DK2954065T3 - Opdeling og bearbejdning af analytter og andre arter - Google Patents

Opdeling og bearbejdning af analytter og andre arter Download PDF

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Publication number
DK2954065T3
DK2954065T3 DK14748569.2T DK14748569T DK2954065T3 DK 2954065 T3 DK2954065 T3 DK 2954065T3 DK 14748569 T DK14748569 T DK 14748569T DK 2954065 T3 DK2954065 T3 DK 2954065T3
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analytes
species
division
processing
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DK14748569.2T
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Benjamin Hindson
Paul Hardenbol
Mirna Jarosz
Michael Schnall-Levin
Serge Saxonov
Kevin Ness
Christopher Hindson
Donald Masquelier
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10X Genomics Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/112Disease subtyping, staging or classification
DK14748569.2T 2013-02-08 2014-02-07 Opdeling og bearbejdning af analytter og andre arter DK2954065T3 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361762435P 2013-02-08 2013-02-08
US201361800223P 2013-03-15 2013-03-15
US201361840403P 2013-06-27 2013-06-27
US201361844804P 2013-07-10 2013-07-10
PCT/US2014/015424 WO2014124336A2 (en) 2013-02-08 2014-02-07 Partitioning and processing of analytes and other species

Publications (1)

Publication Number Publication Date
DK2954065T3 true DK2954065T3 (da) 2021-09-06

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US (8) US9388465B2 (da)
EP (4) EP2954065B1 (da)
JP (4) JP2016511243A (da)
KR (3) KR102190198B1 (da)
CN (2) CN105102697A (da)
AU (1) AU2014214682B2 (da)
BR (1) BR112015019159A2 (da)
CA (2) CA2900481A1 (da)
DK (1) DK2954065T3 (da)
WO (2) WO2014124338A1 (da)

Families Citing this family (230)

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Publication number Priority date Publication date Assignee Title
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CN101946010B (zh) 2007-12-21 2014-08-20 哈佛大学 用于核酸测序的系统和方法
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
WO2010104597A2 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Scale-up of microfluidic devices
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
CA2778816C (en) 2009-10-27 2018-07-31 President And Fellows Of Harvard College Droplet creation techniques
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US20140057799A1 (en) 2010-12-16 2014-02-27 Gigagen System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012249759A1 (en) 2011-04-25 2013-11-07 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
JP6122843B2 (ja) 2011-05-23 2017-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重エマルジョンを含むエマルジョンの制御
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
AU2013226081B2 (en) 2012-02-27 2018-06-14 Becton, Dickinson And Company Compositions and kits for molecular counting
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
KR102402446B1 (ko) 2013-08-28 2022-05-30 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
WO2015200891A1 (en) 2014-06-26 2015-12-30 10X Technologies, Inc. Processes and systems for nucleic acid sequence assembly
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN113249435A (zh) 2014-06-26 2021-08-13 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
CA2997906A1 (en) 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
ES2925014T3 (es) * 2014-09-12 2022-10-13 Univ Leland Stanford Junior Identificación y uso de ácidos nucleicos circulantes
WO2016061517A2 (en) * 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US10060913B2 (en) 2016-09-19 2018-08-28 Massachusetts Institute Of Technology Systems including janus droplets capable of binding an analyte and changing orientation to provide a detectable change
US10005058B2 (en) * 2014-10-31 2018-06-26 Massachusetts Institute Of Technology Compositions and methods for arranging colloid phases
US11119098B2 (en) 2014-10-31 2021-09-14 Massachusetts Institute Of Technology Systems including Janus droplets
JP6914192B2 (ja) 2014-10-31 2021-08-04 マサチューセッツ インスティテュート オブ テクノロジー 乳剤形成のための組成および方法
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN107209814B (zh) 2015-01-13 2021-10-15 10X基因组学有限公司 用于使结构变异和相位信息可视化的系统和方法
MX2017010142A (es) 2015-02-09 2017-12-11 10X Genomics Inc Sistemas y metodos para determinar variacion estructural y ajuste de fases con datos de recuperacion de variantes.
KR20170135834A (ko) * 2015-02-10 2017-12-08 일루미나, 인코포레이티드 세포 성분을 분석하기 위한 방법 및 조성물
US20180010176A1 (en) * 2015-02-13 2018-01-11 Abhijit Ajit Patel Methods for highly parallel and accurate measurement of nucleic acids
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016145416A2 (en) * 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2016145409A1 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Genotype and phenotype coupling
WO2016149096A1 (en) 2015-03-13 2016-09-22 President And Fellows Of Harvard College Determination of cells using amplification
CN113846144B (zh) 2015-03-17 2023-09-26 生物辐射实验室股份有限公司 检测基因组编辑
US20180073073A1 (en) * 2015-03-18 2018-03-15 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
JP6828007B2 (ja) 2015-04-10 2021-02-10 スペーシャル トランスクリプトミクス アクチボラグ 生物学的試料の空間識別されるマルチプレックスな核酸分析
WO2016168351A1 (en) * 2015-04-15 2016-10-20 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
JP2018511341A (ja) 2015-04-17 2018-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子配列決定および他の適用のためのバーコード化システムおよび方法
CN114015569A (zh) 2015-04-21 2022-02-08 通用自动化实验技术公司 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
JP2018518157A (ja) 2015-05-11 2018-07-12 イラミーナ インコーポレーテッド 治療薬剤の発見および分析のためのプラットフォーム
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
US9771575B2 (en) 2015-06-19 2017-09-26 Agilent Technologies, Inc. Methods for on-array fragmentation and barcoding of DNA samples
CN114350752A (zh) 2015-07-17 2022-04-15 纳米线科技公司 在横切面组织的用户定义区域中的基因表达的同时定量
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
CN108368522A (zh) 2015-10-20 2018-08-03 10X 基因组学有限公司 用于高通量单细胞遗传操作的方法和系统
US11111487B2 (en) * 2015-10-28 2021-09-07 Silicon Valley Scientific, Inc. Method and apparatus for encoding cellular spatial position information
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201803983UA (en) * 2015-11-19 2018-06-28 10X Genomics Inc Transformable tagging compositions, methods, and processes incorporating same
EP3384048B1 (en) 2015-12-04 2021-03-24 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
EP3387128A4 (en) * 2015-12-07 2019-12-04 Bio-Rad Laboratories, Inc. MULTIPLEXING IN PARTITIONS USING MICROPARTICLES
CN105567562B (zh) * 2015-12-30 2017-10-03 中国科学院合肥物质科学研究院 用于单细胞超声波基因导入的微流体系统及其导入方法
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
US20170283864A1 (en) * 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
US11162134B2 (en) * 2016-04-01 2021-11-02 Baylor College Of Medicine Methods of whole transcriptome amplification
US20190127789A1 (en) * 2016-04-19 2019-05-02 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017204294A1 (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN113151423A (zh) 2016-08-05 2021-07-23 生物辐射实验室股份有限公司 第二链引导
WO2018031631A1 (en) * 2016-08-10 2018-02-15 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic dna fragments
WO2018039338A1 (en) 2016-08-23 2018-03-01 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
ES2870639T3 (es) 2016-10-24 2021-10-27 Geneinfosec Inc Ocultación de información presente en los ácidos nucleicos
EP3538672A1 (en) 2016-11-08 2019-09-18 Cellular Research, Inc. Methods for cell label classification
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
WO2018089641A2 (en) * 2016-11-10 2018-05-17 The Scripps Research Institute Encode solid phase compound library with polynucleotide based barcoding
EP3555290B1 (en) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
JP7033602B2 (ja) 2017-01-27 2022-03-10 エフ.ホフマン-ラ ロシュ アーゲー ロングレンジ配列決定のためのバーコードを付けられたdna
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3391958B1 (en) 2017-04-19 2020-08-12 The Procter & Gamble Company Method of making surface-coated water-absorbing polymer particles in a microfluidic device
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
JP7197567B2 (ja) 2017-05-05 2022-12-27 スキピオ バイオサイエンス ハイドロゲル中の個別の生物学的単位を捕捉およびバーコード付与するための方法
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4215616A1 (en) 2017-05-18 2023-07-26 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
SG11201912824YA (en) 2017-06-20 2020-01-30 10X Genomics Inc Methods and systems for improved droplet stabilization
CN111094585A (zh) * 2017-08-01 2020-05-01 伊鲁米纳公司 用于核苷酸测序的水凝胶珠
