DE602004026033D1 - Zweiendige sequenzierung - Google Patents

Zweiendige sequenzierung

Info

Publication number
DE602004026033D1
DE602004026033D1 DE602004026033T DE602004026033T DE602004026033D1 DE 602004026033 D1 DE602004026033 D1 DE 602004026033D1 DE 602004026033 T DE602004026033 T DE 602004026033T DE 602004026033 T DE602004026033 T DE 602004026033T DE 602004026033 D1 DE602004026033 D1 DE 602004026033D1
Authority
DE
Germany
Prior art keywords
sequencing
dna
secondary sequencing
genomic
amplifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004026033T
Other languages
English (en)
Inventor
Yi-Ju Chen
John H Leamon
Kenton Lohman
Michael T Ronan
Maithreyan Srinivasan
Jonathan Rothberg
Michael Weiner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
454 Life Science Corp
Original Assignee
454 Life Science Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32854649&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602004026033(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 454 Life Science Corp filed Critical 454 Life Science Corp
Publication of DE602004026033D1 publication Critical patent/DE602004026033D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/12—Measuring magnetic properties of articles or specimens of solids or fluids
    • G01R33/1269—Measuring magnetic properties of articles or specimens of solids or fluids of molecules labeled with magnetic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE602004026033T 2003-01-29 2004-01-28 Zweiendige sequenzierung Expired - Lifetime DE602004026033D1 (de)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US44347103P 2003-01-29 2003-01-29
US46507103P 2003-04-23 2003-04-23
US47631303P 2003-06-06 2003-06-06
US47659203P 2003-06-06 2003-06-06
US47660203P 2003-06-06 2003-06-06
US47650403P 2003-06-06 2003-06-06
US49798503P 2003-08-25 2003-08-25
PCT/US2004/002485 WO2004070005A2 (en) 2003-01-29 2004-01-28 Double ended sequencing

Publications (1)

Publication Number Publication Date
DE602004026033D1 true DE602004026033D1 (de) 2010-04-29

Family

ID=32854649

Family Applications (4)

Application Number Title Priority Date Filing Date
DE602004036672T Expired - Lifetime DE602004036672C5 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf Basis von Kügelchenemulsion
DE602004024034T Expired - Lifetime DE602004024034D1 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion
DE602004022253T Expired - Lifetime DE602004022253D1 (de) 2003-01-29 2004-01-28 Verfahren zur amplifikation und sequenzierung von nukleinsäuren
DE602004026033T Expired - Lifetime DE602004026033D1 (de) 2003-01-29 2004-01-28 Zweiendige sequenzierung

Family Applications Before (3)

Application Number Title Priority Date Filing Date
DE602004036672T Expired - Lifetime DE602004036672C5 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf Basis von Kügelchenemulsion
DE602004024034T Expired - Lifetime DE602004024034D1 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion
DE602004022253T Expired - Lifetime DE602004022253D1 (de) 2003-01-29 2004-01-28 Verfahren zur amplifikation und sequenzierung von nukleinsäuren

Country Status (10)

Country Link
US (15) US7323305B2 (de)
EP (8) EP1594980B2 (de)
JP (9) JP2007525151A (de)
CN (2) CN102212614B (de)
AT (4) ATE448302T1 (de)
AU (4) AU2004209001B2 (de)
CA (6) CA2513889A1 (de)
DE (4) DE602004036672C5 (de)
ES (5) ES2342665T3 (de)
WO (4) WO2004069849A2 (de)

