BR112012021202A2 - aparelho e metodos para preparacao, reacao e deteccao integradas de amostras - Google Patents

aparelho e metodos para preparacao, reacao e deteccao integradas de amostras

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Publication number
BR112012021202A2
BR112012021202A2 BR112012021202A BR112012021202A BR112012021202A2 BR 112012021202 A2 BR112012021202 A2 BR 112012021202A2 BR 112012021202 A BR112012021202 A BR 112012021202A BR 112012021202 A BR112012021202 A BR 112012021202A BR 112012021202 A2 BR112012021202 A2 BR 112012021202A2
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BR
Brazil
Prior art keywords
sample
reaction
detection
methods
nucleic acid
Prior art date
Application number
BR112012021202A
Other languages
English (en)
Other versions
BR112012021202B1 (pt
Inventor
Bruce A Johnson
Bruce Richardson
Dylan Hilmer Bird
Jesus Ching
Keith E Moravick
Original Assignee
Genturadx Usa Inc
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Publication date
Application filed by Genturadx Usa Inc filed Critical Genturadx Usa Inc
Publication of BR112012021202A2 publication Critical patent/BR112012021202A2/pt
Publication of BR112012021202B1 publication Critical patent/BR112012021202B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/24Apparatus for enzymology or microbiology tube or bottle type
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    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
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    • B01F31/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01F35/712Feed mechanisms for feeding fluids
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    • B01F35/717Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/7174Feed mechanisms characterised by the means for feeding the components to the mixer using pistons, plungers or syringes
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    • CCHEMISTRY; METALLURGY
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    • C12M1/38Temperature-responsive control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • GPHYSICS
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    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01F2101/23Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01F35/00Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
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    • B01F35/715Feeding the components in several steps, e.g. successive steps
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
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    • B01L2200/025Align devices or objects to ensure defined positions relative to each other
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10T436/255Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

aparelho e métodos para preparação, reação e detecção integradas de amostras. trata-se de cartuchos para o isolamento de uma amostra biológica e ensaios biológicos a jusante na amostra. em uma modalidade, uma amostra de ácido nucléico é isolada de uma amostra biológica e a amostra de ácido nucléico é amplificada, por exemplo, através da reação em cadeia da polimerase. os cartuchos aqui proprocionados também podem ser usados para isolar amostras de não-ácido nucléico, por exemplo, proteínas, ensaios de ligação. proporcionam-se, também, instrumentos para realiza ensaios biológicos a jusante e detectar os resultados dos ensaios.
BR112012021202A 2010-02-23 2011-02-23 aparelho e métodos para preparação, reação e detecção integradas de amostras BR112012021202B1 (pt)

Applications Claiming Priority (2)

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