BR112015019159A2 - geração de código de barras de polinucleotídeos - Google Patents

geração de código de barras de polinucleotídeos

Info

Publication number
BR112015019159A2
BR112015019159A2 BR112015019159A BR112015019159A BR112015019159A2 BR 112015019159 A2 BR112015019159 A2 BR 112015019159A2 BR 112015019159 A BR112015019159 A BR 112015019159A BR 112015019159 A BR112015019159 A BR 112015019159A BR 112015019159 A2 BR112015019159 A2 BR 112015019159A2
Authority
BR
Brazil
Prior art keywords
polynucleotide
barcode generation
polynucleotides
polynucleotide barcode
processing
Prior art date
Application number
BR112015019159A
Other languages
English (en)
Inventor
Hindson Benjamin
NESS Kevin
Schnall-Levin Michael
Jarosz Mirna
Hardenbol Paul
Saxonov Serge
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51297676&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112015019159(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of BR112015019159A2 publication Critical patent/BR112015019159A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification

Abstract

resumo “geração de código de barras de polinucleotídeos” a presente invenção proporciona composições, métodos, sistemas e dispositivos para o processamento de polinucleotídeo. tal processamento de polinucleotídeo pode ser útil para uma variedade de aplicações, incluindo sequenciamento de polinucleotídeo. em alguns casos, esta invenção proporciona métodos para a geração de bibliotecas de código de barras de polinucleotídeo e para a fixação de tais polinucleotídeos à polinucleotídeos alvo.
BR112015019159A 2013-02-08 2014-02-07 geração de código de barras de polinucleotídeos BR112015019159A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361762435P 2013-02-08 2013-02-08
US201361800223P 2013-03-15 2013-03-15
US201361840403P 2013-06-27 2013-06-27
US201361844804P 2013-07-10 2013-07-10
PCT/US2014/015427 WO2014124338A1 (en) 2013-02-08 2014-02-07 Polynucleotide barcode generation

Publications (1)

Publication Number Publication Date
BR112015019159A2 true BR112015019159A2 (pt) 2017-07-18

Family

ID=51297676

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015019159A BR112015019159A2 (pt) 2013-02-08 2014-02-07 geração de código de barras de polinucleotídeos

Country Status (10)

Country Link
US (8) US9388465B2 (pt)
EP (4) EP2954065B1 (pt)
JP (4) JP2016511243A (pt)
KR (3) KR102190198B1 (pt)
CN (2) CN108753766A (pt)
AU (1) AU2014214682B2 (pt)
BR (1) BR112015019159A2 (pt)
CA (2) CA2900481A1 (pt)
DK (1) DK2954065T3 (pt)
WO (2) WO2014124338A1 (pt)

Families Citing this family (232)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
KR101793744B1 (ko) 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 유동 포커싱 미세유동 장치의 규모 확장
KR20120089661A (ko) 2009-09-02 2012-08-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 분출 및 다른 기술을 사용하여 생성되는 다중 에멀션
EP3842150A1 (en) 2009-10-27 2021-06-30 President and Fellows of Harvard College Droplet creation techniques
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2652155B1 (en) 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
KR20140034242A (ko) 2011-05-23 2014-03-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 다중 에멀젼을 포함하는 에멀젼의 제어
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
EP3919617A1 (en) 2013-03-13 2021-12-08 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015054292A1 (en) 2013-10-07 2015-04-16 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
DE202015009494U1 (de) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidische Vorrichtungen und Systeme zur Einkapselung und Partitionierung von Reagenzien, und deren Anwendungen
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
US11155809B2 (en) * 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
US10017759B2 (en) * 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
EP4105337A1 (en) 2014-09-09 2022-12-21 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
WO2016040901A1 (en) * 2014-09-12 2016-03-17 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acids
AU2015331739B2 (en) * 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US10060913B2 (en) 2016-09-19 2018-08-28 Massachusetts Institute Of Technology Systems including janus droplets capable of binding an analyte and changing orientation to provide a detectable change
WO2016070016A1 (en) 2014-10-31 2016-05-06 Massachusetts Institute Of Technology Compositions and methods for forming emulsions
US11119098B2 (en) 2014-10-31 2021-09-14 Massachusetts Institute Of Technology Systems including Janus droplets
US10005058B2 (en) * 2014-10-31 2018-06-26 Massachusetts Institute Of Technology Compositions and methods for arranging colloid phases
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
SG11201705425SA (en) 2015-01-13 2017-08-30 10X Genomics Inc Systems and methods for visualizing structural variation and phasing information
MX2017010142A (es) 2015-02-09 2017-12-11 10X Genomics Inc Sistemas y metodos para determinar variacion estructural y ajuste de fases con datos de recuperacion de variantes.
PT3256604T (pt) * 2015-02-10 2020-05-18 Illumina Inc Métodos e composições para analisar componentes celulares
JP2018509178A (ja) * 2015-02-13 2018-04-05 アジット パテール，アビジット 核酸の高度並列型および正確な測定方法
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
EP3262407B1 (en) * 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2016145416A2 (en) * 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
CN107614096A (zh) 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
EP3271849B1 (en) 2015-03-17 2021-09-08 Bio-Rad Laboratories, Inc. Detection of genome editing
US20180073073A1 (en) * 2015-03-18 2018-03-15 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
EP3835431B1 (en) 2015-03-30 2022-11-02 Becton, Dickinson and Company Methods for combinatorial barcoding
EP3530752B1 (en) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US11661597B2 (en) * 2015-04-15 2023-05-30 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
CA3184363C (en) 2015-04-21 2024-01-02 Isolation Bio Inc. High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
ES2826880T3 (es) 2015-05-11 2021-05-19 Illumina Inc Plataforma para el descubrimiento y análisis de agentes terapéuticos
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
US9771575B2 (en) 2015-06-19 2017-09-26 Agilent Technologies, Inc. Methods for on-array fragmentation and barcoding of DNA samples
WO2017015099A1 (en) 2015-07-17 2017-01-26 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
CN108289797B (zh) 2015-10-13 2022-01-28 哈佛学院院长及董事 用于制备和使用凝胶微球的系统和方法
WO2017070056A1 (en) 2015-10-20 2017-04-27 10X Genomics, Inc. Methods and systems for high throughput single cell genetic manipulation
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
WO2017075293A1 (en) * 2015-10-28 2017-05-04 Silicon Valley Scientific, Inc. Method and apparatus for encoding cellular spatial position information
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
US20170145476A1 (en) * 2015-11-19 2017-05-25 10X Genomics, Inc. Transformable tagging compositions, methods, and processes incorporating same
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
CN117512080A (zh) * 2015-12-07 2024-02-06 生物-拉德实验室公司 在分配中使用微粒的多路技术
CN105567562B (zh) * 2015-12-30 2017-10-03 中国科学院合肥物质科学研究院 用于单细胞超声波基因导入的微流体系统及其导入方法
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
US10633648B2 (en) 2016-02-12 2020-04-28 University Of Washington Combinatorial photo-controlled spatial sequencing and labeling
US20170283864A1 (en) 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
WO2017173328A1 (en) * 2016-04-01 2017-10-05 Baylor College Of Medicine Methods of whole transcriptome amplification
JP2019515674A (ja) * 2016-04-19 2019-06-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法
AU2017261189B2 (en) 2016-05-02 2023-02-09 Becton, Dickinson And Company Accurate molecular barcoding
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017204294A1 (ja) * 2016-05-25 2017-11-30 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2018027048A1 (en) 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
AU2017311306A1 (en) * 2016-08-10 2019-03-14 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic DNA fragments
US10688494B2 (en) 2016-08-23 2020-06-23 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
ES2870639T3 (es) 2016-10-24 2021-10-27 Geneinfosec Inc Ocultación de información presente en los ácidos nucleicos
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
JP7228510B2 (ja) 2016-11-08 2023-02-24 ベクトン・ディキンソン・アンド・カンパニー 細胞標識分類の方法
US20200190507A1 (en) * 2016-11-10 2020-06-18 The Scripps Research Institute Encoded Solid Phase Compound Library with Polynucleotide Based Barcoding
WO2018118971A1 (en) 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
EP3574112B1 (en) 2017-01-27 2023-01-11 Roche Diagnostics GmbH Barcoded dna for long range sequencing
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3391958B1 (en) * 2017-04-19 2020-08-12 The Procter & Gamble Company Method of making surface-coated water-absorbing polymer particles in a microfluidic device
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US20180320173A1 (en) 2017-05-05 2018-11-08 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10844372B2 (en) * 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
CN110799679A (zh) 2017-06-20 2020-02-14 10X基因组学有限公司 用于改善液滴稳定的方法和系统
CA3067048C (en) * 2017-08-01 2024-03-05 Illumina, Inc. Hydrogel beads for nucleotide sequencing
WO2019032760A1 (en) 2017-08-10 2019-02-14 Rootpath Genomics, Inc. IMPROVED METHOD OF ANALYZING NUCLEIC ACID CONTENT FROM MULTIPLE BIOLOGICAL PARTICLES
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
WO2019040546A1 (en) * 2017-08-23 2019-02-28 Roche Sequencing Solutions, Inc ENZYME SCREENING METHODS
US10636761B2 (en) * 2017-08-29 2020-04-28 Electronics And Telecommunications Reearch Institute Method of fabricating a semiconductor package
US10748643B2 (en) 2017-08-31 2020-08-18 10X Genomics, Inc. Systems and methods for determining the integrity of test strings with respect to a ground truth string
CN109486923B (zh) * 2017-09-11 2022-02-18 广州永诺生物科技有限公司 多重扩增子测序用引物系统、其应用以及测序文库的构建方法
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US11402400B2 (en) 2017-10-13 2022-08-02 Hewlett-Packard Development Company, L.P. Partition liquid into samples
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
CN111295443B (zh) 2017-11-02 2024-04-16 生物辐射实验室股份有限公司 基于转座酶的基因组分析
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
JP7049103B2 (ja) * 2017-12-11 2022-04-06 株式会社日立製作所 単一細胞の網羅的３’末端遺伝子発現解析法
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
WO2019148042A1 (en) * 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
EP3746552B1 (en) 2018-01-31 2023-12-27 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
JP7372927B6 (ja) 2018-02-12 2023-11-27 ナノストリング テクノロジーズ，インコーポレイティド 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019191900A1 (en) * 2018-04-03 2019-10-10 Burning Rock Biotech Compositions and methods for preparing nucleic acid libraries
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
DE102018207098A1 (de) * 2018-05-08 2019-11-14 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Nanostruktur-Sequenzierung von Nukleotidsträngen
US11654404B2 (en) 2018-05-18 2023-05-23 Massachusetts Institute Of Technology Light emitting droplets and related methods
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) * 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
JP2022506546A (ja) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
CA3127279A1 (en) 2019-02-12 2020-08-20 Therycell Gmbh Reverse immunosuppression
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
WO2020207963A1 (en) 2019-04-12 2020-10-15 Miltenyi Biotec B.V. & Co. KG Conjugates having an enzymatically releasable detection moiety and a barcode moiety
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020222798A1 (en) 2019-04-30 2020-11-05 Hewlett-Packard Development Company, L.P. Microfluidic devices
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114667353A (zh) * 2019-08-19 2022-06-24 通用测序技术公司 用于核酸测序用于追踪核酸片段起源的方法和组合物
WO2021072306A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
JP2023502924A (ja) 2019-11-15 2023-01-26 ミルテニー バイオテック ベー．フェー． ウント コー． カー・ゲー シングルセルインデクシングのための色およびバーコード付きのビーズ
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
JP2023511278A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞遺伝子プロファイリングのための方法およびシステム
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
EP4090328A4 (en) 2020-01-13 2024-02-14 Fluent Biosciences Inc EMULSION-BASED ACTIVE SCREENING
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
EP3859012A1 (en) 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US20210270703A1 (en) 2020-02-28 2021-09-02 10X Genomics, Inc. Method for isolating nuclei and cells from tissues
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
EP4136227A1 (en) 2020-04-16 2023-02-22 10X Genomics, Inc. Compositions and methods for use with fixed samples
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
WO2021247568A1 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Spatial trancriptomics for antigen-receptors
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
CN116034166A (zh) 2020-06-25 2023-04-28 10X基因组学有限公司 Dna甲基化的空间分析
EP4176437A1 (en) 2020-07-02 2023-05-10 10X Genomics, Inc. Systems and methods for detection of low-abundance molecular barcodes from a sequencing library
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4182477A1 (en) * 2020-07-15 2023-05-24 Fluent Biosciences Inc. Tiered ligation oligos
CN115997126A (zh) 2020-09-07 2023-04-21 美天施生物科技有限两合公司 具有可酶促释放的检测部分和条形码部分的缀合物
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4301870A1 (en) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2023014303A2 (en) * 2021-08-05 2023-02-09 Agency For Science, Technology And Research A method of amplification of a nucleic acid
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
KR102578493B1 (ko) 2023-04-26 2023-09-14 대한민국 적재용 선반