Families Citing this family (1139)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002000938A2 (en) * 2000-06-26 2002-01-03 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6858413B2 (en) 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
MXPA02012739A (es) * 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
WO2002102820A1 (en) * 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US20050009022A1 (en) * 2001-07-06 2005-01-13 Weiner Michael P. Method for isolation of independent, parallel chemical micro-reactions using a porous filter
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
US20030108664A1 (en) * 2001-10-05 2003-06-12 Kodas Toivo T. Methods and compositions for the formation of recessed electrical features on a substrate
JP4192097B2 (ja) * 2001-10-25 2008-12-03 バル−イラン ユニバーシティ 相互作用型透明個別細胞バイオチッププロセッサー
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
DK1487978T3 (da) 2002-03-15 2009-03-23 Nuevolution As Forbedret fremgangsmåde til syntese af templatemolekyler
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
WO2004013070A2 (en) * 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
EP1545762A2 (de) * 2002-09-27 2005-06-29 Carlsberg A/S Räumlich kodierte polymermatrix
DK3299463T3 (da) 2002-10-30 2020-12-07 Nuevolution As Enzymatisk kodning
WO2004056994A2 (en) * 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
EP1594694A4 (de) * 2002-12-30 2010-01-20 Univ California Verfahren und vorrichtung zum nachweis und zur analyse von krankheitserregern
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
ES2342665T3 (es) * 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
EP1597395A2 (de) 2003-02-21 2005-11-23 Nuevolution A/S Methode zur herstellung einer bibliothek der zweiten generation
EP2365095A1 (de) 2003-02-26 2011-09-14 Callida Genomics, Inc. Zufallsarray DNA Analyse mittels Hybridisierung
IL154677A0 (en) * 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
FR2856498B1 (fr) * 2003-06-19 2005-09-30 Goulven Jean Alain Vernois Distribution sous atmosphere controlee
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
US7888110B2 (en) * 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
JP5183063B2 (ja) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
DK1670939T3 (da) * 2003-09-18 2010-03-01 Nuevolution As Fremgangsmåde til opnåelse af strukturel information om et kodet molekyle og fremgangsmåde til udvælgelse af forbindelser
EP1670945A2 (de) * 2003-09-19 2006-06-21 Applera Corporation Zur ausführung einer nukleotidamplifikation mit temperaturzyklus geeignete mikroplatten
WO2005054431A2 (en) * 2003-12-01 2005-06-16 454 Corporation Method for isolation of independent, parallel chemical micro-reactions using a porous filter
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
EP3175914A1 (de) 2004-01-07 2017-06-07 Illumina Cambridge Limited Verbesserungen in oder im zusammenhang mit molekül-arrays
CA2553833C (en) * 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
EP1723255B1 (de) * 2004-02-17 2010-12-29 Nuevolution A/S Anreicherungsverfahren mit eliminierung durch fehlpaarungshybridisierung
CA2902980A1 (en) * 2004-03-08 2005-09-29 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US20060030037A1 (en) 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
WO2006003664A1 (en) * 2004-07-07 2006-01-12 Seng Enterprises Ltd. Method and device for identifying an image of a well in an image of a well-bearing component
EP1781404A2 (de) * 2004-08-25 2007-05-09 Seng Enterprises Limited Verfahren und vorrichtung zur isolierung von zellen
CN101073002B (zh) 2004-09-15 2012-08-08 英特基因有限公司 微流体装置
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060110764A1 (en) * 2004-10-25 2006-05-25 Tom Tang Large-scale parallelized DNA sequencing
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7485451B2 (en) * 2004-11-18 2009-02-03 Regents Of The University Of California Storage stable compositions of biological materials
BRPI0515777A (pt) * 2004-12-11 2008-08-05 Cytogenix Inc biossìntese isenta de células de ácido nucléico de alta qualidade e usos dos mesmos
US8038964B2 (en) 2005-01-25 2011-10-18 Seng Enterprises Ltd. Device for studying individual cells
EP2230315A1 (de) * 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
JP2006211984A (ja) * 2005-02-04 2006-08-17 Univ Nagoya エマルジョンを利用した核酸増幅方法、及び核酸増幅反応用キット
US8374887B1 (en) 2005-02-11 2013-02-12 Emily H. Alexander System and method for remotely supervising and verifying pharmacy functions
US8315816B2 (en) * 2005-02-16 2012-11-20 Genetic Technologies Limited Methods of genetic analysis involving the amplification of complementary duplicons
CA2599576C (en) * 2005-03-10 2015-09-15 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
WO2006099579A2 (en) * 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US7604940B1 (en) * 2005-03-16 2009-10-20 Applied Biosystems, Llc Compositions and methods for analyzing isolated polynucleotides
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
WO2006110855A2 (en) * 2005-04-12 2006-10-19 454 Life Sciences Corporation Methods for determining sequence variants using ultra-deep sequencing
US8497069B2 (en) * 2005-04-29 2013-07-30 Synthetic Genomics, Inc. Amplification and cloning of single DNA molecules using rolling circle amplification
US9365893B2 (en) * 2005-05-10 2016-06-14 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods of mapping polymorphisms and polymorphism microarrays
EP1910537A1 (de) 2005-06-06 2008-04-16 454 Life Sciences Corporation Sequenzierung mit gepaarten enden
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
DK2258870T3 (da) 2005-06-09 2013-03-18 Glaxosmithkline Llc Fremgangsmåde til at identificere forbindelser der binder til et biologisk target-molekyle
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
JP2008546405A (ja) * 2005-06-23 2008-12-25 キージーン ナムローゼ フェンノートシャップ ハイスループットシーケンシング技術を使用して複雑なゲノムをシーケンシングするための改善された戦略
DE602006018744D1 (de) 2005-06-23 2011-01-20 Keygene Nv Strategien mit hohem durchsatz zur identifizierung und zum nachweis von polymorphismen
US20070031865A1 (en) * 2005-07-07 2007-02-08 David Willoughby Novel Process for Construction of a DNA Library
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US10083273B2 (en) * 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
CA2619686A1 (en) * 2005-08-19 2007-02-22 Commomwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
EP2660482B1 (de) * 2005-08-22 2019-08-07 Life Technologies Corporation Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
WO2007035742A2 (en) * 2005-09-16 2007-03-29 454 Life Sciences Corporation Cdna library preparation
CA2623539C (en) 2005-09-29 2015-12-15 Keygene N.V. High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
EP2546360A1 (de) 2005-10-07 2013-01-16 Callida Genomics, Inc. Selbstangeordnete einzelne Molekülarrays und Verwendungen davon
US20070141555A1 (en) * 2005-10-11 2007-06-21 Mordechai Deutsch Current damper for the study of cells
PL1948816T3 (pl) 2005-10-24 2012-07-31 Univ Johns Hopkins Ulepszone sposoby beamingu
KR100828714B1 (ko) 2005-10-25 2008-05-09 주식회사 엘지화학 리보핵산을 이용한 멀티플렉스 증폭방법
EP2368868A1 (de) 2005-10-28 2011-09-28 Praecis Pharmaceuticals Inc. Verfahren zur Identifikation von Verbindungen von Interesse mithilfe kodierter Bibliotheken
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US8288120B2 (en) * 2005-11-03 2012-10-16 Seng Enterprises Ltd. Method for studying floating, living cells
US20090161100A1 (en) * 2005-11-08 2009-06-25 Minot Michael J Fiber Optic Interrogated Microslide, Microslide Kits and Uses Thereof
WO2007055568A1 (en) * 2005-11-14 2007-05-18 Keygene N.V. Method for high throughput screening of transposon tagging populations and massive parallel sequence identification of insertion sites
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
ES2901551T3 (es) 2005-12-01 2022-03-22 Nuevolution As Métodos de codificación enzimática para la síntesis eficiente de bibliotecas grandes
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
ES2394633T3 (es) * 2005-12-22 2013-02-04 Keygene N.V. Estrategias mejoradas para elaboración de perfiles de transcritos usando tecnologías de secuenciación de alto rendimiento
DK3404114T3 (da) 2005-12-22 2021-06-28 Keygene Nv Fremgangsmåde til detektering af AFLP-baseret polymorfisme med højt gennemløb
US20100137163A1 (en) * 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
JP4931257B2 (ja) * 2006-01-19 2012-05-16 ザ リサーチ ファウンデーション オブ ステイト ユニバーシティ オブ ニューヨーク コード化ビーズおよび生物学的分子の検出および同定のための方法およびデバイス
US20090053773A1 (en) * 2006-01-20 2009-02-26 Toppan Printing Co., Ltd. Reaction Container and Dna Amplification Reaction Method
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
EP1979079A4 (de) 2006-02-03 2012-11-28 Integenx Inc Mikrofluidische vorrichtungen
DE102006005287B4 (de) * 2006-02-06 2012-12-27 Siemens Ag Verfahren zum Nachweis von Zielnukleinsäuren
WO2007091064A1 (en) * 2006-02-08 2007-08-16 Solexa Limited End modification to prevent over-representation of fragments
EP1987159B2 (de) 2006-02-08 2020-08-12 Illumina Cambridge Limited Verfahren zur sequenzierung einer polynukleotidmatrize
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
JP5808515B2 (ja) 2006-02-16 2015-11-10 ４５４ ライフ サイエンシーズ コーポレイション 核酸配列データのプライマー伸長誤差を補正するためのシステムおよび方法
US8460878B2 (en) 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for detecting cells and cellular components in small defined volumes
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
JP5180845B2 (ja) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド Ｄｎａアレイ上でのハイスループットゲノム配列決定
SG10201405158QA (en) * 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
CN101460953B (zh) 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
DK3239304T3 (da) * 2006-04-04 2020-10-26 Keygene Nv Højgennemløbsdetektering af molekylære markører baseret på AFLP og sekventering med højt gennemløb
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US10522240B2 (en) 2006-05-03 2019-12-31 Population Bio, Inc. Evaluating genetic disorders
US7702468B2 (en) * 2006-05-03 2010-04-20 Population Diagnostics, Inc. Evaluating genetic disorders
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20080003142A1 (en) * 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
WO2007136736A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Methods for nucleic acid sorting and synthesis
WO2007140015A2 (en) * 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8614278B2 (en) 2006-06-06 2013-12-24 Dow Corning Corporation Silicone acrylate hybrid composition and method of making same
US8569416B2 (en) 2006-06-06 2013-10-29 Dow Corning Corporation Single phase silicone acrylate formulation
KR101432698B1 (ko) * 2006-06-06 2014-08-22 다우 코닝 코포레이션 실리콘 아크릴레이트 하이브리드 조성물
US20080124707A1 (en) * 2006-06-09 2008-05-29 Agency For Science, Technology And Research Nucleic acid concatenation
EP2366801B1 (de) 2006-06-14 2016-10-19 Verinata Health, Inc Verfahren zur Diagnose fötaler Missbildungen
EP2029780A4 (de) * 2006-06-16 2010-03-31 Pacific Biosciences California Gesteuerte initiierung einer primärextension
US8178300B2 (en) 2006-07-12 2012-05-15 Keygene N.V. Method for the identification of the clonal source of a restriction fragment
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008039998A2 (en) * 2006-09-28 2008-04-03 President And Fellows Of Harvard College Methods for sequencing dna
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008045575A2 (en) * 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
WO2008052138A2 (en) * 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US8338109B2 (en) 2006-11-02 2012-12-25 Mayo Foundation For Medical Education And Research Predicting cancer outcome
CN103497991A (zh) 2006-11-06 2014-01-08 科隆迪亚戈有限公司 使用结合元件用于分析的装置和方法
WO2008070375A2 (en) * 2006-11-09 2008-06-12 Complete Genomics, Inc. Selection of dna adaptor orientation
CA2669728C (en) * 2006-11-15 2017-04-11 Biospherex Llc Multitag sequencing and ecogenomics analysis
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US20080145898A1 (en) * 2006-12-14 2008-06-19 Applera Corporation Sequencing methods
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
JP5622392B2 (ja) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション 大規模ｆｅｔアレイを用いた分析物測定のための方法および装置
US7932034B2 (en) * 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20110039303A1 (en) 2007-02-05 2011-02-17 Stevan Bogdan Jovanovich Microfluidic and nanofluidic devices, systems, and applications
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US9152150B1 (en) 2007-02-22 2015-10-06 Applied Biosystems, Llc Compositions, systems, and methods for immiscible fluid discrete volume manipulation
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP2010521156A (ja) 2007-03-16 2010-06-24 ４５４ ライフ サイエンシーズ コーポレイション Ｈｉｖ薬物耐性バリアントの検出のためのシステムおよび方法
US20100151465A1 (en) 2008-03-27 2010-06-17 Jingyue Ju Selective Capture and Release of Analytes
US20080243865A1 (en) * 2007-03-28 2008-10-02 Oracle International Corporation Maintaining global state of distributed transaction managed by an external transaction manager for clustered database systems
US20080239867A1 (en) * 2007-03-28 2008-10-02 Gilbert Donna J Adjustable stir
JP2008245612A (ja) * 2007-03-30 2008-10-16 Hitachi Ltd 試料調製法および装置
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
US20090105959A1 (en) * 2007-06-01 2009-04-23 Braverman Michael S System and method for identification of individual samples from a multiplex mixture
CN101802218A (zh) * 2007-06-28 2010-08-11 454生命科学公司 在核酸测序中用于自适应试剂控制的系统和方法
US7635566B2 (en) * 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US8454906B2 (en) * 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
WO2009012984A1 (en) 2007-07-26 2009-01-29 Roche Diagnostics Gmbh Target preparation for parallel sequencing of complex genomes
JP5020734B2 (ja) * 2007-07-31 2012-09-05 株式会社日立ハイテクノロジーズ 核酸解析方法及び装置
WO2009032167A1 (en) * 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
EP2201021A4 (de) 2007-08-29 2012-01-25 Applied Biosystems Llc Alternative nukleinsäuresequenzierverfahren
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
EP2201374B1 (de) * 2007-08-30 2015-10-07 Trustees Of Tufts College Verfahren zur bestimmung der konzentration eines analyten in lösung
CN101795744A (zh) * 2007-09-11 2010-08-04 阿尔利克斯公司 用于分选物体的结合方法和仪器
US7998676B2 (en) * 2007-09-13 2011-08-16 Arryx, Inc. Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics
US20090109416A1 (en) * 2007-09-13 2009-04-30 Applied Precision, Inc. Dispersing immersion liquid for high resolution imaging and lithography
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US20100304378A1 (en) * 2007-09-17 2010-12-02 Universite De Strasbourg Method for Detecting or Quantifying a Truncating Mutation
US20090118129A1 (en) * 2007-09-28 2009-05-07 Pacific Biosciences Of California, Inc. Virtual reads for readlength enhancement
EP2212434A1 (de) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase-ligation-sequenzierung
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
EP2053132A1 (de) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Anreicherung und Sequenzanalyse von Genombereichen
WO2009055597A2 (en) * 2007-10-25 2009-04-30 Monsanto Technology Llc Methods for identifying genetic linkage
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
EP2237887A2 (de) * 2007-12-26 2010-10-13 Seng Enterprises Ltd. Vorrichtung zur untersuchung von lebenden zellen
US8189186B2 (en) * 2007-12-27 2012-05-29 Lawrence Livermore National Security, Llc. Signal enhancement using a switchable magnetic trap
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
