EA201170933A1 - Анализ нуклеиновых кислот, полученных из единичных клеток - Google Patents

Анализ нуклеиновых кислот, полученных из единичных клеток

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Publication number
EA201170933A1
EA201170933A1 EA201170933A EA201170933A EA201170933A1 EA 201170933 A1 EA201170933 A1 EA 201170933A1 EA 201170933 A EA201170933 A EA 201170933A EA 201170933 A EA201170933 A EA 201170933A EA 201170933 A1 EA201170933 A1 EA 201170933A1
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EA
Eurasian Patent Office
Prior art keywords
nucleic acids
single cells
analysis
amplification
methods
Prior art date
Application number
EA201170933A
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English (en)
Other versions
EA020593B1 (ru
Inventor
Эми Хэмилтон
Минь Линь
Ален Мир
Мартин Пепжик
Original Assignee
Флуидигм Корпорейшн
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Application filed by Флуидигм Корпорейшн filed Critical Флуидигм Корпорейшн
Publication of EA201170933A1 publication Critical patent/EA201170933A1/ru
Publication of EA020593B1 publication Critical patent/EA020593B1/ru

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/137—Ligase Chain Reaction [LCR]
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Abstract

Настоящее изобретение относится к способам анализа геномной ДНК и/или РНК из небольших образцов или даже единичных клеток. Способы анализа геномной ДНК могут включать полногеномную амплификацию (WGA) с последующей преамплификацией и амплификацией выбранных нуклеиновых кислот-мишеней. Способы анализа РНК могут включать обратную транскрипцию желательной РНК с последующей преамплификацией и амплификацией выбранных нуклеиновых кислот-мишеней.
EA201170933A 2009-01-13 2010-01-13 Анализ нуклеиновых кислот, полученных из единичных клеток EA020593B1 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US14441609P 2009-01-13 2009-01-13
US14658309P 2009-01-22 2009-01-22
US28430909P 2009-12-15 2009-12-15
PCT/US2010/020942 WO2010083250A2 (en) 2009-01-13 2010-01-13 Single-cell nucleic acid analysis

Publications (2)

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EA201170933A1 true EA201170933A1 (ru) 2012-02-28
EA020593B1 EA020593B1 (ru) 2014-12-30

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EA201170933A EA020593B1 (ru) 2009-01-13 2010-01-13 Анализ нуклеиновых кислот, полученных из единичных клеток

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US (3) US8628923B2 (ru)
EP (1) EP2379753B1 (ru)
KR (1) KR20110106922A (ru)
CN (1) CN102348811A (ru)
EA (1) EA020593B1 (ru)
IL (1) IL214034A (ru)
SG (2) SG172965A1 (ru)
WO (1) WO2010083250A2 (ru)

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IL214034A0 (en) 2011-08-31
US20160340728A1 (en) 2016-11-24
US9249459B2 (en) 2016-02-02
US20100178655A1 (en) 2010-07-15
EA020593B1 (ru) 2014-12-30
IL214034A (en) 2016-11-30
WO2010083250A3 (en) 2010-11-18
SG183029A1 (en) 2012-08-30
KR20110106922A (ko) 2011-09-29
US9909179B2 (en) 2018-03-06
CN102348811A (zh) 2012-02-08
EP2379753A2 (en) 2011-10-26
US8628923B2 (en) 2014-01-14
WO2010083250A2 (en) 2010-07-22
US20140193812A1 (en) 2014-07-10
SG172965A1 (en) 2011-08-29
EP2379753B1 (en) 2016-12-28
EP2379753A4 (en) 2012-05-30

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