Family Cites Families (778)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1007137A (en) 1908-06-08 1911-10-31 Theodore D Palmer Cloth-pressing machine.
US1004111A (en) 1908-10-17 1911-09-26 Westinghouse Air Brake Co Fluid-pressure brake.
US1003026A (en) 1911-04-12 1911-09-12 Bernard Bartmann Bobbin-holder.
US1005998A (en) 1911-07-10 1911-10-17 Arthur Munchausen Berry-carrier.
US1005372A (en) 1911-08-11 1911-10-10 Albert Gay Plow-fender.
US2797149A (en) 1953-01-08 1957-06-25 Technicon International Ltd Methods of and apparatus for analyzing liquids containing crystalloid and non-crystalloid constituents
US3047367A (en) 1959-12-01 1962-07-31 Technicon Instr Automatic analysis with fluid segmentation
US3479141A (en) 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
US4124638A (en) 1977-09-12 1978-11-07 Hansen John N Solubilizable polyacrylamide gels containing disulfide cross-linkages
US4253846A (en) 1979-11-21 1981-03-03 Technicon Instruments Corporation Method and apparatus for automated analysis of fluid samples
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
DE3230289A1 (de) 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen Herstellung von pharmazeutischen oder kosmetischen dispersionen
US4582802A (en) 1983-09-30 1986-04-15 The United States Of America As Represented By The Department Of Health And Human Services Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers
JPS60227826A (ja) 1984-04-27 1985-11-13 Sogo Yatsukou Kk ｐＨに応答するグラフトカプセル
KR890003947B1 (ko) * 1985-12-11 1989-10-13 가부시기가이샤 시마즈세이사구쇼 세포융합장치
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4872895A (en) 1986-12-11 1989-10-10 American Telephone And Telegraph Company, At&T Bell Laboratories Method for fabricating articles which include high silica glass bodies
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5185099A (en) 1988-04-20 1993-02-09 Institut National De Recherche Chimique Appliquee Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US6176962B1 (en) 1990-02-28 2001-01-23 Aclara Biosciences, Inc. Methods for fabricating enclosed microchannel structures
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
WO1992001814A2 (en) 1990-07-24 1992-02-06 F. Hoffmann-La-Roche Ag The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0594772B1 (en) 1991-07-04 1996-08-28 Immunodex K/S Water-soluble, polymer-based reagents and conjugates comprising moieties derived from divinyl sulfone
JPH06509473A (ja) 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
DK0604552T3 (da) 1991-09-18 1997-08-04 Affymax Tech Nv Fremgangsmåde til syntese af forskellige samlinger af oligomerer
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
WO1993019205A1 (en) 1992-03-19 1993-09-30 The Regents Of The University Of California Multiple tag labeling method for dna sequencing
WO1993022058A1 (en) 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Polynucleotide amplification analysis using a microfabricated device
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5840865A (en) 1992-09-14 1998-11-24 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
US5897783A (en) 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
US5569364A (en) 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
JPH08506664A (ja) 1993-02-01 1996-07-16 セック，リミテッド Ｄｎａ配列決定の方法および装置
AU6175594A (en) 1993-02-16 1994-09-14 Alliance Pharmaceutical Corporation Method of microemulsifying fluorinated oils
AU6637394A (en) 1993-04-19 1994-11-08 Medisorb Technologies International L.P. Long-acting treatment by slow-release delivery of antisense oligodeoxyribonucleotides from biodegradable microparticles
US5456986A (en) * 1993-06-30 1995-10-10 Carnegie Mellon University Magnetic metal or metal carbide nanoparticles and a process for forming same
ATE208658T1 (de) 1993-07-28 2001-11-15 Pe Corp Ny Vorrichtung und verfahren zur nukleinsäurevervielfältigung
US5874239A (en) 1993-07-30 1999-02-23 Affymax Technologies N.V. Biotinylation of proteins
WO1995006652A1 (en) 1993-08-30 1995-03-09 Promega Corporation Nucleic acid purification compositions and methods
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
US20030044777A1 (en) 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5558071A (en) 1994-03-07 1996-09-24 Combustion Electromagnetics, Inc. Ignition system power converter and controller
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
EP0775313B1 (en) 1994-05-11 2000-03-15 Genera Technologies Limited Method of capturing species from liquids and apparatus therefor
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
EP0812434B1 (en) 1995-03-01 2013-09-18 President and Fellows of Harvard College Microcontact printing on surfaces and derivative articles
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
ATE466109T1 (de) 1995-06-07 2010-05-15 Solexa Inc Verfahren zur verbesserung der effizienz der polynukleotidsequenzierung
EP0832287B1 (en) 1995-06-07 2007-10-10 Solexa, Inc Oligonucleotide tags for sorting and identification
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6866760B2 (en) 1998-08-27 2005-03-15 E Ink Corporation Electrophoretic medium and process for the production thereof
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
US5736332A (en) 1995-11-30 1998-04-07 Mandecki; Wlodek Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
US6051377A (en) 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
EP0832436A1 (en) 1996-04-15 1998-04-01 Dade Behring Inc. Apparatus and method for analysis
EP0912761A4 (en) 1996-05-29 2004-06-09 Cornell Res Foundation Inc DETERMINATION OF DIFFERENCES IN THE NUCLEIC ACID SEQUENCE BY MEANS OF A COMBINATION OF LIGASE DETERMINATION AND POLYMERASE CHAIN REACTION
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
AU4114397A (en) 1996-07-15 1998-02-09 Kemgas Limited Production of powders
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6379929B1 (en) 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US20020172965A1 (en) 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US20050042625A1 (en) 1997-01-15 2005-02-24 Xzillion Gmbh & Co. Mass label linked hybridisation probes
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
US20020034737A1 (en) 1997-03-04 2002-03-21 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US20030027203A1 (en) 1997-03-24 2003-02-06 Fields Robert E. Biomolecular processor
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
KR20010012175A (ko) 1997-05-02 2001-02-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 폴리뉴클레오티드의 2단계 혼성화 및 포획법
DE69823347T2 (de) 1997-05-16 2005-05-12 Alberta Research Council, Edmonton Mikrofluidisches system und verfahren zu dessen betrieb
EP0985142A4 (en) 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US20040241759A1 (en) 1997-06-16 2004-12-02 Eileen Tozer High throughput screening of libraries
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
GB9714716D0 (en) 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US20050037397A1 (en) 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6368871B1 (en) 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
WO1999009217A1 (en) 1997-08-15 1999-02-25 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
WO1999014368A2 (en) 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
US20020092767A1 (en) 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
EP1029244A4 (en) 1997-10-02 2003-07-23 Aclara Biosciences Inc CAPILLAR ASSAY WITH SEPARATION OF FREE AND TIED SPECIES
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
HUP0003986A3 (en) 1997-10-14 2001-04-28 Luminex Corp Austin Precision fluorescently dyed particles and methods of making and using same
JP2001521169A (ja) 1997-10-24 2001-11-06 ノースイースタン・ユニバーシティ 広範囲の採集と分析とで高スループットの調整分離のためのマルチチャンネルの微小規模システム
CA2313047A1 (en) 1997-12-04 1999-12-16 Nicholas Thomas Multiple assay method
WO1999033963A1 (en) 1997-12-31 1999-07-08 Chiron Corporation Metastatic cancer regulated gene
IL137706A0 (en) 1998-02-19 2001-10-31 Harvard College Mhc fusion proteins and conjugates
US6207384B1 (en) 1998-03-27 2001-03-27 The General Hospital Corporation Systematic identification of essential genes by in vitro transposon mutagenesis
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
AU3555599A (en) 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US5997636A (en) 1998-05-01 1999-12-07 Instrumentation Technology Associates, Inc. Method and apparatus for growing crystals
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
AU754952B2 (en) 1998-06-24 2002-11-28 Illumina, Inc. Decoding of array sensors with microspheres
US6586176B1 (en) 1998-08-07 2003-07-01 Cellay, Llc Gel microdrops in genetic analysis
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
BR9914554A (pt) 1998-10-13 2001-06-26 Biomicro Systems Inc Componentes de circuito fluido com base em dinâmica dos fluidos passiva
US6489096B1 (en) 1998-10-15 2002-12-03 Princeton University Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays
SE9803614L (sv) 1998-10-19 2000-04-20 Muhammed Mamoun Förfarande och anordning för framställning av nanopartiklar
WO2000026412A1 (en) 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US6569631B1 (en) 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US6635419B1 (en) 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
US20030027214A1 (en) 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
AU3372800A (en) 1999-02-23 2000-09-14 Caliper Technologies Corporation Manipulation of microparticles in microfluidic systems
US7615373B2 (en) 1999-02-25 2009-11-10 Virginia Commonwealth University Intellectual Property Foundation Electroprocessed collagen and tissue engineering
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
CA2370976C (en) 1999-04-20 2009-10-20 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6291243B1 (en) 1999-04-28 2001-09-18 The Board Of Trustees Of The Leland Stanford Jr. University P element derived vector and methods for its use
