JP2002503336A - 微量流通システムおよびその使用方法 - Google Patents
微量流通システムおよびその使用方法Info
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- JP2002503336A JP2002503336A JP54973398A JP54973398A JP2002503336A JP 2002503336 A JP2002503336 A JP 2002503336A JP 54973398 A JP54973398 A JP 54973398A JP 54973398 A JP54973398 A JP 54973398A JP 2002503336 A JP2002503336 A JP 2002503336A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B81—MICROSTRUCTURAL TECHNOLOGY
- B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
- B81B1/00—Devices without movable or flexible elements, e.g. microcapillary devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
- Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.微量流通デバイスであって、 検出領域および流出口を備えた主流通経路と; 前記検出領域においてまたは前記検出領域よりも上流側において、90°より も小さな上流側開き角度でもって前記主流通経路に対して合流する、少なくとも 2つの流入経路と; を具備することを特徴とする微量流通デバイス。 2.請求項1記載の微量流通デバイスにおいて、 2つの流入経路を具備していることを特徴とする微量流通デバイス。 3.請求項1記載の微量流通デバイスにおいて、 3つの流入経路を具備していることを特徴とする微量流通デバイス。 4.請求項3記載の微量流通デバイスにおいて、 前記主流通経路は、該主流通経路に対しての前記各流入経路の各合流箇所のと ころにまたはそこよりも下流側に、少なくとも1つの検出領域を備えていること を特徴とする微量流通デバイス。 5.請求項1記載の微量流通デバイスにおいて、 前記主流通経路は、およそ深さ0.1μm×幅0.1μm〜およそ深さ1mm ×幅2mmとされていることを特徴とする微量流通デバイス。 6.請求項1記載の微量流通デバイスにおいて、 第1流入経路は、およそ深さ0.1μm×幅0.1μm〜およそ深さ1mm× 幅2mmとされていることを特徴とする微量流通デバイス。 7.請求項1記載の微量流通デバイスにおいて、 さらに、流通誘起力を印加するための手段を具備していることを特徴とする微量 流通デバイス。 8.請求項6記載の微量流通デバイスにおいて、 前記流通誘起力が、電気力であることを特徴とする微量流通デバイス。 9.請求項6記載の微量流通デバイスにおいて、 前記流通誘起力が、負の流体圧力または正の流体圧力であることを特徴とする 微量流通デバイス。 10.請求項9記載の微量流通デバイスにおいて、 負の流体圧力または正の流体圧力が、前記流出口に印加されることを特徴とす る微量流通デバイス。 11.請求項1記載の微量流通デバイスにおいて、 少なくとも1つの前記流入経路および前記主流通経路内に、細胞を含有してい ることを特徴とする微量流通デバイス。 12.請求項1記載の微量流通デバイスにおいて、 前記主流通経路内に、白血球、カルシウム色素、および、候補化合物を含有し ていることを特徴とする微量流通デバイス。 13.請求項1に記載された微量流通デバイスを複数具備してなる観測デバイス であって、 前記複数の微量流通デバイスのそれぞれの検出領域を共有していることを特徴 とする観測デバイス。 14.請求項13記載の観測デバイスにおいて、 前記複数の微量流通デバイスのそれぞれの前記主流通経路どうしが、前記共有 検出領域において、実質的に互いに平行とされていることを特徴とする観測デバ イス。 15.請求項1に記載された微量流通デバイスを複数具備してなる観測デバイス であって、 前記複数の微量流通デバイスのそれぞれの前記主流通経路どうしが、それぞれ の検出領域において、実質的に互いに平行とされていることを特徴とする観測デ バイス。 16.細胞に対しての候補化合物の効果を微量流通デバイス内において観測する ための方法であって、 (a)検出領域および流出口を備えた主流通経路と、前記検出領域においてま たは前記検出領域よりも上流側において前記主流通経路に対して合流する少なく とも2つの流入経路と、を備えた微量流通デバイスを準備し; (b)少なくとも1つの細胞を、第1流入経路に導入するとともに、前記候補 化合物を、第2流入経路に導入し; (c)前記流出口に向けての、前記細胞の流れおよび前記候補化合物の流れを 誘起し; (d)前記第2流入経路と前記主流通経路との合流箇所において、前記細胞と 前記候補化合物とを混合し; (e)前記細胞に対しての前記候補化合物の効果を、前記検出領域において観 測する; ことを特徴とする方法。 17.請求項16記載の方法において、 前記微量流通デバイスが、3つの流入経路を備え、 第2候補化合物が、第3流入経路に導入されることを特徴とする方法。 18.請求項16記載の方法において、 前記細胞が前記検出領域内に位置している時に、前記流れを停止させることを 特徴とする方法。 19.請求項17記載の方法において、 前記主流通経路が複数の検出領域を備えているとともに、各検出領域が、前記 主流通経路に対しての前記各流入経路の各合流箇所のところにまたはそこよりも 下流側に位置している場合に、 複数の検出領域の各々において観測を行うことを特徴とする方法。 20.請求項16記載の方法において、 研究されるべき前記候補化合物が、細胞活性化剤であるとともに、前記細胞が 、リンパ球であることを特徴とする方法。 21.請求項17記載の方法において、 細胞が第1流入経路に導入され、細胞活性化剤が第2流入経路に導入され、候 補化合物が第3流入経路に導入されることを特徴とする方法。 22.請求項21記載の方法において、 研究されるべき前記候補化合物が抑制剤であり、前記細胞がリンパ球であるこ とを特徴とする方法。 23.請求項16記載の方法において、 前記主流通経路および前記各流入経路が、タンパク質、糖タンパク質、リン脂 質、親水性ポリマー、および、疎水性ポリマーからなるグループの中から選択さ れた物質でコーティングされていることを特徴とする方法。 24.請求項23記載の方法において、 前記主流通経路および前記各流入経路が、タンパク質でコーティングされてい ることを特徴とする方法。 25.請求項23記載の方法において、 前記流れを、電気力によって誘起することを特徴とする方法。 26.請求項24記載の方法において、 前記流れを、正の流体圧力または負の流体圧力によって誘起することを特徴と する方法。 27.リンパ球におけるカルシウム流を研究するための方法であって、 (a)検出領域を備えた主流通経路と、前記検出領域よりも上流側において前 記主流通経路に対して順次的に合流する少なくとも2つの流入経路と、前記検出 領域よりも下流側に配置された流出口と、を備えた徴量流通デバイスを準備し; (b)リンパ球を第1流入経路に導入するとともに、活性化剤を第2流入経路 に導入し; (c)前記流出口に向けての、前記リンパ球の流れおよび前記活性化剤の流れ を誘起し; (d)前記第2流入経路と前記主流通経路との合流箇所において、前記リンパ 球と前記活性化剤とを混合し; (e)前記リンパ球に対しての前記活性化剤の効果を観測する; ことを特徴とする方法。 28.請求項27記載の方法において、 前記微量流通デバイスが、3つの流入経路を備え、 候補化合物を第3流入経路に導入し、 前記検出領域において、前記リンパ球に対しての前記候補化合物の効果を観測 することを特徴とする方法。 29.白血球のローリングを研究するための方法であって、 (a)検出領域および流出口を備えた主流通経路であるとともに、前記検出領 域における壁には細胞付着性分子が取り付けられている主流通経路と、前記検出 領域のところにおいてまたは前記検出領域よりも上流側において前記主流通経路 に対して順次的に合流する少なくとも2つの流入経路と、を備えた微量流通デバ イスを準備し; (b)少なくとも1つの白血球を、第1流入経路に導入し; (c)少なくとも1つの白血球と候補化合物とを、第2流入経路に導入し; (d)前記主流通経路内において前記白血球の流れおよび前記候補化合物の流 れを誘起し; (e)前記白血球と前記細胞付着性分子とを相互作用させ; (f)前記候補化合物の存在下においてまたはそれがない状態において、前記 検出領域において前記白血球のローリングを観測する; ことを特徴とする方法。 30.請求項29記載の方法において、 前記微量流通デバイスが、約30μm〜約500μmという断面積とされた主 流通経路を備えていることを特徴とする方法。 31.請求項29記載の方法において、 前記微量流通デバイスが、さらに、第3流入経路を備え、 緩衝剤を、この第3流入経路に導入することを特徴とする方法。 32.請求項1記載のデバイスにおいて、 前記主流通経路の断面積が、場所によって変化していることを特徴とするデバ イス。 33.請求項32記載のデバイスにおいて、 前記断面積変化が、細胞拘束領域を形成していることを特徴とするデバイス。 34.請求項32記載のデバイスにおいて、 前記断面積変化が、堰によってもたらされていることを特徴とするデバイス。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/858,087 | 1997-05-16 | ||