US20210032693A1 (en) 2017-08-10 2021-02-04 Rootpath Genomics, Inc. Improved Method to Analyze Nucleic Acid Contents from Multiple Biological Particles
US10357771B2 (en) 2017-08-22 2019-07-23 10X Genomics, Inc. Method of producing emulsions
WO2019040546A1 (en) * 2017-08-23 2019-02-28 Roche Sequencing Solutions, Inc ENZYME SCREENING METHODS
US10636761B2 (en) * 2017-08-29 2020-04-28 Electronics And Telecommunications Reearch Institute Method of fabricating a semiconductor package
US10748643B2 (en) 2017-08-31 2020-08-18 10X Genomics, Inc. Systems and methods for determining the integrity of test strings with respect to a ground truth string
CN109486923B (zh) * 2017-09-11 2022-02-18 广州永诺生物科技有限公司 多重扩增子测序用引物系统、其应用以及测序文库的构建方法
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US11402400B2 (en) 2017-10-13 2022-08-02 Hewlett-Packard Development Company, L.P. Partition liquid into samples
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
EP3704247B1 (en) 2017-11-02 2023-01-04 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
JP7049103B2 (ja) * 2017-12-11 2022-04-06 株式会社日立製作所 単一細胞の網羅的3’末端遺伝子発現解析法
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides
WO2019148042A1 (en) * 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
EP4324962A2 (en) 2018-01-31 2024-02-21 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
KR20200121329A (ko) 2018-02-12 2020-10-23 나노스트링 테크놀로지스, 인크. 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019191900A1 (en) * 2018-04-03 2019-10-10 Burning Rock Biotech Compositions and methods for preparing nucleic acid libraries
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
DE102018207098A1 (de) * 2018-05-08 2019-11-14 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Nanostruktur-Sequenzierung von Nukleotidsträngen
US11654404B2 (en) 2018-05-18 2023-05-23 Massachusetts Institute Of Technology Light emitting droplets and related methods
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3841202B1 (en) 2018-08-20 2023-10-04 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
CN112805389A (zh) 2018-10-01 2021-05-14 贝克顿迪金森公司 确定5’转录物序列
EP3877520A1 (en) 2018-11-08 2021-09-15 Becton Dickinson and Company Whole transcriptome analysis of single cells using random priming
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
EP3923977A1 (en) 2019-02-12 2021-12-22 TheryCell GmbH Reverse immunosuppression
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3953489A1 (en) 2019-04-12 2022-02-16 Miltenyi Biotec B.V. & Co. KG Conjugates having an enzymatically releasable detection moiety and a barcode moiety
CN113454018A (zh) * 2019-04-30 2021-09-28 惠普发展公司,有限责任合伙企业 微流体装置
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
EP4018001A4 (en) * 2019-08-19 2023-09-13 Universal Sequencing Technology Corporation METHODS AND COMPOSITIONS FOR TRACKING THE ORIGIN OF NUCLEIC ACID FRAGMENTS FOR NUCLEIC ACID SEQUENCING
US11919000B2 (en) 2019-10-10 2024-03-05 1859, Inc. Methods and systems for microfluidic screening
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
CN114729393A (zh) 2019-11-15 2022-07-08 美天施生物科技有限两合公司 用于单细胞索引的颜色和条码化珠
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
CN114885610A (zh) 2019-12-23 2022-08-09 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
AU2021208466A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Single cell sequencing
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US11512337B2 (en) 2020-01-13 2022-11-29 Fluent Biosciences Inc. Emulsion based drug screening
US11827936B2 (en) 2020-01-13 2023-11-28 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
EP3859012A1 (en) * 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CN115461470A (zh) 2020-02-28 2022-12-09 10X基因组学有限公司 用于从组织中分离核和细胞的方法
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4121016A1 (en) 2020-03-16 2023-01-25 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
CN115916972A (zh) 2020-04-16 2023-04-04 10X基因组学有限公司 用于被固定样品的组合物和方法
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
CN116249785A (zh) 2020-06-02 2023-06-09 10X基因组学有限公司 用于抗原-受体的空间转录组学
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
EP4176437A1 (en) 2020-07-02 2023-05-10 10X Genomics, Inc. Systems and methods for detection of low-abundance molecular barcodes from a sequencing library
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US20220017892A1 (en) * 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
CN115997126A (zh) 2020-09-07 2023-04-21 美天施生物科技有限两合公司 具有可酶促释放的检测部分和条形码部分的缀合物
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4301870A1 (en) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2023014303A2 (en) * 2021-08-05 2023-02-09 Agency For Science, Technology And Research A method of amplification of a nucleic acid
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
KR102578493B1 (ko) 2023-04-26 2023-09-14 대한민국 적재용 선반

Family Cites Families (778)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1007137A (en) 1908-06-08 1911-10-31 Theodore D Palmer Cloth-pressing machine.
US1004111A (en) 1908-10-17 1911-09-26 Westinghouse Air Brake Co Fluid-pressure brake.
US1003026A (en) 1911-04-12 1911-09-12 Bernard Bartmann Bobbin-holder.
US1005998A (en) 1911-07-10 1911-10-17 Arthur Munchausen Berry-carrier.
US1005372A (en) 1911-08-11 1911-10-10 Albert Gay Plow-fender.
US2797149A (en) 1953-01-08 1957-06-25 Technicon International Ltd Methods of and apparatus for analyzing liquids containing crystalloid and non-crystalloid constituents
US3047367A (en) 1959-12-01 1962-07-31 Technicon Instr Automatic analysis with fluid segmentation
US3479141A (en) 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
US4124638A (en) 1977-09-12 1978-11-07 Hansen John N Solubilizable polyacrylamide gels containing disulfide cross-linkages
US4253846A (en) 1979-11-21 1981-03-03 Technicon Instruments Corporation Method and apparatus for automated analysis of fluid samples
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
DE3230289A1 (de) 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen Herstellung von pharmazeutischen oder kosmetischen dispersionen
US4582802A (en) 1983-09-30 1986-04-15 The United States Of America As Represented By The Department Of Health And Human Services Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers
JPS60227826A (ja) 1984-04-27 1985-11-13 Sogo Yatsukou Kk pHに応答するグラフトカプセル
KR890003947B1 (ko) * 1985-12-11 1989-10-13 가부시기가이샤 시마즈세이사구쇼 세포융합장치
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5418149A (en) 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4872895A (en) 1986-12-11 1989-10-10 American Telephone And Telegraph Company, At&T Bell Laboratories Method for fabricating articles which include high silica glass bodies
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5185099A (en) 1988-04-20 1993-02-09 Institut National De Recherche Chimique Appliquee Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US6176962B1 (en) 1990-02-28 2001-01-23 Aclara Biosciences, Inc. Methods for fabricating enclosed microchannel structures
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69213240T2 (de) 1991-07-04 1997-04-24 Immunodex K S Wasserlösliche reagenzien und konjugate auf polymerbasis, die vom divinylsulfon abgeleitete reste enthalten
DK0597960T3 (da) 1991-08-10 1999-09-13 Medical Res Council Behandling af cellepopulationer
EP0604552B1 (en) 1991-09-18 1997-02-12 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
AU3816993A (en) 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
CA2134475C (en) 1992-05-01 1999-07-06 Peter Wilding Polynucleotide amplification analysis using a microfabricated device
US5840865A (en) 1992-09-14 1998-11-24 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
US5897783A (en) 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
US5569364A (en) 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
WO1994018218A1 (en) 1993-02-01 1994-08-18 Seq, Ltd. Methods and apparatus for dna sequencing
WO1994019101A1 (en) 1993-02-16 1994-09-01 Alliance Pharmaceutical Corp. Method of microemulsifying fluorinated oils
WO1994023699A1 (en) 1993-04-19 1994-10-27 Medisorb Technologies International L.P. Long-acting treatment by slow-release delivery of antisense oligodeoxyribonucleotides from biodegradable microparticles
US5456986A (en) * 1993-06-30 1995-10-10 Carnegie Mellon University Magnetic metal or metal carbide nanoparticles and a process for forming same
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corp Ny Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