JP3815969B2 (ja) 1999-05-12 2006-08-30 アクララ バイオサイエンシーズ， インコーポレイテッド 微量流体デバイスにおける多重方式蛍光検出
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6846622B1 (en) 1999-05-26 2005-01-25 Oregon Health & Science University Tagged epitope protein transposable element
US20030124509A1 (en) 1999-06-03 2003-07-03 Kenis Paul J.A. Laminar flow patterning and articles made thereby
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
WO2001013086A2 (en) 1999-08-13 2001-02-22 Brandeis University Detection of nucleic acids
AU6788100A (en) 1999-08-20 2001-03-19 Luminex Corporation Liquid array technology
ATE414171T1 (de) 1999-08-27 2008-11-15 Matrix Technologies Corp Verfahren zur immobilisierung von oligonukleotiden auf festem trägermaterial
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
KR20020071853A (ko) 1999-10-13 2002-09-13 시그나츄어 바이오사이언스 인코포레이티드 테스트 샘플에서 분자 이벤트를 탐지 및 규명하는 시스템및 방법
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
WO2001030965A2 (en) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
JP4721603B2 (ja) 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US20010051348A1 (en) 2000-01-28 2001-12-13 Lee Chee Wee Novel ligands and methods for preparing same
CN1444646A (zh) 2000-02-23 2003-09-24 齐翁米克斯股份有限公司 具有凸起的样品表面的芯片
AU2001249071B2 (en) 2000-02-23 2005-09-08 Caliper Life Sciences, Inc. Multi-reservoir pressure control system
IL134830A0 (en) 2000-03-01 2001-05-20 Chay 13 Medical Res Group N V Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them
CA2402733A1 (en) 2000-03-14 2001-09-20 Burkhard Goke Effects of glucagon-like peptide-1 (7-36) on antro-pyloro-duodenal motility
CA2404008A1 (en) 2000-03-31 2001-10-11 Jurgen Sygusch Protein crystallization in microfluidic structures
AU2001250058A1 (en) 2000-04-06 2001-10-23 Caliper Technologies Corp. Methods and devices for achieving long incubation times in high-throughput systems
DK2206791T3 (en) 2000-04-10 2016-10-24 Taxon Biosciences Inc Methods of study and genetic analysis of populations
US6481453B1 (en) 2000-04-14 2002-11-19 Nanostream, Inc. Microfluidic branch metering systems and methods
US6800298B1 (en) 2000-05-11 2004-10-05 Clemson University Biological lubricant composition and method of applying lubricant composition
US20060008799A1 (en) 2000-05-22 2006-01-12 Hong Cai Rapid haplotyping by single molecule detection
WO2001090614A2 (en) 2000-05-24 2001-11-29 Micronics, Inc. Surface tension valves for microfluidic applications
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US6632606B1 (en) 2000-06-12 2003-10-14 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
JP4744778B2 (ja) 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド 特定のランダム粒子アレイを適用した複数の被検体分子の分析方法
WO2002011888A2 (en) 2000-08-07 2002-02-14 Nanostream, Inc. Fluidic mixer in microfluidic system
US6610499B1 (en) 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
EP1317569B1 (en) 2000-09-14 2009-11-18 Caliper Life Sciences, Inc. Microfluidic devices and methods for performing temperature mediated reactions
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
WO2002029106A2 (en) 2000-10-03 2002-04-11 California Institute Of Technology Microfluidic devices and methods of use
JP2005501217A (ja) 2000-10-10 2005-01-13 ディベルサ コーポレーション 生体活性または生体分子のハイスループットスクリーニングまたはキャピラリーに基づくスクリーニング
JP2002155305A (ja) 2000-11-14 2002-05-31 Akira Kawasaki 単分散粒子の製造装置及び単分散粒子の製造方法及びその製造方法で製造された単分散粒子
US6492154B2 (en) 2001-01-31 2002-12-10 Applera Corporation Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof
CA2332186A1 (en) 2001-02-08 2002-08-08 Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Ture And Agri-Food Canada Replicative in vivo gene targeting
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
DE60207831D1 (de) 2001-02-22 2006-01-12 Anika Therapeutics Inc Thiol-modifizierte hyaluronan-derivate
US7268167B2 (en) 2001-02-23 2007-09-11 Japan Science And Technology Agency Process for producing emulsion and microcapsules and apparatus therefor
US20150329617A1 (en) 2001-03-14 2015-11-19 Dynal Biotech Asa Novel MHC molecule constructs, and methods of employing these constructs for diagnosis and therapy, and uses of MHC molecules
US7211654B2 (en) 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
WO2002081934A2 (en) 2001-04-03 2002-10-17 Micronics, Inc. Pneumatic valve interface for use in microfluidic structures
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US20030027221A1 (en) * 2001-04-06 2003-02-06 Scott Melissa E. High-throughput screening assays by encapsulation
US7572642B2 (en) 2001-04-18 2009-08-11 Ambrigen, Llc Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns
JP4194934B2 (ja) 2001-05-26 2008-12-10 ワン セル システムズ インコーポレイテッド カプセル化細胞によるタンパク質の分泌法
US6880576B2 (en) 2001-06-07 2005-04-19 Nanostream, Inc. Microfluidic devices for methods development
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7682353B2 (en) 2001-06-29 2010-03-23 Coloplast A/S Catheter device
US7077152B2 (en) 2001-07-07 2006-07-18 Nanostream, Inc. Microfluidic metering systems and methods
US7799552B2 (en) 2001-07-20 2010-09-21 California Institute Of Technology Protein and nucleic acid expression systems
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
JP2005509872A (ja) 2001-10-19 2005-04-14 ウエスト バージニア ユニバーシティ リサーチ コーポレーション プロテオーム解析のための微小流体システム
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
US20030149307A1 (en) 2001-10-24 2003-08-07 Baxter International Inc. Process for the preparation of polyethylene glycol bis amine
US20040195728A1 (en) 2001-10-26 2004-10-07 Dennis Slomski System and method for injection molded micro-replication of micro-fluidic substrates
CA2466164A1 (en) 2001-10-30 2003-05-08 Nanomics Biosystems Pty, Ltd. Device and methods for directed synthesis of chemical libraries
US7262056B2 (en) 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
WO2003046208A2 (en) 2001-11-28 2003-06-05 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
US20030170698A1 (en) 2002-01-04 2003-09-11 Peter Gascoyne Droplet-based microfluidic oligonucleotide synthesis engine
US8110351B2 (en) 2002-01-16 2012-02-07 Invitrogen Dynal As Method for isolating nucleic acids and protein from a single sample
KR100459870B1 (ko) 2002-02-22 2004-12-04 한국과학기술원 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
AU2003226679A1 (en) * 2002-03-20 2003-09-29 Innovativebio.Biz Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP4855680B2 (ja) 2002-05-09 2012-01-18 ザ・ユニバーシティ・オブ・シカゴ 圧力駆動プラグによる輸送と反応のための装置および方法
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP1543157A4 (en) 2002-07-24 2006-11-15 Ptc Therapeutics Inc METHODS FOR IDENTIFYING SMALL MOLECULES MODULATING PREMATURE TRANSLATION TERMINATION AND DEGRADATION OF INDUCED MRNA BY NON-SENSE MUTATION
IL151660A0 (en) 2002-09-09 2003-04-10 Univ Ben Gurion Method for isolating and culturing unculturable microorganisms
ATE414177T1 (de) 2002-09-30 2008-11-15 Hoffmann La Roche Oligonukleotide zur genotypisierung des thymidylat-synthase gens
US20040081962A1 (en) 2002-10-23 2004-04-29 Caifu Chen Methods for synthesizing complementary DNA
US6979713B2 (en) 2002-11-25 2005-12-27 3M Innovative Properties Company Curable compositions and abrasive articles therefrom
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US20040248299A1 (en) 2002-12-27 2004-12-09 Sumedha Jayasena RNA interference
WO2004065617A2 (en) 2003-01-17 2004-08-05 The Trustees Of Boston University Haplotype analysis
ES2342665T3 (es) 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
WO2004070042A1 (en) 2003-02-10 2004-08-19 Max-Delbrück-Centrum Für Molekulare Medizin (Mdc) Transposon-based targeting system
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7316903B2 (en) 2003-03-28 2008-01-08 United States Of America As Represented By The Department Of Health And Human Services Detection of nucleic acid sequence variations using phase Mu transposase
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
JP2006522090A (ja) 2003-04-04 2006-09-28 ファイザー・プロダクツ・インク マイクロ流動化された水中油形エマルジョン及びワクチン組成物
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
EP3616781A1 (en) 2003-04-10 2020-03-04 President and Fellows of Harvard College Formation and control of fluidic species
EP1629286A1 (en) 2003-05-16 2006-03-01 Global Technologies (NZ) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
WO2004103565A2 (de) 2003-05-19 2004-12-02 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Vorrichtung und verfahren zur strukturierung von flüssigkeiten und zum zudosieren von reaktionsflüssigkeiten zu in separationsmedium eingebetteten flüssigkeitskompartimenten
WO2004105734A1 (en) 2003-05-28 2004-12-09 Valorisation Recherche, Societe En Commandite Method of preparing microcapsules
GB0313170D0 (en) 2003-06-09 2003-07-16 Qinetiq Ltd Method and apparatus for spore disruption and/or detection
CA2529285A1 (en) 2003-06-13 2004-12-29 The General Hospital Corporation Microfluidic systems for size based removal of red blood cells and platelets from blood
GB2403475B (en) 2003-07-01 2008-02-06 Oxitec Ltd Stable integrands
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
JP5183063B2 (ja) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
EP1658133A1 (en) 2003-08-27 2006-05-24 President And Fellows Of Harvard College Electronic control of fluidic species
JP4988345B2 (ja) 2003-09-04 2012-08-01 ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプレゼンティッド・バイ・ザ・デパートメント・オブ・ヴェテランズ・アフェアーズ 眼用ハイドロゲルナノコンポジット
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
US7354706B2 (en) 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
EP1672064A4 (en) 2003-09-22 2006-12-20 Riken EFFICIENT METHOD FOR GENERATING AN INVERTED REPEAT DNA STRUCTURE
WO2005069001A1 (ja) 2003-09-25 2005-07-28 Toyama Prefecture マイクロウェルアレイチップ及びその製造方法