US08/858,087 US6632619B1 (en) | 1997-05-16 | 1997-05-16 | Microfluidic system and methods of use |
US4702497P | 1997-05-19 | 1997-05-19 | |
US60/047,024 | 1997-05-19 | ||
PCT/CA1998/000481 WO1998052691A1 (en) | 1997-05-16 | 1998-05-15 | Microfluidic system and methods of use |
Publications (1)
Publication Number | Publication Date |
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JP2002503336A true JP2002503336A (ja) | 2002-01-29 |
Family
ID=26724544
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP54973398A Ceased JP2002503336A (ja) | 1997-05-16 | 1998-05-15 | 微量流通システムおよびその使用方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US6900021B1 (ja) |
EP (1) | EP0981408B1 (ja) |
JP (1) | JP2002503336A (ja) |
AT (1) | ATE264717T1 (ja) |
AU (1) | AU734957B2 (ja) |
CA (1) | CA2286601A1 (ja) |
DE (1) | DE69823347T2 (ja) |
WO (1) | WO1998052691A1 (ja) |
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JP2004340758A (ja) * | 2003-05-15 | 2004-12-02 | Toshiba Mach Co Ltd | 微細流路およびこれを含むマイクロ化学チップ |
JP2005505754A (ja) * | 2001-07-25 | 2005-02-24 | ザ トラスティーズ オブ プリンストン ユニバーシテイ | 高スループットのマクロ分子分析用のナノチャンネル・アレイ並びにその準備および使用 |
JP2005077397A (ja) * | 2003-08-29 | 2005-03-24 | National Institute For Materials Science | 流れ変動構造及びマイクロミキサ |
JP2005233802A (ja) * | 2004-02-20 | 2005-09-02 | Yokogawa Electric Corp | 物理量計測装置およびその装置を用いて行う物理量校正方法 |
JP2005533636A (ja) * | 2002-04-16 | 2005-11-10 | プリンストン ユニバーシティ | マイクロ流体とナノ流体間のインターフェース用勾配構造と、その製造方法および使用方法 |
JP2006503581A (ja) * | 2002-10-22 | 2006-02-02 | サーフェイス ロジックス,インコーポレイティド | 白血球遊走をモニタリングするための装置及び方法 |
JP2006078475A (ja) * | 2004-08-09 | 2006-03-23 | Nsk Ltd | 反応器及びその製造方法 |
JP2006087336A (ja) * | 2004-09-22 | 2006-04-06 | Shimadzu Corp | 細胞分析装置 |
JP2006125900A (ja) * | 2004-10-27 | 2006-05-18 | Hitachi High-Technologies Corp | 液体搬送基板、分析システム、分析方法 |
JPWO2004090090A1 (ja) * | 2003-04-09 | 2006-07-06 | 株式会社 エフェクター細胞研究所 | 細胞走化性検出装置 |
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Families Citing this family (211)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6168948B1 (en) | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
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US6149882A (en) * | 1998-06-09 | 2000-11-21 | Symyx Technologies, Inc. | Parallel fixed bed reactor and fluid contacting apparatus |
US6416642B1 (en) * | 1999-01-21 | 2002-07-09 | Caliper Technologies Corp. | Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection |
US20030235920A1 (en) * | 2000-02-28 | 2003-12-25 | James Wyatt | Diagnostic device and method |
DE19927534B4 (de) * | 1999-06-16 | 2008-01-31 | Merck Patent Gmbh | Vorrichtung zur Probenaufgabe |
JP4387624B2 (ja) * | 1999-06-16 | 2009-12-16 | イーペー フェアヴェルトゥングス ゲーエムベーハー | 試料作成装置 |
FR2798867A1 (fr) * | 1999-09-23 | 2001-03-30 | Commissariat Energie Atomique | Dispositif de distribution de fluides dans un microsysteme fluidique |
US6432290B1 (en) | 1999-11-26 | 2002-08-13 | The Governors Of The University Of Alberta | Apparatus and method for trapping bead based reagents within microfluidic analysis systems |
CA2290731A1 (en) * | 1999-11-26 | 2001-05-26 | D. Jed Harrison | Apparatus and method for trapping bead based reagents within microfluidic analysis system |
US6589729B2 (en) * | 2000-02-04 | 2003-07-08 | Caliper Technologies Corp. | Methods, devices, and systems for monitoring time dependent reactions |
JP3442338B2 (ja) | 2000-03-17 | 2003-09-02 | 株式会社日立製作所 | Dna分析装置、dna塩基配列決定装置、dna塩基配列決定方法、および反応モジュール |
DE10017791A1 (de) * | 2000-04-10 | 2001-10-11 | Basf Ag | Verfahren und Vorrichtung zur Mikrodosierung kleinster Flüssigkeitsmengen für Biopolymerarrays |