EP0711303B2 (en) 1993-07-30 2009-06-10 Affymax, Inc. Biotinylation of proteins
EP0723549B1 (en) 1993-08-30 2003-12-17 Promega Corporation Nucleic acid purification compositions and methods
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
US20030044777A1 (en) 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5558071A (en) 1994-03-07 1996-09-24 Combustion Electromagnetics, Inc. Ignition system power converter and controller
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
WO1995031726A1 (en) 1994-05-11 1995-11-23 Genera Technologies Limited Methods of capturing species from liquids and assay procedures
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
WO1996029629A2 (en) 1995-03-01 1996-09-26 President And Fellows Of Harvard College Microcontact printing on surfaces and derivative articles
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
HUP9900910A2 (hu) 1995-06-07 1999-07-28 Lynx Therapeutics, Inc. Oligonukleotid jelzések osztályozáshoz és azonosításhoz
ATE375402T1 (de) 1995-06-07 2007-10-15 Solexa Inc Oligonukleotid-tags zur sortierung und identifizierung
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6866760B2 (en) 1998-08-27 2005-03-15 E Ink Corporation Electrophoretic medium and process for the production thereof
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US6051377A (en) 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US5736332A (en) 1995-11-30 1998-04-07 Mandecki; Wlodek Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
WO1997039359A1 (en) 1996-04-15 1997-10-23 Dade International Inc. Apparatus and method for analysis
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
ATE206633T1 (de) 1996-07-15 2001-10-15 Calcitech Ltd Herstellung von pulvern
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6379929B1 (en) 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US20020172965A1 (en) 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US20050042625A1 (en) 1997-01-15 2005-02-24 Xzillion Gmbh & Co. Mass label linked hybridisation probes
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
CN1251609A (zh) 1997-02-12 2000-04-26 尤金·Y·查恩 分析聚合物的方法和产品
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US20030027203A1 (en) 1997-03-24 2003-02-06 Fields Robert E. Biomolecular processor
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
WO1998050583A1 (en) 1997-05-02 1998-11-12 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
CA2286601A1 (en) 1997-05-16 1998-11-26 Alberta Research Council Microfluidic system and methods of use
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
JP4294740B2 (ja) 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
US20040241759A1 (en) 1997-06-16 2004-12-02 Eileen Tozer High throughput screening of libraries
DK1496120T3 (da) 1997-07-07 2007-07-30 Medical Res Council In vitro-sorteringsmetode
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US20050037397A1 (en) 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US6368871B1 (en) 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
JP2001514906A (ja) 1997-08-15 2001-09-18 ハイセック,インコーポレーテッド 核酸種を検出または定量するための方法および組成物
WO1999014368A2 (en) 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
US20020092767A1 (en) 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6103537A (en) 1997-10-02 2000-08-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
EP1028970A1 (en) 1997-10-10 2000-08-23 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
EP1023464B1 (en) 1997-10-14 2017-07-26 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
EP1025434A4 (en) 1997-10-24 2006-10-18 Univ Northeastern MINIATURIZED MULTICHANNEL DEVICE FOR PREPARATORY SEPARATION WITH HIGH THROUGHPUT AND COMPREHENSIVE COLLECTION AND ANALYSIS
EP1036332B1 (en) 1997-12-04 2005-07-13 Amersham Biosciences UK Limited Multiple assay method
WO1999033963A1 (en) 1997-12-31 1999-07-08 Chiron Corporation Metastatic cancer regulated gene
AU765378B2 (en) 1998-02-19 2003-09-18 President And Fellows Of Harvard College Monovalent, multivalent, and multimeric MHC binding domain fusion proteins and conjugates, and uses therefor
AU3196099A (en) 1998-03-27 1999-10-18 President And Fellows Of Harvard College Systematic identification of essential genes by (in vitro) transposon mutagenesis
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
WO1999052708A1 (en) 1998-04-13 1999-10-21 Luminex Corporation Liquid labeling with fluorescent microparticles
US5997636A (en) 1998-05-01 1999-12-07 Instrumentation Technology Associates, Inc. Method and apparatus for growing crystals
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
EP2360271A1 (en) 1998-06-24 2011-08-24 Illumina, Inc. Decoding of array sensors with microspheres
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CA2347182C (en) 1998-10-13 2004-06-15 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6489096B1 (en) 1998-10-15 2002-12-03 Princeton University Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays
SE9803614L (sv) 1998-10-19 2000-04-20 Muhammed Mamoun Förfarande och anordning för framställning av nanopartiklar
WO2000026412A1 (en) 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
US6569631B1 (en) 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US6635419B1 (en) 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
US20030027214A1 (en) 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
ATE556149T1 (de) 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
US7615373B2 (en) 1999-02-25 2009-11-10 Virginia Commonwealth University Intellectual Property Foundation Electroprocessed collagen and tissue engineering
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
EP1923472B1 (en) 1999-04-20 2012-04-11 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
JP2002542781A (ja) 1999-04-28 2002-12-17 ザ、ボード、オブ、トラスティーズ、オブ、ザ、リーランド、スタンフォード、ジュニア、ユニバーシティ P因子由来ベクター及びその使用法
CA2373537A1 (en) 1999-05-12 2000-11-16 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6846622B1 (en) 1999-05-26 2005-01-25 Oregon Health & Science University Tagged epitope protein transposable element
US20030124509A1 (en) 1999-06-03 2003-07-03 Kenis Paul J.A. Laminar flow patterning and articles made thereby
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
WO2001013086A2 (en) 1999-08-13 2001-02-22 Brandeis University Detection of nucleic acids
AU6788100A (en) 1999-08-20 2001-03-19 Luminex Corporation Liquid array technology
EP1208238B1 (en) 1999-08-27 2008-11-12 Matrix Technologies Corporation Methods of immobilizing ligands on solid supports
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
IL149001A0 (en) 1999-10-13 2002-11-10 Signature Bioscience Inc System and method for detecting and identifying molecular events in test sample
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
JP4721603B2 (ja) 1999-11-08 2011-07-13 栄研化学株式会社 変異および/または多型の検出方法
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US20010051348A1 (en) 2000-01-28 2001-12-13 Lee Chee Wee Novel ligands and methods for preparing same
ATE382858T1 (de) 2000-02-23 2008-01-15 Caliper Life Sciences Inc Mehrfach-reservoir-drucksteuersystem
EP1261689A4 (en) 2000-02-23 2005-06-29 Zyomyx Inc CHIPS WITH INCREASED SAMPLE SURFACE
IL134830A0 (en) 2000-03-01 2001-05-20 Chay 13 Medical Res Group N V Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them
AU2001252201A1 (en) 2000-03-14 2001-09-24 Amylin Pharmaceuticals, Inc. Effects of glucagon-like peptide-1 (7-36) on antro-pyloro-duodenal motility
JP4927287B2 (ja) 2000-03-31 2012-05-09 マイクロニックス、インコーポレーテッド タンパク質の結晶化のマイクロ流動体装置
US20020001856A1 (en) 2000-04-06 2002-01-03 Chow Andrea W. Methods and devices for achieving long incubation times in high-throughput systems
EP2278030B1 (en) 2000-04-10 2017-05-24 Taxon Biosciences, Inc. Methods for the survey and genetic analysis of populations
US6481453B1 (en) 2000-04-14 2002-11-19 Nanostream, Inc. Microfluidic branch metering systems and methods
US6800298B1 (en) 2000-05-11 2004-10-05 Clemson University Biological lubricant composition and method of applying lubricant composition
US20060008799A1 (en) 2000-05-22 2006-01-12 Hong Cai Rapid haplotyping by single molecule detection
US20020003001A1 (en) 2000-05-24 2002-01-10 Weigl Bernhard H. Surface tension valves for microfluidic applications
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US6632606B1 (en) 2000-06-12 2003-10-14 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
ES2259666T3 (es) 2000-06-21 2006-10-16 Bioarray Solutions Ltd Analisis molecular de multiples analitos usando series de particulas aleatorias con especificidad de aplicacion.