EP1694869A2 (en) 2003-11-10 2006-08-30 Investigen, Inc. Methods of preparing nucleic acid for detection
WO2005049787A2 (en) 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
US20050136417A1 (en) 2003-12-19 2005-06-23 Affymetrix, Inc. Amplification of nucleic acids
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
CA2553833C (en) 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
US20050181379A1 (en) 2004-02-18 2005-08-18 Intel Corporation Method and device for isolating and positioning single nucleic acid molecules
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
WO2005082098A2 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
WO2005089921A1 (ja) 2004-03-23 2005-09-29 Japan Science And Technology Agency 微小液滴の生成方法及び装置
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2005099419A2 (en) 2004-04-13 2005-10-27 President And Fellows Of Harvard College Manipulation and/or detection of biological samples or other objects
WO2005111242A2 (en) 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
US20080268507A1 (en) 2004-05-25 2008-10-30 Airbus Deutschland Gmbh Recombinant Dna Nicking Endonuclease and Uses Thereof
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US7968085B2 (en) 2004-07-05 2011-06-28 Ascendis Pharma A/S Hydrogel formulations
CN1648671B (zh) * 2005-02-06 2012-09-26 成都夸常医学工业有限公司 多反应器分析芯片检测方法和分析芯片及检测装置
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
WO2006030993A1 (en) 2004-09-14 2006-03-23 Jin-Ho Choy Information code system using dna sequences
US7892731B2 (en) 2004-10-01 2011-02-22 Radix Biosolutions, Ltd. System and method for inhibiting the decryption of a nucleic acid probe sequence used for the detection of a specific nucleic acid
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2007001448A2 (en) 2004-11-04 2007-01-04 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
WO2006051552A2 (en) 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
US7329493B2 (en) 2004-12-22 2008-02-12 Asiagen Corporation One-tube nested PCR for detecting Mycobacterium tuberculosis
EP2418018B1 (en) 2004-12-23 2013-05-22 Abbott Point of Care Inc. Methods for the separation nucleic acids
WO2006078841A1 (en) 2005-01-21 2006-07-27 President And Fellows Of Harvard College Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles
WO2006081222A2 (en) 2005-01-25 2006-08-03 Compass Genetics, Llc. Isothermal dna amplification
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
CN101163800B (zh) 2005-02-18 2013-04-17 佳能美国生命科学公司 鉴定生物的基因组dna的装置和方法
EP1867702B1 (en) * 2005-02-21 2011-09-28 Kagoshima University Method for purifying biodiesel fuel
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
JP2008535644A (ja) 2005-03-04 2008-09-04 プレジデント・アンド・フエローズ・オブ・ハーバード・カレツジ 多重エマルジョンの形成のための方法および装置
JP2006289250A (ja) 2005-04-08 2006-10-26 Kao Corp マイクロミキサー及びそれを用いた流体混合方法
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
JP2006349060A (ja) 2005-06-16 2006-12-28 Ntn Corp ボールねじ
US8828209B2 (en) 2005-06-22 2014-09-09 The Research Foundation For The State University Of New York Massively parallel 2-dimensional capillary electrophoresis
US20070154903A1 (en) 2005-06-23 2007-07-05 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
JP4927730B2 (ja) 2005-07-05 2012-05-09 一般財団法人化学及血清療法研究所 改変トランスポゾンベクター及びその利用方法
US9206384B2 (en) 2011-12-03 2015-12-08 Emd Millipore Corporation Micro-incubation systems for microfluidic cell culture and methods
JP5051490B2 (ja) 2005-07-08 2012-10-17 独立行政法人産業技術総合研究所 マクロ生体材料を内包する無機マイクロカプセルおよびその製造方法
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
FR2888912B1 (fr) 2005-07-25 2007-08-24 Commissariat Energie Atomique Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
DE102005037401B4 (de) 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
WO2007024840A2 (en) 2005-08-22 2007-03-01 Critical Therapeutics, Inc. Method of quantitating nucleic acids by flow cytometry microparticle-based array
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
JP2007074967A (ja) 2005-09-13 2007-03-29 Canon Inc 識別子プローブ及びそれを用いた核酸増幅方法
WO2007084192A2 (en) 2005-09-16 2007-07-26 The Regents Of The University Of California A colorimetric bio-barcode amplification assay for analyte detection
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070111241A1 (en) 2005-10-14 2007-05-17 Nezih Cereb System and method for accessing, tracking, and editing sequence analysis and software to accomplish the same
US7709544B2 (en) 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
EP1954838B1 (en) * 2005-11-14 2014-02-26 Life Technologies Corporation Coded molecules for detecting target analytes
US20070134277A1 (en) 2005-12-09 2007-06-14 Children's Medical Center Corporation Pharmaceutical formulation for sulfur-containing drugs in liquid dosage forms
US20070141584A1 (en) 2005-12-20 2007-06-21 Roberts Douglas N Methods for assessment of native chromatin on microarrays
US7932037B2 (en) 2007-12-05 2011-04-26 Perkinelmer Health Sciences, Inc. DNA assays using amplicon probes on encoded particles
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
US7544473B2 (en) 2006-01-23 2009-06-09 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
JP2009524825A (ja) 2006-01-27 2009-07-02 プレジデント アンド フェロウズ オブ ハーバード カレッジ 流体ドロップレットの合体
EP3002338B1 (en) 2006-02-02 2019-05-08 The Board of Trustees of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603251D0 (en) 2006-02-17 2006-03-29 Isis Innovation DNA conformation
JP5180845B2 (ja) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド Ｄｎａアレイ上でのハイスループットゲノム配列決定
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
JP4921829B2 (ja) 2006-03-30 2012-04-25 株式会社東芝 微粒子の製造装置、乳化剤保持部、微粒子の製造方法および分子膜の製造方法
US20090181864A1 (en) 2006-03-31 2009-07-16 Nam Trung Nguyen Active control for droplet-based microfluidics
JP2009538123A (ja) 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US7811603B2 (en) 2006-05-09 2010-10-12 The Regents Of The University Of California Microfluidic device for forming monodisperse lipoplexes
US20080003142A1 (en) 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
CA2653095C (en) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
RU2321638C2 (ru) 2006-05-23 2008-04-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Способ изготовления многофункционального мультичипа, мультичип для последовательного или параллельного скрининга биополимеров, способ анализа биополимеров и набор для осуществления способа
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
FR2901717A1 (fr) 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
AU2007260676A1 (en) 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
WO2007149432A2 (en) 2006-06-19 2007-12-27 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
US20080076909A1 (en) 2006-06-30 2008-03-27 Applera Corporation Emulsion pcr and amplicon capture
EP1878501A1 (en) 2006-07-14 2008-01-16 Roche Diagnostics GmbH Instrument for heating and cooling
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
EP2064346B1 (en) 2006-09-06 2013-11-06 Canon U.S. Life Sciences, Inc. Chip and cartridge design configuration for performing micro-fluidic assays
EP2066699B1 (en) 2006-09-25 2020-02-12 Archer-Daniels-Midland Company Superabsorbent surface-treated carboxyalkylated polysaccharides and process for producing same
US7935518B2 (en) 2006-09-27 2011-05-03 Alessandra Luchini Smart hydrogel particles for biomarker harvesting
US20080166720A1 (en) 2006-10-06 2008-07-10 The Regents Of The University Of California Method and apparatus for rapid nucleic acid analysis
WO2008052138A2 (en) 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
EP2091647A2 (en) 2006-11-14 2009-08-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
CA2669728C (en) 2006-11-15 2017-04-11 Biospherex Llc Multitag sequencing and ecogenomics analysis
CN101583879A (zh) * 2006-11-20 2009-11-18 纳提维斯公司 利用低频信号转导体外系统或者哺乳动物系统的设备和方法
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
JP5320546B2 (ja) 2006-12-13 2013-10-23 国立大学法人名古屋大学 Ｔｏｌ１因子のトランスポザーゼ及びそれを用いたＤＮＡ導入システム
JP5622392B2 (ja) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション 大規模ｆｅｔアレイを用いた分析物測定のための方法および装置
US8338166B2 (en) * 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
JP2008167722A (ja) 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
US7844658B2 (en) 2007-01-22 2010-11-30 Comcast Cable Holdings, Llc System and method for providing an application to a device
US20080176768A1 (en) 2007-01-23 2008-07-24 Honeywell Honeywell International Hydrogel microarray with embedded metal nanoparticles
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20080228268A1 (en) 2007-03-15 2008-09-18 Uluru, Inc. Method of Formation of Viscous, Shape Conforming Gels and Their Uses as Medical Prosthesis
US7943330B2 (en) 2007-03-23 2011-05-17 Academia Sinica Tailored glycoproteomic methods for the sequencing, mapping and identification of cellular glycoproteins
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
CN101293191B (zh) 2007-04-25 2011-11-09 中国科学院过程工程研究所 一种琼脂糖凝胶微球的制备方法
WO2008135512A2 (en) 2007-05-02 2008-11-13 Jerzy Paszkowski Dna amplification method
US20080295909A1 (en) 2007-05-24 2008-12-04 Locascio Laurie E Microfluidic Device for Passive Sorting and Storage of Liquid Plugs Using Capillary Force
US20090105959A1 (en) 2007-06-01 2009-04-23 Braverman Michael S System and method for identification of individual samples from a multiplex mixture
CA2689427C (en) 2007-06-05 2015-12-29 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009005680A1 (en) 2007-06-29 2009-01-08 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US20130084243A1 (en) 2010-01-27 2013-04-04 Liliane Goetsch Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders
CA2696843A1 (en) 2007-08-15 2009-02-19 Opgen, Inc. Method, system and software arrangement for comparative analysis and phylogeny with whole-genome optical maps
