LT2768607T - Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti - Google Patents
Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atliktiInfo
- Publication number
- LT2768607T LT2768607T LTEPPCT/US2012/057363T LT12057363T LT2768607T LT 2768607 T LT2768607 T LT 2768607T LT 12057363 T LT12057363 T LT 12057363T LT 2768607 T LT2768607 T LT 2768607T
- Authority
- LT
- Lithuania
- Prior art keywords
- nucleic acid
- high efficiency
- small volume
- acid synthesis
- multiwell plate
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00457—Dispensing or evacuation of the solid phase support
- B01J2219/00459—Beads
- B01J2219/00466—Beads in a slurry
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/005—Beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
- B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00655—Making arrays on substantially continuous surfaces the compounds being bound to magnets embedded in or on the solid supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00709—Type of synthesis
- B01J2219/00713—Electrochemical synthesis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161539303P | 2011-09-26 | 2011-09-26 | |
PCT/US2012/057363 WO2013049227A2 (en) | 2011-09-26 | 2012-09-26 | High efficiency, small volume nucleic acid synthesis |
Publications (1)
Publication Number | Publication Date |
---|---|
LT2768607T true LT2768607T (lt) | 2021-12-27 |
Family
ID=47073517
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LTEPPCT/US2012/057363T LT2768607T (lt) | 2011-09-26 | 2012-09-26 | Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti |
Country Status (5)
Country | Link |
---|---|
US (5) | US20130109596A1 (lt) |
EP (2) | EP2768607B1 (lt) |
CN (2) | CN103945931B (lt) |
LT (1) | LT2768607T (lt) |
WO (1) | WO2013049227A2 (lt) |
Families Citing this family (62)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10741034B2 (en) | 2006-05-19 | 2020-08-11 | Apdn (B.V.I.) Inc. | Security system and method of marking an inventory item and/or person in the vicinity |
WO2013049227A2 (en) | 2011-09-26 | 2013-04-04 | Geneart Ag | High efficiency, small volume nucleic acid synthesis |
EP3597764A3 (en) | 2012-02-01 | 2020-05-06 | SGI-DNA, Inc. | Material and methods for the synthesis of error-minimized nucleic acid molecules |
CA2872017A1 (en) * | 2012-05-09 | 2013-11-14 | Apdn (B.V.I.) Inc. | Verification of physical encryption taggants using digital representatives and authentications thereof |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) * | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2900543C (en) | 2013-02-08 | 2023-01-31 | 10X Genomics, Inc. | Partitioning and processing of analytes and other species |
US9963740B2 (en) | 2013-03-07 | 2018-05-08 | APDN (B.V.I.), Inc. | Method and device for marking articles |
US10563240B2 (en) | 2013-03-14 | 2020-02-18 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
KR102351838B1 (ko) | 2013-08-05 | 2022-01-18 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
CA2926436A1 (en) | 2013-10-07 | 2015-04-16 | Judith Murrah | Multimode image and spectral reader |
WO2015065924A2 (en) * | 2013-10-28 | 2015-05-07 | Massachusetts Institute Of Technology | Hydrogel microstructures with immiscible fluid isolation for small reaction volumes |
WO2015063767A1 (en) * | 2013-10-31 | 2015-05-07 | Yeda Research And Development Co. Ltd. | Gene synthesis and cell-free cloning using programmable microfluidics |
WO2015142990A1 (en) | 2014-03-18 | 2015-09-24 | Apdn (B.V.I.) Inc. | Encryped optical markers for security applications |