JP4606543B2 (ja) * | 2000-04-13 | 2011-01-05 | パナソニック株式会社 | 光学特性計測装置における被検溶液量確認方法および計測系制御方法 |
US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
GB2366793B (en) * | 2000-09-13 | 2005-03-09 | Imperial College | Chemical processing system and method |
WO2002023163A1 (en) | 2000-09-15 | 2002-03-21 | California Institute Of Technology | Microfabricated crossflow devices and methods |
US7326563B2 (en) | 2000-11-08 | 2008-02-05 | Surface Logix, Inc. | Device and method for monitoring leukocyte migration |
DE60227649D1 (de) * | 2001-04-03 | 2008-08-28 | Micronics Inc | Getrennt fokussierendes zytometer |
EP1384022A4 (en) | 2001-04-06 | 2004-08-04 | California Inst Of Techn | AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES |
US9678038B2 (en) | 2001-07-25 | 2017-06-13 | The Trustees Of Princeton University | Nanochannel arrays and their preparation and use for high throughput macromolecular analysis |
US8440093B1 (en) | 2001-10-26 | 2013-05-14 | Fuidigm Corporation | Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels |
WO2003048736A2 (en) * | 2001-12-05 | 2003-06-12 | University Of Washington | Microfluidic device and surface decoration process for solid phase affinity binding assays |
CA2480728A1 (en) | 2002-04-01 | 2003-10-16 | Fluidigm Corporation | Microfluidic particle-analysis systems |
US6808075B2 (en) | 2002-04-17 | 2004-10-26 | Cytonome, Inc. | Method and apparatus for sorting particles |
US6976590B2 (en) | 2002-06-24 | 2005-12-20 | Cytonome, Inc. | Method and apparatus for sorting particles |
US9943847B2 (en) | 2002-04-17 | 2018-04-17 | Cytonome/St, Llc | Microfluidic system including a bubble valve for regulating fluid flow through a microchannel |
EP1502108A4 (en) * | 2002-04-24 | 2007-03-21 | Surface Logix Inc | DEVICE AND METHOD FOR MONITORING THE LEUKOCYTE WALKING |
US7901939B2 (en) | 2002-05-09 | 2011-03-08 | University Of Chicago | Method for performing crystallization and reactions in pressure-driven fluid plugs |
US7129091B2 (en) * | 2002-05-09 | 2006-10-31 | University Of Chicago | Device and method for pressure-driven plug transport and reaction |
JP4106977B2 (ja) | 2002-06-21 | 2008-06-25 | 株式会社日立製作所 | 分析チップ及び分析装置 |
AU2003270722A1 (en) * | 2002-09-16 | 2004-04-30 | Cytonome/St, Llc | Method and apparatus for sorting particles |
EP1551753A2 (en) | 2002-09-25 | 2005-07-13 | California Institute Of Technology | Microfluidic large scale integration |
JP5695287B2 (ja) | 2002-10-02 | 2015-04-01 | カリフォルニア インスティテュート オブ テクノロジー | 微小流体の核酸解析 |
JP2006512092A (ja) | 2002-12-30 | 2006-04-13 | ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア | 病原体の検出および分析のための方法および装置 |
US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
JP2004305076A (ja) * | 2003-04-04 | 2004-11-04 | Canon Inc | 対象物に対する修飾装置 |
EP1636332A4 (en) * | 2003-05-06 | 2007-08-29 | Bellbrook Labs Llc | THREE-DIMENSIONAL CELL CULTURES IN A SMALL-SCALE FLUID HANDLING SYSTEM |
CA2468014C (en) * | 2003-06-04 | 2016-03-22 | Inverness Medical Switzerland Gmbh | Flow sensing for determination of assay results |
WO2004113491A1 (ja) * | 2003-06-20 | 2004-12-29 | Nitto Denko Corporation | 細胞マイクロチップ |
JP2005027659A (ja) * | 2003-06-20 | 2005-02-03 | Nitto Denko Corp | 細胞マイクロチップ |
US7413712B2 (en) | 2003-08-11 | 2008-08-19 | California Institute Of Technology | Microfluidic rotary flow reactor matrix |
WO2005040348A2 (en) | 2003-10-23 | 2005-05-06 | Georgetown University | Method for two- and three-dimensional microassembly of patterns and structures |
DE102004005193B4 (de) * | 2004-02-02 | 2006-08-24 | Medion Diagnostics Gmbh | Vorrichtung zur Separation einzelner Partikel von Partikel-Agglutinationen |
US8058056B2 (en) | 2004-03-12 | 2011-11-15 | The Regents Of The University Of California | Method and apparatus for integrated cell handling and measurements |
US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
US20050227350A1 (en) * | 2004-04-13 | 2005-10-13 | Agency For Science, Technology And Research | Device and method for studying cell migration and deformation |
