BR112014010053A8 - variantes de isopreno sintase com solubilidade aprimorada para produção de isopreno - Google Patents

variantes de isopreno sintase com solubilidade aprimorada para produção de isopreno

Info

Publication number
BR112014010053A8
BR112014010053A8 BR112014010053A BR112014010053A BR112014010053A8 BR 112014010053 A8 BR112014010053 A8 BR 112014010053A8 BR 112014010053 A BR112014010053 A BR 112014010053A BR 112014010053 A BR112014010053 A BR 112014010053A BR 112014010053 A8 BR112014010053 A8 BR 112014010053A8
Authority
BR
Brazil
Prior art keywords
isoprene
production
present
enhanced solubility
isoprene synthase
Prior art date
Application number
BR112014010053A
Other languages
English (en)
Other versions
BR112014010053A2 (pt
Inventor
L Rife Christopher
H Wells Derek
Original Assignee
Danisco Us Inc
Goodyear Tire & Rubber
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc, Goodyear Tire & Rubber filed Critical Danisco Us Inc
Publication of BR112014010053A2 publication Critical patent/BR112014010053A2/pt
Publication of BR112014010053A8 publication Critical patent/BR112014010053A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03007—Pentalenene synthase (4.2.3.7)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L7/00—Compositions of natural rubber

Abstract

abstract the present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. in particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells. ------------------------------------------------------------------------------------------------------------ tradução do resumo resumo patente de invenção "variantes de isopreno sintase com solubilidade aprimorada para produção de isopreno". a presente invenção refere-se a métodos e composições de polipeptídeos variantes que têm atividade de isopreno sintase com solubilidade aprimorada. em particular, a presente invenção fornece iso-preno sintases variantes para a produção aumentada de isopreno em células hospedeiras recombinantes.
BR112014010053A 2011-10-27 2012-10-26 variantes de isopreno sintase com solubilidade aprimorada para produção de isopreno BR112014010053A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161552453P 2011-10-27 2011-10-27
PCT/US2012/062308 WO2013063528A2 (en) 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene

Publications (2)

Publication Number Publication Date
BR112014010053A2 BR112014010053A2 (pt) 2017-06-13
BR112014010053A8 true BR112014010053A8 (pt) 2017-06-20

Family

ID=47178953

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014010053A BR112014010053A8 (pt) 2011-10-27 2012-10-26 variantes de isopreno sintase com solubilidade aprimorada para produção de isopreno

Country Status (8)

Country Link
US (2) US8735134B2 (pt)
EP (1) EP2771460B1 (pt)
JP (1) JP6101275B2 (pt)
BR (1) BR112014010053A8 (pt)
CA (1) CA2853125A1 (pt)
DK (1) DK2771460T3 (pt)
HK (1) HK1201554A1 (pt)
WO (1) WO2013063528A2 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2737082A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
DK2771460T3 (en) 2011-10-27 2017-06-06 Danisco Us Inc ISOPRENE SYNTHASE VARIETIES WITH IMPROVED SOLUBILITY FOR THE PREPARATION OF ISOPRENE
WO2013096683A2 (en) * 2011-12-23 2013-06-27 Danisco Us Inc. Enhanced production of isoprene using marine bacterial cells
WO2014065271A1 (ja) * 2012-10-23 2014-05-01 積水化学工業株式会社 組換え細胞、並びに、イソプレンの生産方法
BR112016011006A2 (pt) 2013-11-22 2017-12-05 Ajinomoto Kk isopreno sintase modificada, polinucleotídeo, vetor de expressão, célula hospedeira, métodos para produzir uma isopreno sintase modificada, um monômero de isopreno modificado e um polímero de isopreno, polímero derivado de um monômero de isopreno, composição de borracha, e, pneu
CN105985971B (zh) * 2015-02-10 2021-01-22 中国科学院微生物研究所 异戊二烯合成酶基因及其应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8407828D0 (en) 1984-03-27 1984-05-02 Ontario Research Foundation In situ preparation
US4570029A (en) 1985-03-04 1986-02-11 Uop Inc. Process for separating isoprene
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
US5849970A (en) 1995-06-23 1998-12-15 The Regents Of The University Of Colorado Materials and methods for the bacterial production of isoprene
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
US20030033626A1 (en) 2000-07-31 2003-02-13 Hahn Frederick M. Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
US7371558B2 (en) 2002-10-04 2008-05-13 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
US20090076743A1 (en) 2007-08-01 2009-03-19 Drexel University Novel method for zero point detection
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
SG192545A1 (en) 2008-04-23 2013-08-30 Danisco Us Inc Isoprene synthase variants for improved microbial production of isoprene
EP2307552B1 (en) 2008-06-30 2022-10-26 The Goodyear Tire & Rubber Company Polymers of isoprene from renewable resources
ES2716231T3 (es) 2008-07-02 2019-06-11 Danisco Us Inc Procedimientos para producir isopreno
WO2010031076A2 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Conversion of prenyl derivatives to isoprene
CA2737082A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
EP2344629B1 (en) 2008-09-15 2018-01-24 Danisco US Inc. Systems using cell culture for production of isoprene
WO2010031062A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
ZA201102111B (en) 2008-09-15 2013-10-30 Danisco Us Inc Reduction of carbon dioxide emission during isoprene production by fermentation
WO2010078457A2 (en) 2008-12-30 2010-07-08 Danisco Us Inc. Methods of producing isoprene and a co-product
AU2010238770A1 (en) 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201120213A (en) 2009-06-17 2011-06-16 Danisco Us Inc Polymerization of isoprene from renewable resources
TWI427149B (zh) 2009-06-17 2014-02-21 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
EP2513021A2 (en) 2009-12-18 2012-10-24 Danisco US Inc. Purification of isoprene from renewable resources
JP5855576B2 (ja) 2009-12-22 2016-02-09 ダニスコ・ユーエス・インク イソプレンガスの生成量を増大させるための膜バイオリアクター
BR112012017345A2 (pt) 2009-12-23 2015-11-03 Danisco Us Inc "composições e métodos de pgl para a produção aumentada de isopreno e outros produtos com 6-fosfogliconolactonase (pgl)"
JP2014502148A (ja) * 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体
DK2771460T3 (en) 2011-10-27 2017-06-06 Danisco Us Inc ISOPRENE SYNTHASE VARIETIES WITH IMPROVED SOLUBILITY FOR THE PREPARATION OF ISOPRENE

Also Published As

Publication number Publication date
US20130295632A1 (en) 2013-11-07
WO2013063528A2 (en) 2013-05-02
EP2771460B1 (en) 2017-04-26
JP6101275B2 (ja) 2017-03-22
DK2771460T3 (en) 2017-06-06
JP2015503904A (ja) 2015-02-05
US9273299B2 (en) 2016-03-01
BR112014010053A2 (pt) 2017-06-13
US8735134B2 (en) 2014-05-27
EP2771460A2 (en) 2014-09-03
US20140315275A1 (en) 2014-10-23
WO2013063528A3 (en) 2013-06-20
CA2853125A1 (en) 2013-05-02
HK1201554A1 (en) 2015-09-04

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]