DK3553180T3 - Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf - Google Patents
Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf Download PDFInfo
- Publication number
- DK3553180T3 DK3553180T3 DK16923153.7T DK16923153T DK3553180T3 DK 3553180 T3 DK3553180 T3 DK 3553180T3 DK 16923153 T DK16923153 T DK 16923153T DK 3553180 T3 DK3553180 T3 DK 3553180T3
- Authority
- DK
- Denmark
- Prior art keywords
- procedure
- single cell
- library construction
- sequence library
- cell sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2016/108940 WO2018103025A1 (zh) | 2016-12-07 | 2016-12-07 | 单细胞测序文库的构建方法及其应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3553180T3 true DK3553180T3 (da) | 2022-07-25 |
Family
ID=62490570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16923153.7T DK3553180T3 (da) | 2016-12-07 | 2016-12-07 | Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11434483B2 (da) |
| EP (1) | EP3553180B1 (da) |
| CN (1) | CN109996892B (da) |
| DK (1) | DK3553180T3 (da) |
| ES (1) | ES2922281T3 (da) |
| WO (1) | WO2018103025A1 (da) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9388465B2 (en) | 2013-02-08 | 2016-07-12 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN113249435A (zh) | 2014-06-26 | 2021-08-13 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| AU2016207023B2 (en) | 2015-01-12 | 2019-12-05 | 10X Genomics, Inc. | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
| CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN109526228B (zh) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| EP3710594B1 (en) * | 2017-11-16 | 2023-07-19 | The Regents of The University of Colorado, A Body Corporate | Methods to measure functional heterogeneity among single cells |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN112005115A (zh) | 2018-02-12 | 2020-11-27 | 10X基因组学有限公司 | 表征来自单个细胞或细胞群体的多种分析物的方法 |
| EP3775271A1 (en) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| WO2020009665A1 (en) * | 2018-07-06 | 2020-01-09 | Agency For Science, Technology And Research | Method for single-cell transcriptome and accessible regions sequencing |
| CN109023537B (zh) * | 2018-09-04 | 2021-10-08 | 上海交通大学 | 一种微量dna样品高通量测序文库的构建技术 |
| US11739321B2 (en) | 2019-01-07 | 2023-08-29 | Agilent Technologies, Inc. | Compositions and methods for genomic DNA and gene expression analysis in single cells |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| SG11202108788TA (en) | 2019-02-12 | 2021-09-29 | 10X Genomics Inc | Methods for processing nucleic acid molecules |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020232635A1 (zh) * | 2019-05-21 | 2020-11-26 | 深圳华大智造科技有限公司 | 基于甲基化dna目标区域构建测序文库及系统和应用 |
| CN110952147B (zh) * | 2019-12-18 | 2023-05-05 | 南方科技大学 | 单细胞基因组测序用的dna文库的构建方法 |
| WO2021226917A1 (en) * | 2020-05-14 | 2021-11-18 | Singleron (Nanjing) Biotechnologies, Ltd. | Novel method of one-step whole transcriptome amplification |
| CN111575348B (zh) * | 2020-05-19 | 2024-01-09 | 广州微远医疗器械有限公司 | 宏基因组文库及建库方法和应用 |
| CN112011596A (zh) * | 2020-09-11 | 2020-12-01 | 深圳华大因源医药科技有限公司 | 一种同时检测多种微生物基因组的方法、溶液和试剂盒 |
| CN112143784A (zh) * | 2020-09-29 | 2020-12-29 | 生物岛实验室 | 空间组学测序、单细胞表观转录组学测序及定位标识方法 |
| CN112280828A (zh) * | 2020-10-22 | 2021-01-29 | 上海交通大学医学院 | 一种降低单细胞扩增偏倚性的离体组织细胞核分离方法 |
| CN112941636A (zh) * | 2021-04-02 | 2021-06-11 | 武汉博越致和生物科技有限公司 | 一种适用于简化转录组测序的文库构建方法及配套试剂盒 |
| CN113462748A (zh) * | 2021-05-11 | 2021-10-01 | 温氏食品集团股份有限公司 | Dna测序文库的制备方法及试剂盒 |
| CN113249439A (zh) * | 2021-05-11 | 2021-08-13 | 杭州圣庭医疗科技有限公司 | 一种简化dna甲基化文库及转录组共测序文库的构建方法 |
| CN113337590A (zh) * | 2021-06-03 | 2021-09-03 | 深圳华大基因股份有限公司 | 一种二代测序方法和文库构建方法 |
| CN114645074A (zh) * | 2022-03-07 | 2022-06-21 | 华南农业大学 | 一种单花蕾微量rna建库方法 |
| CN115612720A (zh) * | 2022-09-28 | 2023-01-17 | 上海欧易生物医学科技有限公司 | 一种适用于猕猴桃根组织单细胞测序的植物细胞核制备以及悬液优化方法 |
| CN115948621A (zh) * | 2023-01-18 | 2023-04-11 | 珠海舒桐医疗科技有限公司 | 一种基于月经血dna的hpv筛查方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS53122047A (en) | 1977-03-30 | 1978-10-25 | Sanden Corp | Electromagnetic clutch |
| JPS5924538U (ja) | 1982-08-09 | 1984-02-15 | サンデン株式会社 | 電磁クラツチ |
| JP4174896B2 (ja) | 1998-09-22 | 2008-11-05 | 株式会社デンソー | 電磁クラッチ |
| KR20040034191A (ko) | 2002-10-21 | 2004-04-28 | 한라공조주식회사 | 전자클러치용 디스크 및 허브 조립체 |
| US7044284B2 (en) | 2003-06-10 | 2006-05-16 | Halla Climate Control Corporation | Disc and hub assembly for electromagnetic clutch in a compressor |
| EP4321628A2 (en) * | 2013-05-23 | 2024-02-14 | The Board of Trustees of the Leland Stanford Junior University | Transposition into native chromatin for personal epigenomics |
| CN104561244B (zh) * | 2013-10-15 | 2017-03-22 | 天津大学 | 基于二代测序技术的微生物单细胞转录组分析方法 |
| CN113005108A (zh) * | 2014-04-15 | 2021-06-22 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN105463089A (zh) * | 2015-12-21 | 2016-04-06 | 同济大学 | 应用于斑马鱼胚胎的易接近转座酶核染色质高通量测序实验的方法 |
-
2016
- 2016-12-07 DK DK16923153.7T patent/DK3553180T3/da active
- 2016-12-07 CN CN201680091050.2A patent/CN109996892B/zh active Active
- 2016-12-07 EP EP16923153.7A patent/EP3553180B1/en active Active
- 2016-12-07 WO PCT/CN2016/108940 patent/WO2018103025A1/zh unknown
- 2016-12-07 US US16/466,741 patent/US11434483B2/en active Active
- 2016-12-07 ES ES16923153T patent/ES2922281T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US11434483B2 (en) | 2022-09-06 |
| EP3553180A1 (en) | 2019-10-16 |
| CN109996892B (zh) | 2023-08-29 |
| US20190338279A1 (en) | 2019-11-07 |
| EP3553180B1 (en) | 2022-05-04 |
| WO2018103025A1 (zh) | 2018-06-14 |
| CN109996892A (zh) | 2019-07-09 |
| EP3553180A4 (en) | 2020-08-12 |
| ES2922281T3 (es) | 2022-09-12 |
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