US10745825B2 (en) | 2014-03-18 | 2020-08-18 | Apdn (B.V.I.) Inc. | Encrypted optical markers for security applications |
EP3161160B1 (en) | 2014-06-26 | 2021-10-13 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
US11033593B2 (en) | 2014-11-14 | 2021-06-15 | Basf Plant Science Company Gmbh | Brassica events LBFLFK and LBFDAU and methods for detection thereof |
CA3147259A1 (en) * | 2014-12-09 | 2016-06-16 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
WO2016126987A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Compositions and methods for synthetic gene assembly |
WO2016126882A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
JP2018505688A (ja) | 2015-02-24 | 2018-03-01 | 10エックス ゲノミクス,インコーポレイテッド | 標的化核酸配列包括度(coverage)のための方法 |
EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
CA2998169A1 (en) | 2015-09-18 | 2017-03-23 | Twist Bioscience Corporation | Oligonucleic acid variant libraries and synthesis thereof |
WO2017053450A1 (en) | 2015-09-22 | 2017-03-30 | Twist Bioscience Corporation | Flexible substrates for nucleic acid synthesis |
CN106799196A (zh) * | 2015-11-26 | 2017-06-06 | 上海美迪维康生物科技有限公司 | Dna原位合成半导体芯片及其控制方法 |
CA3006867A1 (en) | 2015-12-01 | 2017-06-08 | Twist Bioscience Corporation | Functionalized surfaces and preparation thereof |
EP3442719B1 (en) | 2016-04-11 | 2021-09-01 | APDN (B.V.I.) Inc. | Method of marking cellulosic products |
SG11201901563UA (en) | 2016-08-22 | 2019-03-28 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
WO2018057526A2 (en) | 2016-09-21 | 2018-03-29 | Twist Bioscience Corporation | Nucleic acid based data storage |
US10995371B2 (en) | 2016-10-13 | 2021-05-04 | Apdn (B.V.I.) Inc. | Composition and method of DNA marking elastomeric material |
CA3047128A1 (en) | 2016-12-16 | 2018-06-21 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
EP4310183A3 (en) | 2017-01-30 | 2024-02-21 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
US10920274B2 (en) | 2017-02-21 | 2021-02-16 | Apdn (B.V.I.) Inc. | Nucleic acid coated submicron particles for authentication |
CN110892485B (zh) | 2017-02-22 | 2024-03-22 | 特韦斯特生物科学公司 | 基于核酸的数据存储 |
CN110913865A (zh) | 2017-03-15 | 2020-03-24 | 特韦斯特生物科学公司 | 免疫突触的变体文库及其合成 |
WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
US10696965B2 (en) | 2017-06-12 | 2020-06-30 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
CN111566125A (zh) | 2017-09-11 | 2020-08-21 | 特韦斯特生物科学公司 | Gpcr结合蛋白及其合成 |
CN111565834B (zh) | 2017-10-20 | 2022-08-26 | 特韦斯特生物科学公司 | 用于多核苷酸合成的加热的纳米孔 |
SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
AU2019205269A1 (en) | 2018-01-04 | 2020-07-30 | Twist Bioscience Corporation | DNA-based digital information storage |
SG11202011467RA (en) | 2018-05-18 | 2020-12-30 | Twist Bioscience Corp | Polynucleotides, reagents, and methods for nucleic acid hybridization |
EP3814494B1 (en) | 2018-06-29 | 2023-11-01 | Thermo Fisher Scientific GENEART GmbH | High throughput assembly of nucleic acid molecules |
WO2020010029A1 (en) * | 2018-07-02 | 2020-01-09 | Quan Qing | Apparatus and methods for synthesizing biopolymers |
SG11202109283UA (en) | 2019-02-26 | 2021-09-29 | Twist Bioscience Corp | Variant nucleic acid libraries for antibody optimization |
EP3930753A4 (en) | 2019-02-26 | 2023-03-29 | Twist Bioscience Corporation | NUCLEIC ACID VARIANT BANKS FOR THE GLP1 RECEPTOR |
GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
CN111835507B (zh) * | 2019-04-19 | 2023-04-25 | 富联国基(上海)电子有限公司 | 基于基因芯片的非对称加密解密方法 |
