WO2012112925A3 - Quantitative, highly multiplexed detection of nucleic acids - Google Patents

Quantitative, highly multiplexed detection of nucleic acids Download PDF

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Publication number
WO2012112925A3
WO2012112925A3 PCT/US2012/025699 US2012025699W WO2012112925A3 WO 2012112925 A3 WO2012112925 A3 WO 2012112925A3 US 2012025699 W US2012025699 W US 2012025699W WO 2012112925 A3 WO2012112925 A3 WO 2012112925A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
quantitative
highly multiplexed
multiplexed detection
methods
Prior art date
Application number
PCT/US2012/025699
Other languages
French (fr)
Other versions
WO2012112925A9 (en
WO2012112925A2 (en
Inventor
Kris SCABOO
Patrick Martin
Brad TAFT
Original Assignee
NVS Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NVS Technologies, Inc. filed Critical NVS Technologies, Inc.
Priority to JP2013554647A priority Critical patent/JP2014511178A/en
Priority to EP12747077.1A priority patent/EP2675920A4/en
Priority to SG2013060975A priority patent/SG192720A1/en
Priority to CA2827040A priority patent/CA2827040A1/en
Priority to MX2013009429A priority patent/MX2013009429A/en
Priority to CN201280018955.9A priority patent/CN103502471B/en
Priority to BR112013021144A priority patent/BR112013021144A2/en
Priority to AU2012219295A priority patent/AU2012219295A1/en
Priority to KR1020137024679A priority patent/KR20140044309A/en
Priority to EP12849697.3A priority patent/EP2780479A4/en
Priority to MX2014005912A priority patent/MX338076B/en
Priority to PCT/US2012/051236 priority patent/WO2013074163A1/en
Priority to BR112014011937A priority patent/BR112014011937A2/en
Priority to KR1020147016411A priority patent/KR20140094007A/en
Priority to SG11201402341QA priority patent/SG11201402341QA/en
Priority to JP2014542300A priority patent/JP2014533502A/en
Priority to AU2012337389A priority patent/AU2012337389A1/en
Priority to CA2855953A priority patent/CA2855953A1/en
Priority to US13/587,883 priority patent/US8703653B2/en
Priority to CN201280067273.7A priority patent/CN104093857A/en
Publication of WO2012112925A2 publication Critical patent/WO2012112925A2/en
Publication of WO2012112925A9 publication Critical patent/WO2012112925A9/en
Publication of WO2012112925A3 publication Critical patent/WO2012112925A3/en
Priority to IL227865A priority patent/IL227865A0/en
Priority to IL232586A priority patent/IL232586A0/en
Priority to HK15100214.3A priority patent/HK1199746A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Abstract

This invention provides methods of detecting and quantifying target nucleic acids in samples in multiplexed single chamber reactions. Consumables incorporating chambers optimized to reduce signal background proximal to high efficiency arrays are provided, as well as methods of use. Devices and systems configured to use the consumables to practice the methods are a feature of the invention.
PCT/US2012/025699 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids WO2012112925A2 (en)

Priority Applications (23)

Application Number Priority Date Filing Date Title
JP2013554647A JP2014511178A (en) 2011-02-18 2012-02-17 Quantitative and highly multiplexed detection of nucleic acids
EP12747077.1A EP2675920A4 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
SG2013060975A SG192720A1 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
CA2827040A CA2827040A1 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
MX2013009429A MX2013009429A (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids.
CN201280018955.9A CN103502471B (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
BR112013021144A BR112013021144A2 (en) 2011-02-18 2012-02-17 highly multiplexed quantitative detection of nucleic acids
AU2012219295A AU2012219295A1 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
KR1020137024679A KR20140044309A (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids
PCT/US2012/051236 WO2013074163A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
US13/587,883 US8703653B2 (en) 2011-02-18 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
EP12849697.3A EP2780479A4 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
BR112014011937A BR112014011937A2 (en) 2011-11-17 2012-08-16 quantitative highly multiplexed nucleic acid detection
KR1020147016411A KR20140094007A (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
SG11201402341QA SG11201402341QA (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
JP2014542300A JP2014533502A (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
AU2012337389A AU2012337389A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
CA2855953A CA2855953A1 (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
MX2014005912A MX338076B (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids.
CN201280067273.7A CN104093857A (en) 2011-11-17 2012-08-16 Quantitative, highly multiplexed detection of nucleic acids
IL227865A IL227865A0 (en) 2011-02-18 2013-08-08 Quantitative, highly multiplexed detection of nucleic acids
IL232586A IL232586A0 (en) 2011-11-17 2014-05-13 Quantitative, highly multiplexed detection of nucleic acids
HK15100214.3A HK1199746A1 (en) 2011-11-17 2015-01-08 Quantitative, highly multiplexed detection of nucleic acids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161463580P 2011-02-18 2011-02-18
US61/463,580 2011-02-18
US201161561198P 2011-11-17 2011-11-17
US61/561,198 2011-11-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/399,872 Continuation-In-Part US20120214686A1 (en) 2011-02-18 2012-02-17 Quantitative, Highly Multiplexed Detection of Nucleic Acids

