BR112021026562A2 - Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras - Google Patents

Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras

Info

Publication number
BR112021026562A2
BR112021026562A2 BR112021026562A BR112021026562A BR112021026562A2 BR 112021026562 A2 BR112021026562 A2 BR 112021026562A2 BR 112021026562 A BR112021026562 A BR 112021026562A BR 112021026562 A BR112021026562 A BR 112021026562A BR 112021026562 A2 BR112021026562 A2 BR 112021026562A2
Authority
BR
Brazil
Prior art keywords
compositions
capture probe
methods
arrangements
microbead
Prior art date
Application number
BR112021026562A
Other languages
English (en)
Inventor
Segale Darren
E Black Fiona
Dennis Brodin Jeffrey
Berti Lorenzo
Hong Leong Siew
S Fisher Jeffrey
Eckhardt Allen
Zhang Rui
Nah Teo Yin
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of BR112021026562A2 publication Critical patent/BR112021026562A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101—Primer extension
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODOS E COMPOSIÇÕES PARA IDENTIFICAR LIGANTES EM ARRANJOS COM O USO DE ÍNDICES E CÓDIGOS DE BARRAS. Algumas modalidades aqui apresentadas incluem métodos e composições para a detecção de ligantes-alvo em um arranjo. Em algumas modalidades, uma sonda de captura se liga especificamente a um ligante-alvo a partir de uma amostra, o local de uma microesfera que compreende a sonda de captura em um arranjo é determinado, e a microesfera é decodificada para identificar a sonda de captura e a amostra. Em algumas modalidades, um código de barras é indicativo de uma sonda de captura fixada a uma microesfera; e um índice é indicativo de uma subpopulação de microesferas. Algumas modalidades se referem ao sequenciamento de polinucleotídeos alvo a partir de várias amostras de ácidos nucleicos diferentes em um arranjo de microesferas.
BR112021026562A 2019-09-20 2020-09-18 Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras BR112021026562A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962903108P 2019-09-20 2019-09-20
US201962909014P 2019-10-01 2019-10-01
PCT/US2020/051646 WO2021055864A1 (en) 2019-09-20 2020-09-18 Methods and compositions for identifying ligands on arrays using indexes and barcodes

Publications (1)

Publication Number Publication Date
BR112021026562A2 true BR112021026562A2 (pt) 2022-11-29

Family

ID=72802138

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021026562A BR112021026562A2 (pt) 2019-09-20 2020-09-18 Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras

Country Status (12)

Country Link
US (1) US20210087613A1 (pt)
EP (1) EP4031682A1 (pt)
JP (1) JP2022549048A (pt)
KR (1) KR20220062231A (pt)
CN (1) CN114051535A (pt)
AU (1) AU2020351230A1 (pt)
BR (1) BR112021026562A2 (pt)
CA (1) CA3142535A1 (pt)
IL (1) IL288708A (pt)
MX (1) MX2021015885A (pt)
SG (1) SG11202113372WA (pt)
WO (1) WO2021055864A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2023014950A (es) * 2021-06-24 2024-03-14 Illumina Inc Métodos y composiciones para indexación combinatoria de ácidos nucleicos a base de glóbulos.
CN116536399A (zh) * 2023-07-07 2023-08-04 之江实验室 一种基于微珠测序的基因芯片编解码方法及基因芯片

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5882521A (en) * 1996-04-18 1999-03-16 Waters Investment Ltd. Water-wettable chromatographic media for solid phase extraction
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
EP2159285B1 (en) 2003-01-29 2012-09-26 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050053980A1 (en) 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
SG10201405158QA (en) * 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7323527B1 (en) * 2006-06-08 2008-01-29 Rohm And Haas Company Process for macroporous acrylic resins
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US20150376609A1 (en) * 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
RU2709655C2 (ru) * 2014-10-17 2019-12-19 Иллумина Кембридж Лимитед Транспозиция с сохранением сцепления генов
WO2017075294A1 (en) * 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017123647A1 (en) * 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background
JP7280181B2 (ja) * 2016-10-01 2023-05-23 バークレー ライツ，インコーポレイテッド Ｄｎａバーコード組成物及びマイクロ流体デバイスによるインサイチュ同定法
AU2017345562B2 (en) 2016-10-19 2024-01-25 Illumina Singapore Pte. Ltd. Methods for chemical ligation of nucleic acids
SG11201909697TA (en) 2017-05-01 2019-11-28 Illumina Inc Optimal index sequences for multiplex massively parallel sequencing
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
EP3870704A4 (en) 2018-10-25 2023-01-11 Illumina, Inc. METHODS AND COMPOSITIONS FOR IDENTIFYING LIGANDS ON NETWORKS USING INDICES AND BARCODES