US11332738B2 (en) | 2019-06-21 | 2022-05-17 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
WO2021178809A1 (en) | 2020-03-06 | 2021-09-10 | Life Technologies Corporation | High sequence fidelity nucleic acid synthesis and assembly |
EP4298246A1 (en) | 2021-02-26 | 2024-01-03 | Avery Digital Data, Inc. | Semiconductor chip devices and methods for polynucleotide synthesis |
CN113096731B (zh) * | 2021-03-12 | 2022-01-11 | 云舟生物科技(广州)有限公司 | 载体生产周期的预估方法、计算机存储介质及电子设备 |
WO2023116712A1 (zh) * | 2021-12-20 | 2023-06-29 | 英诺维尔智能科技(苏州)有限公司 | 一种高通量核酸合成芯片及其使用方法 |
CN114457067A (zh) * | 2022-02-10 | 2022-05-10 | 中国科学院天津工业生物技术研究所 | 一种低成本快速去除dna合成中错误的方法 |
Family Cites Families (104)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4652639A (en) | 1982-05-06 | 1987-03-24 | Amgen | Manufacture and expression of structural genes |
US5512439A (en) | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
ATE173023T1 (de) | 1991-09-06 | 1998-11-15 | Res Dev Foundation | Dna-sequenzen kodierend für das polypeptid gelonin |
US5869644A (en) | 1992-04-15 | 1999-02-09 | The Johns Hopkins University | Synthesis of diverse and useful collections of oligonucleotidies |
GB9208921D0 (en) | 1992-04-24 | 1992-06-10 | Isis Innovation | Electrochemical treatment of surfaces |
US5472672A (en) | 1993-10-22 | 1995-12-05 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for polymer synthesis using arrays |
US6331274B1 (en) * | 1993-11-01 | 2001-12-18 | Nanogen, Inc. | Advanced active circuits and devices for molecular biological analysis and diagnostics |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5580759A (en) | 1994-02-03 | 1996-12-03 | Board Of Regents, The University Of Texas System | Construction of recombinant DNA by exonuclease recession |
US5514789A (en) | 1994-04-21 | 1996-05-07 | Barrskogen, Inc. | Recovery of oligonucleotides by gas phase cleavage |
US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
US5786464C1 (en) | 1994-09-19 | 2012-04-24 | Gen Hospital Corp | Overexpression of mammalian and viral proteins |
ES2181900T3 (es) | 1995-06-07 | 2003-03-01 | Invitrogen Corp | Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica. |
US6964861B1 (en) | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US6426183B1 (en) | 1995-12-21 | 2002-07-30 | Kenneth L. Beattie | Oligonucleotide microarrays: direct covalent attachment to glass |
US5851804A (en) | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
ES2243393T3 (es) | 1996-06-04 | 2005-12-01 | University Of Utah Research Foundation | Monitorizacion de la hibridacion durante la pcr. |
US6495318B2 (en) | 1996-06-17 | 2002-12-17 | Vectorobjects, Llc | Method and kits for preparing multicomponent nucleic acid constructs |
ZA975891B (en) * | 1996-07-05 | 1998-07-23 | Combimatrix Corp | Electrochemical solid phase synthesis of polymers |
US6114148C1 (en) | 1996-09-20 | 2012-05-01 | Gen Hospital Corp | High level expression of proteins |
US6110668A (en) | 1996-10-07 | 2000-08-29 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Gene synthesis method |
US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
DE19736591A1 (de) | 1997-08-22 | 1999-02-25 | Peter Prof Dr Hegemann | Verfahren zum Herstellen von Nukleinsäurepolymeren |
EP1538206B1 (en) | 1997-09-16 | 2010-03-24 | Centocor, Inc. | Method for the complete chemical synthesis and assembly of genes and genomes |
US7351578B2 (en) | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