WO2002011888A2 (en) 2000-08-07 2002-02-14 Nanostream, Inc. Fluidic mixer in microfluidic system
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
ATE448875T1 (de) 2000-09-14 2009-12-15 Caliper Life Sciences Inc Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen
AU2001290879A1 (en) 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
EP1322936A2 (en) 2000-10-03 2003-07-02 California Institute Of Technology Microfluidic devices and methods of use
CA2393374A1 (en) 2000-10-10 2002-04-18 Diversa Corporation High throughput or capillary-based screening for a bioactivity or biomolecule
JP2002155305A (ja) 2000-11-14 2002-05-31 Akira Kawasaki 単分散粒子の製造装置及び単分散粒子の製造方法及びその製造方法で製造された単分散粒子
US6492154B2 (en) 2001-01-31 2002-12-10 Applera Corporation Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof
CA2332186A1 (en) 2001-02-08 2002-08-08 Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Ture And Agri-Food Canada Replicative in vivo gene targeting
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
ATE312076T1 (de) 2001-02-22 2005-12-15 Anika Therapeutics Inc Thiol-modifizierte hyaluronan-derivate
DE60211857T2 (de) 2001-02-23 2006-12-21 Japan Science And Technology Agency, Kawaguchi Verfahren zur herstellung einer emulsion und vorrichtung dafür
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
US20150329617A1 (en) 2001-03-14 2015-11-19 Dynal Biotech Asa Novel MHC molecule constructs, and methods of employing these constructs for diagnosis and therapy, and uses of MHC molecules
JP3949056B2 (ja) 2001-04-03 2007-07-25 マイクロニクス, インコーポレイテッド スプリット集中サイトメーター
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US20030027221A1 (en) * 2001-04-06 2003-02-06 Scott Melissa E. High-throughput screening assays by encapsulation
US7572642B2 (en) 2001-04-18 2009-08-11 Ambrigen, Llc Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns
JP4194934B2 (ja) 2001-05-26 2008-12-10 ワン セル システムズ インコーポレイテッド カプセル化細胞によるタンパク質の分泌法
US6880576B2 (en) 2001-06-07 2005-04-19 Nanostream, Inc. Microfluidic devices for methods development
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7682353B2 (en) 2001-06-29 2010-03-23 Coloplast A/S Catheter device
US7077152B2 (en) 2001-07-07 2006-07-18 Nanostream, Inc. Microfluidic metering systems and methods
EP1427746A4 (en) 2001-07-20 2005-09-28 California Inst Of Techn SYSTEMS FOR THE EXPRESSION OF PROTEINS AND NUCLEIC ACIDS
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
WO2003031591A2 (en) 2001-10-10 2003-04-17 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
WO2003044221A1 (en) 2001-10-19 2003-05-30 West Virginia University Research Corporation Microfluidic system for proteome analysis
US20030149307A1 (en) 2001-10-24 2003-08-07 Baxter International Inc. Process for the preparation of polyethylene glycol bis amine
AU2002365140A1 (en) 2001-10-26 2003-07-15 Aclara Biosciences Inc. System and method for injection molded micro-replication of micro-fluidic substrates
US20030182068A1 (en) 2001-10-30 2003-09-25 Battersby Bronwyn J. Device and methods for directed synthesis of chemical libraries
US7262056B2 (en) 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP1456409B1 (en) 2001-11-28 2010-02-24 Bio-Rad Laboratories, Inc. Parallel polymorphism scoring by amplification and error correction
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
AU2003210438A1 (en) 2002-01-04 2003-07-24 Board Of Regents, The University Of Texas System Droplet-based microfluidic oligonucleotide synthesis engine
AU2003202026A1 (en) 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
KR100459870B1 (ko) 2002-02-22 2004-12-04 한국과학기술원 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
CN1656234B (zh) * 2002-03-20 2012-02-01 创新生物公司 包囊化核酸扩增反应混合物的微胶囊及其作为反应隔间进行平行反应的用途
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
EP2282214B1 (en) 2002-05-09 2022-10-05 The University of Chicago Device and method for pressure-driven plug transport and reaction
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US7927791B2 (en) 2002-07-24 2011-04-19 Ptc Therapeutics, Inc. Methods for identifying small molecules that modulate premature translation termination and nonsense mediated mRNA decay
IL151660A0 (en) 2002-09-09 2003-04-10 Univ Ben Gurion Method for isolating and culturing unculturable microorganisms
ES2315397T3 (es) 2002-09-30 2009-04-01 F. Hoffmann-La Roche Ag Oligonucleotidos para obtener el genotipo del gen de timidilato sintasa.
US20040081962A1 (en) 2002-10-23 2004-04-29 Caifu Chen Methods for synthesizing complementary DNA
US6979713B2 (en) 2002-11-25 2005-12-27 3M Innovative Properties Company Curable compositions and abrasive articles therefrom
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US20040248299A1 (en) 2002-12-27 2004-12-09 Sumedha Jayasena RNA interference
DE602004021902D1 (de) 2003-01-17 2009-08-20 Univ Boston Haplotypanalyse
US7244567B2 (en) 2003-01-29 2007-07-17 454 Life Sciences Corporation Double ended sequencing
DK1594973T3 (da) 2003-02-10 2012-02-13 Max Delbrueck Centrum Transposonbaseret målretningssystem
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7316903B2 (en) 2003-03-28 2008-01-08 United States Of America As Represented By The Department Of Health And Human Services Detection of nucleic acid sequence variations using phase Mu transposase
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
DK1613346T3 (da) * 2003-04-04 2013-01-07 Pah Usa 15 Llc Mikrofluidiske olie-i-vand-emulsioner og vaccinesammensætninger
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
CA2784762A1 (en) 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
EP1629286A1 (en) 2003-05-16 2006-03-01 Global Technologies (NZ) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
DE112004001376D2 (de) 2003-05-19 2006-04-13 Knoell Hans Forschung Ev Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten und zum zudosieren von Reaktionsflüssigkeiten zu in Separationsmedium eingebetteten Flüssigkeitskompartimenten
WO2004105734A1 (en) 2003-05-28 2004-12-09 Valorisation Recherche, Societe En Commandite Method of preparing microcapsules
GB0313170D0 (en) 2003-06-09 2003-07-16 Qinetiq Ltd Method and apparatus for spore disruption and/or detection
JP2007503597A (ja) 2003-06-13 2007-02-22 ザ ジェネラル ホスピタル コーポレーション 血液から赤血球および血小板をサイズに基づいて除去するための微少流体システム
GB2403475B (en) 2003-07-01 2008-02-06 Oxitec Ltd Stable integrands
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP2662135A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for mixing droplets in a microchannel
JP4988345B2 (ja) 2003-09-04 2012-08-01 ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプレゼンティッド・バイ・ザ・デパートメント・オブ・ヴェテランズ・アフェアーズ 眼用ハイドロゲルナノコンポジット
WO2005023427A1 (en) 2003-09-05 2005-03-17 Stokes Bio Limited A microfluidic analysis system
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
WO2005028646A1 (ja) 2003-09-22 2005-03-31 Riken 効率的なdna逆位反復構造の調製方法
KR101111231B1 (ko) 2003-09-25 2012-02-17 비바리스 마이크로 웰 어레이 칩 및 그 제조방법
JP2007512811A (ja) 2003-11-10 2007-05-24 インベスチゲン, インコーポレイテッド 検出のための核酸を調製する方法
WO2005049787A2 (en) 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
US20050136417A1 (en) 2003-12-19 2005-06-23 Affymetrix, Inc. Amplification of nucleic acids
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
ES2432040T3 (es) 2004-01-28 2013-11-29 454 Life Sciences Corporation Amplificación de ácido nucleico con emulsión de flujo continuo
US20050181379A1 (en) 2004-02-18 2005-08-18 Intel Corporation Method and device for isolating and positioning single nucleic acid molecules
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
US8741192B2 (en) 2004-03-23 2014-06-03 Japan Science And Technology Agency Method and device for producing micro-droplets
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2005099419A2 (en) 2004-04-13 2005-10-27 President And Fellows Of Harvard College Manipulation and/or detection of biological samples or other objects
US20050250147A1 (en) 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
US20080268507A1 (en) 2004-05-25 2008-10-30 Airbus Deutschland Gmbh Recombinant Dna Nicking Endonuclease and Uses Thereof
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US7968085B2 (en) 2004-07-05 2011-06-28 Ascendis Pharma A/S Hydrogel formulations
CN1648671B (zh) * 2005-02-06 2012-09-26 成都夸常医学工业有限公司 多反应器分析芯片检测方法和分析芯片及检测装置
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
US20080268431A1 (en) 2004-09-14 2008-10-30 Jin-Ho Choy Information Code System Using Dna Sequences
US7892731B2 (en) 2004-10-01 2011-02-22 Radix Biosolutions, Ltd. System and method for inhibiting the decryption of a nucleic acid probe sequence used for the detection of a specific nucleic acid
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US9492400B2 (en) 2004-11-04 2016-11-15 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
WO2006051552A2 (en) 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
US7329493B2 (en) 2004-12-22 2008-02-12 Asiagen Corporation One-tube nested PCR for detecting Mycobacterium tuberculosis
EP2418018B1 (en) 2004-12-23 2013-05-22 Abbott Point of Care Inc. Methods for the separation nucleic acids
US20080213593A1 (en) 2005-01-21 2008-09-04 President And Fellows Of Harvard College Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
AU2006214085A1 (en) 2005-02-18 2006-08-24 Canon U.S. Life Sciences, Inc. Devices and methods for identifying genomic DNA of organisms
EP1867702B1 (en) * 2005-02-21 2011-09-28 Kagoshima University Method for purifying biodiesel fuel
EP2248578B1 (en) 2005-03-04 2012-06-06 President and Fellows of Harvard College Method for forming multiple emulsions
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
