WO2016033251A3 - Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation - Google Patents

Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation Download PDF

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Publication number
WO2016033251A3
WO2016033251A3 PCT/US2015/047053 US2015047053W WO2016033251A3 WO 2016033251 A3 WO2016033251 A3 WO 2016033251A3 US 2015047053 W US2015047053 W US 2015047053W WO 2016033251 A3 WO2016033251 A3 WO 2016033251A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
compositions
methods
high efficiency
acid sequence
Prior art date
Application number
PCT/US2015/047053
Other languages
French (fr)
Other versions
WO2016033251A2 (en
Inventor
Doug AMORESE
Nurith Kurn
Jonathan Scolnick
Alexandra Hui WANG
Original Assignee
Nugen Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies, Inc. filed Critical Nugen Technologies, Inc.
Priority to EP15836587.4A priority Critical patent/EP3186418A2/en
Publication of WO2016033251A2 publication Critical patent/WO2016033251A2/en
Publication of WO2016033251A3 publication Critical patent/WO2016033251A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). The methods, compositions and kits provided herein can be useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
PCT/US2015/047053 2014-08-26 2015-08-26 Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation WO2016033251A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP15836587.4A EP3186418A2 (en) 2014-08-26 2015-08-26 Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462042240P 2014-08-26 2014-08-26
US62/042,240 2014-08-26
US201562188337P 2015-07-02 2015-07-02
US62/188,337 2015-07-02

Publications (2)

Publication Number Publication Date
WO2016033251A2 WO2016033251A2 (en) 2016-03-03
WO2016033251A3 true WO2016033251A3 (en) 2016-04-21

Family

ID=55400819

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/047053 WO2016033251A2 (en) 2014-08-26 2015-08-26 Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation

Country Status (2)

Country Link
EP (1) EP3186418A2 (en)
WO (1) WO2016033251A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US12005454B2 (en) 2021-01-11 2024-06-11 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2533882B (en) 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124336A2 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Partitioning and processing of analytes and other species
CA2953374A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
MX367432B (en) 2015-01-12 2019-08-08 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same.
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
US10240196B2 (en) * 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US20180016632A1 (en) * 2016-07-12 2018-01-18 Kapa Biosystems, Inc. System and method for transposase-mediated amplicon sequencing
EP4310183A3 (en) 2017-01-30 2024-02-21 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN108949911B (en) * 2017-05-25 2022-10-14 北京大学 Method for identifying and quantifying low frequency somatic mutations
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN107586835B (en) * 2017-10-19 2020-11-03 东南大学 Single-chain-linker-based construction method and application of next-generation sequencing library
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11365408B2 (en) * 2018-02-07 2022-06-21 Nugen Technologies, Inc. Library preparation
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
KR20210141449A (en) * 2018-12-28 2021-11-23 내셔널 유니버시티 오브 싱가포르 Methods for Targeted Complementary DNA Enrichment
CN113355750A (en) * 2021-01-06 2021-09-07 南京诺唯赞生物科技股份有限公司 rRNA silent RNA library construction method and kit

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059740A1 (en) * 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
US20130231253A1 (en) * 2012-01-26 2013-09-05 Doug Amorese Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library regeneration

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059740A1 (en) * 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
US20130231253A1 (en) * 2012-01-26 2013-09-05 Doug Amorese Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library regeneration

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US12005454B2 (en) 2021-01-11 2024-06-11 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same

Also Published As

Publication number Publication date
EP3186418A2 (en) 2017-07-05
WO2016033251A2 (en) 2016-03-03

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