CN104561244B - 基于二代测序技术的微生物单细胞转录组分析方法 - Google Patents
基于二代测序技术的微生物单细胞转录组分析方法 Download PDFInfo
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- CN104561244B CN104561244B CN201310481921.XA CN201310481921A CN104561244B CN 104561244 B CN104561244 B CN 104561244B CN 201310481921 A CN201310481921 A CN 201310481921A CN 104561244 B CN104561244 B CN 104561244B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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CN201310481921.XA CN104561244B (zh) | 2013-10-15 | 2013-10-15 | 基于二代测序技术的微生物单细胞转录组分析方法 |
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CN201310481921.XA CN104561244B (zh) | 2013-10-15 | 2013-10-15 | 基于二代测序技术的微生物单细胞转录组分析方法 |
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CN104561244A CN104561244A (zh) | 2015-04-29 |
CN104561244B true CN104561244B (zh) | 2017-03-22 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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ES2922281T3 (es) * | 2016-12-07 | 2022-09-12 | Mgi Tech Co Ltd | Método para construir una biblioteca de secuenciación de una célula individual y uso del mismo |
CN106701995B (zh) * | 2017-02-20 | 2019-11-26 | 元码基因科技(北京)股份有限公司 | 通过单细胞转录组测序进行细胞质量控制的方法 |
CN108193283A (zh) * | 2017-12-21 | 2018-06-22 | 上海美吉生物医药科技有限公司 | 一种单细胞转录组文库建立方法及其应用 |
CN109971849A (zh) * | 2017-12-28 | 2019-07-05 | 安诺优达基因科技(北京)有限公司 | 肝癌相关基因的单细胞多样本混合建库方法 |
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- 2013-10-15 CN CN201310481921.XA patent/CN104561244B/zh active Active
Non-Patent Citations (3)
Title |
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mRNA-Seq whole-transcriptome analysis of a single cell;Fuchou Tang等;《NATURE METHODS》;20090419;第6卷(第5期);第1页右栏第2段,第9页左栏最后1段至第10页左栏第2段 * |
RT-qPCR based quantitative analysis of gene expression in single bacterial cells;Weimin Gao等;《Journal of Microbiological Methods》;20110401;第85卷;摘要 * |
Single cell transcriptomics of neighboring hyphae of Aspergillus niger;Charissa de Bekker等;《Genome Biology》;20111231;第12卷;第224页右栏第1段 * |
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Inventor after: Wang Jiangxin Inventor after: Chen Lei Inventor after: Zhang Weiwen Inventor before: Wang Jiangxin Inventor before: Chen Lei Inventor before: Zhang Weiwen Inventor before: Xiao Huazhi Inventor before: Fang Wenzhi Inventor before: Li Yan |
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Address after: 300350 District, Jinnan District, Tianjin Haihe Education Park, 135 beautiful road, Beiyang campus of Tianjin University Patentee after: Tianjin University Address before: 300072 Tianjin City, Nankai District Wei Jin Road No. 92 Patentee before: Tianjin University |
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