JP2006289250A (ja) 2005-04-08 2006-10-26 Kao Corp マイクロミキサー及びそれを用いた流体混合方法
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US8445194B2 (en) 2005-06-15 2013-05-21 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
US8828209B2 (en) 2005-06-22 2014-09-09 The Research Foundation For The State University Of New York Massively parallel 2-dimensional capillary electrophoresis
WO2007002567A2 (en) 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
ATE537254T1 (de) 2005-07-05 2011-12-15 Chemo Sero Therapeut Res Inst Mutanter transposonvektor und verwendung davon
JP5051490B2 (ja) 2005-07-08 2012-10-17 独立行政法人産業技術総合研究所 マクロ生体材料を内包する無機マイクロカプセルおよびその製造方法
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
FR2888912B1 (fr) 2005-07-25 2007-08-24 Commissariat Energie Atomique Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif
ATE510930T1 (de) 2005-08-02 2011-06-15 Rubicon Genomics Inc Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
DE102005037401B4 (de) 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
WO2007024840A2 (en) 2005-08-22 2007-03-01 Critical Therapeutics, Inc. Method of quantitating nucleic acids by flow cytometry microparticle-based array
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
JP2007074967A (ja) 2005-09-13 2007-03-29 Canon Inc 識別子プローブ及びそれを用いた核酸増幅方法
CN101523156A (zh) 2005-09-16 2009-09-02 加利福尼亚大学董事会 用于分析物检测的比色生物条形码扩增测定
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007133831A2 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US20070111241A1 (en) 2005-10-14 2007-05-17 Nezih Cereb System and method for accessing, tracking, and editing sequence analysis and software to accomplish the same
US7709544B2 (en) 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
US20070190543A1 (en) * 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
US20070134277A1 (en) 2005-12-09 2007-06-14 Children's Medical Center Corporation Pharmaceutical formulation for sulfur-containing drugs in liquid dosage forms
US20070141584A1 (en) 2005-12-20 2007-06-21 Roberts Douglas N Methods for assessment of native chromatin on microarrays
US7932037B2 (en) 2007-12-05 2011-04-26 Perkinelmer Health Sciences, Inc. DNA assays using amplicon probes on encoded particles
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
EP1987162A4 (en) 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
EP2263787A3 (en) 2006-01-27 2012-02-22 President and Fellows of Harvard College Fluidic droplet coalescence
ES2595373T3 (es) 2006-02-02 2016-12-29 The Board Of Trustees Of The Leland Stanford Junior University Prueba genética no invasiva mediante análisis digital
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603251D0 (en) 2006-02-17 2006-03-29 Isis Innovation DNA conformation
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
JP4921829B2 (ja) 2006-03-30 2012-04-25 株式会社東芝 微粒子の製造装置、乳化剤保持部、微粒子の製造方法および分子膜の製造方法
US20090181864A1 (en) 2006-03-31 2009-07-16 Nam Trung Nguyen Active control for droplet-based microfluidics
JP2009538123A (ja) 2006-04-19 2009-11-05 アプライド バイオシステムズ, エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US7811603B2 (en) 2006-05-09 2010-10-12 The Regents Of The University Of California Microfluidic device for forming monodisperse lipoplexes
JP2010506136A (ja) 2006-05-11 2010-02-25 レインダンス テクノロジーズ, インコーポレイテッド 微小流体デバイス
ES2620398T3 (es) 2006-05-22 2017-06-28 Nanostring Technologies, Inc. Sistemas y métodos para analizar nanoindicadores
RU2321638C2 (ru) 2006-05-23 2008-04-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Способ изготовления многофункционального мультичипа, мультичип для последовательного или параллельного скрининга биополимеров, способ анализа биополимеров и набор для осуществления способа
EP2029781A4 (en) 2006-05-26 2010-06-30 Althea Technologies Inc BIOCHEMICAL ANALYSIS OF PARTITIONED CELLS
FR2901717A1 (fr) 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
AU2007260676A1 (en) 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
WO2007149432A2 (en) 2006-06-19 2007-12-27 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
US20080076909A1 (en) 2006-06-30 2008-03-27 Applera Corporation Emulsion pcr and amplicon capture
EP1878501A1 (en) 2006-07-14 2008-01-16 Roche Diagnostics GmbH Instrument for heating and cooling
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
EP3536396B1 (en) 2006-08-07 2022-03-30 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
US9278321B2 (en) 2006-09-06 2016-03-08 Canon U.S. Life Sciences, Inc. Chip and cartridge design configuration for performing micro-fluidic assays
WO2008037082A1 (en) 2006-09-25 2008-04-03 Archer-Daniels-Midland Company Superabsorbent surface-treated carboxyalkylated polysaccharides and process for producing same
US7935518B2 (en) 2006-09-27 2011-05-03 Alessandra Luchini Smart hydrogel particles for biomarker harvesting
US20080166720A1 (en) 2006-10-06 2008-07-10 The Regents Of The University Of California Method and apparatus for rapid nucleic acid analysis
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US8765076B2 (en) 2006-11-14 2014-07-01 Handylab, Inc. Microfluidic valve and method of making same
ES2679996T3 (es) 2006-11-15 2018-09-03 Biospherex Llc Secuenciación multi-etiqueta y análisis ecogenómico
CN101583879A (zh) * 2006-11-20 2009-11-18 纳提维斯公司 利用低频信号转导体外系统或者哺乳动物系统的设备和方法
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
WO2008072540A1 (ja) 2006-12-13 2008-06-19 National University Corporation Nagoya University Tol1因子のトランスポザーゼ及びそれを用いたDNA導入システム
CA2672315A1 (en) 2006-12-14 2008-06-26 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US8338166B2 (en) * 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
JP2008167722A (ja) 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
US7844658B2 (en) 2007-01-22 2010-11-30 Comcast Cable Holdings, Llc System and method for providing an application to a device
US20080176768A1 (en) 2007-01-23 2008-07-24 Honeywell Honeywell International Hydrogel microarray with embedded metal nanoparticles
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20080228268A1 (en) 2007-03-15 2008-09-18 Uluru, Inc. Method of Formation of Viscous, Shape Conforming Gels and Their Uses as Medical Prosthesis
US7943330B2 (en) 2007-03-23 2011-05-17 Academia Sinica Tailored glycoproteomic methods for the sequencing, mapping and identification of cellular glycoproteins
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
CN101293191B (zh) 2007-04-25 2011-11-09 中国科学院过程工程研究所 一种琼脂糖凝胶微球的制备方法
WO2008135512A2 (en) 2007-05-02 2008-11-13 Jerzy Paszkowski Dna amplification method
US20080295909A1 (en) 2007-05-24 2008-12-04 Locascio Laurie E Microfluidic Device for Passive Sorting and Storage of Liquid Plugs Using Capillary Force
WO2008150432A1 (en) 2007-06-01 2008-12-11 454 Life Sciences Corporation System and meth0d for identification of individual samples from a multiplex mixture
US8476382B2 (en) 2007-06-05 2013-07-02 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009005680A1 (en) 2007-06-29 2009-01-08 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
WO2009011808A1 (en) 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US20130084243A1 (en) 2010-01-27 2013-04-04 Liliane Goetsch Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders
EP2179285A4 (en) 2007-08-15 2010-08-18 Opgen Inc METHOD, SYSTEM AND SOFTWARE ARRANGEMENT FOR COMPARATIVE ANALYSIS AND PHYLOGENIA WITH OPTICAL TOTAL GENERIC CARD
US8563527B2 (en) 2007-08-20 2013-10-22 Pharmain Corporation Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
WO2009048532A2 (en) 2007-10-05 2009-04-16 President And Fellows Of Harvard College Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation
US20090099040A1 (en) 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
US20100086914A1 (en) 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
US8334013B2 (en) 2007-11-02 2012-12-18 Stc.Unm Mesoporous metal oxide microspheres and method for forming same
WO2009061372A1 (en) 2007-11-02 2009-05-14 President And Fellows Of Harvard College Systems and methods for creating multi-phase entities, including particles and/or fluids
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20110008775A1 (en) 2007-12-10 2011-01-13 Xiaolian Gao Sequencing of nucleic acids
US7771944B2 (en) 2007-12-14 2010-08-10 The Board Of Trustees Of The University Of Illinois Methods for determining genetic haplotypes and DNA mapping
CN101946010B (zh) 2007-12-21 2014-08-20 哈佛大学 用于核酸测序的系统和方法
EP3699291A1 (en) 2008-01-17 2020-08-26 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
KR20090081260A (ko) 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
CA2714630A1 (en) 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
JP5468271B2 (ja) 2008-02-08 2014-04-09 花王株式会社 微粒子分散液の製造方法
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
CN101918597B (zh) 2008-03-11 2013-09-18 国立癌中心 应用snp阵列测量染色体、基因或特定核苷酸序列拷贝数的方法
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
DE102008025656B4 (de) 2008-05-28 2016-07-28 Genxpro Gmbh Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung
GB0810051D0 (en) 2008-06-02 2008-07-09 Oxford Biodynamics Ltd Method of diagnosis
EP2303246A1 (en) 2008-06-05 2011-04-06 President and Fellows of Harvard College Polymersomes, colloidosomes, liposomes, and other species associated with fluidic droplets
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
WO2010004018A2 (en) 2008-07-11 2010-01-14 Eth Zurich Degradable microcapsules
US10722562B2 (en) 2008-07-23 2020-07-28 Immudex Aps Combinatorial analysis and repair
US20100062494A1 (en) 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US20110159499A1 (en) 2009-11-25 2011-06-30 Quantalife, Inc. Methods and compositions for detecting genetic material