US7799553B2 (en) | 2004-06-01 | 2010-09-21 | The Regents Of The University Of California | Microfabricated integrated DNA analysis system |
US9477233B2 (en) | 2004-07-02 | 2016-10-25 | The University Of Chicago | Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets |
CA2600899C (en) * | 2004-07-16 | 2014-04-01 | Simon Fraser University | Microfluidic device and method of using same |
US7745207B2 (en) | 2006-02-03 | 2010-06-29 | IntegenX, Inc. | Microfluidic devices |
EP2261650A3 (en) | 2004-09-15 | 2011-07-06 | IntegenX Inc. | Microfluidic devices |
US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
US9260693B2 (en) | 2004-12-03 | 2016-02-16 | Cytonome/St, Llc | Actuation of parallel microfluidic arrays |
US8399205B2 (en) * | 2005-01-21 | 2013-03-19 | The University Of Rochester | Device and method for separation, concentration, and/or purification of cells |
ATE474663T1 (de) * | 2005-06-15 | 2010-08-15 | Rhodia Operations | Mikrofluidikzirkulationsvorrichtung zur bestimmung von parametern einer physikalischen und/oder chemischen transformation und verwendung davon |
US20060285999A1 (en) * | 2005-06-21 | 2006-12-21 | West Virginia University Research Corporation | Apparatus and method for coupling microfluidic systems with electrospray ionization mass spectrometry utilizing a hydrodynamic flow restrictor |
ATE500895T1 (de) * | 2005-07-05 | 2011-03-15 | Ibidi Gmbh | Mikrofluid-vorrichtung und verfahren zur erzeugung diffusiv aufgebauter gradienten |
EP1945793A4 (en) * | 2005-08-11 | 2015-11-25 | Eksigent Technologies Llc | METHODS OF MEASURING BIOCHEMICAL REACTIONS |
US20070107780A1 (en) * | 2005-11-07 | 2007-05-17 | Bristol-Myers Squibb Company | Fluid handling device |
WO2007081386A2 (en) | 2006-01-11 | 2007-07-19 | Raindance Technologies, Inc. | Microfluidic devices and methods of use |
US7749365B2 (en) | 2006-02-01 | 2010-07-06 | IntegenX, Inc. | Optimized sample injection structures in microfluidic separations |
US7766033B2 (en) | 2006-03-22 | 2010-08-03 | The Regents Of The University Of California | Multiplexed latching valves for microfluidic devices and processors |
CN100392316C (zh) | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | 控制液体在微管路中连续流动的流路结构 |
US8293524B2 (en) | 2006-03-31 | 2012-10-23 | Fluxion Biosciences Inc. | Methods and apparatus for the manipulation of particle suspensions and testing thereof |
US9074242B2 (en) | 2010-02-12 | 2015-07-07 | Raindance Technologies, Inc. | Digital analyte analysis |
EP2047910B1 (en) | 2006-05-11 | 2012-01-11 | Raindance Technologies, Inc. | Microfluidic device and method |
US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
DE102006024355B4 (de) * | 2006-05-19 | 2008-04-03 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Mikrofluidische Anordnung zur Detektion von in Proben enthaltenen chemischen, biochemischen Molekülen und/oder Partikeln |
JP4821466B2 (ja) * | 2006-07-03 | 2011-11-24 | 富士ゼロックス株式会社 | 液滴吐出ヘッド |
EP3536396B1 (en) | 2006-08-07 | 2022-03-30 | The President and Fellows of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
US8841116B2 (en) | 2006-10-25 | 2014-09-23 | The Regents Of The University Of California | Inline-injection microdevice and microfabricated integrated DNA analysis system using same |
US20080245740A1 (en) * | 2007-01-29 | 2008-10-09 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Fluidic methods |
US20110039303A1 (en) | 2007-02-05 | 2011-02-17 | Stevan Bogdan Jovanovich | Microfluidic and nanofluidic devices, systems, and applications |
WO2008097559A2 (en) | 2007-02-06 | 2008-08-14 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
AU2008232616B2 (en) | 2007-03-28 | 2014-08-07 | Bionano Genomics, Inc. | Methods of macromolecular analysis using nanochannel arrays |
US20090034359A1 (en) * | 2007-04-04 | 2009-02-05 | The Regents Of The University Of California | Stopped flow, quenched flow and continuous flow reaction method and apparatus |
WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
