ATE537254T1 - Mutanter transposonvektor und verwendung davon - Google Patents
Mutanter transposonvektor und verwendung davonInfo
- Publication number
- ATE537254T1 ATE537254T1 AT06780756T AT06780756T ATE537254T1 AT E537254 T1 ATE537254 T1 AT E537254T1 AT 06780756 T AT06780756 T AT 06780756T AT 06780756 T AT06780756 T AT 06780756T AT E537254 T1 ATE537254 T1 AT E537254T1
- Authority
- AT
- Austria
- Prior art keywords
- cell
- gene
- transposon
- vector
- introducing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/30—Bird
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005196398 | 2005-07-05 | ||
JP2005196957 | 2005-07-06 | ||
PCT/JP2006/313302 WO2007004642A1 (ja) | 2005-07-05 | 2006-07-04 | 改変トランスポゾンベクター及びその利用方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE537254T1 true ATE537254T1 (de) | 2011-12-15 |
Family
ID=37604503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06780756T ATE537254T1 (de) | 2005-07-05 | 2006-07-04 | Mutanter transposonvektor und verwendung davon |
Country Status (5)
Country | Link |
---|---|
US (1) | US9175295B2 (de) |
EP (2) | EP1921140B1 (de) |
JP (1) | JP4927730B2 (de) |
AT (1) | ATE537254T1 (de) |
WO (1) | WO2007004642A1 (de) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102803484A (zh) * | 2009-06-11 | 2012-11-28 | 大学共同利用机关法人情报·系统研究机构 | 生产蛋白质的方法 |
US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9388465B2 (en) | 2013-02-08 | 2016-07-12 | 10X Genomics, Inc. | Polynucleotide barcode generation |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CN114891871A (zh) | 2012-08-14 | 2022-08-12 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
EP3567116A1 (de) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Verfahren und systeme zur verarbeitung von polynukleotiden |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP4321628A2 (de) | 2013-05-23 | 2024-02-14 | The Board of Trustees of the Leland Stanford Junior University | Transposition in natives chromatin für persönliche epigenomik |
US9694361B2 (en) | 2014-04-10 | 2017-07-04 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
CN113249435A (zh) | 2014-06-26 | 2021-08-13 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
AU2016207023B2 (en) | 2015-01-12 | 2019-12-05 | 10X Genomics, Inc. | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
CN115651972A (zh) | 2015-02-24 | 2023-01-31 | 10X 基因组学有限公司 | 用于靶向核酸序列覆盖的方法 |
EP3384048B1 (de) | 2015-12-04 | 2021-03-24 | 10X Genomics, Inc. | Verfahren und zusammensetzungen zur nukleinsäureanalyse |
US11674145B2 (en) * | 2016-01-25 | 2023-06-13 | The Regents Of The University Of California | Pathway integration and expression in host cells |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
WO2018112415A1 (en) | 2016-12-16 | 2018-06-21 | B-Mogen Biotechnologies, Inc. | ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
CN109526228B (zh) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
EP3775271A1 (de) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung |
CA3104288A1 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2824434B2 (ja) | 1989-11-28 | 1998-11-11 | 財団法人化学及血清療法研究所 | 新規発現ベクター |
AU3460195A (en) | 1994-08-24 | 1996-03-14 | Meiji Milk Products Co., Ltd. | Method of culturing avian cells and cell line obtained thereby |
JPH08165249A (ja) | 1994-12-14 | 1996-06-25 | Snow Brand Milk Prod Co Ltd | 骨強化作用を有する組成物 |
FR2737731B1 (fr) * | 1995-08-07 | 1997-10-10 | Pasteur Institut | Sequence de retroelements naturels ou synthetiques ayant pour fonction de permettre l'insertion de sequences nucleotidiques dans une cellule eucaryote |
US6140129A (en) * | 1997-09-17 | 2000-10-31 | Wisconsin Alumni Research Foundation | Chromosomal targeting in bacteria using FLP recombinase |
