MX361481B - Composiciones y metodos para procesamiento de muestras. - Google Patents

Composiciones y metodos para procesamiento de muestras.

Info

Publication number
MX361481B
MX361481B MX2015016968A MX2015016968A MX361481B MX 361481 B MX361481 B MX 361481B MX 2015016968 A MX2015016968 A MX 2015016968A MX 2015016968 A MX2015016968 A MX 2015016968A MX 361481 B MX361481 B MX 361481B
Authority
MX
Mexico
Prior art keywords
partition
methods
barcode molecules
nucleic acid
compositions
Prior art date
Application number
MX2015016968A
Other languages
English (en)
Other versions
MX2015016968A (es
Inventor
Benjamin Hindson
Serge Saxonov
HINDSON Christopher
Schnall-Levin Michael
NESS Kevin
Jarosz Mirna
Merrill Landon
Price Andrew
Hardenbol Paul
MASQUELIER Donald
Li Yuan
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52142836&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX361481(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from PCT/US2013/054797 external-priority patent/WO2014028537A1/en
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of MX2015016968A publication Critical patent/MX2015016968A/es
Publication of MX361481B publication Critical patent/MX361481B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Abstract

La presente invención se refiere a métodos y composiciones para el procesamiento de muestras, particularmente para aplicaciones de secuenciación. En la presente descripción se incluyen composiciones de cuentas, tal como diversas bibliotecas de cuentas unidas a grandes números de oligonucleótidos que contienen códigos de barras. Frecuentemente, las cuentas proporcionadas en la presente son degradables. Por ejemplo, pueden contener enlaces de disulfuro que son susceptibles a agentes reductores. Los métodos proporcionados en la presente incluyen métodos para producir bibliotecas de cuentas marcadas con código de barras así como métodos para combinar las cuentas con una muestra, tal como al usar un dispositivo microfluídico.
MX2015016968A 2013-06-27 2014-06-26 Composiciones y metodos para procesamiento de muestras. MX361481B (es)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201361840403P 2013-06-27 2013-06-27
US201361844804P 2013-07-10 2013-07-10
PCT/US2013/054797 WO2014028537A1 (en) 2012-08-14 2013-08-13 Microcapsule compositions and methods
US13/966,150 US20140155295A1 (en) 2012-08-14 2013-08-13 Capsule array devices and methods of use
US201361896060P 2013-10-26 2013-10-26
US201361909974P 2013-11-27 2013-11-27
US201461937344P 2014-02-07 2014-02-07
US201461940318P 2014-02-14 2014-02-14
US201461991018P 2014-05-09 2014-05-09
PCT/US2014/044398 WO2014210353A2 (en) 2013-06-27 2014-06-26 Compositions and methods for sample processing

Publications (2)

Publication Number Publication Date
MX2015016968A MX2015016968A (es) 2016-08-08
MX361481B true MX361481B (es) 2018-12-06

Family

ID=52142836

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015016968A MX361481B (es) 2013-06-27 2014-06-26 Composiciones y metodos para procesamiento de muestras.

Country Status (9)

Country Link
EP (3) EP3013957B2 (es)
JP (1) JP6563912B2 (es)
KR (4) KR102436171B1 (es)
CN (3) CN110592182B (es)
AU (3) AU2014302277A1 (es)
BR (1) BR112015032512A8 (es)
CA (1) CA2915499A1 (es)
MX (1) MX361481B (es)
WO (1) WO2014210353A2 (es)

