗德研究所有限公司 Genotype and phenotype coupling
US20160266094A1 (en) 2015-03-13 2016-09-15 University Of Iowa Research Foundation Cellular barcode
WO2016149418A1 (en) 2015-03-18 2016-09-22 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
US10301660B2 (en) 2015-03-30 2019-05-28 Takara Bio Usa, Inc. Methods and compositions for repair of DNA ends by multiple enzymatic activities
ES2934982T3 (en) 2015-03-30 2023-02-28 Becton Dickinson Co Methods for encoding with combinatorial barcodes
US20180057873A1 (en) 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US9618871B2 (en) 2015-04-28 2017-04-11 Kyocera Document Solutions Inc. Image forming apparatus
WO2016191272A1 (en) 2015-05-22 2016-12-01 Sigma-Aldrich Co. Llc Methods for next generation genome walking and related compositions and kits
WO2016190795A1 (en) 2015-05-28 2016-12-01 Kaarel Krjutskov Blocking oligonucleotides
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
WO2017032808A1 (en) 2015-08-24 2017-03-02 Qiagen Gmbh Method for generating a rna-sequencing library
JP6743150B2 (en) 2015-08-28 2020-08-19 イルミナ インコーポレイテッド Single cell nucleic acid sequence analysis
US10619186B2 (en) 2015-09-11 2020-04-14 Cellular Research, Inc. Methods and compositions for library normalization
CA2999888C (en) 2015-09-24 2024-04-09 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
KR20180097536A (en) 2015-11-04 2018-08-31 아트레카, 인크. A combination set of nucleic acid barcodes for the analysis of nucleic acids associated with single cells
WO2017087873A1 (en) 2015-11-18 2017-05-26 Wafergen, Inc. Systems and methods for pooling samples from multi-well devices
WO2017096239A1 (en) 2015-12-04 2017-06-08 St. Jude Children's Research Hospital, Inc. Cloning and expression system for t-cell receptors
WO2017117358A1 (en) 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Digital protein quantification
US20170205404A1 (en) 2016-01-19 2017-07-20 General Electric Company Multifunctional beads and methods of use for capturing rare cells
US20170260584A1 (en) 2016-02-11 2017-09-14 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
WO2017164936A1 (en) 2016-03-21 2017-09-28 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells
WO2017173328A1 (en) 2016-04-01 2017-10-05 Baylor College Of Medicine Methods of whole transcriptome amplification
EP4269616A3 (en) 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
WO2017196768A1 (en) 2016-05-09 2017-11-16 President And Fellows Of Harvard College Self-targeting guide rnas in crispr system
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
TWI600309B (en) 2016-05-28 2017-09-21 Hon Hai Prec Ind Co Ltd Angle adjusting mechanism
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3686287A1 (en) 2016-06-28 2020-07-29 Hifibio Method for transcriptome analysis of single cells
EP3488239A1 (en) 2016-07-22 2019-05-29 Verily Life Sciences LLC Quantitative massively parallel proteomics
WO2018020489A1 (en) 2016-07-24 2018-02-01 Yeda Research And Development Co. Ltd. Methods and kits for analyzing dna binding moieties attached to dna
US20180037942A1 (en) 2016-08-03 2018-02-08 Cellular Research, Inc. Enzyme-independent molecular indexing
CA3033506A1 (en) 2016-08-10 2018-02-15 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic dna fragments
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018061695A1 (en) 2016-09-29 2018-04-05 富士フイルム株式会社 Method for designing primers for multiplex pcr
WO2018061699A1 (en) 2016-09-29 2018-04-05 富士フイルム株式会社 Method for designing primers for multiplex pcr
EP3519612B1 (en) 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
CN114875125A (en) 2016-10-19 2022-08-09 10X基因组学有限公司 Methods and systems for barcoding nucleic acid molecules of individual cells or cell populations
CN114774520A (en) 2016-11-17 2022-07-22 领星生物科技（上海）有限公司 System and method for detecting tumor development