US8563527B2 (en) 2007-08-20 2013-10-22 Pharmain Corporation Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
WO2009048532A2 (en) 2007-10-05 2009-04-16 President And Fellows Of Harvard College Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation
US20090099040A1 (en) 2007-10-15 2009-04-16 Sigma Aldrich Company Degenerate oligonucleotides and their uses
US20100086914A1 (en) 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
WO2009061372A1 (en) 2007-11-02 2009-05-14 President And Fellows Of Harvard College Systems and methods for creating multi-phase entities, including particles and/or fluids
US8334013B2 (en) 2007-11-02 2012-12-18 Stc.Unm Mesoporous metal oxide microspheres and method for forming same
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US20110008775A1 (en) 2007-12-10 2011-01-13 Xiaolian Gao Sequencing of nucleic acids
US7771944B2 (en) 2007-12-14 2010-08-10 The Board Of Trustees Of The University Of Illinois Methods for determining genetic haplotypes and DNA mapping
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
EP3699291A1 (en) 2008-01-17 2020-08-26 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
KR20090081260A (ko) 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
WO2009100382A1 (en) 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
JP5468271B2 (ja) 2008-02-08 2014-04-09 花王株式会社 微粒子分散液の製造方法
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
EP2253713B1 (en) 2008-03-11 2015-02-25 National Cancer Center Method for measuring chromosome, gene or specific nucleotide sequence copy numbers using snp array
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
DE102008025656B4 (de) 2008-05-28 2016-07-28 Genxpro Gmbh Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung
GB0810051D0 (en) 2008-06-02 2008-07-09 Oxford Biodynamics Ltd Method of diagnosis
KR20110042050A (ko) 2008-06-05 2011-04-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 폴리머좀, 콜로이드좀, 리포좀 및 유체 액적과 관련된 다른 종
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US8388945B2 (en) 2008-07-11 2013-03-05 Eth Zurich Degradable microcapsules
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US10722562B2 (en) 2008-07-23 2020-07-28 Immudex Aps Combinatorial analysis and repair
US20100062494A1 (en) 2008-08-08 2010-03-11 President And Fellows Of Harvard College Enzymatic oligonucleotide pre-adenylation
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
WO2013016459A1 (en) * 2011-07-25 2013-01-31 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
MX2011002936A (es) 2008-09-25 2011-04-11 Cephalon Inc Formulaciones liquidas de bendamustina.
US8361299B2 (en) 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
JP5794572B2 (ja) 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 核酸を修飾するためのトランスポゾン末端組成物及び方法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US20100113296A1 (en) 2008-11-05 2010-05-06 Joel Myerson Methods And Kits For Nucleic Acid Analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US20100203647A1 (en) 2008-11-21 2010-08-12 The Rockefeller University Chemical Reporters of Protein Acylation
EP2352852A4 (en) 2008-12-02 2012-10-24 Bio Rad Laboratories DETECTION OF THE CHROMATIN STRUCTURE
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
US10839940B2 (en) 2008-12-24 2020-11-17 New York University Method, computer-accessible medium and systems for score-driven whole-genome shotgun sequence assemble
KR101065807B1 (ko) 2009-01-23 2011-09-19 충남대학교산학협력단 액적 기반의 미세유체 칩을 이용한 마이크로 캡슐 제조방법
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
KR101793744B1 (ko) 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 유동 포커싱 미세유동 장치의 규모 확장
EP2230312A1 (en) 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
DK3002337T3 (en) 2009-03-30 2019-02-18 Illumina Inc ANALYSIS OF EXPRESSION OF GENES IN SINGLE CELLS
CA3018687C (en) 2009-04-02 2021-07-13 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
EP2427572B1 (en) 2009-05-01 2013-08-28 Illumina, Inc. Sequencing methods
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
FR2945545B1 (fr) 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
FR2945819B1 (fr) 2009-05-19 2011-06-17 Commissariat Energie Atomique Dispositif et procede d'isolement de cibles biologiques ou chimiques
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
SG177369A1 (en) 2009-06-26 2012-02-28 Harvard College Fluid injection
US20110028412A1 (en) 2009-08-03 2011-02-03 Cappellos, Inc. Herbal enhanced analgesic formulations
US20110033548A1 (en) 2009-08-05 2011-02-10 E.I. Du Pont De Nemours And Company Degradable crosslinked aminated dextran microspheres and methods of use
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
RU2552215C2 (ru) 2009-09-01 2015-06-10 Конинклейке Филипс Электроникс Н.В. Устройство и способ отбора нуклеиновых кислот с помощью микроматриц
KR20120089661A (ko) 2009-09-02 2012-08-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 분출 및 다른 기술을 사용하여 생성되는 다중 에멀션
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
GB0918564D0 (en) * 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
EP3842150A1 (en) 2009-10-27 2021-06-30 President and Fellows of Harvard College Droplet creation techniques
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
CN107028886A (zh) 2009-11-04 2017-08-11 不列颠哥伦比亚大学 含有核酸的脂质粒子及相关的方法
CA2767028A1 (en) * 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
WO2011066476A1 (en) 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US8932812B2 (en) 2009-12-17 2015-01-13 Keygene N.V. Restriction enzyme based whole genome sequencing
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
JP5901046B2 (ja) 2010-02-19 2016-04-06 国立大学法人 千葉大学 ＯＡＴＰ１Ｂ３ｍＲＮＡの新規な選択的スプライシングバリアント
US20110257889A1 (en) 2010-02-24 2011-10-20 Pacific Biosciences Of California, Inc. Sequence assembly and consensus sequence determination
EP2539450B1 (en) 2010-02-25 2016-02-17 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
JP2013523651A (ja) 2010-03-24 2013-06-17 ノースイースタン ユニヴァーシティ 多重コンパートメントのマクロファージ送達
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
FR2958186A1 (fr) 2010-03-30 2011-10-07 Ecole Polytech Dispositif de formation de gouttes dans un circuit microfluide.
WO2011140510A2 (en) 2010-05-06 2011-11-10 Bioo Scientific Corporation Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing
US20110287947A1 (en) 2010-05-18 2011-11-24 University Of Southern California Tethered Conformation Capture
NO2575876T3 (pt) 2010-05-26 2018-05-05
US20120000777A1 (en) 2010-06-04 2012-01-05 The Regents Of The University Of California Devices and methods for forming double emulsion droplet compositions and polymer particles
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
US8703493B2 (en) 2010-06-15 2014-04-22 Src, Inc. Location analysis using fire retardant-protected nucleic acid-labeled tags
US20120003657A1 (en) 2010-07-02 2012-01-05 Samuel Myllykangas Targeted sequencing library preparation by genomic dna circularization
US20120238738A1 (en) * 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
CN103202812B (zh) 2010-08-09 2015-10-28 南京大学 一种制备用于体内递送药理活性物质的蛋白纳米粒的方法
WO2012019765A1 (en) 2010-08-10 2012-02-16 European Molecular Biology Laboratory (Embl) Methods and systems for tracking samples and sample combinations
US20130203675A1 (en) 2010-09-16 2013-08-08 Joseph M. DeSimone Asymmetric biofunctional silyl monomers and particles thereof as prodrugs and delivery vehicles for pharmaceutical, chemical and biological agents
DK2623613T3 (en) 2010-09-21 2016-10-03 Population Genetics Tech Ltd Increasing the reliability of the allele-indications by molecular counting
CN102409048B (zh) * 2010-09-21 2013-10-23 深圳华大基因科技服务有限公司 一种基于高通量测序的dna标签文库构建方法
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9999886B2 (en) 2010-10-07 2018-06-19 The Regents Of The University Of California Methods and systems for on demand droplet generation and impedance based detection
US10392726B2 (en) 2010-10-08 2019-08-27 President And Fellows Of Harvard College High-throughput immune sequencing
WO2012048341A1 (en) * 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
CN102050953B (zh) 2010-10-18 2012-11-07 武汉理工大学 一种可还原降解超分子水凝胶的制备方法
EP2633069B1 (en) 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
US20130225623A1 (en) 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
CA3140602C (en) 2010-11-01 2023-10-03 Bio-Rad Laboratories, Inc. System for forming emulsions
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CN102115789B (zh) * 2010-12-15 2013-03-13 厦门大学 用于第二代高通量测序的核酸标签及其设计方法
EP2652155B1 (en) 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
AU2011348267A1 (en) 2010-12-23 2013-08-01 Sequenom, Inc. Fetal genetic variation detection
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US20120191366A1 (en) 2011-01-20 2012-07-26 Nathaniel Pearson Methods and Apparatus for Assigning a Meaningful Numeric Value to Genomic Variants, and Searching and Assessing Same
US8765455B2 (en) * 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
GB201101429D0 (en) 2011-01-27 2011-03-16 Biocompatibles Uk Ltd Drug delivery system
US9238671B2 (en) 2011-01-28 2016-01-19 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
JP6069224B2 (ja) 2011-01-31 2017-02-01 アプライズ バイオ， インコーポレイテッド 細胞において複数のエピトープを同定する方法
WO2016100976A2 (en) 2014-12-19 2016-06-23 Apprise Bio, Inc. Methods for identifying multiple epitopes in selected sub-populations of cells
JP6017458B2 (ja) * 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
AU2012214312A1 (en) * 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012116250A1 (en) 2011-02-25 2012-08-30 University Of Massachusetts Medical School Monomers and polymers for functional polycarbonates and poly (ester-carbonates) and peg-co-polycarbonate hydrogels
EP2678449A4 (en) 2011-02-25 2015-06-24 Illumina Inc METHODS AND SYSTEMS FOR DETERMINING HAPLOTYPE