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
AU2009296734B2 (en) 2008-09-25 2016-02-18 Cephalon Llc Liquid formulations of bendamustine
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
DK2376517T3 (da) 2008-10-24 2013-02-11 Epict Technologies Corp Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer
US20100113296A1 (en) 2008-11-05 2010-05-06 Joel Myerson Methods And Kits For Nucleic Acid Analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US20100203647A1 (en) 2008-11-21 2010-08-12 The Rockefeller University Chemical Reporters of Protein Acylation
SG171916A1 (en) 2008-12-02 2011-07-28 Bio Rad Laboratories Chromatin structure detection
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
WO2010075570A2 (en) 2008-12-24 2010-07-01 New York University Methods, computer-accessible medium, and systems for score-driven whole-genome shotgun sequence assemble
KR101065807B1 (ko) 2009-01-23 2011-09-19 충남대학교산학협력단 액적 기반의 미세유체 칩을 이용한 마이크로 캡슐 제조방법
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010104597A2 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Scale-up of microfluidic devices
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
DK2414548T3 (da) 2009-03-30 2015-12-21 Illumina Inc Genekspressionsanalyse i enkeltceller
EP2789694A1 (en) 2009-04-02 2014-10-15 Fluidigm Corporation Microfluidic device with reaction product recovery system
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2427572B1 (en) 2009-05-01 2013-08-28 Illumina, Inc. Sequencing methods
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
FR2945545B1 (fr) 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
FR2945819B1 (fr) 2009-05-19 2011-06-17 Commissariat Energie Atomique Dispositif et procede d'isolement de cibles biologiques ou chimiques
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
CN102459592B (zh) 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
AU2010266010B2 (en) 2009-06-26 2015-08-20 President And Fellows Of Harvard College Fluid injection
US20110028412A1 (en) 2009-08-03 2011-02-03 Cappellos, Inc. Herbal enhanced analgesic formulations
US20110033548A1 (en) 2009-08-05 2011-02-10 E.I. Du Pont De Nemours And Company Degradable crosslinked aminated dextran microspheres and methods of use
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
RU2552215C2 (ru) 2009-09-01 2015-06-10 Конинклейке Филипс Электроникс Н.В. Устройство и способ отбора нуклеиновых кислот с помощью микроматриц
JP6155418B2 (ja) 2009-09-02 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 多重エマルジョンの合体による、流体を混合するためのシステム
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
CA2778816C (en) 2009-10-27 2018-07-31 President And Fellows Of Harvard College Droplet creation techniques
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
JP5823405B2 (ja) 2009-11-04 2015-11-25 ザ ユニバーシティ オブ ブリティッシュ コロンビア 核酸含有脂質粒子および関連方法
CN102985552B (zh) * 2009-11-25 2016-02-17 伯乐生命医学产品有限公司 用于检测遗传物质的方法和组合物
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2513333B1 (en) 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
EP3392349A1 (en) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digital analyte analysis
JP5901046B2 (ja) 2010-02-19 2016-04-06 国立大学法人 千葉大学 OATP1B3mRNAの新規な選択的スプライシングバリアント
US20110257889A1 (en) 2010-02-24 2011-10-20 Pacific Biosciences Of California, Inc. Sequence assembly and consensus sequence determination
EP2539450B1 (en) 2010-02-25 2016-02-17 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US10500156B2 (en) 2010-03-24 2019-12-10 Northeastern University Multi-compartmental macrophage delivery
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
FR2958186A1 (fr) 2010-03-30 2011-10-07 Ecole Polytech Dispositif de formation de gouttes dans un circuit microfluide.
US9255291B2 (en) 2010-05-06 2016-02-09 Bioo Scientific Corporation Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
US20110287947A1 (en) 2010-05-18 2011-11-24 University Of Southern California Tethered Conformation Capture
CN105194665A (zh) 2010-05-26 2015-12-30 西莱克塔生物科技公司 具有不偶合的佐剂的纳米载体组合物
US20120000777A1 (en) 2010-06-04 2012-01-05 The Regents Of The University Of California Devices and methods for forming double emulsion droplet compositions and polymer particles
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
US8703493B2 (en) 2010-06-15 2014-04-22 Src, Inc. Location analysis using fire retardant-protected nucleic acid-labeled tags
US20120003657A1 (en) 2010-07-02 2012-01-05 Samuel Myllykangas Targeted sequencing library preparation by genomic dna circularization
WO2012012037A1 (en) * 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
CN103202812B (zh) 2010-08-09 2015-10-28 南京大学 一种制备用于体内递送药理活性物质的蛋白纳米粒的方法
WO2012019765A1 (en) 2010-08-10 2012-02-16 European Molecular Biology Laboratory (Embl) Methods and systems for tracking samples and sample combinations
US20130203675A1 (en) 2010-09-16 2013-08-08 Joseph M. DeSimone Asymmetric biofunctional silyl monomers and particles thereof as prodrugs and delivery vehicles for pharmaceutical, chemical and biological agents
CN102409048B (zh) * 2010-09-21 2013-10-23 深圳华大基因科技服务有限公司 一种基于高通量测序的dna标签文库构建方法
WO2012038839A2 (en) 2010-09-21 2012-03-29 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
US9187783B2 (en) 2010-10-04 2015-11-17 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US9999886B2 (en) 2010-10-07 2018-06-19 The Regents Of The University Of California Methods and systems for on demand droplet generation and impedance based detection
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
EP2625295B1 (en) 2010-10-08 2019-03-13 President and Fellows of Harvard College High-throughput immune sequencing
CN102050953B (zh) 2010-10-18 2012-11-07 武汉理工大学 一种可还原降解超分子水凝胶的制备方法
WO2012055929A1 (en) 2010-10-26 2012-05-03 Illumina, Inc. Sequencing methods
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
DE202011110979U1 (de) 2010-11-01 2017-12-04 Bio-Rad Laboratories, Inc. System zum Bilden von Emulsionen
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CN102115789B (zh) * 2010-12-15 2013-03-13 厦门大学 用于第二代高通量测序的核酸标签及其设计方法
US20140057799A1 (en) 2010-12-16 2014-02-27 Gigagen System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
WO2012088348A2 (en) 2010-12-23 2012-06-28 Sequenom, Inc. Fetal genetic variation detection
WO2012100216A2 (en) 2011-01-20 2012-07-26 Knome, Inc. Methods and apparatus for assigning a meaningful numeric value to genomic variants, and searching and assessing same
GB201101429D0 (en) 2011-01-27 2011-03-16 Biocompatibles Uk Ltd Drug delivery system
US8765455B2 (en) * 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
AU2012212148B8 (en) * 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
JP2014506465A (ja) * 2011-02-09 2014-03-17 バイオ−ラド ラボラトリーズ,インコーポレイティド 核酸の分析
WO2012109604A1 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP3412778A1 (en) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
CA2824431A1 (en) 2011-02-25 2012-08-30 Illumina, Inc. Methods and systems for haplotype determination
WO2012116250A1 (en) 2011-02-25 2012-08-30 University Of Massachusetts Medical School Monomers and polymers for functional polycarbonates and poly (ester-carbonates) and peg-co-polycarbonate hydrogels
EP2681196B1 (en) 2011-03-04 2015-09-09 Life Technologies Corporation Compounds and methods for conjugation of biomolecules
WO2012122555A2 (en) 2011-03-09 2012-09-13 Lawrence Ganeshalingam Biological data networks and methods therefor
WO2012129187A1 (en) * 2011-03-18 2012-09-27 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
GB2489714B (en) 2011-04-05 2013-11-06 Tracesa Ltd Fluid Identification Method
CN107368705B (zh) 2011-04-14 2021-07-13 完整基因有限公司 分析生物体的基因组dna的方法和计算机系统
AU2012249759A1 (en) 2011-04-25 2013-11-07 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP2702170B1 (en) 2011-04-28 2015-12-16 Life Technologies Corporation Methods and compositions for multiplex pcr
SI3415619T1 (sl) 2011-04-28 2021-04-30 The Board of Trustees of the Leland Stanford Junior University Office of the General Counsel Building 170, Third Floor, Main Quad Identifikacija polinukleotidov, povezanih z vzorcem
EP2705156B1 (en) 2011-05-05 2015-08-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Linear dna amplification
US20140227706A1 (en) * 2011-05-16 2014-08-14 Dna Chip Research Inc. Method for assessing progression of clinical state of malignant neoplasm by quantitative detection of DNA in blood
JP5993940B2 (ja) 2011-05-16 2016-09-21 コーニンクレッカ フィリップス エヌ ヴェKoninklijke Philips N.V. 生体直交型薬物活性化
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
JP6122843B2 (ja) 2011-05-23 2017-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重エマルジョンを含むエマルジョンの制御
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9150916B2 (en) 2011-06-24 2015-10-06 Beat Christen Compositions and methods for identifying the essential genome of an organism
US8927218B2 (en) 2011-06-27 2015-01-06 Flir Systems, Inc. Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
US8975302B2 (en) 2011-07-07 2015-03-10 Life Technologies Corporation Polymer particles, nucleic acid polymer particles and methods of making and using the same
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US9605304B2 (en) 2011-07-20 2017-03-28 The Hong Kong Polytechnic University Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation
US20130189700A1 (en) * 2011-07-25 2013-07-25 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
WO2013020089A2 (en) 2011-08-04 2013-02-07 Sage Science, Inc. Systems and methods for processing fluids