WO2018111872A1 (en) 2016-12-12 2018-06-21 Grail, Inc. Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018119452A2 (en) 2016-12-22 2018-06-28 Guardant Health, Inc. Methods and systems for analyzing nucleic acid molecules
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10550429B2 (en) * 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
CA3050127A1 (en) 2017-01-12 2018-07-19 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
US20180208975A1 (en) 2017-01-20 2018-07-26 Merck Sharp & Dohme Corp. Assay for simultaneous genomic and proteomic analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
DK3583214T3 (en) 2017-02-02 2023-12-18 New York Genome Center Inc METHODS AND COMPOSITIONS FOR IDENTIFYING OR QUANTIFYING TARGETS IN A BIOLOGICAL SAMPLE
US20200115753A1 (en) 2017-03-17 2020-04-16 Massachusetts Institute Of Technology Methods for identifying and modulating co-occurant cellular phenotypes
JP2020513826A (en) 2017-03-24 2020-05-21 ナショナル ユニヴァーシティー オブ シンガポール Multiplex detection method for molecules
EP4159871A1 (en) 2017-03-24 2023-04-05 Becton, Dickinson and Company Synthetic multiplets for multiplets determination
CN111051526A (en) 2017-05-23 2020-04-21 生捷科技控股公司 Method for performing analysis of spatial distribution of biomolecules
AU2018273401A1 (en) 2017-05-23 2019-12-19 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
MA49352A (en) 2017-05-26 2020-04-08 Abvitro Llc HIGH YIELD POLYNUCLEOTIDE LIBRARY SEQUENCING AND TRANSCRIPTOME ANALYSIS
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US20200181606A1 (en) 2017-05-29 2020-06-11 President And Fellows Of Harvard College A Method of Amplifying Single Cell Transcriptome
JP2020522262A (en) 2017-06-05 2020-07-30 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Sample index addition for single cells
US20200217850A1 (en) 2017-09-15 2020-07-09 Apton Biosystems, Inc. Heterogeneous single cell profiling using molecular barcoding
CN111727248A (en) 2017-09-25 2020-09-29 弗雷德哈钦森癌症研究中心 Efficient targeted in situ whole genome profiling
EP4379727A1 (en) 2017-09-25 2024-06-05 Becton, Dickinson and Company Immune receptor-barcode error correction
WO2019076768A1 (en) 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As Method and kit for dna library preparation
US11702649B2 (en) 2017-10-23 2023-07-18 The Broad Institute, Inc. Single cell cellular component enrichment from barcoded sequencing libraries
EP3710594B1 (en) 2017-11-16 2023-07-19 The Regents of The University of Colorado, A Body Corporate Methods to measure functional heterogeneity among single cells
AU2018378827B2 (en) 2017-12-07 2023-04-13 Massachusetts Institute Of Technology Single cell analyses
WO2019113499A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. High-throughput methods for identifying gene interactions and networks
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
CN114807306A (en) 2017-12-08 2022-07-29 10X基因组学有限公司 Methods and compositions for labeling cells
CN111699388A (en) 2017-12-12 2020-09-22 10X基因组学有限公司 Systems and methods for single cell processing
CN111712579A (en) 2017-12-22 2020-09-25 10X基因组学有限公司 Systems and methods for processing nucleic acid molecules from one or more cells
KR20200106179A (en) 2018-01-05 2020-09-11 빌리언투원, 인크. Quality control template to ensure the effectiveness of sequencing-based assays
CN112004920A (en) 2018-02-05 2020-11-27 斯坦福大学托管董事会 System and method for multiple measurements of single cells and aggregated cells
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
WO2019178164A1 (en) 2018-03-12 2019-09-19 Silicon Valley Scientific, Inc. Method and apparatus for processing tissue and other samples encoding cellular spatial position information
US20210123044A1 (en) 2018-04-18 2021-04-29 The Regents Of The University Of California Method to Connect Chromatin Accessibility and Transcriptome
EP3784797A1 (en) 2018-04-27 2021-03-03 X Gen US Co. Methods and compositions for preparing polynucleotides