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
JP6375230B2 (ja) 2012-02-27 2018-08-15 セルラー リサーチ， インコーポレイテッド 分子計数のための組成物およびキット
ES2776673T3 (es) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Métodos y usos para etiquetas moleculares
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3862435A1 (en) 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
CN111662960B (zh) 2013-06-25 2024-04-12 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
EP3013957B2 (en) 2013-06-27 2022-05-11 10X Genomics, Inc. Compositions and methods for sample processing
KR20230074639A (ko) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015200541A1 (en) 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
CN106795553B (zh) * 2014-06-26 2021-06-04 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US20150376605A1 (en) * 2014-06-26 2015-12-31 10X Genomics, Inc. Methods and Compositions for Sample Analysis
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP3244992B1 (en) * 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016149418A1 (en) * 2015-03-18 2016-09-22 Cellular Research, Inc. Methods and compositions for labeling targets and haplotype phasing
US11535882B2 (en) 2015-03-30 2022-12-27 Becton, Dickinson And Company Methods and compositions for combinatorial barcoding
EP3283629A4 (en) * 2015-04-17 2018-08-29 President and Fellows of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10788452B2 (en) 2015-04-21 2020-09-29 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
JP2018518950A (ja) * 2015-05-18 2018-07-19 １０エックス ジェノミクス， インコーポレイテッド 生物医学的な反応及び分析で使用するための移動固相組成物
CN107532218A (zh) * 2015-05-18 2018-01-02 10X基因组学有限公司 稳定化还原剂及其使用方法
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
WO2016205728A1 (en) 2015-06-17 2016-12-22 Massachusetts Institute Of Technology Crispr mediated recording of cellular events
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017075451A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting pou2af1
KR101651817B1 (ko) 2015-10-28 2016-08-29 대한민국 Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트
EP3368689B1 (en) 2015-10-28 2020-06-17 The Broad Institute, Inc. Composition for modulating immune responses by use of immune cell gene signature
WO2017075465A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses by detecting and targeting gata3
KR20180097536A (ko) 2015-11-04 2018-08-31 아트레카, 인크. 단일 세포와 연관된 핵산의 분석을 위한 핵산 바코드의 조합 세트
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
CN108779460B (zh) * 2015-12-07 2023-08-01 睿恩达思技术公司 在分配中使用微粒的多路技术
JP7129343B2 (ja) 2016-05-02 2022-09-01 ベクトン・ディキンソン・アンド・カンパニー 正確な分子バーコーディング
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
AU2017295717B2 (en) 2016-07-15 2021-06-24 The Regents Of The University Of California Methods of producing nucleic acid libraries
US11225689B2 (en) 2016-08-17 2022-01-18 The Broad Institute, Inc. Method for determination and identification of cell signatures and cell markers
WO2018049025A2 (en) 2016-09-07 2018-03-15 The Broad Institute Inc. Compositions and methods for evaluating and modulating immune responses
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
JP2019531730A (ja) * 2016-09-28 2019-11-07 ジェネラル オートメーション ラボ テクノロジーズ インコーポレイテッド 細菌群の関係決定及び他のハイスループット微生物学適用高分解能システム、キット、装置、並びに方法
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
CN117056774A (zh) 2016-11-08 2023-11-14 贝克顿迪金森公司 用于细胞标记分类的方法
KR101909460B1 (ko) * 2016-11-21 2018-10-22 한국과학기술연구원 프라이머가 고정된 하이드로겔 미세입자 및 이를 이용한 핵산 증폭 방법
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
ES2961580T3 (es) 2017-01-13 2024-03-12 Cellular Res Inc Revestimiento hidrófilo de canales de fluidos
JP7033602B2 (ja) 2017-01-27 2022-03-10 エフ．ホフマン－ラ ロシュ アーゲー ロングレンジ配列決定のためのバーコードを付けられたｄｎａ
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
US11913075B2 (en) 2017-04-01 2024-02-27 The Broad Institute, Inc. Methods and compositions for detecting and modulating an immunotherapy resistance gene signature in cancer
EP3610266A4 (en) 2017-04-12 2021-04-21 Massachusetts Eye and Ear Infirmary TUMOR SIGNATURE OF METASTASIS, COMPOSITIONS OF SUBSTANCES AND USES THEREOF
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
CN111148849A (zh) * 2017-05-26 2020-05-12 阿布维托有限责任公司 高通量多核苷酸文库测序和转录组分析
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4345172A2 (en) 2017-06-05 2024-04-03 Becton, Dickinson and Company Sample indexing for single cells
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
EP3638218A4 (en) 2017-06-14 2021-06-09 The Broad Institute, Inc. COMPOSITIONS AND METHOD OF TARGETING COMPLEMENTING COMPONENT 3 FOR INHIBITION OF TUMOR GROWTH
SG11201911869XA (en) 2017-08-01 2020-01-30 Illumina Inc Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
WO2019060830A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
US20200255828A1 (en) 2017-10-04 2020-08-13 The Broad Institute, Inc. Methods and compositions for altering function and structure of chromatin loops and/or domains
US11680296B2 (en) 2017-10-16 2023-06-20 Massachusetts Institute Of Technology Mycobacterium tuberculosis host-pathogen interaction
WO2019079653A1 (en) 2017-10-19 2019-04-25 Ultima Genomics, Inc. METHODS OF TREATING APPARATUSED END SEQUENCES
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019113235A1 (en) * 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides
WO2019126789A1 (en) * 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
JP7296969B2 (ja) 2018-01-12 2023-06-23 クラレット バイオサイエンス， エルエルシー 核酸を解析するための方法および組成物
WO2019157529A1 (en) * 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019213660A2 (en) 2018-05-04 2019-11-07 The Broad Institute, Inc. Compositions and methods for modulating cgrp signaling to regulate innate lymphoid cell inflammatory responses