EP1291420B1 (en) | 1997-12-05 | 2009-11-25 | Europäisches Laboratorium Für Molekularbiologie (Embl) | Novel DNA cloning method relying on the E.coli RECE/RECT recombination system |
US6093302A (en) | 1998-01-05 | 2000-07-25 | Combimatrix Corporation | Electrochemical solid phase synthesis |
US6083726A (en) | 1998-02-03 | 2000-07-04 | Lucent Technologies, Inc. | Methods for polynucleotide synthesis and articles for polynucleotide hybridization |
DE60030957T2 (de) | 1999-02-16 | 2007-06-14 | Applera Corp., Foster City | Kugelabgabesystem |
DE60042969D1 (de) | 1999-03-02 | 2009-10-29 | Life Technologies Corp | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren |
US6296702B1 (en) | 1999-03-15 | 2001-10-02 | Pe Corporation (Ny) | Apparatus and method for spotting a substrate |
US6335438B1 (en) | 1999-03-22 | 2002-01-01 | Geir Fonnum | Method for the manufacture of amino group containing support matrices, support matrices prepared by the method, and use of the support matrices |
CA2369293C (en) | 1999-04-09 | 2010-06-08 | Dynal Particles As | Process for the preparation of monodisperse polymer particles |
US8137906B2 (en) | 1999-06-07 | 2012-03-20 | Sloning Biotechnology Gmbh | Method for the synthesis of DNA fragments |
US20070059752A1 (en) | 1999-06-09 | 2007-03-15 | Biosearch Technologies, Inc. | Fluorescence energy transfer probes with stabilized conformations |
US6355412B1 (en) | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
CN1367823A (zh) | 1999-08-02 | 2002-09-04 | 阿彻·丹尼尔斯-米德兰公司 | 氨基酸生产的代谢工程 |
US7604996B1 (en) | 1999-08-18 | 2009-10-20 | Illumina, Inc. | Compositions and methods for preparing oligonucleotide solutions |
US6391576B1 (en) | 1999-10-13 | 2002-05-21 | Japan Bioindustry Association | Method for isolating a microbe |
US20060128006A1 (en) | 1999-11-10 | 2006-06-15 | Gerhardt Antimony L | Hydrodynamic capture and release mechanisms for particle manipulation |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
WO2002038728A2 (en) | 2000-11-09 | 2002-05-16 | Invitrogen Corporation | Method for removing a universal linker from an oligonucleotide |
US20020146732A1 (en) | 2001-02-02 | 2002-10-10 | Padgett Hal S. | Method of increasing complementarity in a heteroduplex |
GB0121155D0 (en) | 2001-08-31 | 2001-10-24 | Isis Innovation | Treatment of substrates |
WO2003029494A1 (en) | 2001-10-03 | 2003-04-10 | Micrologix Biotech Inc. | Attachment of thiophosphate tethered oligonucleotides to a solid surface |
DE50213541D1 (de) | 2002-01-11 | 2009-06-25 | Biospring Ges Fuer Biotechnolo | Verfahren zur Herstellung von DNA |
US7078211B2 (en) | 2002-02-01 | 2006-07-18 | Large Scale Biology Corporation | Nucleic acid molecules encoding endonucleases and methods of use thereof |
US20040219516A1 (en) | 2002-07-18 | 2004-11-04 | Invitrogen Corporation | Viral vectors containing recombination sites |
DE10260805A1 (de) | 2002-12-23 | 2004-07-22 | Geneart Gmbh | Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins |
US7202264B2 (en) | 2003-01-31 | 2007-04-10 | Isis Pharmaceuticals, Inc. | Supports for oligomer synthesis |
US9177387B2 (en) | 2003-02-11 | 2015-11-03 | Sony Computer Entertainment Inc. | Method and apparatus for real time motion capture |
US20060127920A1 (en) | 2004-02-27 | 2006-06-15 | President And Fellows Of Harvard College | Polynucleotide synthesis |
WO2005095590A2 (en) | 2004-03-29 | 2005-10-13 | Monsanto Technology Llc | Sperm suspensions for sorting into x or y chromosome-bearing enriched populations |
DK1776460T3 (da) | 2004-08-03 | 2010-04-12 | Geneart Ag | Fremgangsmåde til modulering af genekspression ved ændring af CpG-indholdet |