EP2681196B1 (en) 2011-03-04 2015-09-09 Life Technologies Corporation Compounds and methods for conjugation of biomolecules
WO2012122546A2 (en) 2011-03-09 2012-09-13 Lawrence Ganeshalingam Biological data networks and methods therefor
AU2012231098B2 (en) * 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
GB2489714B (en) 2011-04-05 2013-11-06 Tracesa Ltd Fluid Identification Method
WO2012150317A1 (en) 2011-05-05 2012-11-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
JP2014516514A (ja) 2011-04-14 2014-07-17 コンプリート・ジェノミックス・インコーポレイテッド 複合核酸配列データの処理および解析
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2012149438A1 (en) 2011-04-28 2012-11-01 Life Technologies Corporation Methods and compositions for multiplex pcr
BR112013027540A2 (pt) 2011-04-28 2016-09-20 Us Dept Veterans Affairs banco de composição de polinucleotídeo, método para produzir um ou mais polinucletídeos de interesse e método para analisar e utilizar dados de sequenciamento
WO2012156918A1 (en) 2011-05-16 2012-11-22 Koninklijke Philips Electronics N.V. Bio-orthogonal drug activation
WO2012157684A1 (ja) 2011-05-16 2012-11-22 地方独立行政法人 大阪府立病院機構 血中ｄｎａの定量的検出による悪性新生物の病勢の進行を評価する方法
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
KR20140034242A (ko) 2011-05-23 2014-03-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 다중 에멀젼을 포함하는 에멀젼의 제어
EP2714938B1 (en) * 2011-05-27 2017-11-15 President and Fellows of Harvard College Methods of amplifying whole genome of a single cell
US8841071B2 (en) * 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US9150916B2 (en) 2011-06-24 2015-10-06 Beat Christen Compositions and methods for identifying the essential genome of an organism
US8927218B2 (en) 2011-06-27 2015-01-06 Flir Systems, Inc. Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
US8975302B2 (en) 2011-07-07 2015-03-10 Life Technologies Corporation Polymer particles, nucleic acid polymer particles and methods of making and using the same
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9605304B2 (en) 2011-07-20 2017-03-28 The Hong Kong Polytechnic University Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
US9012373B2 (en) 2011-08-04 2015-04-21 Sage Science, Inc. Systems and methods for processing fluids
WO2013022961A1 (en) 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
US8846883B2 (en) 2011-08-16 2014-09-30 University Of Southhampton Oligonucleotide ligation
WO2013035114A1 (en) 2011-09-08 2013-03-14 Decode Genetics Ehf Tp53 genetic variants predictive of cancer
AU2012304328B2 (en) 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
EP3964285A1 (en) 2011-09-26 2022-03-09 Thermo Fisher Scientific Geneart GmbH High efficiency, small volume nucleic acid synthesis
CN106423314B (zh) 2011-09-28 2021-03-02 哈佛学院院长等 用于液滴产生和/或流体操纵的系统和方法
US9514272B2 (en) 2011-10-12 2016-12-06 Complete Genomics, Inc. Identification of DNA fragments and structural variations
US9469874B2 (en) 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US20130109576A1 (en) 2011-10-28 2013-05-02 Anthony P. Shuber Methods for detecting mutations
EP2774277A4 (en) 2011-11-04 2015-07-22 Intel Corp SIGNALING FOR THE CONFIGURATION OF COORDINATED DOWNLINK MULTIPORT COMMUNICATIONS
WO2013072790A1 (en) 2011-11-16 2013-05-23 International Business Machines Corporation Microfluidic device with deformable valve
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
ES2953308T3 (es) 2011-12-22 2023-11-10 Harvard College Composiciones y métodos para la detección de analitos
US20150125865A1 (en) 2011-12-23 2015-05-07 Gigagen, Inc. Methods And Apparatuses For Droplet Mixing
JP6170074B2 (ja) 2012-02-09 2017-07-26 ライフ テクノロジーズ コーポレーション 親水性ポリマー粒子およびその製造方法
US9637781B2 (en) 2012-02-14 2017-05-02 The Johns Hopkins University MiRNA analysis methods
WO2013123382A1 (en) 2012-02-15 2013-08-22 Wisconsin Alumni Research Foundation Dithioamine reducing agents
US10011871B2 (en) 2012-02-17 2018-07-03 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
EP2817418B1 (en) * 2012-02-24 2017-10-11 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
NO2694769T3 (pt) 2012-03-06 2018-03-03
US9552458B2 (en) 2012-03-16 2017-01-24 The Research Institute At Nationwide Children's Hospital Comprehensive analysis pipeline for discovery of human genetic variation
US20150125904A1 (en) 2012-03-30 2015-05-07 Massachusetts Institute Of Technology Probe incorporation mediated by enzymes
EP2647426A1 (en) 2012-04-03 2013-10-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
BR112014025695B1 (pt) 2012-04-16 2021-08-03 Biological Dynamics, Inc Preparação de amostra de ácido nucleico
US20130296173A1 (en) 2012-04-23 2013-11-07 Complete Genomics, Inc. Pre-anchor wash
CN109082462B (zh) 2012-05-21 2022-10-28 斯克利普斯研究所 样品制备方法
JP6558830B2 (ja) 2012-06-15 2019-08-14 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム 複数転写産物のハイスループットシークエンシング
JP2015523087A (ja) 2012-07-24 2015-08-13 シーケンタ インコーポレイテッド 配列タグを用いる単一細胞分析
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150005200A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005199A1 (en) 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US20140378349A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) * 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
WO2014047561A1 (en) * 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
FR2996544B1 (fr) 2012-10-08 2015-03-13 Ecole Polytech Circuit microfluidique permettant la mise en contact de gouttes de plusieurs fluides, et procede microfluidique correspondant.
FR2996545B1 (fr) 2012-10-08 2016-03-25 Ecole Polytech Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant.
WO2014062717A1 (en) 2012-10-15 2014-04-24 Life Technologies Corporation Compositions, methods, systems and kits for target nucleic acid enrichment
US10155942B2 (en) 2012-11-05 2018-12-18 Takara Bio Usa, Inc. Barcoding nucleic acids
EP2917366B1 (en) 2012-11-06 2017-08-02 Oxford Nanopore Technologies Limited Quadruplex method
CA2890441A1 (en) 2012-11-07 2014-05-15 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
US20150299772A1 (en) 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
RU2701850C2 (ru) 2012-12-12 2019-10-01 Те Брод Инститьют, Инк. Конструирование систем, способы и оптимизированные направляющие композиции для манипуляции с последовательностями
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2749653A1 (en) 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Molecular coding for analysis of composition of macromolecules and molecular complexes
EP2752664A1 (en) 2013-01-07 2014-07-09 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Label-free method for the detection of analytes
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
GB202020510D0 (en) 2013-01-17 2021-02-03 Edico Genome Corp Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
KR101984699B1 (ko) 2013-01-24 2019-05-31 삼성전자주식회사 핵산 분석용 미세 유체 시스템
EP3473905B1 (en) 2013-01-25 2020-07-29 Bio-rad Laboratories, Inc. System and method for performing droplet inflation
US10381106B2 (en) 2013-01-28 2019-08-13 Hasso-Plattner-Institut Fuer Softwaresystemtechnik Gmbh Efficient genomic read alignment in an in-memory database
US11110458B2 (en) 2013-02-01 2021-09-07 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
WO2014138154A1 (en) 2013-03-06 2014-09-12 President And Fellows Of Harvard College Devices and methods for forming relatively monodisperse droplets
SG11201507087WA (en) 2013-03-08 2015-10-29 Bio Rad Laboratories Compositions, methods and systems for polymerase chain reaction assays
EP3919617A1 (en) 2013-03-13 2021-12-08 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US10612088B2 (en) 2013-03-14 2020-04-07 The Broad Institute, Inc. Massively multiplexed RNA sequencing
US20140272996A1 (en) 2013-03-15 2014-09-18 Bio-Rad Laboratories, Inc. Droplet generator with collection tube
US11299718B2 (en) 2013-03-15 2022-04-12 4basebio Sl Methods for amplification and sequencing using thermostable TthPrimPol
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
AU2014233373B2 (en) 2013-03-15 2019-10-24 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
CA2907050C (en) 2013-03-15 2023-09-26 Prognosys Biosciences, Inc. Methods for detecting peptide/mhc/tcr binding
EP2981349A4 (en) 2013-04-02 2016-11-16 Raindance Technologies Inc SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS
EP2994559B1 (en) 2013-05-09 2020-07-08 Bio-rad Laboratories, Inc. Magnetic immuno digital pcr assay
EP4321628A3 (en) 2013-05-23 2024-04-24 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
WO2014200767A1 (en) 2013-06-12 2014-12-18 The General Hospital Corporation Methods, kits, and systems for multiplexed detection of target molecules and uses thereof
WO2014201273A1 (en) 2013-06-12 2014-12-18 The Broad Institute, Inc. High-throughput rna-seq
RU2716420C2 (ru) 2013-06-17 2020-03-11 Те Брод Инститьют Инк. Доставка и применение систем crispr-cas, векторов и композиций для целенаправленного воздействия и терапии в печени
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
MX361481B (es) 2013-06-27 2018-12-06 10X Genomics Inc Composiciones y metodos para procesamiento de muestras.
EP3019618B1 (en) 2013-07-12 2018-10-31 University of South Alabama Minimal piggybac vectors for genome integration
CN103394410B (zh) 2013-07-25 2016-04-20 博奥生物集团有限公司 一种位置可调节的智能磁力架
GB2516684A (en) 2013-07-30 2015-02-04 Sphere Fluidics Ltd Microfluidic devices and systems
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
JP6700173B2 (ja) 2013-09-24 2020-05-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ターゲット検出方法及びシステム
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
WO2015053943A1 (en) 2013-10-09 2015-04-16 Stc.Unm Synthetic long read dna sequencing
US9937495B2 (en) 2013-10-28 2018-04-10 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US20140315755A1 (en) 2013-12-26 2014-10-23 Tao Chen Genome-wide Antisense Oligonucleotide and RNAi