US10364464B2 (en) 2011-08-08 2019-07-30 The Broad Institute, Inc. Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
US8846883B2 (en) 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
WO2013035114A1 (en) 2011-09-08 2013-03-14 Decode Genetics Ehf Tp53 genetic variants predictive of cancer
GB2496016B (en) 2011-09-09 2016-03-16 Univ Leland Stanford Junior Methods for obtaining a sequence
CN103945931B (zh) 2011-09-26 2017-03-22 基因技术股份公司 高效的小体积核酸合成
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
WO2013055955A1 (en) 2011-10-12 2013-04-18 Complete Genomics, Inc. Identification of dna fragments and structural variations
US9469874B2 (en) 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US20130109576A1 (en) 2011-10-28 2013-05-02 Anthony P. Shuber Methods for detecting mutations
JP2014534771A (ja) 2011-11-04 2014-12-18 インテル コーポレイション 下りリンク協調マルチポイント通信の構成のためのシグナリング
WO2013072790A1 (en) 2011-11-16 2013-05-23 International Business Machines Corporation Microfluidic device with deformable valve
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
CN104245917B (zh) 2011-12-03 2017-07-21 Emd密理博公司 用于微流体细胞培养的微型培育系统和方法
US10227639B2 (en) 2011-12-22 2019-03-12 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2013096643A1 (en) 2011-12-23 2013-06-27 Gigagen Methods and apparatuses for droplet mixing
RU2643034C2 (ru) 2012-02-09 2018-01-30 Лайф Текнолоджис Корпорейшн Гидрофильные полимерные частицы и способы их получения
CN104271768A (zh) 2012-02-14 2015-01-07 约翰霍普金斯大学 miRNA分析方法
CA2864287C (en) 2012-02-15 2021-01-12 Wisconsin Alumni Research Foundation Dithioamine reducing agents
EP3854873A1 (en) 2012-02-17 2021-07-28 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9176031B2 (en) 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
NO2694769T3 (da) 2012-03-06 2018-03-03
US9552458B2 (en) 2012-03-16 2017-01-24 The Research Institute At Nationwide Children's Hospital Comprehensive analysis pipeline for discovery of human genetic variation
US20150125904A1 (en) 2012-03-30 2015-05-07 Massachusetts Institute Of Technology Probe incorporation mediated by enzymes
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
CA2870160C (en) 2012-04-16 2021-08-24 Biological Dynamics, Inc. Nucleic acid sample preparation
US20130296173A1 (en) 2012-04-23 2013-11-07 Complete Genomics, Inc. Pre-anchor wash
EP3514243B1 (en) 2012-05-21 2022-08-17 The Scripps Research Institute Methods of sample preparation
ES2774165T3 (es) 2012-06-15 2020-07-17 Univ Texas Secuenciación de alto rendimiento de múltiples transcritos de una única célula
SG11201500313YA (en) 2012-07-24 2015-02-27 Sequenta Inc Single cell analysis using sequence tags
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
CN110643681A (zh) 2012-08-13 2020-01-03 加利福尼亚大学董事会 用于检测生物组分的方法和系统
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378322A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014047561A1 (en) * 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
FR2996544B1 (fr) 2012-10-08 2015-03-13 Ecole Polytech Circuit microfluidique permettant la mise en contact de gouttes de plusieurs fluides, et procede microfluidique correspondant.
CN104838014B (zh) 2012-10-15 2017-06-30 生命技术公司 用于标靶核酸富集的组合物、方法、系统和试剂盒
EP2914745B1 (en) 2012-11-05 2017-09-06 Rubicon Genomics, Inc. Barcoding nucleic acids
EP2917366B1 (en) 2012-11-06 2017-08-02 Oxford Nanopore Technologies Limited Quadruplex method
US20140127688A1 (en) 2012-11-07 2014-05-08 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
US20150299772A1 (en) 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
PT2921557T (pt) 2012-12-12 2016-10-19 Massachusetts Inst Technology Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2749653A1 (en) 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Molecular coding for analysis of composition of macromolecules and molecular complexes
EP2752664A1 (en) 2013-01-07 2014-07-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Label-free method for the detection of analytes
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2994749A4 (en) 2013-01-17 2017-07-19 Edico Genome Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
KR101984699B1 (ko) 2013-01-24 2019-05-31 삼성전자주식회사 핵산 분석용 미세 유체 시스템
EP3473905B1 (en) 2013-01-25 2020-07-29 Bio-rad Laboratories, Inc. System and method for performing droplet inflation
US10381106B2 (en) 2013-01-28 2019-08-13 Hasso-Plattner-Institut Fuer Softwaresystemtechnik Gmbh Efficient genomic read alignment in an in-memory database
WO2014121241A1 (en) 2013-02-01 2014-08-07 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
JP2016514047A (ja) 2013-03-06 2016-05-19 プレジデント アンド フェローズ オブ ハーバード カレッジ 比較的単分散の液滴を形成するためのデバイスおよび方法
WO2014138711A1 (en) 2013-03-08 2014-09-12 Bio-Rad Laboratories, Inc. Compositions, methods and systems for polymerase chain reaction assays
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
US10612088B2 (en) 2013-03-14 2020-04-07 The Broad Institute, Inc. Massively multiplexed RNA sequencing
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
JP6587544B2 (ja) 2013-03-15 2019-10-09 シグニス バイオテク エセ.エレ.ウ.Sygnis Biotech S.L.U. 熱安定なTthPrimPolを用いた増幅及び配列決定方法
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
US11231419B2 (en) 2013-03-15 2022-01-25 Prognosys Biosciences, Inc. Methods for detecting peptide/MHC/TCR binding
WO2014145760A1 (en) 2013-03-15 2014-09-18 Bio-Rad Laboratories, Inc. Droplet generator with collection tube
CA2906818C (en) 2013-03-15 2022-08-16 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
EP2981349A4 (en) 2013-04-02 2016-11-16 Raindance Technologies Inc SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS
EP2994559B1 (en) 2013-05-09 2020-07-08 Bio-rad Laboratories, Inc. Magnetic immuno digital pcr assay
EP4321628A2 (en) 2013-05-23 2024-02-14 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
EP3587585B1 (en) 2013-06-12 2021-03-24 The General Hospital Corporation Methods, kits, and systems for multiplexed detection of target molecules and uses thereof
DK3011031T3 (da) 2013-06-17 2020-12-21 Broad Inst Inc Fremføring og anvendelse af crispr-cas-systemerne, vektorer og sammensætninger til levermålretning og -terapi
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
CA2915499A1 (en) 2013-06-27 2014-12-31 10X Genomics, Inc. Compositions and methods for sample processing
EP3019618B1 (en) 2013-07-12 2018-10-31 University of South Alabama Minimal piggybac vectors for genome integration
CN103394410B (zh) 2013-07-25 2016-04-20 博奥生物集团有限公司 一种位置可调节的智能磁力架
GB2516684A (en) 2013-07-30 2015-02-04 Sphere Fluidics Ltd Microfluidic devices and systems
KR102402446B1 (ko) 2013-08-28 2022-05-30 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CA2959978A1 (en) 2013-09-24 2015-04-02 The Regents Of The University Of California Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
EP3055431B1 (en) 2013-10-09 2020-06-24 Stc.Unm Synthetic long read dna sequencing
WO2015065924A2 (en) 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US20140315755A1 (en) 2013-12-26 2014-10-23 Tao Chen Genome-wide Antisense Oligonucleotide and RNAi
AU2014373757B2 (en) 2013-12-30 2019-12-12 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
KR101464100B1 (ko) 2014-01-29 2014-11-21 성균관대학교산학협력단 생체 적용 가능한 리포솜-핵산형광 나노 융합체, 이의 응용, 및 이의 제조방법
EP3102691B1 (en) 2014-02-03 2019-09-11 Thermo Fisher Scientific Baltics UAB Method for controlled dna fragmentation
EP2986761B1 (en) 2014-02-13 2018-08-15 Bio-rad Laboratories, Inc. Chromosome conformation capture in droplet partitions
CA2940669C (en) 2014-02-27 2023-10-03 Igenomx International Genomics Corporation Methods for analysis of somatic mobile elements, and uses thereof
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN113005108A (zh) 2014-04-15 2021-06-22 伊鲁米那股份有限公司 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
LT3456846T (lt) 2014-04-21 2022-09-12 President And Fellows Of Harvard College Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US10526601B2 (en) 2014-05-23 2020-01-07 Digenomix Corporation Haploidome determination by digitized transposons
EP3628684A1 (en) 2014-06-06 2020-04-01 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
WO2015191877A1 (en) 2014-06-11 2015-12-17 Life Technologies Corporation Systems and methods for substrate enrichment
US10704080B2 (en) 2014-06-11 2020-07-07 Samplix Aps Nucleotide sequence exclusion enrichment by droplet sorting (NEEDLS)
EP3155426B1 (en) 2014-06-13 2023-07-19 Immudex ApS General detection and isolation of specific cells by binding of labeled molecules
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
CN114214314A (zh) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 数字式pcr条码化
EP3161162A4 (en) 2014-06-26 2018-01-10 10X Genomics, Inc. Analysis of nucleic acid sequences
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2015200871A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods and compositions for sample analysis
CN113249435A (zh) 2014-06-26 2021-08-13 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
WO2015200891A1 (en) 2014-06-26 2015-12-30 10X Technologies, Inc. Processes and systems for nucleic acid sequence assembly
EP3940703A1 (en) 2014-07-18 2022-01-19 Illumina, Inc. Non-invasive prenatal diagnosis of fetal genetic conditions using cellular dna and cell free dna
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
CN105335116B (zh) 2014-07-30 2018-11-09 联想(北京)有限公司 一种显示控制方法及电子设备
WO2016033251A2 (en) 2014-08-26 2016-03-03 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CA2997906A1 (en) 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
MX2017003382A (es) 2014-09-15 2017-11-20 Abvitro Llc Secuenciacion de bibliotecas de nucleotidos de alto rendimiento.