US11773441B2 (en) 2018-05-03 2023-10-03 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CN110499251A (en) 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 The extracellular vesica analysis chip of the unicellular source property of high flux multiparameter and application
US20210222163A1 (en) 2018-07-11 2021-07-22 X Gen Us Co. Transposome enabled dna/rna-sequencing (ted rna-seq)
EP3830262A1 (en) 2018-08-03 2021-06-09 Becton Dickinson and Company Nuclei barcoding and capture in single cells
US20210302422A1 (en) 2018-08-08 2021-09-30 Vanderbilt University Systems and methods for simultaneous detection of antigens and antigen specific antibodies
ES2945323T3 (en) 2018-08-17 2023-06-30 Becton Dickinson Co Barcoding by aptamers
WO2020046833A1 (en) 2018-08-28 2020-03-05 Cellular Research, Inc. Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
JP2022506546A (en) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
CN113330121A (en) 2018-12-17 2021-08-31 纳特拉公司 Method for circulating cell analysis
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020159757A1 (en) 2019-01-28 2020-08-06 Becton, Dickinson And Company Oligonucleotide-comprising cellular component-binding reagents and methods of using the same
US20200263169A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020219721A1 (en) 2019-04-23 2020-10-29 The Broad Institute, Inc. Compositions and methods characterizing metastasis
SG10201904897UA (en) 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
WO2021011433A1 (en) 2019-07-12 2021-01-21 New York Genome Center, Inc Methods and compositions for scalable pooled rna screens with single cell chromatin accessibility profiling
US20210039582A1 (en) 2019-08-07 2021-02-11 ZF Passive Safety Systems US Inc. Airbag package with improved packaging
JP2023500679A (en) 2019-11-08 2023-01-10 ベクトン・ディキンソン・アンド・カンパニー Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
EP4087945B1 (en) 2020-01-10 2024-03-06 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US20210214770A1 (en) 2020-01-13 2021-07-15 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
EP4090762A1 (en) 2020-01-17 2022-11-23 Becton, Dickinson and Company Methods and compositions for single cell secretomics
EP4097228A1 (en) 2020-01-29 2022-12-07 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
CN115427584A (en) 2020-01-31 2022-12-02 贝克顿迪金森公司 Mesophilic DNA polymerase extension blockers
WO2021163374A2 (en) 2020-02-12 2021-08-19 Becton, Dickinson And Company Intracellular abseq
WO2021168015A1 (en) 2020-02-17 2021-08-26 Universal Sequencing Technology Corporation Methods of barcoding nucleic acid for detection and sequencing
AU2021224760A1 (en) 2020-02-21 2022-09-15 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
US20210263019A1 (en) 2020-02-25 2021-08-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021178199A1 (en) 2020-03-05 2021-09-10 BioLegend, Inc. Methods and compositions for detecting targets
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116157534A (en) 2020-07-13 2023-05-23 贝克顿迪金森公司 cDNA labelling control for single cell analysis
US20220033810A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
JP2023539822A (en) 2020-08-20 2023-09-20 ベクトン・ディキンソン・アンド・カンパニー Blood cell lysers for isolating bacteria from blood cultures
CN116438316A (en) 2020-11-17 2023-07-14 贝克顿迪金森公司 Cell-free nucleic acid and single cell combinatorial analysis for oncology diagnostics
CN116635533A (en) 2020-11-20 2023-08-22 贝克顿迪金森公司 Profiling of high and low expressed proteins
US20220162695A1 (en) 2020-11-20 2022-05-26 Becton, Dickinson And Company Use of dextramer in single cell analysis
CN116569042A (en) 2020-12-09 2023-08-08 贝克顿迪金森公司 Multiplexed single cell immunoassays
WO2022143221A1 (en) 2020-12-31 2022-07-07 中国科学院北京基因组研究所(国家生物信息中心) Method and kit for labeling nucleic acid molecules
KR20230142832A (en) 2021-01-08 2023-10-11 셀라노메, 인크. Devices and methods for analyzing biological samples
CN117396613A (en) 2021-06-01 2024-01-12 10X基因组学有限公司 Methods and compositions for analyte detection and probe resolution