CA2935122C (en) 2013-12-30 2023-09-19 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
KR101464100B1 (ko) 2014-01-29 2014-11-21 성균관대학교산학협력단 생체 적용 가능한 리포솜-핵산형광 나노 융합체, 이의 응용, 및 이의 제조방법
CN106103713B (zh) 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
CN105531408B (zh) 2014-02-13 2019-09-10 生物辐射实验室股份有限公司 染色体构象划分产物捕获
US20160376663A1 (en) 2014-02-27 2016-12-29 Igenomx International Genomics Corporation Methods for analysis of somatic mobile elements, and uses thereof
DE202015009494U1 (de) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidische Vorrichtungen und Systeme zur Einkapselung und Partitionierung von Reagenzien, und deren Anwendungen
AU2015247779B2 (en) 2014-04-15 2021-06-24 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased DNA input tolerance
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
DK3299469T3 (da) 2014-04-21 2020-03-30 Harvard College Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US10526601B2 (en) 2014-05-23 2020-01-07 Digenomix Corporation Haploidome determination by digitized transposons
EP3152232B1 (en) 2014-06-06 2019-11-13 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
AU2015273480A1 (en) 2014-06-11 2016-12-08 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
US9534215B2 (en) 2014-06-11 2017-01-03 Life Technologies Corporation Systems and methods for substrate enrichment
EP3155426B1 (en) 2014-06-13 2023-07-19 Immudex ApS General detection and isolation of specific cells by binding of labeled molecules
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
EP3161162A4 (en) 2014-06-26 2018-01-10 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN106574298A (zh) 2014-06-26 2017-04-19 10X基因组学有限公司 用于样品分析的方法和组合物
AU2015289414B2 (en) 2014-07-18 2021-07-08 Illumina, Inc. Non-invasive prenatal diagnosis of fetal genetic condition using cellular DNA and cell free DNA
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
CN105335116B (zh) 2014-07-30 2018-11-09 联想(北京)有限公司 一种显示控制方法及电子设备
WO2016033251A2 (en) 2014-08-26 2016-03-03 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP4105337A1 (en) 2014-09-09 2022-12-21 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
ES2727656T3 (es) 2014-09-15 2019-10-17 Abvitro Llc Secuenciación de alto rendimiento de banco de nucleótidos
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
SG11201705425SA (en) 2015-01-13 2017-08-30 10X Genomics Inc Systems and methods for visualizing structural variation and phasing information
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2974306A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
MX2017010142A (es) 2015-02-09 2017-12-11 10X Genomics Inc Sistemas y metodos para determinar variacion estructural y ajuste de fases con datos de recuperacion de variantes.
PT3256604T (pt) 2015-02-10 2020-05-18 Illumina Inc Métodos e composições para analisar componentes celulares
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
US10981167B2 (en) 2015-03-18 2021-04-20 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
US20160289769A1 (en) 2015-03-30 2016-10-06 Verily Life Sciences Llc Methods for Combining Single Cell Profiling with Combinatorial Nanoparticle Conjugate Library Screening and In Vivo Diagnostic System
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US20180195060A1 (en) 2015-04-20 2018-07-12 Bgi Shenzhen Method for constructing long fragment dna library
EP3708256B1 (en) 2015-04-22 2022-11-30 Stilla Technologies Contact-less priming method for loading a solution in a microfluidic device and associated system
US20160314242A1 (en) 2015-04-23 2016-10-27 10X Genomics, Inc. Sample indexing methods and compositions for sequencing applications
JP2018518950A (ja) 2015-05-18 2018-07-19 １０エックス ジェノミクス， インコーポレイテッド 生物医学的な反応及び分析で使用するための移動固相組成物
WO2016187179A1 (en) 2015-05-18 2016-11-24 10X Genomics, Inc. Stabilized reducing agents and methods using same
CN107615283B (zh) 2015-05-26 2022-07-05 加利福尼亚太平洋生物科学股份有限公司 用于二倍体基因组组装和单倍型序列重建的方法、软件和系统
WO2016187717A1 (en) 2015-05-26 2016-12-01 Exerkine Corporation Exosomes useful for genome editing
US20180087050A1 (en) 2015-05-27 2018-03-29 Jianbiao Zheng Methods of inserting molecular barcodes
EP3314015A1 (en) 2015-06-24 2018-05-02 Oxford Biodynamics Limited Detection of chromosome interactions
RU2018105835A (ru) 2015-07-17 2019-08-19 Президент Энд Фэллоуз Оф Харвард Коллидж Способы амплификации последовательностей нуклеиновых кислот
US10934636B2 (en) 2015-08-12 2021-03-02 CeMM—FORSCHUNGSZENTRUM FÜR MOLEKULARE MEDIZIN GmbH Methods for studying nucleic acids
WO2017034970A1 (en) 2015-08-21 2017-03-02 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
KR20180085717A (ko) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
WO2017066908A1 (zh) 2015-10-19 2017-04-27 安诺优达基因科技(北京)有限公司 构建高分辨率、大信息量单细胞Hi-C文库的方法
WO2017070056A1 (en) 2015-10-20 2017-04-27 10X Genomics, Inc. Methods and systems for high throughput single cell genetic manipulation
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
JP7064439B2 (ja) 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
US20170145476A1 (en) 2015-11-19 2017-05-25 10X Genomics, Inc. Transformable tagging compositions, methods, and processes incorporating same
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
US11965891B2 (en) 2015-12-30 2024-04-23 Bio-Rad Laboratories, Inc. Digital protein quantification
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017139690A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
US20190078150A1 (en) 2016-03-01 2019-03-14 Universal Sequencing Technology Corporation Methods and Kits for Tracking Nucleic Acid Target Origin for Nucleic Acid Sequencing
SG11201807444PA (en) 2016-03-10 2018-09-27 Univ Leland Stanford Junior Transposase-mediated imaging of the accessible genome
WO2017180420A1 (en) 2016-04-11 2017-10-19 Board Of Regents, The University Of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
AU2017311306A1 (en) 2016-08-10 2019-03-14 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic DNA fragments
US10688494B2 (en) 2016-08-23 2020-06-23 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
US10858699B2 (en) 2016-08-30 2020-12-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
US20210285038A1 (en) 2016-08-31 2021-09-16 President And Fellows Of Harvard College Methods of Whole Genome Digital Amplification
CN109923216A (zh) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
US20180080021A1 (en) 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CN114875125A (zh) 2016-10-19 2022-08-09 10X基因组学有限公司 用于条形码化单个细胞或细胞群的核酸分子的方法和系统
US11434483B2 (en) 2016-12-07 2022-09-06 Mgi Tech Co., Ltd. Method for constructing single cell sequencing library and use thereof
WO2018118971A1 (en) 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
SG10201911905QA (en) 2016-12-29 2020-01-30 Illumina Inc Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
US20200232022A1 (en) 2017-01-06 2020-07-23 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US20210087610A9 (en) 2017-01-12 2021-03-25 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10347365B2 (en) 2017-02-08 2019-07-09 10X Genomics, Inc. Systems and methods for visualizing a pattern in a dataset
GB201704402D0 (en) 2017-03-20 2017-05-03 Blacktrace Holdings Ltd Single cell DNA sequencing
SG11201907762RA (en) 2017-03-24 2019-10-30 Nat Univ Singapore Methods for multiplex detection of molecules
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
WO2018191701A1 (en) 2017-04-14 2018-10-18 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
CN110799679A (zh) 2017-06-20 2020-02-14 10X基因组学有限公司 用于改善液滴稳定的方法和系统
US20200131506A1 (en) 2017-06-21 2020-04-30 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
CN108336542B (zh) 2017-06-23 2020-02-21 番禺得意精密电子工业有限公司 电连接器
CA3067048C (en) 2017-08-01 2024-03-05 Illumina, Inc. Hydrogel beads for nucleotide sequencing
US9946577B1 (en) 2017-08-14 2018-04-17 10X Genomics, Inc. Systems and methods for distributed resource management
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
EP3691703A1 (en) 2017-10-04 2020-08-12 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US20190127731A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods for preparing nucleic acid molecules
WO2019084328A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS FOR PREPARING NUCLEIC ACID MOLECULES
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
WO2019118355A1 (en) 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
WO2019148042A1 (en) 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
US20210213413A1 (en) 2018-02-05 2021-07-15 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
SG11202008080RA (en) 2018-02-22 2020-09-29 10X Genomics Inc Ligation mediated analysis of nucleic acids
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019169347A1 (en) 2018-03-02 2019-09-06 10X Genomics, Inc. Systems and apparatus for holding plates
WO2019191321A1 (en) 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US20190345636A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20190352717A1 (en) 2018-05-18 2019-11-21 10X Genomics, Inc. Targeted non-invasive prenatal testing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020006183A1 (en) 2018-06-28 2020-01-02 10X Genomics, Inc. Systems and methods for visualization of single-cell resolution characteristics
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US20200033366A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US20200263232A1 (en) 2018-08-03 2020-08-20 10X Genomics, Inc. Methods and systems to minimize barcode exchange
US20200056223A1 (en) 2018-08-20 2020-02-20 10X Genomics, Inc. Compositions and methods for cellular processing
EP3906318A1 (en) 2019-01-06 2021-11-10 10X Genomics, Inc. Methods and systems for enrichment of barcodes