WO2009015296A1 (en) | 2007-07-24 | 2009-01-29 | The Regents Of The University Of California | Microfabricated dropley generator |
US20100227767A1 (en) | 2007-07-26 | 2010-09-09 | Boedicker James Q | Stochastic confinement to detect, manipulate, and utilize molecules and organisms |
US8266791B2 (en) * | 2007-09-19 | 2012-09-18 | The Charles Stark Draper Laboratory, Inc. | Method of fabricating microfluidic structures for biomedical applications |
CN101990516B (zh) | 2008-01-22 | 2015-09-09 | 英特基因有限公司 | 多用试样准备系统及其在集成分析系统中的使用 |
WO2009131722A2 (en) | 2008-01-24 | 2009-10-29 | Sandia National Laboratories | Methods and devices for immobilization of single particles |
US20090234332A1 (en) * | 2008-03-17 | 2009-09-17 | The Charles Stark Draper Laboratory, Inc | Artificial microvascular device and methods for manufacturing and using the same |
WO2009149257A1 (en) | 2008-06-04 | 2009-12-10 | The University Of Chicago | The chemistrode: a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution |
EP2315629B1 (en) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Droplet libraries |
KR101390717B1 (ko) * | 2008-09-02 | 2014-04-30 | 삼성전자주식회사 | 미세유동장치 및 미세유동장치에의 시료주입방법 |
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
US8633015B2 (en) * | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
US9399215B2 (en) | 2012-04-13 | 2016-07-26 | Bio-Rad Laboratories, Inc. | Sample holder with a well having a wicking promoter |
WO2011120024A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet generation for droplet-based assays |
US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
WO2010077322A1 (en) | 2008-12-31 | 2010-07-08 | Microchip Biotechnologies, Inc. | Instrument with microfluidic chip |
US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
AU2010229490B2 (en) | 2009-03-24 | 2015-02-12 | University Of Chicago | Slip chip device and methods |
US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
EP2438154A1 (en) | 2009-06-02 | 2012-04-11 | Integenx Inc. | Fluidic devices with diaphragm valves |
DE112010002222B4 (de) | 2009-06-04 | 2024-01-25 | Leidos Innovations Technology, Inc. (n.d.Ges.d. Staates Delaware) | Mehr-Proben-Mikrofluidchip fur DNA-Analyse |
AU2010256429B2 (en) | 2009-06-05 | 2015-09-17 | Integenx Inc. | Universal sample preparation system and use in an integrated analysis system |
US20110001963A1 (en) * | 2009-07-02 | 2011-01-06 | Durack Gary P | System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system |
US20110003330A1 (en) * | 2009-07-06 | 2011-01-06 | Durack Gary P | Microfluidic device |
US8778279B2 (en) * | 2009-07-06 | 2014-07-15 | Sony Corporation | Microfluidic device |
TW201105971A (en) * | 2009-07-06 | 2011-02-16 | Sony Corp | Microfluidic device having onboard tissue or cell sample handling capability |
CN103335931A (zh) * | 2009-07-07 | 2013-10-02 | 索尼公司 | 微流体装置 |
WO2011005757A1 (en) | 2009-07-07 | 2011-01-13 | Sony Corporation | Microfluidic device adapted for post-centrifugation use with selective sample extraction and methods for its use |
WO2011005754A1 (en) * | 2009-07-08 | 2011-01-13 | Sony Corporation | Microfluidic device having a flow channel within a gain medium |
KR20120125220A (ko) | 2009-07-20 | 2012-11-14 | 실로암 바이오사이언스, 아이엔씨. | 마이크로유체 검정 플랫폼 |
WO2011028539A1 (en) | 2009-09-02 | 2011-03-10 | Quantalife, Inc. | System for mixing fluids by coalescence of multiple emulsions |
US20110082563A1 (en) * | 2009-10-05 | 2011-04-07 | The Charles Stark Draper Laboratory, Inc. | Microscale multiple-fluid-stream bioreactor for cell culture |
WO2011044116A2 (en) * | 2009-10-05 | 2011-04-14 | The Charles Stark Draper Laboratory, Inc. | Three-dimensional microfluidic platforms and methods of use and manufacture thereof |
WO2011042564A1 (en) | 2009-10-09 | 2011-04-14 | Universite De Strasbourg | Labelled silica-based nanomaterial with enhanced properties and uses thereof |
US8940494B2 (en) * | 2009-11-04 | 2015-01-27 | Cfd Research Corporation | Microfluidic assay in idealized microvascular network for characterization of leukocyte adhesion cascade |