US7820412B2 (en) | 2004-08-27 | 2010-10-26 | Wisconsin Alumni Research Foundation | Method of error reduction in nucleic acid populations |
JP4890457B2 (ja) | 2004-09-02 | 2012-03-07 | アイシス ファーマシューティカルズ, インコーポレーテッド | オリゴマー合成のための高分子ビーズ |
US20060063159A1 (en) | 2004-09-22 | 2006-03-23 | Applera Corporation | Post-synthesis processing system for supported oligonucleotides, and method |
US20070034513A1 (en) | 2005-03-25 | 2007-02-15 | Combimatrix Corporation | Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array |
US8507197B2 (en) | 2005-08-10 | 2013-08-13 | Ge Healthcare Bio-Sciences Corp. | Quality control methods for arrayed oligonucleotides |
US20080187969A1 (en) | 2005-10-27 | 2008-08-07 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
CA2642514C (en) | 2005-12-02 | 2011-06-07 | Lei Young | Synthesis of error-minimized nucleic acid molecules |
US7829505B2 (en) | 2005-12-27 | 2010-11-09 | Council Of Scientific And Industrial Research | Method for construction of oligonucleotide microarrays |
WO2007087377A2 (en) * | 2006-01-25 | 2007-08-02 | Massachusetts Institute Of Technology | Photoelectrochemical synthesis of high density combinatorial polymer arrays |
US20070231805A1 (en) | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
WO2010006166A2 (en) | 2008-07-09 | 2010-01-14 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US8637317B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Method of washing beads |
WO2009140671A2 (en) | 2008-05-16 | 2009-11-19 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods for manipulating beads |
US20070292954A1 (en) | 2006-04-21 | 2007-12-20 | The Brigham And Women's Hospital, Inc. | Generation of recombinant DNA by sequence-and ligation-independent cloning |
DE102006039479A1 (de) | 2006-08-23 | 2008-03-06 | Febit Biotech Gmbh | Programmierbare Oligonukleotidsynthese |
US8328635B2 (en) | 2006-09-13 | 2012-12-11 | Igt | System and method for rewarding players based on personal interests or attributes |
EP2115144A1 (en) | 2007-02-05 | 2009-11-11 | Philipps-Universität Marburg | Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases |
US8028591B2 (en) | 2007-05-11 | 2011-10-04 | Applied Biosystems Llc | System and method for selective retrieval of support beads |
JP2009002808A (ja) * | 2007-06-21 | 2009-01-08 | Hitachi Ltd | Dna計測システム及びdna計測方法 |
AU2008276308A1 (en) * | 2007-07-13 | 2009-01-22 | The Board Of Trustees Of The Leland Stanford Junior University | Method and apparatus using electric field for improved biological assays |
EP2190988A4 (en) | 2007-08-07 | 2010-12-22 | Agency Science Tech & Res | INTEGRATED MICROFLUID DEVICE FOR GENE SYNTHESIS |
JP5618413B2 (ja) | 2007-10-08 | 2014-11-05 | シンセティック ゲノミクス、インク. | 大型核酸のアッセンブリー |
DE102007057802B3 (de) | 2007-11-30 | 2009-06-10 | Geneart Ag | Steganographische Einbettung von Informationen in kodierenden Genen |
US8815576B2 (en) | 2007-12-27 | 2014-08-26 | Lawrence Livermore National Security, Llc. | Chip-based sequencing nucleic acids |
SG188101A1 (en) | 2008-02-15 | 2013-03-28 | Synthetic Genomics Inc | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules |
GB0803109D0 (en) | 2008-02-20 | 2008-03-26 | Gene Bridges Gmbh | Method of nucleic acid recombination |
AU2009253665B2 (en) | 2008-05-26 | 2014-10-09 | Ferag Ag | Device and method for inserting flat objects into a folded printed product |
WO2010030776A1 (en) | 2008-09-10 | 2010-03-18 | Genscript Corporation | Homologous recombination-based dna cloning methods and compositions |