WO2000011155A1 (en) * | 1998-08-19 | 2000-03-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for genomic modification |
US20050130147A1 (en) * | 2001-10-09 | 2005-06-16 | Cpoyrat Pty. Ltd. | Methods of preparing a targeting vector and uses thereof |
KR100459870B1 (ko) * | 2002-02-22 | 2004-12-04 | 한국과학기술원 | 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법 |
AU2003231048A1 (en) * | 2002-04-22 | 2003-11-03 | Regents Of The University Of Minnesota | Transposon system and methods of use |
GB2403475B (en) * | 2003-07-01 | 2008-02-06 | Oxitec Ltd | Stable integrands |
-
2006
- 2006-07-04 AT AT06780756T patent/ATE537254T1/de active
- 2006-07-04 WO PCT/JP2006/313302 patent/WO2007004642A1/ja active Application Filing
- 2006-07-04 US US11/994,982 patent/US9175295B2/en not_active Expired - Fee Related
- 2006-07-04 EP EP06780756A patent/EP1921140B1/de not_active Not-in-force
- 2006-07-04 JP JP2007524072A patent/JP4927730B2/ja not_active Expired - Fee Related
- 2006-07-04 EP EP11175863.7A patent/EP2392657B1/de not_active Not-in-force
Also Published As
Publication number | Publication date |
---|---|
EP1921140A4 (de) | 2009-05-06 |
US20100281551A1 (en) | 2010-11-04 |
JPWO2007004642A1 (ja) | 2009-01-29 |
EP1921140B1 (de) | 2011-12-14 |
EP2392657B1 (de) | 2013-09-25 |
US9175295B2 (en) | 2015-11-03 |
EP1921140A1 (de) | 2008-05-14 |
EP2392657A1 (de) | 2011-12-07 |
WO2007004642A1 (ja) | 2007-01-11 |
JP4927730B2 (ja) | 2012-05-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE537254T1 (de) | Mutanter transposonvektor und verwendung davon | |
CY1124458T1 (el) | Διαγονιδιακος ποντικος που παραγει υβριδικα αντισωματα που περιεχουν ανθρωπινες μεταβλητες περιοχες και σταθερες περιοχες ποντικου | |
Veeramah et al. | The impact of whole-genome sequencing on the reconstruction of human population history | |
Latreille et al. | Optical mapping as a routine tool for bacterial genome sequence finishing | |
US7148054B2 (en) | Evolution of whole cells and organisms by recursive sequence recombination | |
Palumbi | Speciation and the evolution of gamete recognition genes: pattern and process | |
Kamath et al. | Genome-wide RNAi screening in Caenorhabditis elegans | |
US8377681B2 (en) | Evolution of whole cells and organisms by recursive sequence recombination | |
Zhang et al. | Synthetic genomes | |
Simeonov et al. | CRISPR-based tools in immunity | |
EP1391511A4 (de) | Künstliches menschliches chromosom mit dem gen für die lambda-leichte kette menschlicher antikörper | |
CN107760715A (zh) | 一种精准高效的转基因载体及其构建方法和应用 | |
JPWO2016104716A1 (ja) | 遺伝子のノックアウト方法 | |
WO2004024871A3 (en) | Antibodies and methods for generating genetically altered antibodies with high affinity | |
ATE522626T1 (de) | Verfahren und nukleotid-sequenzen, die bevorzugt die expression eines mutierten huntingtin-genes unterdrücken | |
CN109997192A (zh) | 用于基于规则的基因组设计的方法 | |
WO2004022738A8 (en) | Methods and compositions for the generation of humanized mice | |
Moffat | Triplex DNA finally comes of age: more than three decades after it was first synthesized, triple-stranded DNA is showing promise as a tool for cleaving genomes precisely and blocking gene transcription | |
US9562265B2 (en) | Primers and methods for determining RhD zygosity | |
Horii et al. | Generation of Floxed Mice by Sequential Electroporation | |
WO2004013333A3 (de) | Revertierung der negativ-selektiven wirkung von negativen markerproteinen als selektionsverfahren | |
AR041953A1 (es) | Secuencias de adn a partir de la region genomica tcd de photorhabdus luminiscens | |
Williams et al. | The math of making mutant mice | |
WO2004005474A3 (en) | A robust, inducible cardiac preferred expression system for transgenesis | |
Ohtsuka et al. | Construction of mouse 129/Ola BAC library for targeting experiments using E14 embryonic stem cells |