EP3699291A1 (de) * 2008-01-17 2020-08-26 Sequenom, Inc. Einzelmolekülnukleinsäurensequenzanalyseverfahren und zusammensetzungen
KR20110030415A (ko) * 2008-01-22 2011-03-23 인터젠엑스 인크. 만능 샘플 제조 시스템 및 집적 분석 시스템에서의 용도
US7767400B2 (en) * 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
EP2250283A2 (de) * 2008-02-12 2010-11-17 Nugen Technologies, Inc. Verfahren und zusammensetzungen zur isothermen amplifikation von nukleinsäuren
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
AU2009223671B2 (en) * 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
ES2528971T3 (es) 2008-03-17 2015-02-13 Stichting Genetwister Ip Descubrimientos de genes vinculados a la expresión
US10745740B2 (en) 2008-03-19 2020-08-18 Qiagen Sciences, Llc Sample preparation
US7846666B2 (en) * 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
BRPI0909212A2 (pt) * 2008-03-28 2015-08-18 Pacific Biosciences California Composições e método para sequeciamento de ácido nucléico
CA2719211C (en) * 2008-04-04 2017-01-24 Allopartis Biotechnologies, Inc. Method of enhancing enzyme activity
JP5227062B2 (ja) 2008-04-08 2013-07-03 株式会社日立製作所 Ｄｎａ分析装置
TWI460602B (zh) * 2008-05-16 2014-11-11 Counsyl Inc 廣用的懷孕前篩檢裝置
WO2009143603A1 (en) 2008-05-28 2009-12-03 Genomedx Biosciences, Inc. Systems and methods for expression-based discrimination of distinct clinical disease states in prostate cancer
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
WO2010008480A2 (en) 2008-06-25 2010-01-21 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
WO2010009426A2 (en) * 2008-07-17 2010-01-21 Life Technologies Corporation Devices and methods for reagent delivery
US20100068711A1 (en) 2008-07-18 2010-03-18 Xenomics, Inc. Methods of PCR-Based Detection of "Ultra Short" Nucleic Acid Sequences
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US8795961B2 (en) 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
EP2318552B1 (de) 2008-09-05 2016-11-23 TOMA Biosciences, Inc. Verfahren zum stratifizieren und annotieren der behandlungsoptionen eines krebsmedikaments
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US20100184045A1 (en) * 2008-09-23 2010-07-22 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US8222047B2 (en) * 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20100075862A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US20100075439A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US20100261189A1 (en) 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2340314B8 (de) 2008-10-22 2015-02-18 Illumina, Inc. Erhaltung von informationen in verbindung mit genomischer dna-methylierung
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP5794572B2 (ja) 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 核酸を修飾するためのトランスポゾン末端組成物及び方法
JP2012506705A (ja) * 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
CA2742298C (en) 2008-11-03 2019-09-10 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
JP2010110262A (ja) * 2008-11-06 2010-05-20 Hitachi Maxell Ltd ウェルプレートを用いた核酸増幅法
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
JP5798926B2 (ja) 2008-11-07 2015-10-21 シーケンタ インコーポレイテッド 配列解析によって病態をモニターする方法
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
GB2477705B (en) * 2008-11-17 2014-04-23 Veracyte Inc Methods and compositions of molecular profiling for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
JP5777523B2 (ja) 2008-12-05 2015-09-09 キージーン・エン・フェー ファルネセン合成酵素
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
CN102341691A (zh) 2008-12-31 2012-02-01 尹特根埃克斯有限公司 具有微流体芯片的仪器
CN102272334B (zh) 2009-01-13 2014-08-20 关键基因股份有限公司 新基因组测序策略
US20100179074A1 (en) * 2009-01-15 2010-07-15 Honeywell International Inc. Methods and apparatus for fluid delivery and removal of micron scale structures
EP2387627B1 (de) 2009-01-15 2016-03-30 Adaptive Biotechnologies Corporation Adaptive immunitätsprofilierung und verfahren zur erzeugung monoklonaler antikörper
EP3020830A1 (de) * 2009-01-20 2016-05-18 The Board Of Trustees Of The Leland Stanford Junior University Einzelzellengenexpression zur diagnose, prognose und identifikation von wirkstofftargets
US20100331204A1 (en) 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
KR101793744B1 (ko) 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 유동 포커싱 미세유동 장치의 규모 확장
EP2408936A4 (de) * 2009-03-18 2013-01-30 Sequenom Inc Verwendung wärmestabiler endonukleasen zur herstellung von reportermolekülen
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
GB0904934D0 (en) 2009-03-23 2009-05-06 Geneseqe As Method and apparatus for detecting molecules
EP3415235A1 (de) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation von mikrofluidiktröpfchen
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
WO2010117461A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System and method for preparing and using bulk emulsion
US9090663B2 (en) * 2009-04-21 2015-07-28 The Trustees Of Columbia University In The City Of New York Systems and methods for the capture and separation of microparticles
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
EP2248914A1 (de) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Die Verwendung von IIB-Restriktionsendonukleasen in Sequenzierungsanwendungen der zweiten Generation
EP2427575B1 (de) 2009-05-07 2018-01-24 Veracyte, Inc. Verfahren zur diagnose von schilddrüsenleiden
US8003329B2 (en) * 2009-05-12 2011-08-23 Becton Dickinson & Company Molecular counting by color-coded micelles
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
EP3663750B1 (de) 2009-05-29 2021-11-03 Life Technologies Corporation Nukleinsäuregerüstpolymerpartikel und verfahren zur herstellung und verwendung
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
WO2010141326A1 (en) 2009-06-02 2010-12-09 Integenx Inc. Fluidic devices with diaphragm valves
RU2559541C2 (ru) 2009-06-05 2015-08-10 Интедженкс Инк. Универсальная система подготовки образцов и применение в интегрированной системе анализа
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
JP2012531907A (ja) * 2009-07-02 2012-12-13 ザイジェム コーポレイション リミテッド 単一の反応槽において組合せられた、核酸ブロッキング、抽出、及び検出
JP5099455B2 (ja) * 2009-07-16 2012-12-19 独立行政法人科学技術振興機構 エマルジョンを用いたｒｎａの選択的増幅法
US10036063B2 (en) 2009-07-24 2018-07-31 Illumina, Inc. Method for sequencing a polynucleotide template
SG177733A1 (en) * 2009-07-29 2012-02-28 Pyrobett Pte Ltd Method and apparatus for conducting an assay
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
CN102597256B (zh) 2009-08-25 2014-12-03 伊鲁米那股份有限公司 选择和扩增多核苷酸的方法
KR20120089661A (ko) 2009-09-02 2012-08-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 분출 및 다른 기술을 사용하여 생성되는 다중 에멀션
EP2940153B1 (de) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System zum Mischen von Flüssigkeiten durch Koaleszenz mehrerer Emulsionen
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
WO2011032040A1 (en) 2009-09-10 2011-03-17 Centrillion Technology Holding Corporation Methods of targeted sequencing
US20110071033A1 (en) 2009-09-23 2011-03-24 Celmatix, Inc. Methods and devices for assessing infertility and/or egg quality
EP2473638B1 (de) 2009-09-30 2017-08-09 Natera, Inc. Verfahren zur nichtinvasiven pränatalen ploidiezuordnung
US8609339B2 (en) 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP3842150A1 (de) 2009-10-27 2021-06-30 President and Fellows of Harvard College Tröpfchenbildungsverfahren
EP2494069B1 (de) * 2009-10-30 2013-10-02 Roche Diagniostics GmbH Verfahren zum nachweis von ausgeglichenen chromosomalen aberrationen in genomen
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
JP2013509883A (ja) 2009-11-06 2013-03-21 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 臓器移植患者における移植片拒絶反応の非侵襲的診断方法
KR101183199B1 (ko) * 2009-11-16 2012-09-14 (주)지노믹트리 지노타이핑 방법
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
WO2011066476A1 (en) * 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US8584703B2 (en) * 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
WO2011071382A1 (en) 2009-12-10 2011-06-16 Keygene N.V. Polymorfphic whole genome profiling
US8932812B2 (en) 2009-12-17 2015-01-13 Keygene N.V. Restriction enzyme based whole genome sequencing
EP2513332A1 (de) 2009-12-18 2012-10-24 Keygene N.V. Verbesserte bulked-mutant-analyse
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
ES2704701T3 (es) 2010-01-19 2019-03-19 Verinata Health Inc Nuevo protocolo de preparación de bibliotecas de secuenciación
US20120100548A1 (en) 2010-10-26 2012-04-26 Verinata Health, Inc. Method for determining copy number variations
EP3151052A1 (de) 2010-02-01 2017-04-05 Illumina, Inc. Fokussierungsverfahren und optische systeme sowie anordnungen damit
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2537031A4 (de) 2010-02-18 2013-07-17 Anthony P Shuber Zusammensetzungen und verfahren zur behandlung von krebs
EP2539450B1 (de) * 2010-02-25 2016-02-17 Advanced Liquid Logic, Inc. Verfahren zur herstellung von nukleinsäure-bibliotheken
EP2539471B1 (de) 2010-02-26 2014-08-06 Life Technologies Corporation Verfahren zur sequenzierung bei verwendung einer modifizierte polymerase
US9678068B2 (en) * 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
ES2544635T3 (es) 2010-03-01 2015-09-02 Quanterix Corporation Métodos para extender el rango dinámico en ensayos para la detección de moléculas o partículas
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
CN202281746U (zh) 2010-03-06 2012-06-20 伊鲁米那股份有限公司 检测来自样品光信号的测定设备及其光学组件和光学系统
US20110311963A1 (en) * 2010-03-16 2011-12-22 Life Technologies Corporation Method and Apparatus for Addressable Flow Cells in Single Molecule Sequencing
EP2550351A4 (de) 2010-03-25 2014-07-09 Quantalife Inc Detektionssystem für assays auf tröpfchenbasis
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011127933A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
WO2011133695A2 (en) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
CN102858965A (zh) 2010-04-30 2013-01-02 霍夫曼-拉罗奇有限公司 纯化和利用来自超嗜热菌的无机焦磷酸酶的系统和方法
US9930297B2 (en) 2010-04-30 2018-03-27 Becton, Dickinson And Company System and method for acquiring images of medication preparations
EP2388337B1 (de) 2010-04-30 2014-07-02 Nxp B.V. Messgerät und Herstellungsverfahren dafür
CA2798431C (en) 2010-05-06 2018-10-23 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
CA2798635A1 (en) * 2010-05-06 2011-11-10 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
EP2566984B1 (de) 2010-05-07 2019-04-03 The Board of Trustees of the Leland Stanford Junior University Messung und vergleich von immundiversitäten durch screening mit hohem durchsatz
US8530158B2 (en) 2010-05-10 2013-09-10 Life Technologies Corporation System and method for processing a biological sample
CN103038635B (zh) 2010-05-11 2016-12-28 威拉赛特公司 用于诊断病状的方法和组合物
CA2798758C (en) 2010-05-18 2019-05-07 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2580353B1 (de) * 2010-06-11 2015-07-29 Life Technologies Corporation Alternative nukleotidflüsse in verfahren zur sequenzierung durch synthese
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
JP5968879B2 (ja) * 2010-06-30 2016-08-10 深▲せん▼華大基因股▲ふん▼有限公司 Ｄｎａ分子タグ技術及びｄｎａ不完全断片化技術に基づいたｐｃｒシークエンシング法及びそれを用いたｈｌａ遺伝子タイピング法
US9164070B2 (en) 2010-06-30 2015-10-20 Life Technologies Corporation Column adc
US20120001646A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Methods and apparatus for testing isfet arrays
JP2013533482A (ja) 2010-06-30 2013-08-22 ライフ テクノロジーズ コーポレーション イオン感応性電荷蓄積回路および方法
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
JP5876044B2 (ja) 2010-07-03 2016-03-02 ライフ テクノロジーズ コーポレーション 低濃度ドープドレインを有する化学的感応性センサ
US9650629B2 (en) * 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
WO2012008831A1 (en) 2010-07-13 2012-01-19 Keygene N.V. Simplified de novo physical map generation from clone libraries
CN103153466B (zh) 2010-07-22 2016-04-13 基因细胞生物系统有限公司 复合液体池
CN103392182B (zh) 2010-08-02 2017-07-04 众有生物有限公司 用于发现遗传疾病中致病突变的系统和方法
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
EP2606154B1 (de) 2010-08-20 2019-09-25 Integenx Inc. Integriertes analysesystem
US20120070829A1 (en) 2010-09-10 2012-03-22 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
EP2617061B1 (de) 2010-09-15 2021-06-30 Life Technologies Corporation Verfahren und vorrichtung zur analytmessung
CN105911126B (zh) 2010-09-24 2018-12-18 生命科技公司 匹配的晶体管对电路
KR20130113447A (ko) 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
EP3447155A1 (de) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwichassays in tröpfchen
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
WO2012051327A2 (en) * 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
EP2633069B1 (de) 2010-10-26 2015-07-01 Illumina, Inc. Sequenzierungsverfahren
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US9387476B2 (en) 2010-10-27 2016-07-12 Illumina, Inc. Flow cells for biological or chemical analysis
EP2632593B1 (de) * 2010-10-27 2021-09-29 Illumina, Inc. Durchflusszellen zur biologischen oder chemischen analyse
EP3141614B1 (de) 2010-10-27 2018-11-28 Life Technologies Corporation Prädiktives modell zur verwendung für sequenzierung nach synthese
CA3140602C (en) 2010-11-01 2023-10-03 Bio-Rad Laboratories, Inc. System for forming emulsions
EP3360963B1 (de) 2010-11-12 2019-11-06 Gen9, Inc. Verfahren und vorrichtungen für nukleinsäuresynthese
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
EP2643484A4 (de) 2010-11-22 2014-04-16 Univ California Verfahren zur identifizierung eines rna-transkripts zellulären ursprungs
EP2646573A1 (de) 2010-12-01 2013-10-09 MorphoSys AG Gleichzeitiger nachweis von biomolekülen in einzelzellen
WO2012082464A2 (en) 2010-12-14 2012-06-21 Life Technologies Corporation Systems and methods for run-time sequencing run quality monitoring
CA2821906C (en) 2010-12-22 2020-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP2659408B1 (de) 2010-12-29 2019-03-27 Life Technologies Corporation Zeitverzerrtes hintergrundsignal für sequenzierung-mit-synthese-operationen
EP3564395A1 (de) 2010-12-30 2019-11-06 Foundation Medicine, Inc. Optimierung von multigenanalysen von tumorproben
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
EP3582224A1 (de) 2010-12-30 2019-12-18 Life Technologies Corporation Modelle zur analyse von daten aus sequenzierung-mit-synthese-operationen
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US20130005591A1 (en) * 2011-01-13 2013-01-03 Halgen Corporation Method for parallel amplification of nucleic acids
EP2663655B1 (de) 2011-01-14 2015-09-02 Keygene N.V. Auf zufallssequenzen mit gepaartem ende basierende genotypisierung
WO2012100194A1 (en) 2011-01-20 2012-07-26 Ibis Biosciences, Inc. Microfluidic transducer
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US20130316358A1 (en) 2011-01-31 2013-11-28 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
JP6069224B2 (ja) 2011-01-31 2017-02-01 アプライズ バイオ， インコーポレイテッド 細胞において複数のエピトープを同定する方法
WO2012108920A1 (en) 2011-02-09 2012-08-16 Natera, Inc Methods for non-invasive prenatal ploidy calling
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US9121047B2 (en) * 2011-04-07 2015-09-01 Life Technologies Corporation System and methods for making and processing emulsions
WO2012139125A2 (en) 2011-04-07 2012-10-11 Life Technologies Corporation System and methods for making and processing emulsions
WO2012138921A1 (en) 2011-04-08 2012-10-11 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
AU2012249531B2 (en) 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
WO2012158603A2 (en) 2011-05-13 2012-11-22 Bioo Scientific Corporation Pooled adapter strategy for reducing bias in small rna characterization
