BR112014021993A2 - detecção da variação de nucleotídeo em sequência de ácido nucleico alvo através de ensaio de clivagem e extensão de pto - Google Patents

detecção da variação de nucleotídeo em sequência de ácido nucleico alvo através de ensaio de clivagem e extensão de pto

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Publication number
BR112014021993A2
BR112014021993A2 BR112014021993A BR112014021993A BR112014021993A2 BR 112014021993 A2 BR112014021993 A2 BR 112014021993A2 BR 112014021993 A BR112014021993 A BR 112014021993A BR 112014021993 A BR112014021993 A BR 112014021993A BR 112014021993 A2 BR112014021993 A2 BR 112014021993A2
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BR
Brazil
Prior art keywords
nucleic acid
acid sequence
target nucleic
nucleotide variation
extension assay
Prior art date
Application number
BR112014021993A
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English (en)
Other versions
BR112014021993B8 (pt
BR112014021993B1 (pt
Inventor
Yoon Chun Jong
Jo Lee Young
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of BR112014021993A2 publication Critical patent/BR112014021993A2/pt
Publication of BR112014021993B1 publication Critical patent/BR112014021993B1/pt
Publication of BR112014021993B8 publication Critical patent/BR112014021993B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

resumo patente de invenção: "detecção da variação de nucleotídeo em sequência de ácido nucleico alvo através de ensaio de clivagem e extensão de pto". a presente invenção refere-se em geral a um novo método e um kit para detecção de variações de nucleotídeo através de um ensaio de ptoce (clivagem e extensão de pto) com pto-nv. ainda, a presente invenção refere-se a um novo método e um kit para detecção de variação de nucleotídeo em uma sequência de ácido nucleico alvo através de um ensaio de ptoce com pto-nv tendo uma porção de não pareamento de base. 6/11 1/11
BR112014021993A 2012-03-05 2013-02-25 "métodos para detecção de uma variação de nucleotídeo em uma sequência de ácido nucleico alvo através de um ensaio de ptoce" BR112014021993B8 (pt)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261606713P 2012-03-05 2012-03-05
US61/606,713 2012-03-05
US201261613195P 2012-03-20 2012-03-20
US61/613,195 2012-03-20
US201261668628P 2012-07-06 2012-07-06
US61/668,628 2012-07-06
PCT/KR2013/001492 WO2013133561A1 (en) 2012-03-05 2013-02-25 Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay

Publications (3)

Publication Number Publication Date
BR112014021993A2 true BR112014021993A2 (pt) 2017-07-11
BR112014021993B1 BR112014021993B1 (pt) 2022-01-11
BR112014021993B8 BR112014021993B8 (pt) 2022-03-03

Family

ID=49116988

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014021993A BR112014021993B8 (pt) 2012-03-05 2013-02-25 "métodos para detecção de uma variação de nucleotídeo em uma sequência de ácido nucleico alvo através de um ensaio de ptoce"

Country Status (13)

Country Link
US (1) US9650665B2 (pt)
EP (1) EP2823061B1 (pt)
JP (1) JP5976849B2 (pt)
KR (1) KR101794981B1 (pt)
CN (1) CN104245959B (pt)
AU (1) AU2013228226B2 (pt)
BR (1) BR112014021993B8 (pt)
CA (1) CA2864523C (pt)
ES (1) ES2669244T3 (pt)
MX (1) MX354465B (pt)
RU (1) RU2620955C2 (pt)
WO (1) WO2013133561A1 (pt)
ZA (1) ZA201405894B (pt)

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WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
CN107109492B (zh) * 2014-12-22 2021-02-26 阿纳帕生物技术股份公司 用于靶核酸多重检测的双重猝灭测定
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
CN110273013B (zh) * 2018-03-13 2022-11-01 厦门大学 一种检测呼吸道病原体的方法
CN110273012B (zh) * 2018-03-13 2022-11-04 厦门大学 一种检测败血症病原体的方法
KR102220701B1 (ko) 2018-09-28 2021-03-03 (주)나노헬릭스 형광기반의 다중 융해 분석을 이용한 증폭 산물의 다중 분석방법

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Also Published As

Publication number Publication date
US20150167061A1 (en) 2015-06-18
JP5976849B2 (ja) 2016-08-24
CA2864523C (en) 2019-02-05
BR112014021993B8 (pt) 2022-03-03
CN104245959B (zh) 2017-10-13
MX2014010738A (es) 2015-06-17
CA2864523A1 (en) 2013-09-12
AU2013228226A1 (en) 2014-09-04
BR112014021993B1 (pt) 2022-01-11
RU2014138951A (ru) 2016-04-20
EP2823061B1 (en) 2018-02-14
CN104245959A (zh) 2014-12-24
AU2013228226B2 (en) 2016-06-02
RU2620955C2 (ru) 2017-05-30
EP2823061A4 (en) 2015-11-04
ZA201405894B (en) 2016-08-31
JP2015520603A (ja) 2015-07-23
EP2823061A1 (en) 2015-01-14
MX354465B (es) 2018-03-06
KR101794981B1 (ko) 2017-11-09
WO2013133561A1 (en) 2013-09-12
KR20140123561A (ko) 2014-10-22
US9650665B2 (en) 2017-05-16
ES2669244T3 (es) 2018-05-24

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B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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