US8584703B2 (en) | 2009-12-01 | 2013-11-19 | Integenx Inc. | Device with diaphragm valve |
US10837883B2 (en) | 2009-12-23 | 2020-11-17 | Bio-Rad Laboratories, Inc. | Microfluidic systems and methods for reducing the exchange of molecules between droplets |
US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
US8399198B2 (en) | 2010-03-02 | 2013-03-19 | Bio-Rad Laboratories, Inc. | Assays with droplets transformed into capsules |
EP2556170A4 (en) | 2010-03-25 | 2014-01-01 | Quantalife Inc | TRAPPING TRANSPORT AND DETECTION SYSTEM |
JP2013524169A (ja) | 2010-03-25 | 2013-06-17 | クァンタライフ・インコーポレーテッド | 液滴によるアッセイ用の検出システム |
US8512538B2 (en) | 2010-05-28 | 2013-08-20 | Integenx Inc. | Capillary electrophoresis device |
CN103140755B (zh) | 2010-07-22 | 2016-03-16 | 哈希公司 | 用于碱度分析的芯片上实验室 |
EP2606242A4 (en) | 2010-08-20 | 2016-07-20 | Integenx Inc | MICROFLUIDIC DEVICES HAVING MECHANICALLY SEALED DIAPHRAGM VALVES |
WO2012024658A2 (en) | 2010-08-20 | 2012-02-23 | IntegenX, Inc. | Integrated analysis system |
US9562897B2 (en) | 2010-09-30 | 2017-02-07 | Raindance Technologies, Inc. | Sandwich assays in droplets |
US8961764B2 (en) | 2010-10-15 | 2015-02-24 | Lockheed Martin Corporation | Micro fluidic optic design |
CN103429331B (zh) | 2010-11-01 | 2016-09-28 | 伯乐生命医学产品有限公司 | 用于形成乳液的系统 |
EP3859011A1 (en) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
EP2686449B1 (en) | 2011-03-18 | 2020-11-18 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
EP2702175B1 (en) | 2011-04-25 | 2018-08-08 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
US9556470B2 (en) | 2011-06-02 | 2017-01-31 | Raindance Technologies, Inc. | Enzyme quantification |
US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
CN102242055B (zh) * | 2011-06-03 | 2013-08-14 | 博奥生物有限公司 | 一种精子活力评价及筛选的方法及其专用微流控芯片装置 |
US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
EP2737089B1 (en) | 2011-07-29 | 2017-09-06 | Bio-rad Laboratories, Inc. | Library characterization by digital assay |
US9449296B2 (en) | 2011-08-02 | 2016-09-20 | Kit Check, Inc. | Management of pharmacy kits using multiple acceptance criteria for pharmacy kit segments |
US8990099B2 (en) | 2011-08-02 | 2015-03-24 | Kit Check, Inc. | Management of pharmacy kits |
WO2013025519A1 (en) * | 2011-08-12 | 2013-02-21 | Rutgers, The State University Of New Jersey | High throughput sensitization detection devices and methods |
KR101911435B1 (ko) * | 2011-09-26 | 2018-10-25 | 삼성전자주식회사 | 유체 제어 장치, 이를 포함하는 필터 및 바이오칩 |
US10865440B2 (en) | 2011-10-21 | 2020-12-15 | IntegenX, Inc. | Sample preparation, processing and analysis systems |
US20150136604A1 (en) | 2011-10-21 | 2015-05-21 | Integenx Inc. | Sample preparation, processing and analysis systems |
US9322054B2 (en) | 2012-02-22 | 2016-04-26 | Lockheed Martin Corporation | Microfluidic cartridge |
US9180449B2 (en) | 2012-06-12 | 2015-11-10 | Hach Company | Mobile water analysis |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9388465B2 (en) | 2013-02-08 | 2016-07-12 | 10X Genomics, Inc. | Polynucleotide barcode generation |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
CN113528634A (zh) | 2012-08-14 | 2021-10-22 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9567631B2 (en) | 2012-12-14 | 2017-02-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
USD768872S1 (en) | 2012-12-12 | 2016-10-11 | Hach Company | Cuvette for a water analysis instrument |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
WO2015073999A1 (en) | 2013-11-18 | 2015-05-21 | Integenx Inc. | Cartridges and instruments for sample analysis |
US9171280B2 (en) | 2013-12-08 | 2015-10-27 | Kit Check, Inc. | Medication tracking |
US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
WO2015103367A1 (en) | 2013-12-31 | 2015-07-09 | Raindance Technologies, Inc. | System and method for detection of rna species |
MX2016013156A (es) | 2014-04-10 | 2017-02-14 | 10X Genomics Inc | Dispositivos, sistemas y metodos fluidicos, para encapsular y dividir reactivos, y aplicaciones de los mismos. |