DK2175018T3 (da) | 2008-10-08 | 2011-08-22 | Icon Genetics Gmbh | Fremgangsmåde til ren kloning |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US20100301398A1 (en) * | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
CN101397587B (zh) * | 2008-11-06 | 2011-01-12 | 上海交通大学 | 基于基因置换技术的活菌内标的制备方法 |
US20100216648A1 (en) | 2009-02-20 | 2010-08-26 | Febit Holding Gmbh | Synthesis of sequence-verified nucleic acids |
JP2011000079A (ja) | 2009-06-19 | 2011-01-06 | Univ Of Tokyo | 粒子を操作する方法及びマイクロ流体装置 |
US8397374B2 (en) | 2009-07-16 | 2013-03-19 | National Taipei University Of Technology | Micro spherical stylus manufacturing machine |
US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
CN102648294A (zh) * | 2009-12-07 | 2012-08-22 | 普罗格诺西斯生物科学公司 | 肽展示阵列 |
WO2011102802A1 (en) | 2010-02-18 | 2011-08-25 | Agency For Science, Technology And Research | Method for reducing mismatches in double-stranded dna molecules |
EP2542676A1 (en) | 2010-03-05 | 2013-01-09 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
CN201901669U (zh) * | 2010-07-23 | 2011-07-20 | 浙江大学 | 用于数字核酸扩增的集成流路芯片装置 |
DE102010038431A1 (de) | 2010-07-26 | 2012-01-26 | Diasys Diagnostic Systems Gmbh | Messkassette und Messvorrichtung für die Detektion von Zielmolekülen in einer flüssigen Probe durch Messung von Fluoreszenzemission nach Anregung im evaneszenten Feld |
US20120258870A1 (en) | 2010-11-22 | 2012-10-11 | The University Of Chicago | Methods, Systems, and/or Use of Oligonucleotide Conjugates to Develop Panels for Use in Assays and Detections |
WO2013049227A2 (en) | 2011-09-26 | 2013-04-04 | Geneart Ag | High efficiency, small volume nucleic acid synthesis |
US9438358B2 (en) | 2012-09-14 | 2016-09-06 | Freescale Semiconductor, Inc. | Receiver unit and method for correcting a value of a receive signal strength indicator |
WO2014065758A1 (en) | 2012-10-25 | 2014-05-01 | Star Array Pte Ltd | A method of isolating nucleic acids in an aqueous sample using microfluidic device |
US10563240B2 (en) | 2013-03-14 | 2020-02-18 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
CA2967135A1 (en) | 2014-11-19 | 2016-05-26 | Imec Vzw | Microbubble generator device, systems and method to fabricate |
CA3147259A1 (en) | 2014-12-09 | 2016-06-16 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
WO2017100283A1 (en) | 2015-12-09 | 2017-06-15 | Life Technologies Corporation | Detection and quantification of nucleic acid molecules associated with a surface |
-
2012
- 2012-09-26 WO PCT/US2012/057363 patent/WO2013049227A2/en active Application Filing
- 2012-09-26 CN CN201280057089.4A patent/CN103945931B/zh active Active
- 2012-09-26 US US13/627,819 patent/US20130109596A1/en not_active Abandoned
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- 2012-09-26 CN CN201710180114.2A patent/CN107058059B/zh active Active
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CN103945931B (zh) | 2017-03-22 |
WO2013049227A2 (en) | 2013-04-04 |
US20200172898A1 (en) | 2020-06-04 |
US11046953B2 (en) | 2021-06-29 |
US20130109596A1 (en) | 2013-05-02 |
EP2768607A2 (en) | 2014-08-27 |
US10519439B2 (en) | 2019-12-31 |
US20150344876A1 (en) | 2015-12-03 |
WO2013049227A3 (en) | 2014-01-09 |
US20170260521A1 (en) | 2017-09-14 |
EP3964285A1 (en) | 2022-03-09 |
CN103945931A (zh) | 2014-07-23 |
CN107058059B (zh) | 2020-08-07 |
US20210388346A1 (en) | 2021-12-16 |
EP2768607B1 (en) | 2021-08-18 |
CN107058059A (zh) | 2017-08-18 |
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