Publications (3)

Publication Number Publication Date
WO2012112925A2 WO2012112925A2 (en) 2012-08-23
WO2012112925A9 WO2012112925A9 (en) 2013-03-14
WO2012112925A3 true WO2012112925A3 (en) 2013-05-02

Family

ID=46653242

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/025699 WO2012112925A2 (en) 2011-02-18 2012-02-17 Quantitative, highly multiplexed detection of nucleic acids

Country Status (12)

Country Link
US (1) US20120214686A1 (en)
EP (1) EP2675920A4 (en)
JP (1) JP2014511178A (en)
KR (1) KR20140044309A (en)
CN (1) CN103502471B (en)
AU (1) AU2012219295A1 (en)
BR (1) BR112013021144A2 (en)
CA (1) CA2827040A1 (en)
IL (1) IL227865A0 (en)
MX (1) MX2013009429A (en)
SG (1) SG192720A1 (en)
WO (1) WO2012112925A2 (en)

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KR20150135353A (en) 2013-03-14 2015-12-02 엔브이에스 테크놀로지스 인코포레이티드 Surface oxidation for sequestering biomolecules and related methods
BR112015023769A2 (en) * 2013-03-15 2017-07-18 Nvs Tech Inc analytical instrument systems
JP5871339B2 (en) * 2014-01-21 2016-03-01 株式会社ジェイ・パワーシステムズ Power cable
EP3283501B1 (en) 2015-04-16 2023-01-04 William Marsh Rice University Stoichiometric tuning of nucleic acid hybridization probes by auxiliary oligonucleotide species
CA2986151A1 (en) * 2015-05-20 2016-11-24 Quantum-Si Incorporated Pulsed laser and bioanalytic system
US10605730B2 (en) 2015-05-20 2020-03-31 Quantum-Si Incorporated Optical sources for fluorescent lifetime analysis
US11466316B2 (en) 2015-05-20 2022-10-11 Quantum-Si Incorporated Pulsed laser and bioanalytic system
JP6958547B2 (en) * 2016-04-27 2021-11-02 凸版印刷株式会社 Reaction vessel and biochemical analysis method
BR112019012069A2 (en) 2016-12-16 2019-11-12 Quantum-Si Incorporated compact beam targeting and modeling set
CA3047133A1 (en) 2016-12-16 2018-06-21 Quantum-Si Incorporated Compact mode-locked laser module
CN107247040A (en) * 2017-05-24 2017-10-13 重庆大学 Food-borne pathogens fluorescence detection device and method of work
CN108467889A (en) * 2018-04-04 2018-08-31 苏州创澜生物科技有限公司 A kind of fluorescence probe and its application for multiplex PCR
US11808700B2 (en) 2018-06-15 2023-11-07 Quantum-Si Incorporated Data acquisition control for advanced analytic instruments having pulsed optical sources
CN111277556B (en) * 2019-01-30 2023-04-07 维沃移动通信有限公司 Processing method and communication device
US11747561B2 (en) 2019-06-14 2023-09-05 Quantum-Si Incorporated Sliced grating coupler with increased beam alignment sensitivity
CN113122614B (en) * 2021-04-15 2021-11-16 珠海市尚维高科生物技术有限公司 Fluorescent quantitative PCR processing method and system

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Also Published As

Publication number Publication date
CN103502471B (en) 2015-04-22
BR112013021144A2 (en) 2016-11-22
SG192720A1 (en) 2013-09-30
EP2675920A4 (en) 2015-04-08
MX2013009429A (en) 2013-10-30
WO2012112925A9 (en) 2013-03-14
CN103502471A (en) 2014-01-08
US20120214686A1 (en) 2012-08-23
JP2014511178A (en) 2014-05-15
IL227865A0 (en) 2013-09-30
CA2827040A1 (en) 2012-08-23
WO2012112925A2 (en) 2012-08-23
AU2012219295A1 (en) 2013-10-10
EP2675920A2 (en) 2013-12-25
KR20140044309A (en) 2014-04-14

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