US10208332B2 (en) | 2014-05-21 | 2019-02-19 | Integenx Inc. | Fluidic cartridge with valve mechanism |
CN113249435A (zh) | 2014-06-26 | 2021-08-13 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
US10690627B2 (en) | 2014-10-22 | 2020-06-23 | IntegenX, Inc. | Systems and methods for sample preparation, processing and analysis |
AU2015339148B2 (en) | 2014-10-29 | 2022-03-10 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequencing |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
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EP3936619A1 (en) | 2015-02-24 | 2022-01-12 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
US10167502B2 (en) | 2015-04-03 | 2019-01-01 | Fluxion Biosciences, Inc. | Molecular characterization of single cells and cell populations for non-invasive diagnostics |
US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
EP3882357B1 (en) | 2015-12-04 | 2022-08-10 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
TWI808934B (zh) | 2015-12-31 | 2023-07-21 | 美商伯克利之光生命科技公司 | 經工程化以表現促發炎多肽之腫瘤浸潤細胞 |
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WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US10692316B2 (en) | 2016-10-03 | 2020-06-23 | Gary L. Sharpe | RFID scanning device |
US10482292B2 (en) | 2016-10-03 | 2019-11-19 | Gary L. Sharpe | RFID scanning device |
US10677661B2 (en) * | 2016-11-09 | 2020-06-09 | Northeastern University | Stop-start method in a microfluidic calorimeter |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3562594A4 (en) | 2016-12-30 | 2020-09-09 | The Regents of University of California | PROCESSES FOR SELECTING AND GENERATING T-LYMPHOCYTES MODIFIED BY THE GENOME |
EP4310183A3 (en) | 2017-01-30 | 2024-02-21 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
EP4230746A3 (en) | 2017-05-26 | 2023-11-01 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
US20190088354A1 (en) | 2017-09-01 | 2019-03-21 | Kit Check, Inc. | Identifying discrepancies between events from disparate systems |
SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
SG11202009889VA (en) | 2018-04-06 | 2020-11-27 | 10X Genomics Inc | Systems and methods for quality control in single cell processing |
US11285476B2 (en) * | 2019-01-08 | 2022-03-29 | Interface Fluidics Ltd. | Microfluidic devices and systems, and methods for operating microfluidic devices and systems |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07506430A (ja) * | 1992-05-01 | 1995-07-13 | トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア | 微細加工した検出構造体 |
WO1996004547A1 (en) * | 1994-08-01 | 1996-02-15 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis |
Family Cites Families (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE418017B (sv) | 1978-06-14 | 1981-04-27 | Bifok Ab | Sett att kontinuerligt bestemma olika langsamt reagerande substanser kvantitativt med anvendning av en enda metcell |
IL68507A (en) | 1982-05-10 | 1986-01-31 | Univ Bar Ilan | System and methods for cell selection |
US5310674A (en) | 1982-05-10 | 1994-05-10 | Bar-Ilan University | Apertured cell carrier |
SE455537B (sv) | 1985-01-31 | 1988-07-18 | Bifok Ab | Sett for kemisk analys vid vilken provet och/eller dess reaktionsprodukter bringas att passera en genomstromningscell, samt en apparatur for utovande av settet |
US4676274A (en) * | 1985-02-28 | 1987-06-30 | Brown James F | Capillary flow control |
US5180662A (en) * | 1988-01-05 | 1993-01-19 | The United States Of America As Represented By The Department Of Health And Human Services | Cytotoxic T lymphocyte activation assay |
JP2993982B2 (ja) * | 1988-10-21 | 1999-12-27 | モレキユラー デヴアイシズ コーポレイシヨン | 生細胞に対する細胞作用剤の効果を検出するための方法及び装置 |
US4987084A (en) | 1989-02-21 | 1991-01-22 | Dana Farber Cancer Institute | Method of testing the effect of a molecule on B lymphocyte function |
US5149972A (en) | 1990-01-18 | 1992-09-22 | University Of Massachusetts Medical Center | Two excitation wavelength video imaging microscope |
