ATE448302T1 - Nukleinsäureamplifikation auf basis von kügelchenemulsion - Google Patents

Nukleinsäureamplifikation auf basis von kügelchenemulsion

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Publication number
ATE448302T1
ATE448302T1 AT04706051T AT04706051T ATE448302T1 AT E448302 T1 ATE448302 T1 AT E448302T1 AT 04706051 T AT04706051 T AT 04706051T AT 04706051 T AT04706051 T AT 04706051T AT E448302 T1 ATE448302 T1 AT E448302T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid amplification
amplification based
dna
bead emulsion
Prior art date
Application number
AT04706051T
Other languages
English (en)
Inventor
Jan Berka
Yi-Ju Chen
John Leamon
Steven Lefkowitz
Kenton Lohman
Vinod Makhijani
Gary Sarkis
Jonathan Rothberg
Michael Weiner
Maithreyan Srinivasan
Original Assignee
454 Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32854649&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE448302(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 454 Corp filed Critical 454 Corp
Application granted granted Critical
Publication of ATE448302T1 publication Critical patent/ATE448302T1/de

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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6867Replicase-based amplification, e.g. using Q-beta replicase
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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    • Y02P20/50Improvements relating to the production of bulk chemicals
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

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  • Bioinformatics & Computational Biology (AREA)
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  • Optics & Photonics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
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AT04706051T 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion ATE448302T1 (de)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US44347103P 2003-01-29 2003-01-29
US46507103P 2003-04-23 2003-04-23
US47631303P 2003-06-06 2003-06-06
US47660203P 2003-06-06 2003-06-06
US47650403P 2003-06-06 2003-06-06
US47659203P 2003-06-06 2003-06-06
US49798503P 2003-08-25 2003-08-25
PCT/US2004/002484 WO2004069849A2 (en) 2003-01-29 2004-01-28 Bead emulsion nucleic acid amplification

Publications (1)

Publication Number Publication Date
ATE448302T1 true ATE448302T1 (de) 2009-11-15

Family

ID=32854649

Family Applications (4)

Application Number Title Priority Date Filing Date
AT04785758T ATE437945T1 (de) 2003-01-29 2004-01-28 Verfahren zur amplifikation und sequenzierung von nukleinsäuren
AT04706051T ATE448302T1 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion
AT09175588T ATE546525T1 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion
AT04706053T ATE461291T1 (de) 2003-01-29 2004-01-28 Zweiendige sequenzierung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT04785758T ATE437945T1 (de) 2003-01-29 2004-01-28 Verfahren zur amplifikation und sequenzierung von nukleinsäuren

Family Applications After (2)

Application Number Title Priority Date Filing Date
AT09175588T ATE546525T1 (de) 2003-01-29 2004-01-28 Nukleinsäureamplifikation auf basis von kügelchenemulsion
AT04706053T ATE461291T1 (de) 2003-01-29 2004-01-28 Zweiendige sequenzierung

Country Status (10)

Country Link
US (15) US7842457B2 (de)
EP (8) EP2145955B1 (de)
JP (9) JP4480715B2 (de)
CN (2) CN101128601B (de)
AT (4) ATE437945T1 (de)
AU (4) AU2004209426B2 (de)
CA (6) CA2513541A1 (de)
DE (4) DE602004024034D1 (de)
ES (5) ES2396245T3 (de)
WO (4) WO2004070007A2 (de)

Families Citing this family (1292)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
GB2342094B (en) 1997-07-07 2002-10-23 Medical Res Council In vitro sorting method
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
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