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
KR20140034242A (ko) 2011-05-23 2014-03-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 다중 에멀젼을 포함하는 에멀젼의 제어
WO2012166647A1 (en) * 2011-05-27 2012-12-06 Life Technologies Corporation Methods for manipulating biomolecules
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2718309B1 (de) 2011-06-10 2018-04-04 Keygene N.V. Transkriptionsfaktor der die terpenbiosynthese beeinflusst
EP3517611A3 (de) 2011-06-15 2019-09-04 Gen9, Inc. Verfahren für präparatives in-vitro-klonen
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
AU2012281242B2 (en) 2011-07-08 2016-12-22 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
WO2013009654A1 (en) 2011-07-08 2013-01-17 Life Technologies Corporation Method and apparatus for automated sample manipulation
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
US9670538B2 (en) 2011-08-05 2017-06-06 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
EP4324935A2 (de) 2011-08-18 2024-02-21 Life Technologies Corporation Verfahren, systeme und computerlesbare medien zur herstellung von basenzuordnungen in der nukleinsäuresequenzierung
ES2704126T3 (es) 2011-08-23 2019-03-14 Found Medicine Inc Moléculas de fusión KIF5B-RET y usos de las mismas
AU2012300401B2 (en) 2011-08-26 2018-02-08 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
AU2012304328B2 (en) 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
SI3623481T1 (sl) 2011-09-23 2022-01-31 Illumina, Inc. Sestavki za sekvenciranje nukleinske kisline
EP2758552A4 (de) * 2011-09-23 2015-09-09 Univ Columbia Isolierung und anreicherung von nukleinsäuren auf einem mikrochip
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
US11008611B2 (en) * 2011-09-30 2021-05-18 Unm Rainforest Innovations Double gate ion sensitive field effect transistor
US10221454B2 (en) 2011-10-10 2019-03-05 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
CN102329876B (zh) * 2011-10-14 2014-04-02 深圳华大基因科技有限公司 一种测定待检测样本中疾病相关核酸分子的核苷酸序列的方法
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
CA2853088C (en) 2011-10-21 2018-03-13 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
DE102011085473A1 (de) 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Verfahren zur Identifikation von Aptameren
DK2773779T3 (da) 2011-11-04 2020-11-23 Population Bio Inc Fremgangsmåder og sammensætninger til diagnosticering, prognose og forebyggelse af neurologiske tilstande
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
AU2012335955A1 (en) 2011-11-07 2014-07-03 QIAGEN Redwood City, Inc. Methods and systems for identification of causal genomic variants
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US20130143774A1 (en) * 2011-12-05 2013-06-06 The Regents Of The University Of California Methods and compositions for generating polynucleic acid fragments
DK2788499T3 (en) 2011-12-09 2016-03-21 Illumina Inc Enhanced root for polymer tags
EP3904536A1 (de) 2011-12-09 2021-11-03 Adaptive Biotechnologies Corporation Diagnose von lymphoid-malignität und nachweis minimaler resterkrankungen
KR101830778B1 (ko) 2011-12-09 2018-02-22 삼성전자주식회사 발열 입자를 포함하는 오일 층을 이용하는 핵산 증폭 장치 및 방법
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
AU2012352153B2 (en) 2011-12-13 2018-07-26 Veracyte, Inc. Cancer diagnostics using non-coding transcripts
EP2795333A4 (de) 2011-12-20 2016-02-17 Univ Michigan Pseudogene und verwendungen davon
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
ES2953308T3 (es) 2011-12-22 2023-11-10 Harvard College Composiciones y métodos para la detección de analitos
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
US9506113B2 (en) 2011-12-28 2016-11-29 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US9823246B2 (en) 2011-12-28 2017-11-21 The Board Of Trustees Of The Leland Stanford Junior University Fluorescence enhancing plasmonic nanoscopic gold films and assays based thereon
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
US9222115B2 (en) 2011-12-30 2015-12-29 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
CA2860594A1 (en) 2012-01-09 2013-07-18 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
CN104334739A (zh) 2012-01-13 2015-02-04 Data生物有限公司 通过新一代测序进行基因分型
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
KR102028225B1 (ko) 2012-01-25 2019-10-02 젠셀 바이오시스템즈 리미티드 생체분자 분리
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
JP6196241B2 (ja) 2012-02-02 2017-09-13 インベンラ， インコーポレイテッド 生物学的に活性なポリペプチドのハイスループットスクリーニング
US8597882B2 (en) 2012-02-03 2013-12-03 Pyrobett Pte. Ltd. Method and apparatus for conducting an assay
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
US10407724B2 (en) 2012-02-09 2019-09-10 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
US9506117B2 (en) 2012-02-21 2016-11-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
EP3372694A1 (de) 2012-03-05 2018-09-12 Adaptive Biotechnologies Corporation Festlegung von gepaarten immunrezeptorketten aus frequenzabgestimmten untereinheiten
NO2694769T3 (de) 2012-03-06 2018-03-03
IN2014DN08312A (de) 2012-03-06 2015-05-15 Univ Oslo Hf
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US9193996B2 (en) 2012-04-03 2015-11-24 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
CN108485940B (zh) * 2012-04-12 2022-01-28 维里纳塔健康公司 拷贝数变异的检测和分类
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP3095879B1 (de) 2012-04-19 2018-09-19 Life Technologies Corporation Nukleinsäureamplifikation
CN104471075B (zh) 2012-04-19 2018-06-22 生命技术公司 核酸扩增
US20150344973A1 (en) * 2012-04-23 2015-12-03 Pathogenica, Inc. Method and System for Detection of an Organism
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9346063B2 (en) * 2012-04-30 2016-05-24 Life Technologies Corporation Centrifuge and method for loading a device
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
CN107586832B (zh) 2012-05-08 2021-03-30 适应生物技术公司 用于测量和校准多重pcr反应中的扩增偏倚的组合物和方法
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
CN109082462B (zh) * 2012-05-21 2022-10-28 斯克利普斯研究所 样品制备方法
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CN102766574B (zh) * 2012-05-24 2013-12-25 中国科学院北京基因组研究所 用于dna测序仪的反应仓
JP6200948B2 (ja) * 2012-05-25 2017-09-20 ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル マイクロ流体デバイス
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
EP2870264A4 (de) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative beurteilung der wiederherstellung eines menschlichen t-zellen-repertoires nach der transplantation allogener blutbildender stammzellen
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
GB201212775D0 (en) 2012-07-18 2012-08-29 Dna Electronics Ltd Sensing apparatus and method
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
EP2875156A4 (de) 2012-07-19 2016-02-24 Ariosa Diagnostics Inc Sequentielle ligationsbasierte multiplex-detektion von genetischen varianten
CN104685064A (zh) * 2012-07-24 2015-06-03 纳特拉公司 高度复合pcr方法和组合物
CA2880764C (en) 2012-08-03 2022-08-30 Foundation Medicine, Inc. Human papilloma virus as predictor of cancer prognosis
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US20150005199A1 (en) * 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005200A1 (en) * 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20140378322A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
DK3435084T3 (da) 2012-08-16 2023-05-30 Mayo Found Medical Education & Res Prostatakræftprognose under anvendelse af biomarkører
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2895620B1 (de) 2012-09-11 2017-08-02 Life Technologies Corporation Nukleinsäureamplifikation
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
CN104797718B (zh) * 2012-09-12 2020-05-08 加利福尼亚大学董事会 通过单链扩增和测序对单个细胞进行精确基因组测序
DK2895621T3 (da) 2012-09-14 2020-11-30 Population Bio Inc Fremgangsmåder og sammensætning til diagnosticering, prognose og behandling af neurologiske tilstande
US10233495B2 (en) 2012-09-27 2019-03-19 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
ES2660027T3 (es) 2012-10-01 2018-03-20 Adaptive Biotechnologies Corporation Evaluación de la inmunocompetencia por la diversidad de los receptores de inmunidad adaptativa y caracterización de la clonalidad
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US9177098B2 (en) 2012-10-17 2015-11-03 Celmatix Inc. Systems and methods for determining the probability of a pregnancy at a selected point in time
US10162800B2 (en) 2012-10-17 2018-12-25 Celmatix Inc. Systems and methods for determining the probability of a pregnancy at a selected point in time
EP2912196B1 (de) 2012-10-26 2018-08-08 Sysmex Corporation Emulsionssystem fur amplifizierung von nukleinsäuren auf emulsionsbasis
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
ES2886507T3 (es) 2012-10-29 2021-12-20 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
EP2914621B1 (de) 2012-11-05 2023-06-07 Foundation Medicine, Inc. Neuartige ntrk1-fusionsmoleküle und verwendungen davon
CA2888042A1 (en) 2012-11-27 2014-06-05 Gencell Biosystems Ltd. Handling liquid samples
EP2925893A4 (de) * 2012-12-03 2016-09-07 Elim Biopharmaceuticals Inc Zusammensetzungen und verfahren zur nukleinsäureherstellung und -analyse
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (de) 2012-12-14 2019-11-13 10X Genomics, Inc. Verfahren und systeme zur verarbeitung von polynukleotiden
US9836577B2 (en) 2012-12-14 2017-12-05 Celmatix, Inc. Methods and devices for assessing risk of female infertility
EP2746405B1 (de) 2012-12-23 2015-11-04 HS Diagnomics GmbH Verfahren und Primersätze zur PCR-Hochdurchsatzsequenzierung
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
EP2946345B1 (de) 2013-01-17 2024-04-03 Personalis, Inc. Verfahren und system zur phylogenetischen analyse
CA2898326C (en) 2013-01-18 2022-05-17 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
WO2014116729A2 (en) 2013-01-22 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of hla loci with ultra-deep shotgun sequencing
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
AU2014212152B2 (en) 2013-02-01 2020-02-06 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP2964781B1 (de) 2013-03-08 2018-01-10 Roche Diagnostics GmbH Bluttest auf egfr-mutationen
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
EP2971169A4 (de) 2013-03-13 2016-10-26 Abbott Molecular Inc Systeme und verfahren zur isolierung von nukleinsäuren
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
US10443092B2 (en) 2013-03-13 2019-10-15 President And Fellows Of Harvard College Methods of elongating DNA
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
JP2016512437A (ja) 2013-03-14 2016-04-28 アボツト・モレキユラー・インコーポレイテツド 多重メチル化特異的増幅システムおよび方法
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
US8778609B1 (en) 2013-03-14 2014-07-15 Good Start Genetics, Inc. Methods for analyzing nucleic acids
EP2971154A4 (de) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nukleinsäurekontrollpanels
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
EP2971152B1 (de) 2013-03-15 2018-08-01 The Board Of Trustees Of The Leland Stanford Junior University Identifikation und verwendung von zirkulierenden nukleinsäure-tumormarkern
CN105358709B (zh) 2013-03-15 2018-12-07 雅培分子公司 用于检测基因组拷贝数变化的系统和方法
EP2971130A4 (de) * 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
ES2877088T3 (es) 2013-03-15 2021-11-16 Guardant Health Inc Procedimiento para detectar cáncer
EP3415626B1 (de) 2013-03-15 2020-01-22 Lineage Biosciences, Inc. Verfahren und zusammensetzungen zum markieren und analysieren von proben
EP2971071B1 (de) 2013-03-15 2018-02-28 Illumina, Inc. Mit enzymen verknüpfte nukleotide
CN105051525B (zh) 2013-03-15 2019-07-26 生命科技公司 具有薄导电元件的化学设备
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
BR112015022490A2 (pt) 2013-03-15 2017-07-18 Veracyte Inc métodos e composições para classificação de amostras
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
WO2014149778A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensors with consistent sensor surface areas
WO2014149780A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
EP2971140B1 (de) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Verfahren zur beurteilung von kontamination in der dna-sequenzierung
JP6441893B2 (ja) * 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
US11859171B2 (en) 2013-04-17 2024-01-02 Agency For Science, Technology And Research Method for generating extended sequence reads
EP2986704B1 (de) * 2013-04-19 2019-04-03 Siemens Healthcare Diagnostics Inc. Kontaktloser mikrotropfenspender
RU2578009C2 (ru) * 2013-05-08 2016-03-20 Закрытое акционерное общество "ЕВРОГЕН" Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10160995B2 (en) 2013-05-13 2018-12-25 Qiagen Waltham, Inc. Analyte enrichment methods and compositions
CA2910019A1 (en) 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
KR101648252B1 (ko) * 2014-01-28 2016-08-16 연세대학교 산학협력단 염기서열 확인 과정에서 분리된 핵산 단편들을 회수하는 방법
US10526640B2 (en) 2013-06-19 2020-01-07 Industry-Academic Cooperation Foundation, Yonsei University Methods for retrieving sequence-verified nucleic acid fragments and apparatuses for amplifying sequence verified nucleic acid fragments
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
ES2700529T3 (es) 2013-07-01 2019-02-18 Illumina Inc Funcionalización de superficie sin catalizador e injerto de polímero
ES2719579T3 (es) 2013-07-03 2019-07-11 Illumina Inc Sistema para secuenciación por síntesis ortogonal
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
US9409139B2 (en) 2013-08-05 2016-08-09 Twist Bioscience Corporation De novo synthesized gene libraries
US9410977B2 (en) 2013-08-08 2016-08-09 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
US20150051117A1 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Assembly of Nucleic Acid Sequences in Emulsions
EP2840148B1 (de) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Verfahren für nukleinsäureamplifikation
EP2848698A1 (de) 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System und Methode zur automatisierten Nukleinsäure-Amplifikation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
AU2014312043A1 (en) 2013-08-30 2016-02-25 Illumina France Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
GB2534067B (en) 2013-08-30 2021-07-21 Personalis Inc Methods and systems for genomic analysis
JP6700173B2 (ja) * 2013-09-24 2020-05-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ターゲット検出方法及びシステム
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
GB2535066A (en) 2013-10-03 2016-08-10 Personalis Inc Methods for analyzing genotypes
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
JP6532456B2 (ja) 2013-10-04 2019-06-19 ライフ テクノロジーズ コーポレーション 終止化学を用いる配列決定における整相効果（ｐｈａｓｉｎｇ ｅｆｆｅｃｔｓ）をモデル化するための方法及びシステム
US10294511B2 (en) 2013-10-17 2019-05-21 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
EP3058096A1 (de) 2013-10-18 2016-08-24 Good Start Genetics, Inc. Verfahren zur beurteilung eines genomischen bereichs einer person
CN103540672B (zh) * 2013-10-29 2015-04-08 中国科学技术大学 一种亲和核酸分子的快速鉴定和分离方法
US9897986B2 (en) 2013-10-29 2018-02-20 Regal Beloit America, Inc. System and method for enabling a motor controller to communicate using multiple different communication protocols
US10947587B2 (en) * 2013-11-05 2021-03-16 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
EP4130350A1 (de) 2013-11-07 2023-02-08 The Board of Trustees of the Leland Stanford Junior University Zellfreie nukleinsäuren zur analyse des menschlichen mikrobioms und komponenten davon
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
DK3511422T3 (da) 2013-11-12 2023-02-06 Population Bio Inc Fremgangsmåder og sammensætninger til diagnosticering, prognose og behandling af endometriose