US5750015A (en) | 1990-02-28 | 1998-05-12 | Soane Biosciences | Method and device for moving molecules by the application of a plurality of electrical fields |
US5770029A (en) * | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
WO1992003734A1 (en) * | 1990-08-20 | 1992-03-05 | Alain De Weck | A method for measuring t-lymphocyte responses by chemiluminescent assays |
US5460945A (en) * | 1991-05-30 | 1995-10-24 | Center For Blood Research, Inc. | Device and method for analysis of blood components and identifying inhibitors and promoters of the inflammatory response |
KR100198868B1 (ko) * | 1991-07-25 | 1999-06-15 | 케네쓰 제이. 울코트 | 세포독성 t 림프구 반응을 유도하는 조성물 |
US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
CA2144174A1 (en) * | 1992-09-14 | 1994-03-31 | Jianmin Bao | Electrophoretically mediated chemical analysis |
JPH06265447A (ja) * | 1993-03-16 | 1994-09-22 | Hitachi Ltd | 微量反応装置およびこれを使用する微量成分測定装置 |
US5776748A (en) | 1993-10-04 | 1998-07-07 | President And Fellows Of Harvard College | Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices and uses therefor |
DE4411268C2 (de) | 1994-03-31 | 2001-02-01 | Danfoss As | Analyseverfahren und Analysevorrichtung |
WO1996014934A1 (en) | 1994-11-14 | 1996-05-23 | Trustees Of The University Of Pennsylvania | Mesoscale sample preparation device and systems for determination and processing of analytes |
CN1145704C (zh) | 1994-11-14 | 2004-04-14 | 宾夕法尼亚州大学信托人 | 中型多核苷酸扩增装置 |
AU5143096A (en) | 1995-03-31 | 1996-10-16 | Novo Nordisk A/S | Method for identifying biologically active substances by the ir effect on living cells |
US5840254A (en) * | 1995-06-02 | 1998-11-24 | Cdc Technologies, Inc. | Apparatus for mixing fluids for analysis |
TW293783B (ja) * | 1995-06-16 | 1996-12-21 | Ciba Geigy Ag | |
US5872010A (en) * | 1995-07-21 | 1999-02-16 | Northeastern University | Microscale fluid handling system |
US5942443A (en) | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
US5726404A (en) * | 1996-05-31 | 1998-03-10 | University Of Washington | Valveless liquid microswitch |
US5804436A (en) | 1996-08-02 | 1998-09-08 | Axiom Biotechnologies, Inc. | Apparatus and method for real-time measurement of cellular response |
US5858648A (en) | 1996-11-04 | 1999-01-12 | Sienna Biotech, Inc. | Assays using reference microparticles |
-
1998
- 1998-05-15 AU AU74218/98A patent/AU734957B2/en not_active Ceased
- 1998-05-15 JP JP54973398A patent/JP2002503336A/ja not_active Ceased
- 1998-05-15 AT AT98921307T patent/ATE264717T1/de not_active IP Right Cessation
- 1998-05-15 CA CA002286601A patent/CA2286601A1/en not_active Abandoned
- 1998-05-15 WO PCT/CA1998/000481 patent/WO1998052691A1/en not_active Application Discontinuation
- 1998-05-15 DE DE69823347T patent/DE69823347T2/de not_active Expired - Fee Related
- 1998-05-15 EP EP98921307A patent/EP0981408B1/en not_active Revoked
- 1998-05-18 US US09/080,546 patent/US6900021B1/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07506430A (ja) * | 1992-05-01 | 1995-07-13 | トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルベニア | 微細加工した検出構造体 |
WO1996004547A1 (en) * | 1994-08-01 | 1996-02-15 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis |
Non-Patent Citations (2)
Title |
---|
ANALYTICAL METHODS AND INSTRUMENTATION. SPECIAL ISSUE MTAS '96. (1996) 113-115, JPNX006032494, ISSN: 0000757586 * |
ANALYTICAL METHODS AND INSTRUMENTATION. SPECIAL ISSUE MTAS '96. (1996) 82-84, JPNX006032495, ISSN: 0000757587 * |
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Also Published As
Publication number | Publication date |
---|---|
AU734957B2 (en) | 2001-06-28 |
DE69823347T2 (de) | 2005-05-12 |
US6900021B1 (en) | 2005-05-31 |
AU7421898A (en) | 1998-12-11 |
DE69823347D1 (de) | 2004-05-27 |
CA2286601A1 (en) | 1998-11-26 |
WO1998052691A1 (en) | 1998-11-26 |
EP0981408A1 (en) | 2000-03-01 |
ATE264717T1 (de) | 2004-05-15 |
EP0981408B1 (en) | 2004-04-21 |
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