Also Published As

Publication number Publication date
WO2021055864A1 (en) 2021-03-25
CA3142535A1 (en) 2021-03-25
MX2021015885A (es) 2022-03-22
EP4031682A1 (en) 2022-07-27
KR20220062231A (ko) 2022-05-16
AU2020351230A1 (en) 2022-01-20
SG11202113372WA (en) 2021-12-30
US20210087613A1 (en) 2021-03-25
JP2022549048A (ja) 2022-11-24
CN114051535A (zh) 2022-02-15
IL288708A (en) 2022-02-01

Similar Documents

Publication Publication Date Title
BR112021026562A2 (pt) Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras
BR112022019754A2 (pt) Ensaios para detecção de sars-cov-2
NZ630277A (en) Methods and compositions for diagnosis and prognosis of renal injury and renal failure
BR112012021202A2 (pt) aparelho e metodos para preparacao, reacao e deteccao integradas de amostras
EA201370063A1 (ru) Фосфолипидом рака
EA201070349A1 (ru) Определение вариаций количества копий, способы и системы
BR112012011230A2 (pt) fatores de risco e previsão de infarto do miocárdio
EP4083233A3 (en) Method for using gene expression to determine prognosis of prostate cancer
DK1889059T3 (da) Fremgangsmåde til forbedret immunoassay
BRPI0614151A2 (pt) métodos para determinar quais os membros, individualmente ou em combinação, de um conjunto elementar de testes intermediários são preditivos de um conjunto elementar de resultados de teste de uso final, para prever se uma amostra candidato atenderá ao conjunto elementar de resultados de teste de uso final, para prever se uma amostra de lubrificante candidato será aprovada em um teste de uso final escolhido para lubrificantes, para determinar qual a combinação de membros de um conjunto elementar de testes intermediários que tem uma correlação elevada com um conjunto elementar de resultados de teste de uso final e para escolher componentes e quantidades de componentes em lubrificantes, fluidos funcionais ou graxas
BR112012019542A2 (pt) "método para avaliar o estado renal em um indivíduo, medição de um ou mais biomarcadores, e, kit"
BRPI0610092B8 (pt) métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado
ATE411525T1 (de) Nachweis einer histonmodifikation in zellfreien nukleosomen
BR112022019764A2 (pt) Ensaios para detecção de sars-cov-2
MX2021003768A (es) Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.
RU2011140471A (ru) Способ диагностики эндометриоза и диагностический набор для эндометриоза
BR112022011676A2 (pt) Kit para detectar um analito de interesse em uma pluralidade de amostras líquidas biológicas, método para detectá-lo e /ou quantificá-lo e cartucho para realizar tal método
RU2012148711A (ru) Способ и набор для диагностики злокачественной опухоли
EP2808397A3 (en) Method for obtaining information on colon cancer and marker and kit for obtaining information on colon cancer
RU2019104870A (ru) Комбинированный тест на наличие колоректального рака
CN102395977B (zh) 核酸定量的方法
BRPI0607016A2 (pt) marcadores polipeptìdicos para o diagnóstico de cáncer de próstata
Roth et al. Rapid Biosensing Method for Detecting Protein–DNA Interactions
DE602005013430D1 (de) Verwendung von asc als marker für kolorektalkarzinome
SG11201811433TA (en) Mmp1 gene transcript for use as marker for diagnosis of ovarian cancer prognosis, and test method