EP3839062A1 (de) 2013-11-12 2021-06-23 Life Technologies Corporation System und verfahren zur emulsionsspaltung
CN104630202A (zh) * 2013-11-13 2015-05-20 北京大学 一种能够减小微量核酸物质整体扩增时产生偏倚的扩增方法
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
US10191071B2 (en) 2013-11-18 2019-01-29 IntegenX, Inc. Cartridges and instruments for sample analysis
CN106460032B (zh) 2013-12-05 2019-12-24 生捷科技控股公司 图案化阵列的制备
US10597715B2 (en) 2013-12-05 2020-03-24 Centrillion Technology Holdings Methods for sequencing nucleic acids
CN105940024B (zh) 2013-12-05 2019-03-15 生捷科技控股公司 修饰的表面
CA2931533C (en) 2013-12-09 2023-08-08 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
CA2933387C (en) 2013-12-11 2023-05-02 The Regents Of The University Of California Methods for labeling dna fragments to reconstruct physical linkage and phase
EP3792921A1 (de) * 2013-12-11 2021-03-17 Genapsys, Inc. Systeme und verfahren für biologische analysen und berechnungen
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015089438A1 (en) 2013-12-13 2015-06-18 Northwestern University Biomarkers for post-traumatic stress states
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
AU2014369841B2 (en) 2013-12-28 2019-01-24 Guardant Health, Inc. Methods and systems for detecting genetic variants
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9387451B2 (en) * 2014-02-03 2016-07-12 International Business Machines Corporation Flow cell array and uses thereof
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US20170292149A1 (en) 2014-03-05 2017-10-12 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
EP3117011B1 (de) 2014-03-13 2020-05-06 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
WO2015141649A1 (ja) * 2014-03-20 2015-09-24 ユニバーサル・バイオ・リサーチ株式会社 核酸増幅自動化装置、及び核酸増幅分析自動化装置
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US20150284715A1 (en) * 2014-04-07 2015-10-08 Qiagen Gmbh Enrichment Methods
DE202015009494U1 (de) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidische Vorrichtungen und Systeme zur Einkapselung und Partitionierung von Reagenzien, und deren Anwendungen
ES2777529T3 (es) 2014-04-17 2020-08-05 Adaptive Biotechnologies Corp Cuantificación de genomas de células inmunitarias adaptativas en una mezcla compleja de células
EP3132060B1 (de) 2014-04-18 2019-03-13 Genapsys Inc. Verfahren und systeme zur nukleinsäureamplifikation
AU2015249846B2 (en) 2014-04-21 2021-07-22 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
EP3151733B1 (de) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Zusammensetzungen und verfahren zur charakterisierung und diagnose von parodontaler erkrankung
US9534215B2 (en) * 2014-06-11 2017-01-03 Life Technologies Corporation Systems and methods for substrate enrichment
US20150361481A1 (en) * 2014-06-13 2015-12-17 Life Technologies Corporation Multiplex nucleic acid amplification
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
CN107209188A (zh) 2014-06-27 2017-09-26 雅培制药有限公司 用于检测人Pegivirus 2 (HPgV‑2)的组合物和方法
EP3160654A4 (de) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-aktivierte sortierung (pas)
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP3169811A4 (de) 2014-07-17 2017-12-27 Celmatix Inc. Verfahren und systeme zur überprüfung von unfruchtbarkeit und verwandten leiden
CN106715718B (zh) 2014-07-24 2021-07-06 雅培分子公司 用于检测和分析结核分枝杆菌的组合物和方法
CA2956925C (en) 2014-08-01 2024-02-13 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
EP3653726B1 (de) 2014-08-11 2021-10-27 Luminex Corporation Sonden für verbesserte schmelzunterscheidung und multiplexierung bei nukleinsäuretests
CA2956723C (en) 2014-08-12 2023-04-11 Nextgen Jane, Inc. System and method for monitoring health based on collected bodily fluid
US10435685B2 (en) * 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
EP3183577B1 (de) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible oberflächenfunktionalisierung
WO2016036403A1 (en) 2014-09-05 2016-03-10 Population Diagnostics Inc. Methods and compositions for inhibiting and treating neurological conditions
EP3191809B1 (de) 2014-09-08 2021-07-07 Becton, Dickinson and Company System und verfahren zur herstellung einer pharmazeutischen verbindung
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
WO2016037361A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 试剂盒及其在核酸测序中的用途
CN105400864B (zh) * 2014-09-12 2020-04-14 深圳华大基因股份有限公司 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途
EP3192869B1 (de) * 2014-09-12 2019-03-27 MGI Tech Co., Ltd. Isoliertes oligonukleotid und verwendung davon bei der nukleinsäuresequenzierung
ES2727656T3 (es) 2014-09-15 2019-10-17 Abvitro Llc Secuenciación de alto rendimiento de banco de nucleótidos
CN107002293B (zh) 2014-09-17 2020-12-08 艾比斯生物科学公司 使用脉冲读数光学的通过合成测序
EP3194627B1 (de) 2014-09-18 2023-08-16 Illumina, Inc. Verfahren und systeme zur analyse von nukleinsäuresequenzierungsdaten
WO2016048829A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
EP3204519B1 (de) 2014-10-10 2020-04-29 Quantapore Inc. Analyse nanoporenbasierter polymere mit gegenseitig löschenden fluoreszenzmarkern
US10676787B2 (en) 2014-10-13 2020-06-09 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
WO2016058121A1 (zh) * 2014-10-13 2016-04-21 深圳华大基因科技有限公司 一种核酸片段化方法和序列组合
EP3209410A4 (de) 2014-10-22 2018-05-02 IntegenX Inc. Systeme und verfahren zur automatisierten vorbereitung, verarbeitung und analyse von proben
EP3209419A4 (de) 2014-10-22 2018-10-03 The Regents of The University of California Hochauflösender mikrotröpfchendrucker
CA2964790C (en) 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
EP3212790B1 (de) 2014-10-29 2020-03-25 Adaptive Biotechnologies Corp. Hochmultiplexierter gleichzeitiger nachweis von für adaptive gepaarte immunrezeptorheterodimere codierenden nukleinsäuren aus einer vielzahl von proben
US10125399B2 (en) 2014-10-30 2018-11-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
ES2772127T3 (es) 2014-10-31 2020-07-07 Illumina Cambridge Ltd Polímeros y recubrimientos de copolímeros de ADN
EP3216852A4 (de) * 2014-11-04 2018-09-26 Toppan Printing Co., Ltd. Verfahren zum einbringen von nukleinsäure, verfahren zur erkennung von nukleinsäure, verfahren zur analyse eines biologischen bestandteils, arrayvorrichtung zum testen eines biologischen bestandteils und kit zur analyse eines biologischen bestandteils
DK3215616T3 (da) 2014-11-05 2020-03-02 Illumina Cambridge Ltd Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer
CN111024936A (zh) 2014-11-05 2020-04-17 纳迈达斯生物科技中心 用于增强成像的金属复合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107206043A (zh) 2014-11-05 2017-09-26 维拉赛特股份有限公司 使用机器学习和高维转录数据在经支气管活检上诊断特发性肺纤维化的系统和方法
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US11789906B2 (en) 2014-11-19 2023-10-17 Arc Bio, Llc Systems and methods for genomic manipulations and analysis
CN107208146B (zh) 2014-11-20 2022-01-18 安普里怀斯公司 用于核酸扩增的组合物和方法
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
US11066705B2 (en) 2014-11-25 2021-07-20 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
JP6905934B2 (ja) 2014-12-05 2021-07-21 ファウンデーション・メディシン・インコーポレイテッド 腫瘍試料の多重遺伝子分析
EP3234198B1 (de) 2014-12-18 2024-04-03 Life Technologies Corporation Kalibrierpanel und verfahren zum entwurf davon
EP3234575B1 (de) 2014-12-18 2023-01-25 Life Technologies Corporation Vorrichtung zur messung von analyten unter verwendung von grossflächigen fet-arrays
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
KR102593647B1 (ko) 2014-12-18 2023-10-26 라이프 테크놀로지스 코포레이션 트랜스미터 구성을 갖춘 높은 데이터율 집적 회로
EP3237616A1 (de) 2014-12-24 2017-11-01 Keygene N.V. Backbone-vermittelte paarsequenzierung
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
SG11201705425SA (en) 2015-01-13 2017-08-30 10X Genomics Inc Systems and methods for visualizing structural variation and phasing information
GB201501012D0 (en) * 2015-01-21 2015-03-04 Base4 Innovation Ltd Improved droplet sequencing apparatus and method
WO2016122582A1 (en) * 2015-01-30 2016-08-04 Hewlett Packard Enterprise Development Lp Relationship preserving projection of digital objects
CN107209120B (zh) 2015-02-02 2020-05-15 株式会社日立高新技术 多色荧光分析装置
JP6307179B2 (ja) * 2015-02-03 2018-04-04 株式会社日立製作所 単一細胞解析用フローセルデバイス及び単一細胞解析装置
CA2974306A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
MX2017010142A (es) 2015-02-09 2017-12-11 10X Genomics Inc Sistemas y metodos para determinar variacion estructural y ajuste de fases con datos de recuperacion de variantes.
JP6755890B2 (ja) * 2015-02-11 2020-09-16 パラゴン ゲノミクス, インク.Paragon Genomics, Inc. 非特異的増幅産物を減少させる為の方法及び組成物
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
CN107250383B (zh) 2015-02-17 2022-09-06 深圳华大智造科技股份有限公司 使用受控的链置换的dna序列测定
CA2976902A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
CN107407685B (zh) 2015-02-20 2021-08-03 宝生物工程（美国)有限公司 快速精确分配、可视化和分析单个细胞的方法
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
AU2016222788B2 (en) 2015-02-24 2022-03-31 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
CN107614096A (zh) 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
CA3225867A1 (en) 2015-03-24 2016-09-29 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
GB2554572B (en) 2015-03-26 2021-06-23 Dovetail Genomics Llc Physical linkage preservation in DNA storage
WO2016160965A1 (en) * 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Methods and compositions for repair of dna ends by multiple enzymatic activities
WO2016160822A1 (en) * 2015-03-30 2016-10-06 Verily Life Sciences Llc Functionalized nanoparticles, methods and in vivo diagnostic system
EP3277294A4 (de) 2015-04-01 2018-11-14 Adaptive Biotechnologies Corp. Verfahren zur identifizierung von menschlichen kompatiblen t-zell-rezeptoren spezifisch für ein antigen-ziel
CN107889508A (zh) 2015-04-02 2018-04-06 杰克逊实验室 使用环化的配对文库和鸟枪测序检测基因组变异的方法
EP3530752B1 (de) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3091026B1 (de) 2015-05-08 2019-02-20 Centrillion Technology Holdings Corporation Disulfidvernetzte reversible terminatoren
ES2826880T3 (es) 2015-05-11 2021-05-19 Illumina Inc Plataforma para el descubrimiento y análisis de agentes terapéuticos
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
EP3295345B1 (de) 2015-05-14 2023-01-25 Life Technologies Corporation Barcodesequenzen sowie zugehörige systeme und verfahren
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
EP3653728B1 (de) 2015-06-09 2023-02-01 Life Technologies Corporation Verfahren, systeme, zusammensetzungen, kits, vorrichtung und computerlesbare medien zur molekularen markierung
WO2016201111A1 (en) 2015-06-09 2016-12-15 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
CN107924121B (zh) 2015-07-07 2021-06-08 亿明达股份有限公司 经由纳米压印的选择性表面图案化
US10526664B2 (en) 2015-07-14 2020-01-07 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
CA2992449A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
CN107835857A (zh) 2015-07-17 2018-03-23 亿明达股份有限公司 用于测序应用的聚合物片层
US10150994B2 (en) 2015-07-22 2018-12-11 Qiagen Waltham, Inc. Modular flow cells and methods of sequencing
JP6712606B2 (ja) * 2015-07-30 2020-06-24 イラミーナ インコーポレーテッド ヌクレオチドのオルトゴナルな脱ブロッキング
JP6946292B2 (ja) 2015-08-06 2021-10-06 エイアールシー バイオ リミテッド ライアビリティ カンパニー ゲノム分析のためのシステムおよび方法
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
US10906044B2 (en) 2015-09-02 2021-02-02 Illumina Cambridge Limited Methods of improving droplet operations in fluidic systems with a filler fluid including a surface regenerative silane
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US9938572B1 (en) 2015-09-08 2018-04-10 Raindance Technologies, Inc. System and method for forming an emulsion
AU2016324296A1 (en) 2015-09-18 2018-04-12 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
CN108289797B (zh) 2015-10-13 2022-01-28 哈佛学院院长及董事 用于制备和使用凝胶微球的系统和方法
AU2016341198B2 (en) 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
WO2017096110A1 (en) * 2015-12-04 2017-06-08 Siemens Healthcare Diagnostics Inc. Method and device for optimizing the process of identification of pathogens
EP3400298B1 (de) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Auflösung mehrerer beads pro tropfen
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
US20190169602A1 (en) 2016-01-12 2019-06-06 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
CN108495938B (zh) * 2016-01-12 2023-07-14 生物辐射实验室股份有限公司 利用相位移区块合成条码化序列及其用途
EP4012416A1 (de) 2016-01-22 2022-06-15 Purdue Research Foundation Verwendung eines markierungssystems mit geladener masse zum nachweis von zielanalyten
JP6685138B2 (ja) 2016-01-27 2020-04-22 シスメックス株式会社 核酸増幅の精度管理方法、精度管理用試薬およびその試薬キット
US10626390B2 (en) 2016-02-08 2020-04-21 RGENE, Inc. Multiple ligase compositions, systems, and methods
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
JP2017143783A (ja) * 2016-02-17 2017-08-24 国立大学法人 筑波大学 並列反応用懸濁液、並列反応方法、スクリーニング方法および検査方法
KR20180116377A (ko) 2016-02-23 2018-10-24 더브테일 제노믹스 엘엘씨 게놈 어셈블리를 위한 페이징된 판독 세트의 생성 및 반수체형 페이징
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US20190062827A1 (en) 2016-03-14 2019-02-28 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
EP3433373B1 (de) 2016-03-22 2022-01-12 Myriad Women's Health, Inc. Kombinatorisches dna-screening
KR102175718B1 (ko) 2016-03-25 2020-11-06 카리우스, 인코포레이티드 합성 핵산 스파이크-인
WO2017172798A1 (en) 2016-03-28 2017-10-05 Illumina, Inc. Multi-plane microarrays
WO2017180909A1 (en) 2016-04-13 2017-10-19 Nextgen Jane, Inc. Sample collection and preservation devices, systems and methods
WO2017180871A1 (en) 2016-04-13 2017-10-19 Purdue Research Foundation Systems and methods for isolating a target in an ion trap
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
CN109415761B (zh) 2016-04-25 2022-09-20 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
CN105821482B (zh) * 2016-04-29 2018-04-10 李星军 一种生化微反应体系、高通量测序的建库仪及应用
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
EP3954771A1 (de) 2016-05-13 2022-02-16 Dovetail Genomics, LLC Rückgewinnung von long-range-bindungsinformationen aus konservierten proben
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN109313396B (zh) 2016-05-18 2022-11-22 Illumina公司 使用图案化疏水性表面进行的自组装图案化
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
WO2017214561A1 (en) 2016-06-10 2017-12-14 Life Technologies Corporation Methods and compositions for nucleic acid amplification
EP3469097B1 (de) * 2016-06-14 2020-02-19 Base4 Innovation Limited Verfahren zur abtrennung eines modifizierten polynukleotids
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
JP7075394B2 (ja) 2016-07-21 2022-05-25 タカラ バイオ ユーエスエー， インコーポレイテッド マルチウェルデバイスを用いたマルチｚ撮像及び分注
WO2018031691A1 (en) 2016-08-10 2018-02-15 The Regents Of The University Of California Combined multiple-displacement amplification and pcr in an emulsion microdroplet
EP3500082A1 (de) 2016-08-22 2019-06-26 Biolumic Limited System, vorrichtung und verfahren zur behandlung von saatgut
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
CN110506127B (zh) 2016-08-24 2024-01-12 维拉科特Sd公司 基因组标签预测前列腺癌患者对术后放射疗法应答性的用途
CN109923216A (zh) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
CN109983125A (zh) 2016-08-31 2019-07-05 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
US20210285038A1 (en) * 2016-08-31 2021-09-16 President And Fellows Of Harvard College Methods of Whole Genome Digital Amplification
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
WO2018049260A1 (en) * 2016-09-09 2018-03-15 Tl Biolabs Corp . Reusable microarray compositions and methods
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
KR20190052132A (ko) 2016-09-27 2019-05-15 유바이오미, 인코포레이티드 크리스퍼-기반 라이브러리 제조 및 서열 분석을 위한 방법 및 시스템