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US20210371932A1 (en) 2018-06-01 2021-12-02 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
AU2019280712A1 (en) 2018-06-06 2021-01-07 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
WO2019241273A1 (en) 2018-06-11 2019-12-19 The Broad Institute, Inc. Lineage tracing using mitochondrial genome mutations and single cell genomics
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
US20220401460A1 (en) 2018-10-10 2022-12-22 Dana-Farber Cancer Institute, Inc. Modulating resistance to bcl-2 inhibitors
WO2020077236A1 (en) 2018-10-12 2020-04-16 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
US20210379057A1 (en) 2018-10-16 2021-12-09 Massachusetts Institute Of Technology Nutlin-3a for use in treating a mycobacterium tuberculosis infection
AU2019364545A1 (en) * 2018-10-26 2021-04-29 Illumina, Inc. Modulating polymer beads for DNA processing
US11512356B2 (en) * 2018-11-08 2022-11-29 Tokitae Llc Systems and methods for particle multiplexing in droplets
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
WO2020123320A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Imaging system hardware
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
WO2020131586A2 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Methods for identifying neoantigens
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US20220119871A1 (en) 2019-01-28 2022-04-21 The Broad Institute, Inc. In-situ spatial transcriptomics
JP2022519324A (ja) * 2019-02-11 2022-03-22 ウルティマ・ゲノミクス・インコーポレーテッド 核酸分析方法
US20230053540A1 (en) 2019-02-19 2023-02-23 Massachusetts Institute Of Technology Treatment of liver injury
WO2020185791A1 (en) * 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US20220154282A1 (en) 2019-03-12 2022-05-19 The Broad Institute, Inc. Detection means, compositions and methods for modulating synovial sarcoma cells
US20220143148A1 (en) 2019-03-14 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating cgrp signaling to regulate intestinal innate lymphoid cells
US20220152148A1 (en) 2019-03-18 2022-05-19 The Broad Institute, Inc. Modulation of type 2 immunity by targeting clec-2 signaling
US20220142948A1 (en) 2019-03-18 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
WO2021030627A1 (en) 2019-08-13 2021-02-18 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
EP4041310A1 (en) 2019-10-10 2022-08-17 1859, Inc. Methods and systems for microfluidic screening
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
WO2021127610A1 (en) 2019-12-20 2021-06-24 EDWARD Via COLLEGE OF OSTEOPATHIC MEDICINE Cancer signatures, methods of generating cancer signatures, and uses thereof
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
IL272390A (en) 2020-01-30 2021-08-31 Yeda Res & Dev Cancer treatment methods
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
IL272586A (en) 2020-02-10 2021-08-31 Yeda Res & Dev A method for cell cluster analysis
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4242325A3 (en) 2020-04-22 2023-10-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4158054A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
IL278473A (en) 2020-11-03 2022-06-01 Yeda Res & Dev Methods for diagnosing and determining treatment in multiple myeloma
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022150662A1 (en) 2021-01-08 2022-07-14 10X Genomics, Inc. Methods for generating antigen-binding molecules from single cells
KR102320966B1 (ko) * 2021-02-15 2021-11-04 대한민국(식품의약품안전처장) 차세대염기서열분석법을 이용한 초위성체 마커 탐색 접근법
WO2022192419A2 (en) 2021-03-09 2022-09-15 Massachusetts Institute Of Technology Methods of treating inflammatory bowel disease (ibd) with anti- tnf-blockade
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2024076728A1 (en) 2022-10-06 2024-04-11 Dana-Farber Cancer Institute, Inc. Cyclic nucleotides and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
WO2006030993A1 (en) * 2004-09-14 2006-03-23 Jin-Ho Choy Information code system using dna sequences
JP2009513948A (ja) * 2005-09-16 2009-04-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 検体検出のための比色バイオバーコード増幅アッセイ
CA2653321A1 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc. Biochemical analysis of partitioned cells
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN101241126A (zh) * 2008-03-14 2008-08-13 东华大学 一种生物条形码探针的制备方法
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
CN103952482A (zh) * 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
WO2011140510A2 (en) * 2010-05-06 2011-11-10 Bioo Scientific Corporation Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing
US20110319290A1 (en) * 2010-06-08 2011-12-29 Nugen Technologies, Inc. Methods and Compositions for Multiplex Sequencing
DK2625320T3 (da) * 2010-10-08 2019-07-01 Harvard College High-throughput enkeltcellestregkodning
US9163281B2 (en) * 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
AU2012212148B8 (en) 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
EP3789498A1 (en) * 2011-04-25 2021-03-10 Bio-rad Laboratories, Inc. Methods for nucleic acid analysis
US8841071B2 (en) * 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2013126741A1 (en) * 2012-02-24 2013-08-29 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
AU2013293240A1 (en) 2012-07-24 2015-03-05 Adaptive Biotechnologies Corp. Single cell analysis using sequence tags
US20140378322A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2749653A1 (en) 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Molecular coding for analysis of composition of macromolecules and molecular complexes
EP3013957B2 (en) 2013-06-27 2022-05-11 10X Genomics, Inc. Compositions and methods for sample processing
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids

Also Published As

Publication number Publication date
CN117568449A (zh) 2024-02-20
KR102366116B1 (ko) 2022-02-23
KR20210013668A (ko) 2021-02-04
CN110592182B (zh) 2023-11-28
CN110592182A (zh) 2019-12-20
KR102212234B1 (ko) 2021-02-05
EP3013957B1 (en) 2018-09-26
KR20160032723A (ko) 2016-03-24
JP6563912B2 (ja) 2019-08-21
KR20220122780A (ko) 2022-09-02
MX2015016968A (es) 2016-08-08
EP3013957A4 (en) 2016-08-24
BR112015032512A8 (pt) 2020-01-07
WO2014210353A3 (en) 2015-07-09
AU2023203879A1 (en) 2023-07-13
CN105492607B (zh) 2019-07-02
EP4357493A2 (en) 2024-04-24
AU2020244615B2 (en) 2023-06-08
CA2915499A1 (en) 2014-12-31
KR102436171B1 (ko) 2022-08-24
KR20220025265A (ko) 2022-03-03
AU2020244615A1 (en) 2020-11-05
CN105492607A (zh) 2016-04-13
WO2014210353A2 (en) 2014-12-31
KR102642680B1 (ko) 2024-03-04
AU2014302277A1 (en) 2015-12-24
EP3013957B2 (en) 2022-05-11
BR112015032512A2 (pt) 2017-07-25
EP3013957A2 (en) 2016-05-04
JP2016526889A (ja) 2016-09-08
EP3467160A1 (en) 2019-04-10

Similar Documents

Publication Publication Date Title
MX361481B (es) Composiciones y metodos para procesamiento de muestras.
MX2016016904A (es) Analisis de secuencias de acidos nucleicos.
WO2018119447A3 (en) Methods and systems for processing polynucleotides
MX364957B (es) Composiciones y metodos para microcapsulas.
WO2016130868A3 (en) Materials and methods to analyze rna isoforms in transcriptomes
WO2016126871A3 (en) Sequencing of nucleic acids via barcoding in discrete entities
WO2016033251A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
MX2018006430A (es) Preparacion de muestras de acido nucleico.
WO2016205745A3 (en) Cell sorting
WO2015119941A3 (en) Genome fractioning
WO2015200893A3 (en) Methods of analyzing nucleic acids from individual cells or cell populations
GB2513793A (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP4235677A3 (en) Processes and systems for nucleic acid sequence assembly
MX2016016898A (es) Metodos y composiciones para analisis de muestras.
WO2012149042A3 (en) Methods and compositions for nucleic acid analysis
WO2015188839A3 (en) Detection and isolation of specific cells by binding of lebeled molecules
EA201692157A1 (ru) Синтез двухцепочечных нуклеиновых кислот
SG194233A1 (en) Identification of polynucleotides associated with a sample
NZ731116A (en) Method for the enrichment of circulating tumor dna
GB2525804A (en) Methods and compositions for classification of samples
WO2014145820A3 (en) Multiple tagging of long dna fragments
PH12017501967A1 (en) Maize plant dbn9936 and method for use in detecting nucleic acid sequence thereof
EP3201350A4 (en) Method for nucleic acid analysis directly from an unpurified biological sample
MX2016013216A (es) Dispositivos mejorados para la separacion de materiales biologicos.
EP3216852A4 (en) Method for introducing nucleic acid, method for detecting nucleic acid, method for analyzing biological component, array device for biological component assay, and kit for analyzing biological component

Legal Events

Date Code Title Description
FG Grant or registration