Also Published As

Publication number Publication date
EP3842542A1 (en) 2021-06-30
DK3039158T3 (en) 2019-03-04
US20190085412A1 (en) 2019-03-21
GB2546833B (en) 2018-04-18
CN110964796A (en) 2020-04-07
KR102182834B1 (en) 2020-11-27
KR102536833B1 (en) 2023-05-26
US20180258500A1 (en) 2018-09-13
US10927419B2 (en) 2021-02-23
EP3039158A1 (en) 2016-07-06
US20160258012A2 (en) 2016-09-08
ES2857908T3 (en) 2021-09-29
KR20230074639A (en) 2023-05-30
JP6891199B2 (en) 2021-06-18
US20150299784A1 (en) 2015-10-22
US20160145683A1 (en) 2016-05-26
US10253375B1 (en) 2019-04-09
US9567646B2 (en) 2017-02-14
JP2016533187A (en) 2016-10-27
US11702706B2 (en) 2023-07-18
KR20200133281A (en) 2020-11-26
AU2014312208B2 (en) 2019-07-25
GB201511591D0 (en) 2015-08-19
US20180327866A1 (en) 2018-11-15
GB2546833A (en) 2017-08-02
SG11201601188TA (en) 2016-03-30
GB201609740D0 (en) 2016-07-20
WO2015031691A1 (en) 2015-03-05
US10131958B1 (en) 2018-11-20
EP3480321B1 (en) 2020-12-09
ES2711168T3 (en) 2019-04-30
US20180327867A1 (en) 2018-11-15
US9598736B2 (en) 2017-03-21
CN105705659B (en) 2019-11-29
US20160010151A1 (en) 2016-01-14
EP3480321A1 (en) 2019-05-08
EP3039158B1 (en) 2018-11-14
US20180291470A1 (en) 2018-10-11
US10151003B2 (en) 2018-12-11
AU2014312208A1 (en) 2016-03-10
GB2525104A (en) 2015-10-14
US10954570B2 (en) 2021-03-23
US20180002764A1 (en) 2018-01-04
AU2022291534A1 (en) 2023-02-02
US20230295742A1 (en) 2023-09-21
JP2019106998A (en) 2019-07-04
JP6545682B2 (en) 2019-07-17
CN110964796B (en) 2024-04-05
US9567645B2 (en) 2017-02-14
AU2019204928A1 (en) 2019-07-25
US20210198754A1 (en) 2021-07-01
US9637799B2 (en) 2017-05-02
CN105705659A (en) 2016-06-22
KR20220075240A (en) 2022-06-07
EP3480321B8 (en) 2021-03-10
GB2525104B (en) 2016-09-28
KR102402446B1 (en) 2022-05-30
US10208356B1 (en) 2019-02-19
US20190040474A1 (en) 2019-02-07
AU2019204928B2 (en) 2022-11-17
US20160265069A1 (en) 2016-09-15
KR20160055168A (en) 2016-05-17
US11618929B2 (en) 2023-04-04

Similar Documents

Publication Publication Date Title
SG10201806890VA (en) Massively parallel single cell analysis
MX2016016902A (en) Methods of analyzing nucleic acids from individual cells or cell populations.
MX2022012196A (en) Methods for culturing cells and kits and apparatus for same.
PH12020551028A1 (en) Methods, kits and apparatus for expanding a population of cells
MX2022012147A (en) Methods for culturing cells and kits and apparatus for same.
EP4026905C0 (en) Methods for barcoding nucleic acid molecules from individual cells or cell populations
HK1250757A1 (en) Nucleic acid sequence analysis from single cells
MX2016016904A (en) Analysis of nucleic acid sequences.
MX2022010686A (en) Methods, kits, agents and apparatuses for transduction.
EP4303314A3 (en) Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection
GB2516196A (en) Methods, compositions, kits, and systems for selective enrichment of target cells
HK1217971A1 (en) Methods, compositions, and kits for nucleic acid analysis
WO2014165267A3 (en) Systems and methods for prenatal genetic analysis
MX2022007933A (en) Polymethine compounds and their use as fluorescent labels.
SG10201804883SA (en) Systems and methods for detection of cells using engineered transduction particles
GB2513793A (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2016033251A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
AR080009A1 (en) GENOMIC OBJECTIVE ALTERATION USING ZINC FINGER NUCLEASES
WO2014116851A3 (en) Methods and systems for using a cloud computing environment to share biological related data
AR093446A1 (en) ANTIHEMAGLUTININE ANTIBODIES AND METHODS OF USE
SG10201903822XA (en) Method for the enrichment of circulating tumor dna
EP3353280A4 (en) Mass tag analysis for rare cells and cell free molecules
EP3910072A3 (en) Maternal plasma transcriptome analysis by massively parallel rna sequencing
RS54073B1 (en) Stromal stem cells
MX2015018035A (en) Methods for predicting responses to chemical or biologic substances.