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US10190155B2 (en) * 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
US11725232B2 (en) 2016-10-31 2023-08-15 The Hong Kong University Of Science And Technology Compositions, methods and kits for detection of genetic variants for alzheimer's disease
EP3692161A4 (de) 2016-11-11 2022-05-25 Psomagen, Inc. Verfahren und system zur fragmentanordnung und sequenzidentifizierung
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018118971A1 (en) 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
EP3559269B1 (de) * 2016-12-20 2020-09-16 F. Hoffmann-La Roche AG Einstrangige kreisförmige dna-bibliotheken für kreisförmige konsensussequenzierung
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2017385424A1 (en) * 2016-12-27 2019-08-01 Qingdao MGI Tech Co., Ltd. Single fluorescent dye based sequencing method
EP4112741A1 (de) * 2017-01-04 2023-01-04 MGI Tech Co., Ltd. Schrittweise sequenzierung durch nichtmarkierte reversible terminatoren oder natürliche nukleotide
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
WO2018132459A1 (en) 2017-01-10 2018-07-19 Paragon Genomics, Inc. Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions
US11208697B2 (en) 2017-01-20 2021-12-28 Decipher Biosciences, Inc. Molecular subtyping, prognosis, and treatment of bladder cancer
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
US10240205B2 (en) 2017-02-03 2019-03-26 Population Bio, Inc. Methods for assessing risk of developing a viral disease using a genetic test
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018156418A1 (en) 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
EP3838268B1 (de) 2017-02-24 2023-05-10 The Regents of the University of California Partikeltropfenstrukturen und verfahren zur herstellung und verwendung davon
GB201703049D0 (en) 2017-02-24 2017-04-12 Univ I Tromsø - Norges Arktiske Univ Single-strand binding protein
JP6931540B2 (ja) 2017-02-27 2021-09-08 シスメックス株式会社 検体処理チップを用いた送液方法、検体処理チップの送液装置
EP3593140A4 (de) 2017-03-09 2021-01-06 Decipher Biosciences, Inc. Subtypisierung von prostatakrebs zur vorhersage der reaktion auf eine hormontherapie
EP3595674A4 (de) 2017-03-15 2020-12-16 Twist Bioscience Corporation Variante bibliotheken der immunologischen synapse und synthese davon
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
FI3607065T3 (fi) * 2017-04-06 2023-06-16 Qing Wang Menetelmä ja tarvikesarja nukleiinihappokirjaston rakentamista varten
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
US20210371918A1 (en) 2017-04-18 2021-12-02 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
JP2018183097A (ja) * 2017-04-26 2018-11-22 株式会社エンプラス 細胞由来の解析用液滴の単離方法、および細胞の解析方法
CA3062716A1 (en) 2017-05-12 2018-11-15 Decipher Biosciences, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor agressiveness
CN106957842B (zh) * 2017-05-15 2020-05-22 青岛安德贝生命科技有限公司 Bac克隆dna的提取方法
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
WO2018222800A2 (en) * 2017-05-31 2018-12-06 Centrillion Technologies, Inc. Oligonucleotide probe array with electronic detection system
EP3634992B1 (de) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Verfahren und zusammensetzungen zur identifizierung von epitopen
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11001885B2 (en) * 2017-06-13 2021-05-11 Personal Genomics Taiwan, Inc. Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
US20200131506A1 (en) 2017-06-21 2020-04-30 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
US11725305B2 (en) * 2017-07-17 2023-08-15 SeqOnce Biosciences, Inc. Rapid library construction for high throughput sequencing
EP3668651B1 (de) 2017-08-15 2024-05-01 Pacific Biosciences of California, Inc. Verfahren zur sequenzierung von nukleinsäuren unter verwendung einer abtastvorrichtung
WO2019038594A2 (en) 2017-08-21 2019-02-28 Biolumic Limited TRANSGENIC PLANTS WITH HIGH GROWTH AND HIGH RUSTICITY
WO2019040788A1 (en) 2017-08-24 2019-02-28 Takara Bio Usa, Inc. METHODS FOR PRODUCING NUCLEIC ACIDS USING STIMULUS-MODIFIED OLIGONUCLEOTIDES
EP3681906A4 (de) 2017-09-11 2021-06-09 Twist Bioscience Corporation Gpcr-bindende proteine und deren synthese
CN116850284A (zh) 2017-09-20 2023-10-10 瑞泽恩制药公司 用于其肿瘤携带高过客基因突变负荷的患者的免疫治疗方法
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
AU2018401700A1 (en) * 2017-09-29 2020-04-09 The Regents Of The University Of California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
AU2018346765A1 (en) 2017-10-06 2020-04-16 Oncotherapy Science, Inc. Screening of T lymphocytes for cancer-specific antigens
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. BACKGROUND NOISE REDUCTION AND STABILIZATION OF SIMULTANEOUS COMPLEXES IN LINKED ASSAY WORKFLOWS
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (de) 2017-10-27 2022-12-28 10X Genomics, Inc. Verfahren zur probenvorbereitung und -analyse
CN111295443B (zh) 2017-11-02 2024-04-16 生物辐射实验室股份有限公司 基于转座酶的基因组分析
EP3625361A1 (de) 2017-11-15 2020-03-25 10X Genomics, Inc. Funktionalisierte gelperlen
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CA3083183A1 (en) 2017-11-22 2019-05-31 The Regents Of The University Of Michigan Compositions and methods for treating cancer
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
US11427867B2 (en) 2017-11-29 2022-08-30 Xgenomes Corp. Sequencing by emergence
EP3717645A4 (de) 2017-11-29 2021-09-01 XGenomes Corp. Sequenzierung von nukleinsäuren durch austritt
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
JP2021509024A (ja) 2018-01-02 2021-03-18 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 多数の液滴の捕捉
KR20200106067A (ko) 2018-01-04 2020-09-10 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
EP3738122A1 (de) 2018-01-12 2020-11-18 Life Technologies Corporation Verfahren zur vorhersage der strömungsqualitätsbewertung durch neuronale netze
WO2019152543A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
EP3746552B1 (de) 2018-01-31 2023-12-27 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur dekonvolution von partitionsbarcodes
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
CN110184325A (zh) * 2018-02-22 2019-08-30 张家港万众一芯生物科技有限公司 基于微孔阵列芯片的单分子文库pcr扩增的基因测序方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
AU2019235867A1 (en) * 2018-03-13 2020-10-29 Sarmal, Inc. Methods for single molecule sequencing
CA3095292A1 (en) 2018-04-02 2019-10-10 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
EP3775271A1 (de) 2018-04-06 2021-02-17 10X Genomics, Inc. Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung
EP3553182A1 (de) 2018-04-11 2019-10-16 Université de Bourgogne Verfahren zum nachweis von somatischen genetischen anomalien, kombination von fangsonden und nachweiskit
WO2019203986A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP2021521820A (ja) 2018-04-26 2021-08-30 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸−ヌクレオチド−ポリメラーゼ複合体を安定化するための方法及び組成物
US20210239700A1 (en) 2018-05-04 2021-08-05 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
CN108588200A (zh) * 2018-05-06 2018-09-28 湖南大地同年生物科技有限公司 一种R-Loop高通量测序文库构建方法
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
EP3802874A1 (de) 2018-05-31 2021-04-14 Omniome, Inc. Erhöhtes signal-rausch-verhältnis bei der sequenzierung von nukleinsäuren
US11180794B2 (en) * 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112770776A (zh) 2018-07-30 2021-05-07 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
FI3625368T3 (fi) 2018-08-08 2023-01-13 Menetelmiä john cunningham -viruksen aiheuttaman progressiivin multifokaalin leukoenkefalopatian kehittymisen riskin arviointiin geneettisellä testauksella
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020069424A1 (en) 2018-09-28 2020-04-02 Centrillion Technologies, Inc. Disulfide-linked reversible terminators
CN112840023B (zh) 2018-10-25 2024-03-01 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
EP3887545A4 (de) * 2018-11-29 2022-08-24 XGenomes Corp. Sequenzierung durch koalaszenz
CN211311477U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 测序系统
US11354926B2 (en) 2018-12-07 2022-06-07 Bfly Operations, Inc. Ultrasound fingerprint detection and related apparatus and methods
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
KR20210104779A (ko) 2018-12-13 2021-08-25 디엔에이 스크립트 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성
WO2020132103A1 (en) * 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
CN109738469A (zh) * 2018-12-29 2019-05-10 赛纳生物科技(北京)有限公司 一种fop表面微坑镀膜的致密性检测方法
US20220081714A1 (en) 2019-01-04 2022-03-17 Northwestern University Storing temporal data into dna
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020146740A1 (en) 2019-01-10 2020-07-16 Iovance Biotherapeutics, Inc. System and methods for monitoring adoptive cell therapy clonality and persistence
KR20210138017A (ko) * 2019-02-11 2021-11-18 울티마 제노믹스, 인크. 핵산 분석 방법
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (zh) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 用于检测化学和生物分析物的扫描装置和方法
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
KR20210143766A (ko) 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
JP2022525322A (ja) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
EP3947718A4 (de) 2019-04-02 2022-12-21 Enumera Molecular, Inc. Verfahren, systeme und zusammensetzungen zum zählen von nukleinsäuremolekülen
US20220179891A1 (en) * 2019-04-09 2022-06-09 University Of Washington Systems and methods for providing similarity-based retrieval of information stored in dna
CN109853047A (zh) * 2019-04-10 2019-06-07 翌圣生物科技（上海）有限公司 一种基因组dna测序文库快速构建方法及配套试剂盒
AU2020266530A1 (en) 2019-04-29 2021-04-08 Illumina, Inc. Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
US20210017596A1 (en) * 2019-05-08 2021-01-21 Qiagen Sciences, Llc Sequential sequencing methods and compositions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
MA56037A (fr) 2019-05-28 2022-04-06 Octant Inc Système de relais de transcription
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
CN110499361B (zh) * 2019-07-31 2022-11-25 齐鲁工业大学 一种末端碱基流式荧光测序微球的制备方法及应用
AU2020333348B2 (en) 2019-08-19 2023-11-23 Green Cross Genome Corporation Method for detecting chromosomal abnormality by using information about distance between nucleic acid fragments
EP4028407B1 (de) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible nukleotide änderung
BR112021026562A2 (pt) 2019-09-20 2022-11-29 Illumina Inc Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras
WO2021072306A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
KR20220094193A (ko) 2019-11-07 2022-07-05 온세르나 테라퓨틱스, 인크. 종양 미세환경의 분류
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
CN110951852B (zh) * 2019-11-25 2022-11-25 齐鲁工业大学 单碱基连续延伸流式靶向测序法
AU2020391556B2 (en) 2019-11-29 2024-01-04 GC Genome Corporation Artificial intelligence-based chromosomal abnormality detection method
EP3891300B1 (de) 2019-12-23 2023-03-29 10X Genomics, Inc. Verfahren zur räumlichen analyse mittels rna-template-ligation
EP4090328A4 (de) 2020-01-13 2024-02-14 Fluent Biosciences Inc Wirkstoff-screening auf emulsionsbasis
JP2023511278A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞遺伝子プロファイリングのための方法およびシステム
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US20230054204A1 (en) 2020-02-04 2023-02-23 Pacific Biosciences Of California, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
IL294909A (en) 2020-02-13 2022-09-01 Zymergen Inc A metagenomic library and natural product discovery platform
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
CN111455469B (zh) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4146822A1 (de) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Zusammensetzungen und verfahren zur modifizierung von polymerase-nukleinsäurekomplexen
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021281702A1 (en) 2020-05-27 2023-01-05 Genetic Technologies Limited Methods of assessing risk of developing a severe response to Coronavirus infection
WO2021247568A1 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Spatial trancriptomics for antigen-receptors
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4162074B1 (de) 2020-06-08 2024-04-24 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
WO2022008578A1 (en) 2020-07-08 2022-01-13 F. Hoffmann-La Roche Ag Targeted depletion of non-target library molecules using poison primers during target capture of next-generation sequencing libraries
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CA3200519A1 (en) * 2020-11-03 2022-05-12 Fluent Biosciences Inc. Methods and systems for detecting pathogenic microbes in a patient
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
KR20220074088A (ko) 2020-11-27 2022-06-03 주식회사 지씨지놈 인공지능 기반 암 진단 및 암 종 예측방법
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022155331A1 (en) 2021-01-13 2022-07-21 Pacific Biosciences Of California, Inc. Surface structuring with colloidal assembly
US20240043915A1 (en) 2021-02-13 2024-02-08 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
CN117222747A (zh) 2021-03-22 2023-12-12 伊鲁米纳剑桥有限公司 用于改善核酸簇克隆性的方法
WO2022208171A1 (en) 2021-03-31 2022-10-06 UCL Business Ltd. Methods for analyte detection
US20220336052A1 (en) * 2021-04-19 2022-10-20 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
KR20220160806A (ko) 2021-05-28 2022-12-06 주식회사 지씨지놈 세포유리 핵산단편 말단 서열 모티프 빈도 및 크기를 이용한 암 진단 및 암 종 예측방법
CN117677435A (zh) 2021-06-15 2024-03-08 伊鲁米纳公司 用于测序的不含水凝胶的表面官能化
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CN113174388B (zh) * 2021-06-28 2021-09-10 中国农业大学 一种功能核酸纳米杆与功能核酸纳米花的制备及形貌转换方法
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
WO2023002203A1 (en) 2021-07-21 2023-01-26 Dnae Diagnostics Limited Method and system comprising a cartridge for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
CN117813391A (zh) 2021-07-23 2024-04-02 因美纳有限公司 制备用于dna测序的基底表面的方法
EP4196605A1 (de) 2021-09-01 2023-06-21 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023081300A2 (en) * 2021-11-03 2023-05-11 Abbott Laboratories Systems and methods for sample analysis
US20230348957A1 (en) 2021-12-07 2023-11-02 Luminex Corporation Methods and compositions for nucleic acid analysis
WO2023114190A1 (en) * 2021-12-13 2023-06-22 Cz Biohub Sf, Inc. Single-cell epigenomic profiling using fluidics and hydrogels
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023135485A1 (en) 2022-01-13 2023-07-20 Oslo Universitetssykehus Hf Prostate cancer markers and uses thereof
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023152568A2 (en) 2022-02-10 2023-08-17 Oslo Universitetssykehus Hf Compositions and methods for characterizing lung cancer
AU2023234670A1 (en) * 2022-03-15 2024-01-18 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
US20230343414A1 (en) 2022-03-25 2023-10-26 Illumina, Inc. Sequence-to-sequence base calling
CN114592023B (zh) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 一种细胞裂解自组装多肽复合物、自组装方法、自组装多肽制剂及应用
EP4253550A1 (de) 2022-04-01 2023-10-04 GenCC GmbH 6 Co. KG Verfahren zur herstellung eines viralen, eines vektoriellen oder eines beliebigen transportsystems und krebsspezifischer crispr-komplexe
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US20230392201A1 (en) 2022-06-06 2023-12-07 Element Biosciences, Inc. Methods for assembling and reading nucleic acid sequences from mixed populations
WO2023239917A1 (en) 2022-06-09 2023-12-14 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2024050450A1 (en) 2022-08-31 2024-03-07 Gigamune, Inc. Engineered enveloped vectors and methods of use thereof
WO2024072614A1 (en) 2022-09-27 2024-04-04 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification

