DK3215616T3 - Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer - Google Patents

Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer Download PDF

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Publication number
DK3215616T3
DK3215616T3 DK15790422.8T DK15790422T DK3215616T3 DK 3215616 T3 DK3215616 T3 DK 3215616T3 DK 15790422 T DK15790422 T DK 15790422T DK 3215616 T3 DK3215616 T3 DK 3215616T3
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Denmark
Prior art keywords
siderophore
chelators
sequencing
sample preparation
dna damage
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Application number
DK15790422.8T
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English (en)
Inventor
Vincent Peter Smith
Rozieres Sohela De
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Illumina Cambridge Ltd
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Publication of DK3215616T3 publication Critical patent/DK3215616T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK15790422.8T 2014-11-05 2015-10-26 Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer DK3215616T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462075708P 2014-11-05 2014-11-05
PCT/US2015/057409 WO2016073237A1 (en) 2014-11-05 2015-10-26 Reducing dna damage during sample preparation and sequencing using siderophore chelators

Publications (1)

Publication Number Publication Date
DK3215616T3 true DK3215616T3 (da) 2020-03-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK15790422.8T DK3215616T3 (da) 2014-11-05 2015-10-26 Reducering af DNA-skade under prøvepræparering og sekventering ved anvendelse af siderophor-chelatorer

Country Status (6)

Country Link
US (2) US9828627B2 (da)
EP (1) EP3215616B1 (da)
CN (1) CN107250358B (da)
DK (1) DK3215616T3 (da)
ES (1) ES2768762T3 (da)
WO (1) WO2016073237A1 (da)

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WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer

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Also Published As

Publication number Publication date
WO2016073237A1 (en) 2016-05-12
CN107250358B (zh) 2021-03-30
CN107250358A (zh) 2017-10-13
US20180051323A1 (en) 2018-02-22
EP3215616B1 (en) 2019-12-18
US10724075B2 (en) 2020-07-28
EP3215616A1 (en) 2017-09-13
US20160122804A1 (en) 2016-05-05
US9828627B2 (en) 2017-11-28
ES2768762T3 (es) 2020-06-23

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