WO2016061517A2 (en) 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition
BR112017008877A2 (pt) * 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US20180320241A1 (en) 2014-12-19 2018-11-08 Roche Sequencing Solutions, Inc. Methods for identifying multiple epitopes in selected sub-populations of cells
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN107209814B (zh) 2015-01-13 2021-10-15 10X基因组学有限公司 用于使结构变异和相位信息可视化的系统和方法
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CN107530654A (zh) 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
MX2017010142A (es) 2015-02-09 2017-12-11 10X Genomics Inc Sistemas y metodos para determinar variacion estructural y ajuste de fases con datos de recuperacion de variantes.
KR20170135834A (ko) 2015-02-10 2017-12-08 일루미나, 인코포레이티드 세포 성분을 분석하기 위한 방법 및 조성물
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016145409A1 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Genotype and phenotype coupling
WO2016149661A1 (en) 2015-03-18 2016-09-22 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
WO2016160908A1 (en) 2015-03-30 2016-10-06 Verily Life Sciences Llc Methods for combining single cell profiling with combinatorial nanoparticle conjugate library screening
JP2018511341A (ja) 2015-04-17 2018-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子配列決定および他の適用のためのバーコード化システムおよび方法
CN107250447B (zh) 2015-04-20 2020-05-05 深圳华大生命科学研究院 一种长片段dna文库构建方法
EP3708256B1 (en) 2015-04-22 2022-11-30 Stilla Technologies Contact-less priming method for loading a solution in a microfluidic device and associated system
US20160314242A1 (en) 2015-04-23 2016-10-27 10X Genomics, Inc. Sample indexing methods and compositions for sequencing applications
WO2016187256A2 (en) 2015-05-18 2016-11-24 10X Genomics, Inc. Mobile solid phase compositions for use in biochemical reactions and analyses
WO2016187179A1 (en) 2015-05-18 2016-11-24 10X Genomics, Inc. Stabilized reducing agents and methods using same
WO2016191380A1 (en) 2015-05-26 2016-12-01 Pacific Biosciences Of California, Inc. De novo diploid genome assembly and haplotype sequence reconstruction
WO2016187717A1 (en) 2015-05-26 2016-12-01 Exerkine Corporation Exosomes useful for genome editing
WO2016191618A1 (en) 2015-05-27 2016-12-01 Jianbiao Zheng Methods of inserting molecular barcodes
AU2016281766B2 (en) 2015-06-24 2019-05-09 Oxford BioDynamics PLC Epigenetic chromosome interactions
MX2018000729A (es) 2015-07-17 2018-09-06 Harvard College Métodos para amplificar las secuencias de ácido nucleico.
US10934636B2 (en) 2015-08-12 2021-03-02 CeMM—FORSCHUNGSZENTRUM FÜR MOLEKULARE MEDIZIN GmbH Methods for studying nucleic acids
US11479805B2 (en) 2015-08-21 2022-10-25 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
AU2016326737B2 (en) 2015-09-24 2023-01-12 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
US10900031B2 (en) 2015-10-19 2021-01-26 Zhejiang Annoroad Bio-Technology Co. Ltd. Method for constructing high-resolution single cell Hi-C library with a lot of information
CN108368522A (zh) 2015-10-20 2018-08-03 10X 基因组学有限公司 用于高通量单细胞遗传操作的方法和系统
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
CA3004310A1 (en) 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
SG11201803983UA (en) 2015-11-19 2018-06-28 10X Genomics Inc Transformable tagging compositions, methods, and processes incorporating same
EP3384048B1 (en) 2015-12-04 2021-03-24 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
WO2017117358A1 (en) 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Digital protein quantification
US20170260584A1 (en) 2016-02-11 2017-09-14 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
US20190078150A1 (en) 2016-03-01 2019-03-14 Universal Sequencing Technology Corporation Methods and Kits for Tracking Nucleic Acid Target Origin for Nucleic Acid Sequencing
US11680253B2 (en) 2016-03-10 2023-06-20 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
WO2017180420A1 (en) 2016-04-11 2017-10-19 Board Of Regents, The University Of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018031631A1 (en) 2016-08-10 2018-02-15 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic dna fragments
WO2018039338A1 (en) 2016-08-23 2018-03-01 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
WO2018044831A1 (en) 2016-08-30 2018-03-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
WO2018039969A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of whole genome digital amplification
US20180080021A1 (en) 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP4026905B1 (en) 2016-10-19 2024-04-17 10X Genomics, Inc. Methods for barcoding nucleic acid molecules from individual cells or cell populations
DK3553180T3 (da) 2016-12-07 2022-07-25 Mgi Tech Co Ltd Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf
EP3555290B1 (en) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
EP3565904A1 (en) 2016-12-29 2019-11-13 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
WO2018129368A2 (en) 2017-01-06 2018-07-12 Editas Medicine, Inc. Methods of assessing nuclease cleavage
EP3568508A4 (en) 2017-01-12 2020-12-16 Massachusetts Institute Of Technology METHODS FOR T-LYMPHOCYTE RECEPTOR AND B-LYMPHOCYTE RECEPTOR ANALYSIS
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US10347365B2 (en) 2017-02-08 2019-07-09 10X Genomics, Inc. Systems and methods for visualizing a pattern in a dataset
GB201704402D0 (en) 2017-03-20 2017-05-03 Blacktrace Holdings Ltd Single cell DNA sequencing
CN110462058A (zh) 2017-03-24 2019-11-15 新加坡国立大学 用于多重检测分子的方法
CA3059370C (en) 2017-04-12 2022-05-10 Karius, Inc. Methods for concurrent analysis of dna and rna in mixed samples
WO2018191701A1 (en) 2017-04-14 2018-10-18 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4215616A1 (en) 2017-05-18 2023-07-26 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
SG11201912824YA (en) 2017-06-20 2020-01-30 10X Genomics Inc Methods and systems for improved droplet stabilization
EP3642358A1 (en) 2017-06-21 2020-04-29 Bluedot LLC Systems and methods for identification of nucleic acids in a sample
CN108336542B (zh) 2017-06-23 2020-02-21 番禺得意精密电子工业有限公司 电连接器
CN111094585A (zh) 2017-08-01 2020-05-01 伊鲁米纳公司 用于核苷酸测序的水凝胶珠
US9946577B1 (en) 2017-08-14 2018-04-17 10X Genomics, Inc. Systems and methods for distributed resource management
US10357771B2 (en) 2017-08-22 2019-07-23 10X Genomics, Inc. Method of producing emulsions
CN111386134B (zh) 2017-10-04 2023-11-03 10X基因组学有限公司 使用改进的聚合物形成珠的组合物、方法和系统
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US20190127731A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods for preparing nucleic acid molecules
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084328A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS FOR PREPARING NUCLEIC ACID MOLECULES
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
WO2019148042A1 (en) 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
CN112004920A (zh) 2018-02-05 2020-11-27 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
SG11202008080RA (en) 2018-02-22 2020-09-29 10X Genomics Inc Ligation mediated analysis of nucleic acids
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019169347A1 (en) 2018-03-02 2019-09-06 10X Genomics, Inc. Systems and apparatus for holding plates
WO2019191321A1 (en) 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US20190345636A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20190352717A1 (en) 2018-05-18 2019-11-21 10X Genomics, Inc. Targeted non-invasive prenatal testing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US11574706B2 (en) 2018-06-28 2023-02-07 10X Genomics, Inc. Systems and methods for visualization of single-cell resolution characteristics
US20200033366A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112703252A (zh) 2018-08-03 2021-04-23 10X基因组学有限公司 用于最小化条形码交换的方法和系统
US20200056223A1 (en) 2018-08-20 2020-02-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020142779A1 (en) 2019-01-06 2020-07-09 10X Genomics, Inc. Methods and systems for enrichment of barcodes

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