Family Cites Families (235)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4175054A (en) 1976-11-11 1979-11-20 Petrolite Corporation Use of hydrocarbon polymers in demulsification
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5171534A (en) * 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801529A (en) 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US4822746A (en) * 1986-06-25 1989-04-18 Trustees Of Tufts College Radiative and non-radiative energy transfer and absorbance modulated fluorescence detection methods and sensors
US5252494A (en) * 1986-06-25 1993-10-12 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix
US5254477A (en) * 1986-06-25 1993-10-19 Trustees Of Tufts College Flourescence intramolecular energy transfer conjugate compositions and detection methods
US5143853A (en) * 1986-06-25 1992-09-01 Trustees Of Tufts College Absorbance modulated fluorescence detection methods and sensors
US4994372A (en) * 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5525464A (en) * 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
DE68908054T2 (de) 1988-01-21 1994-03-10 Genentech Inc Verstärkung und nachweis von nukleinsäuresequenzen.
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4971903A (en) * 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US5225332A (en) * 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
WO1989010566A1 (en) 1988-04-22 1989-11-02 Massachusetts Institute Of Technology Process for forming and using microdroplets
US5700637A (en) * 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US6054270A (en) * 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US4938876A (en) 1989-03-02 1990-07-03 Ohsol Ernest O Method for separating oil and water emulsions
US5629158A (en) * 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
JPH02299598A (ja) 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5871928A (en) * 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
WO1991005058A1 (en) 1989-10-05 1991-04-18 Glenn Kawasaki Cell-free synthesis and isolation of novel genes and polypeptides
DK0515506T3 (da) * 1990-02-16 2000-05-08 Hoffmann La Roche Fremgangsmåde til påvisning af carcinogene humane papillomavirus
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
JPH04223A (ja) * 1990-04-16 1992-01-06 Toshiba Corp 無線電話装置、その充電方法および無線電話充電システム
DE69131962T2 (de) 1990-10-11 2000-07-27 Advanced Res & Tech Inst Verfahren und vorrichtung für die fragmentation von biomaterialien
US5506100A (en) * 1990-10-11 1996-04-09 Indiana University Foundation Process and apparatus for fragmenting biomaterials
US5320814A (en) * 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5250264A (en) * 1991-01-25 1993-10-05 Trustees Of Tufts College Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
US5244636A (en) * 1991-01-25 1993-09-14 Trustees Of Tufts College Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample
US5114984A (en) * 1991-04-26 1992-05-19 Olin Corporation Process for producing an antimicrobially effective polyurethane
JPH06509473A (ja) * 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
AU2674092A (en) * 1991-09-09 1993-04-05 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
EP0969102B1 (de) * 1991-09-24 2007-12-26 Keygene N.V. Primer, Sätze und Restriktions Fragmente und dessen Benutzung in selektive Restriktionsfragmentenamplifikation
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
CA2124087C (en) * 1991-11-22 2002-10-01 James L. Winkler Combinatorial strategies for polymer synthesis
US5445971A (en) * 1992-03-20 1995-08-29 Abbott Laboratories Magnetically assisted binding assays using magnetically labeled binding members
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
DE4223169C1 (de) 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Verfahren zur Mikroverkapselung wasserlöslicher Wirkstoffe
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
US6114114A (en) * 1992-07-17 2000-09-05 Incyte Pharmaceuticals, Inc. Comparative gene transcript analysis
RU2048522C1 (ru) 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5298741A (en) * 1993-01-13 1994-03-29 Trustees Of Tufts College Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample
IL104384A (en) 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
JPH08506664A (ja) 1993-02-01 1996-07-16 セック，リミテッド Ｄｎａ配列決定の方法および装置
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
EP0696200A4 (de) 1993-04-19 1998-04-15 Medisorb Technologies Internat Die umhüllung von nukleinsäuren mit konjugaten, die die aufnahme in zielzellen und die gen-expression erleichtern
EP0695368A4 (de) 1993-04-19 1996-05-08 Stuart A Kauffman Zufallsprozess für die chemische darstellung neuer verbindungen
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5482845A (en) * 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5429807A (en) * 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
DK0725682T3 (da) * 1993-10-28 2002-07-15 Houston Advanced Res Ct Mikrofremstillet, porøst gennemløbsapparat til diskret påvisning af bindingsreaktioner
AU8124694A (en) 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
GB9401833D0 (en) * 1994-02-01 1994-03-30 Isis Innovation Method for discovering ligands
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
WO1995024929A2 (en) 1994-03-15 1995-09-21 Brown University Research Foundation Polymeric gene delivery system
AU2187295A (en) * 1994-03-18 1995-10-09 General Hospital Corporation, The Cleaved amplified rflp detection methods
JP2556293B2 (ja) * 1994-06-09 1996-11-20 日本電気株式会社 Ｍｏｓ ｏｔａ
US5512490A (en) * 1994-08-11 1996-04-30 Trustees Of Tufts College Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5795716A (en) * 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5919673A (en) * 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
US5707807A (en) * 1995-03-28 1998-01-13 Research Development Corporation Of Japan Molecular indexing for expressed gene analysis
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
JPH11504218A (ja) 1995-04-24 1999-04-20 クロマゾーム コーポレーション 新規代謝経路の生成およびスクリーニングのための方法
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US5690894A (en) * 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5910408A (en) 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US5814444A (en) * 1995-06-07 1998-09-29 University Of Washington Methods for making and using single-chromosome amplfication libraries
EP0748860B1 (de) * 1995-06-14 2001-08-29 Tonen Corporation Emulsionsspaltung durch Mikroorganismen
US5728529A (en) * 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US6200737B1 (en) * 1995-08-24 2001-03-13 Trustees Of Tufts College Photodeposition method for fabricating a three-dimensional, patterned polymer microstructure
US5843655A (en) * 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US5871697A (en) * 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
US5962228A (en) * 1995-11-17 1999-10-05 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ATE199572T1 (de) 1995-11-21 2001-03-15 Univ Yale Unimolekulare segmentamplifikation und bestimmung
US5633972A (en) * 1995-11-29 1997-05-27 Trustees Of Tufts College Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
US5814524A (en) * 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
US5851772A (en) * 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
WO1997029211A1 (en) * 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
EP0886681A1 (de) * 1996-03-04 1998-12-30 Genetrace Systems, Inc. Verfahren zur suche von nukleinsäuren mit massenspektroskopie
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US5770637A (en) * 1996-05-01 1998-06-23 Johnson & Johnson Vision Products, Inc. Anti-bacterial, UV absorbable, tinted, metal-chelating polymers
US6022688A (en) * 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US5846727A (en) * 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
PL331513A1 (en) * 1996-06-06 1999-07-19 Lynx Therapeutics Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US5916524A (en) * 1997-07-23 1999-06-29 Bio-Dot, Inc. Dispensing apparatus having improved dynamic range
US5846721A (en) * 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
AU4596597A (en) 1996-09-27 1998-04-17 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6887665B2 (en) * 1996-11-14 2005-05-03 Affymetrix, Inc. Methods of array synthesis
DE19648372A1 (de) 1996-11-22 1998-05-28 Serck Como Gmbh Verfahren und Vorrichtung zum Eindampfen von Abwässern
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6310354B1 (en) 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
US6060245A (en) * 1996-12-13 2000-05-09 Stratagene Methods and adaptors for generating specific nucleic acid populations
US20020172965A1 (en) * 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
DK0971946T3 (da) 1997-01-21 2006-10-30 Gen Hospital Corp Selektion af proteiner ved anvendelse af RNA-protein-fusioner
WO1998033939A1 (fr) * 1997-01-31 1998-08-06 Hitachi, Ltd. Procede pour determiner une sequence de base d'acide nucleique et appareil correspondant
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
CA2281205A1 (en) * 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US5994068A (en) * 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
EP0975969A4 (de) 1997-03-18 2002-07-31 Chromaxome Corp Screening-methode für verbindungen mit hilfe eingekapselter zellen
US5891477A (en) 1997-03-28 1999-04-06 Biohybrid Technologies, Inc. Non-steroidal anti-inflammatory agents inhibition of fibrotic response to an implanted device
EP3034626A1 (de) * 1997-04-01 2016-06-22 Illumina Cambridge Limited Verfahren zur vervielfältigung von nukleinsäuren
AU6846798A (en) * 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid sequencing
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6406845B1 (en) 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
DE69801749T2 (de) * 1997-05-13 2002-07-04 Azign Bioscience As Kopenhagen Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)
ATE358177T1 (de) * 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
JP3610231B2 (ja) * 1997-08-01 2005-01-12 キヤノン株式会社 反応場アレー、反応場アレーの製造方法、反応場アレーを用いた反応方法及び反応場アレーを用いた試料溶液中の物質の定量方法
ATE443866T1 (de) 1997-08-01 2009-10-15 Canon Kk Reaktionsstellenarray, verfahren zu seiner herstellung, reaktionsverfahren unter seiner verwendung und quantitatives bestimmungsverfahren für eine substanz in einer probelösung unter seiner verwendung
US5961228A (en) * 1997-08-22 1999-10-05 Paxar Corporation Modular printer
US20010006630A1 (en) * 1997-09-02 2001-07-05 Oron Yacoby-Zeevi Introducing a biological material into a patient
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6475722B1 (en) 1997-12-03 2002-11-05 Curagen Corporation Surface treatments for DNA processing devices
NL1007781C2 (nl) 1997-12-12 1999-06-15 Packard Instr Bv Microtiterplaat.
WO1999032660A1 (en) 1997-12-19 1999-07-01 Affymetrix Exploiting genomics in the search for new drugs
EP1600512B1 (de) 1997-12-22 2008-05-28 Hitachi Chemical Co., Ltd. Reinigung von mRNA auf Mikroplatten
KR100280219B1 (ko) * 1998-02-26 2001-04-02 이수빈 삼핵산 반복 서열을 이용한 신경정신 질환의 진단 방법 및 진단 시약
US5882874A (en) * 1998-02-27 1999-03-16 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6378527B1 (en) 1998-04-08 2002-04-30 Chondros, Inc. Cell-culture and polymer constructs
AU3572799A (en) * 1998-04-24 1999-11-16 Genova Pharmaceuticals Corporation Function-based gene discovery
JP2981547B1 (ja) 1998-07-02 1999-11-22 農林水産省食品総合研究所長 クロスフロー型マイクロチャネル装置及び同装置を用いたエマルションの生成または分離方法
EP1100889A4 (de) 1998-07-17 2002-03-06 Mirus Corp Micell systeme
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
CA2247545C (en) 1998-09-15 2008-12-30 Dynal As Method of isolation of primer extension products with modular oligonucleotides
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6203989B1 (en) * 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
WO2000039343A1 (en) * 1998-12-31 2000-07-06 City Of Hope Method for detecting mutations in nucleic acids
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US20020150909A1 (en) 1999-02-09 2002-10-17 Stuelpnagel John R. Automated information processing in randomly ordered arrays
US6255476B1 (en) * 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
WO2000050632A2 (en) * 1999-02-22 2000-08-31 Lynx Therapeutics, Inc. Polymorphic dna fragments and uses thereof
AU3174600A (en) * 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
US6225061B1 (en) * 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
JP2004512807A (ja) * 1999-03-18 2004-04-30 コンプリート　ゲノミックス　エイエス 可読情報を有するフラグメント鎖のクローニングおよび生成方法
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
US20030108867A1 (en) * 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
CA2370976C (en) 1999-04-20 2009-10-20 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6221653B1 (en) * 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6620584B1 (en) 1999-05-20 2003-09-16 Illumina Combinatorial decoding of random nucleic acid arrays
US20020051971A1 (en) * 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6300070B1 (en) * 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6340589B1 (en) 1999-07-23 2002-01-22 Mj Research, Inc. Thin-well microplate and methods of making same
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
AU2438501A (en) 1999-12-21 2001-07-03 Government Of The United States Of America, As Represented By The Secretary Of The Navy, The Expression of proteins from amplified, immobilized nucleic acids
EP1255860A2 (de) * 1999-12-29 2002-11-13 Mergen Ltd. Festphasen multiplex amplifizierung von polynukleotiden
CA2398690A1 (en) 2000-01-20 2001-07-26 Rosetta Inpharmatics, Inc. Barcoded synthetic lethal screening to identify drug targets
DE60127939T2 (de) 2000-02-07 2008-01-24 Illumina, Inc., San Diego Nukleinsäure-Nachweisverfahren mit universellem Priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6714874B1 (en) 2000-03-15 2004-03-30 Applera Corporation Method and system for the assembly of a whole genome using a shot-gun data set
JP3442338B2 (ja) 2000-03-17 2003-09-02 株式会社日立製作所 Ｄｎａ分析装置、ｄｎａ塩基配列決定装置、ｄｎａ塩基配列決定方法、および反応モジュール
CN1189159C (zh) 2000-05-05 2005-02-16 欧莱雅 含水溶性美容活性组分水性核的微胶囊及含其的组合物
US6475736B1 (en) * 2000-05-23 2002-11-05 Variagenics, Inc. Methods for genetic analysis of DNA using biased amplification of polymorphic sites
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
EP1379995A2 (de) 2000-08-09 2004-01-14 Illumina, Inc. Automatisierte informationsverarbeitung mit frei wählbar angeordneten matrizen
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0022069D0 (en) * 2000-09-08 2000-10-25 Pyrosequencing Ab Method
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
AU2001293163A1 (en) * 2000-09-27 2002-04-08 Lynx Therapeutics, Inc. Method for determining relative abundance of nucleic acid sequences
US6632610B2 (en) 2000-10-12 2003-10-14 Gensat S.A. Methods of identification and isolation of polynucleotides containing nucleic acid differences
US20020172980A1 (en) * 2000-11-27 2002-11-21 Phan Brigitte Chau Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems
JP2002257070A (ja) * 2001-02-28 2002-09-11 Toyota Industries Corp 真空ポンプにおける軸封構造
US6936264B2 (en) 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
US6996287B1 (en) 2001-04-20 2006-02-07 Adobe Systems, Inc. Method and apparatus for texture cloning
GB0114854D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
US20050009022A1 (en) * 2001-07-06 2005-01-13 Weiner Michael P. Method for isolation of independent, parallel chemical micro-reactions using a porous filter
US20030064400A1 (en) 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
US6902921B2 (en) * 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
CA2466821A1 (en) 2001-11-21 2003-06-05 Applera Corporation Digital assay
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
CA2481495C (en) * 2002-04-04 2011-08-30 Biotage Ab Primer extension based method employing nucleotides labelled via cleavable linkers
JP4395133B2 (ja) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク． Ｄｎａの単一分子増幅および検出
AU2004206206A1 (en) * 2003-01-15 2004-08-05 Wyeth Novel high throughput method of generating and purifying labeled cRNA targets for gene expression analysis
ES2342665T3 (es) 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
JP5183063B2 (ja) * 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
CA2553833C (en) 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
US7682816B2 (en) 2005-04-07 2010-03-23 454 Life Sciences Corporation Thin film coated microwell arrays and methods of using same
US7785862B2 (en) 2005-04-07 2010-08-31 454 Life Sciences Corporation Thin film coated microwell arrays
EP1910537A1 (de) 2005-06-06 2008-04-16 454 Life Sciences Corporation Sequenzierung mit gepaarten enden
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
JP5808515B2 (ja) 2006-02-16 2015-11-10 ４５４ ライフ サイエンシーズ コーポレイション 核酸配列データのプライマー伸長誤差を補正するためのシステムおよび方法
WO2007149432A2 (en) 2006-06-19 2007-12-27 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
US7585865B2 (en) 2006-07-21 2009-09-08 The Penn State Research Foundation Protein kinase C zeta inhibition to treat vascular permeability
JP2010521156A (ja) 2007-03-16 2010-06-24 ４５４ ライフ サイエンシーズ コーポレイション Ｈｉｖ薬物耐性バリアントの検出のためのシステムおよび方法
ES2774725T3 (es) 2017-05-16 2020-07-22 Arkema France Método de fabricación de 1,4-bis(4-fenoxibenzoil)benceno en condiciones de sobresaturación