Also Published As

Publication number Publication date
US10150963B2 (en) 2018-12-11
CA2900543A1 (en) 2014-08-14
US20170342404A1 (en) 2017-11-30
CA2900481A1 (en) 2014-08-14
US20170362587A1 (en) 2017-12-21
EP2954065B1 (en) 2021-07-28
WO2014124336A2 (en) 2014-08-14
JP2023063359A (ja) 2023-05-09
US10150964B2 (en) 2018-12-11
AU2014214682B2 (en) 2018-07-26
EP2954104A4 (en) 2016-08-24
DK2954065T3 (da) 2021-09-06
EP3998342A1 (en) 2022-05-18
US20140235506A1 (en) 2014-08-21
US20140227684A1 (en) 2014-08-14
EP3862435A1 (en) 2021-08-11
KR20150119047A (ko) 2015-10-23
US11193121B2 (en) 2021-12-07
KR20230003659A (ko) 2023-01-06
WO2014124338A1 (en) 2014-08-14
CN108753766A (zh) 2018-11-06
JP2021038272A (ja) 2021-03-11
KR102190198B1 (ko) 2020-12-14
CA2900543C (en) 2023-01-31
EP2954104A1 (en) 2015-12-16
CN105102697A (zh) 2015-11-25
US9644204B2 (en) 2017-05-09
US20190276817A1 (en) 2019-09-12
EP2954104B1 (en) 2020-09-16
JP2016511243A (ja) 2016-04-14
AU2014214682A1 (en) 2015-08-13
US9388465B2 (en) 2016-07-12
US20140228255A1 (en) 2014-08-14
KR20200140929A (ko) 2020-12-16
EP2954065A4 (en) 2016-08-24
US20160304860A1 (en) 2016-10-20
JP2019107035A (ja) 2019-07-04
WO2014124336A3 (en) 2014-10-23
US20220154175A1 (en) 2022-05-19
EP2954065A2 (en) 2015-12-16

Similar Documents

Publication Publication Date Title
BR112015019159A2 (pt) geração de código de barras de polinucleotídeos
BR112017017872A2 (pt) ?sistema de geração e distribuição de energia para unidades marítimas de perfuração?
BR112018013065A2 (pt) composições e métodos para o tratamento de hemoglobinopatias
BR112018076090A2 (pt) vírus adeno-associados variantes e métodos de utilização
CL2016000839A1 (es) Composiciones para modular la expresión de c9orf72.
BR112017010331A2 (pt) cobertura com articulação com pivô múltiplo
UY35821A (es) Compuesto heterocíclico
CR20150462A (es) Inhibidores de erk y sus usos
GB2547308B (en) Acid resistant urease inhibitor containing fertilizer compositions
EA201591137A1 (ru) Ингибиторы mk2 и их применения
BR112018003784A2 (pt) nanopartículas de polinucleotídeo para a modulação da expressão gênica e sua utilização
UY34484A (es) Benzotienilo-pirrolotriazinas disustituidas y sus usos
BR112018006034A2 (pt) compostos bicíclicos como inibidores de atx
UY33504A (es) N-etil-n-fenil-1,2-dihidro-4-hidroxi-5-cloro-1-metil-2-oxoquinolina-3-carboxamida deuterada, sales y usos de la misma
ITUB20152606A1 (it) Custodia di connettore protetta contro la corrosione e l’erosione
BR112015023473A2 (pt) mutações acetil-coa carboxilase
DK3452690T3 (da) Kemiske varmekilder til anvendelse i borehulsanvendelser
DK3577406T3 (da) Væskekølesystemer til varmegenererende indretninger
UY35804A (es) Recubrimiento de alginato para tratamiento de semillas
BR112014022409A2 (pt) vetor de expressão que compreende um polinucleotídeo codificante de uma glutamina-sintetase modificada e um método para a preparação de uma proteína alvo oue emprega o mesmo
EP3652663A4 (en) IMMUNO-ONCOLOGICAL APPLICATIONS IMPLEMENTING NEXT GENERATION SEQUENCING
BR112017022391A2 (pt) composições e métodos para a fiscalização de câncer de próstata
AR104591A1 (es) Piroglutamato de vortioxetina
DK3435912T3 (da) Systemer og anordninger som omfatter ptfe tandskruer
BR112015003341A2 (pt) fonte de alimentação e multiplicação de voltagem para sistemas submarinos submersos com base em sistema de proteção catódica.

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B25G Requested change of headquarter approved

Owner name: 10X GENOMICS, INC. (US)

B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements