SG11201803983UA - Transformable tagging compositions, methods, and processes incorporating same - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property ,- Organization International Bureau (43) International Publication Date ..... ..orr esol 26 May 2017(26.05.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111311111111111111111 2017/087910 Publication Number Al (51) International Patent Classification: CA 94114 (US). MIKKELSEN, Tarjei Sigurd; 4700 C12Q 1/68 (2006.01) C40B 40/06 (2006.01) Tassajara Road, Apt. 3412, Dublin, CA 94568 (US). C12P 19/34 (2006.01) C12N 15/10 (2006.01) HINDSON, Benjamin; 1789 Terra Court, Pleasanton, CA C12P 19/30 (2006.01) 94566 (US). (21) International Application Number: (74) Agents: LAM, Amy et al.; Wilson Sonsini Goodrich & PCT/US2016/062955 Rosati, 650 Page Mill Road, Palo Alto, CA 94304-1050 (US). (22) International Filing Date: 18 November 2016 (18.11.2016) (81) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (26) Publication Language: English BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (30) Priority Data: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 62/257,438 19 November 2015 (19.11.2015) US KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (71) Applicant: 10X GENOMICS, INC. [US/US]; 7068 Koll MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, Center Parkway, Suite 401, Pleasanton, CA 94566 (US). OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (72) Inventors: RYVKIN, Paul; 760 N 7th Street, Apt. 3210, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, San Jose, CA 95112 (US). UNDERWOOD, Jason; 10021 ZW. 40th Avenue NE, Seattle, WA 98125 (US). SCHNALL- LEVIN, Michael; 3945 20th Street, Unit 4, San Francisco, [Continued on next page] (54) Title: TRANSFORMABLE TAGGING COMPOSITIONS, METHODS, AND PROCESSES INCORPORATING SAME (57) : The present disclosure provides methods, systems 100 and compositions that provide transformable tagging moieties for 102 use in analytical operations, and particularly in analysis biological systems, such as in the analysis of expression in cell based gene 17 1 72 1—zzzzzzzzzz—t systems. N \ \ N_ + 106 100 120 ZZZZZZZZZZ < —<— N 11 .4t 122 106' I I I C::: , 11 Cr\ GC IN 0 Figure 1 IN 1 - 1 0 ei O WO 2017/087910 Al MIONIMOREIDEDINIEHOIRMICIMOHOMEDIS (84) Designated States (unless otherwise indicated, for every Published: kind of regional protection available): ARIPO (BW, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, GH, SZ, RU, with international search report (Art 21(3)) — before the expiration of the time limit for amending the TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, DE, LT, SE, GA, claims and to be republished in the event amendments (Rule 48.2(h)) of receipt of GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).