Also Published As

Publication number Publication date
JP2012231799A (ja) 2012-11-29
JP5726155B2 (ja) 2015-05-27
WO2004069849A3 (en) 2005-03-24
AU2004209426A1 (en) 2004-08-19
US20090011959A1 (en) 2009-01-08
CN102212614A (zh) 2011-10-12
AU2004209001A1 (en) 2004-08-19
EP1590477A4 (de) 2008-04-09
ES2338654T5 (es) 2017-12-11
US20160298175A1 (en) 2016-10-13
EP1590477A2 (de) 2005-11-02
CA2513899A1 (en) 2005-01-13
CA2728746A1 (en) 2005-01-13
US20090048124A1 (en) 2009-02-19
DE602004024034D1 (de) 2009-12-24
JP2013090635A (ja) 2013-05-16
CN102212614B (zh) 2013-12-25
US7244567B2 (en) 2007-07-17
EP2261372B1 (de) 2012-08-22
CA2513899C (en) 2013-03-26
JP5258822B2 (ja) 2013-08-07
WO2004069849A8 (en) 2007-04-19
JP2006515522A (ja) 2006-06-01
US20190203263A1 (en) 2019-07-04
ES2338654T3 (es) 2010-05-11
US7323305B2 (en) 2008-01-29
US20140162885A1 (en) 2014-06-12
JP2007523627A (ja) 2007-08-23
US10240192B2 (en) 2019-03-26
JP2007535892A (ja) 2007-12-13
JP2013066475A (ja) 2013-04-18
ES2396245T3 (es) 2013-02-20
CA2727850A1 (en) 2005-01-13
CA2513535A1 (en) 2004-08-19
CN101128601B (zh) 2011-06-08
CA2513889A1 (en) 2004-08-19
EP2145955B1 (de) 2012-02-22
AU2004254552B2 (en) 2008-04-24
ES2342665T3 (es) 2010-07-12
WO2004070005A2 (en) 2004-08-19
EP1594981A4 (de) 2008-03-05
EP2145955A3 (de) 2011-03-30
WO2005003375A3 (en) 2007-08-23
WO2004070007A3 (en) 2005-12-22
WO2004069849A2 (en) 2004-08-19
ATE437945T1 (de) 2009-08-15
WO2004070007A2 (en) 2004-08-19
JP4768598B2 (ja) 2011-09-07
JP2010142233A (ja) 2010-07-01
EP1594980A2 (de) 2005-11-16
JP5495399B2 (ja) 2014-05-21
EP2159285B1 (de) 2012-09-26
JP4480715B2 (ja) 2010-06-16
ATE448302T1 (de) 2009-11-15
EP1594980B2 (de) 2017-08-16
US20150099672A1 (en) 2015-04-09
EP1594981B1 (de) 2010-03-17
ATE461291T1 (de) 2010-04-15
EP1594950A4 (de) 2007-07-25
US8158359B2 (en) 2012-04-17
EP1590477B1 (de) 2009-07-29
EP1997889A2 (de) 2008-12-03
CA2513535C (en) 2012-06-12
EP2145955A2 (de) 2010-01-20
AU2004209001B2 (en) 2007-10-11
EP2159285A2 (de) 2010-03-03
US20050130173A1 (en) 2005-06-16
US20130078638A1 (en) 2013-03-28
JP2007525151A (ja) 2007-09-06
US20040185484A1 (en) 2004-09-23
DE602004022253D1 (de) 2009-09-10
AU2004209416A1 (en) 2004-08-19
US20050079510A1 (en) 2005-04-14
ES2330339T3 (es) 2009-12-09
EP2159285A3 (de) 2011-06-29
US8012690B2 (en) 2011-09-06
JP4473878B2 (ja) 2010-06-02
WO2004070005A3 (en) 2004-11-11
US10982274B2 (en) 2021-04-20
EP1594981A2 (de) 2005-11-16
WO2004070005B1 (en) 2005-01-20
US8748102B2 (en) 2014-06-10
EP1594980B1 (de) 2009-11-11
WO2005003375A2 (en) 2005-01-13
DE602004036672C5 (de) 2012-11-29
US20110201526A1 (en) 2011-08-18
EP1997889A3 (de) 2009-09-23
AU2004209416B2 (en) 2007-08-02
EP1594950A2 (de) 2005-11-16
US8790876B2 (en) 2014-07-29
AU2004209426B2 (en) 2008-04-17
US20060134633A1 (en) 2006-06-22
US20110009275A1 (en) 2011-01-13
CN101128601A (zh) 2008-02-20
AU2004254552A1 (en) 2005-01-13
EP2261372A2 (de) 2010-12-15
ES2380893T3 (es) 2012-05-21
CA2728746C (en) 2018-01-16
US20080132693A1 (en) 2008-06-05
JP2010178744A (ja) 2010-08-19
CA2513541A1 (en) 2004-08-19
ATE546525T1 (de) 2012-03-15
CA2727850C (en) 2013-04-30
US20120238475A1 (en) 2012-09-20
US8765380B2 (en) 2014-07-01
US7842457B2 (en) 2010-11-30
EP1594980A4 (de) 2007-05-02
EP2261372A3 (de) 2011-04-06

Similar Documents

Publication Publication Date Title
DE602004026033D1 (de) Zweiendige sequenzierung
WO2007086935A3 (en) Methods of amplifying and sequencing nucleic acids
WO2005116257A3 (en) Unique short tandem repeats and methods of their use
WO2004051218A8 (en) Multiplex amplification of polynucleotides
WO2003064691A3 (en) Methods and means for amplifying nucleic acid
WO2007024798A3 (en) Apparatus, system, and method using immiscible-fluid-discrete-volumes
DE60226500D1 (en) Late-pcr
WO2007066831A3 (en) Method of detecting human papilloma virus by using nucleic acid amplification method and nucleic acid chain-immobilized carrier
WO2002090505A3 (en) Multiple sequencible and ligatible structures for genomic analysis
WO2006077102A3 (en) Method for the detection of hpv and probes, primers and kits
DK1723256T3 (da) CpG-Amplikon og array-kontrol
EP2006393A4 (de) Verfahren zur analyse von nukleinsäuren
WO2005100611A3 (en) Detection of viral nucleic acid and method for reverse transcribing rna
AU2002218129A1 (en) Method for the detection of cytosine methylations
DE502006005960D1 (de) Polymerase-Kettenreaktionsverfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaften
WO2006039673A3 (en) Primers, methods and kits for amplifying or detecting human leukocyte antigen alleles
GB0300802D0 (en) Method for detecting DNA polymerisation
AU2003266255A1 (en) Method for amplification of nucleic acids of low complexity
WO2004048528A3 (en) Sampling method and apparatus for amplification reaction analysis
WO2005038427A3 (en) Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses
WO2005042764A3 (en) Primers, methods and kits for amplifying or detecting human leukocyte antigen alleles
WO2007035836A3 (en) Accelerated class i and class ii hla dna sequence-based typing
DE602006010288D1 (de) Nachweis biologischer DNA
EP1942196A3 (de) Late-Pcr
ATE446386T1 (de) Verfahren zur überwachung genomischer dna von organismen

Legal Events

Date Code Title Description
8364 No opposition during term of opposition