MX2021003768A - Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra. - Google Patents

Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.

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Publication number
MX2021003768A
MX2021003768A MX2021003768A MX2021003768A MX2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A MX 2021003768 A MX2021003768 A MX 2021003768A
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MX
Mexico
Prior art keywords
compositions
barcodes
capture probe
indexes
arrays
Prior art date
Application number
MX2021003768A
Other languages
English (en)
Inventor
Lorenzo Berti
Darren Segale
Fiona E Black
Jeffrey Dennis Brodin
Jeffrey Fisher
Siew Hong Leong
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Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021003768A publication Critical patent/MX2021003768A/es

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    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/101—Primer extension
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Algunas modalidades proporcionadas en la presente descripción incluyen métodos y composiciones para la detección de ligandos objetivo en un arreglo. En algunas modalidades, una sonda de captura se une específicamente a un ligando objetivo de una muestra, se determina la ubicación de un glóbulo que comprende la sonda de captura en un arreglo, y el glóbulo se decodifica para identificar la sonda de captura y la muestra. En algunas modalidades, un código de barras es indicativo de una sonda de captura unida a un glóbulo; y un índice es indicativo de una subpoblación de glóbulos. En algunas modalidades, el código de barras y el índice se determinan por secuenciación.
MX2021003768A 2018-10-25 2019-10-23 Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra. MX2021003768A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862750370P 2018-10-25 2018-10-25
US201962903108P 2019-09-20 2019-09-20
PCT/US2019/057704 WO2020086746A1 (en) 2018-10-25 2019-10-23 Methods and compositions for identifying ligands on arrays using indexes and barcodes

Publications (1)

Publication Number Publication Date
MX2021003768A true MX2021003768A (es) 2021-05-27

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MX2021003768A MX2021003768A (es) 2018-10-25 2019-10-23 Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.

Country Status (12)

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US (2) US11667957B2 (es)
EP (1) EP3870704A4 (es)
JP (1) JP2022503873A (es)
KR (1) KR20210084441A (es)
CN (2) CN112840023B (es)
AU (1) AU2019364418A1 (es)
BR (1) BR112021006190A2 (es)
CA (1) CA3113270A1 (es)
IL (1) IL281551A (es)
MX (1) MX2021003768A (es)
SG (1) SG11202102722SA (es)
WO (1) WO2020086746A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112021026562A2 (pt) 2019-09-20 2022-11-29 Illumina Inc Métodos e composições para identificar ligantes em arranjos com o uso de índices e códigos de barras
WO2023096675A1 (en) * 2021-11-23 2023-06-01 Pleno, Inc. Encoded endonuclease assays
CN113416766B (zh) * 2021-07-02 2022-08-19 苏州拉索生物芯片科技有限公司 一种生物芯片的解码方法
CN116536399A (zh) * 2023-07-07 2023-08-04 之江实验室 一种基于微珠测序的基因芯片编解码方法及基因芯片

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
JP3662850B2 (ja) * 1998-06-24 2005-06-22 イルミナ インコーポレイテッド 微小球を有するアレイセンサーのデコード
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
AU2004205311A1 (en) * 1999-04-20 2004-09-23 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
WO2003002979A2 (en) * 2001-06-28 2003-01-09 Illumina, Inc. Multiplex decoding of array sensors with microspheres
CA2513889A1 (en) 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
DE102008061774A1 (de) * 2008-12-11 2010-06-17 Febit Holding Gmbh Indexierung von Nukleinsäure-Populationen
EP2401398B1 (en) * 2009-02-27 2017-02-08 Koninklijke Philips N.V. Genomic selection and sequencing using encoded microcarriers
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
JP6633514B2 (ja) * 2013-04-25 2020-01-22 ファイアフライ バイオワークス， インコーポレイテッド 標的核酸の多重化分析
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015172080A1 (en) 2014-05-08 2015-11-12 Fluidigm Corporation Integrated single cell sequencing
US10829814B2 (en) * 2014-06-19 2020-11-10 Illumina, Inc. Methods and compositions for single cell genomics
JP6838969B2 (ja) * 2014-06-26 2021-03-03 １０エックス ジェノミクス， インコーポレイテッド 個々の細胞または細胞集団由来の核酸の分析方法
CA2964799A1 (en) * 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition
CN107532207B (zh) * 2015-04-10 2021-05-07 空间转录公司 生物样本的空间区别、多重核酸分析
KR102490693B1 (ko) * 2016-05-16 2023-01-19 나노스트링 테크놀로지스, 인크. 샘플 중 표적 핵산을 검출하는 방법
AU2017278350B2 (en) * 2016-06-06 2023-10-19 Redvault Biosciences, Lp Target reporter constructs and uses thereof
WO2018064640A1 (en) * 2016-10-01 2018-04-05 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
WO2018174827A1 (en) * 2017-03-24 2018-09-27 National University Of Singapore Methods for multiplex detection of molecules
WO2018186947A1 (en) * 2017-04-06 2018-10-11 MyOmicsDx, Inc Method and kit for targeted enrichment of nucleic acids

Also Published As

Publication number Publication date
KR20210084441A (ko) 2021-07-07
IL281551A (en) 2021-05-31
US11667957B2 (en) 2023-06-06
AU2019364418A1 (en) 2021-04-22
WO2020086746A1 (en) 2020-04-30
EP3870704A4 (en) 2023-01-11
SG11202102722SA (en) 2021-04-29
CN112840023B (zh) 2024-03-01
BR112021006190A2 (pt) 2021-06-29
CN112840023A (zh) 2021-05-25
US20230313275A1 (en) 2023-10-05
CN117778535A (zh) 2024-03-29
US20200131570A1 (en) 2020-04-30
JP2022503873A (ja) 2022-01-12
EP3870704A1 (en) 2021-09-01
CA3113270A1 (en) 2020-04-30

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