UA115082C2 - Спосіб детекції нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто - Google Patents

Спосіб детекції нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто

Info

Publication number
UA115082C2
UA115082C2 UAA201508100A UAA201508100A UA115082C2 UA 115082 C2 UA115082 C2 UA 115082C2 UA A201508100 A UAA201508100 A UA A201508100A UA A201508100 A UAA201508100 A UA A201508100A UA 115082 C2 UA115082 C2 UA 115082C2
Authority
UA
Ukraine
Prior art keywords
nucleic acid
detection
acid sequence
analysis
extension
Prior art date
Application number
UAA201508100A
Other languages
English (en)
Inventor
Йонг Йун Чан
Йоунг Йо Лі
Original Assignee
Сеегене, Інк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020110002840A external-priority patent/KR20120081482A/ko
Priority claimed from KR1020110023465A external-priority patent/KR20120105811A/ko
Application filed by Сеегене, Інк. filed Critical Сеегене, Інк.
Publication of UA115082C2 publication Critical patent/UA115082C2/uk

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

Abstract

Винахід стосується детекції нуклеїновокислотної послідовності-мішені в аналізі з РТОСЕ (розщепленням та подовженням РТО), при цьому РТО (олігонуклеотид, що зондує та мітить), гібридизований з нуклеїновокислотною послідовністю-мішенню, розщеплюється з вивільненням фрагмента, і цей фрагмент гібридизується з СТО (захоплюючим та матричним олігонуклеотидом) з утворенням подовженого дуплекса, після чого відбувається детекція присутності подовженого дуплекса. Подовжений дуплекс забезпечує одержання сигналів (генерацію, підсилення, гасіння або ослаблення сигналів) від міток, що вказують на присутність подовженого дуплекса, і має регульовану величину Тпл., яка є добре придатною для детекції присутності нуклеїновокислотної послідовності-мішені.
UAA201508100A 2011-01-11 2012-01-11 Спосіб детекції нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто UA115082C2 (uk)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020110002840A KR20120081482A (ko) 2011-01-11 2011-01-11 태그 프로브 절단 및 연장에 의한 타겟 핵산서열의 검출
KR1020110023465A KR20120105811A (ko) 2011-03-16 2011-03-16 Ｐｔｏ 절단 및 연장-의존적 시그널 발생에 의한 타겟 핵산서열의 검출
PCT/KR2011/004452 WO2012096430A1 (en) 2011-01-11 2011-06-17 Detection of target nucleic acid sequences by pto cleavage and extension assay
UAA201309884A UA110815C2 (uk) 2011-01-11 2012-11-01 Детекція нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто

Publications (1)

Publication Number Publication Date
UA115082C2 true UA115082C2 (uk) 2017-09-11

Family

ID=46507300

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201508100A UA115082C2 (uk) 2011-01-11 2012-01-11 Спосіб детекції нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто

Country Status (22)

Country Link
US (6) US8809239B2 (uk)
EP (6) EP2826868B1 (uk)
JP (3) JP5756854B2 (uk)
KR (1) KR101462192B1 (uk)
CN (2) CN103866037B (uk)
AU (1) AU2012205956B2 (uk)
BR (3) BR122019022348B1 (uk)
CA (3) CA2802494C (uk)
DK (1) DK2572004T3 (uk)
ES (3) ES2538459T3 (uk)
IL (2) IL223608A (uk)
IN (1) IN2013CN00284A (uk)
MX (3) MX2017015093A (uk)
MY (1) MY174052A (uk)
NO (1) NO343803B1 (uk)
PL (2) PL2708608T3 (uk)
PT (2) PT2572004E (uk)
RU (1) RU2566562C2 (uk)
SG (1) SG190758A1 (uk)
UA (1) UA115082C2 (uk)
WO (2) WO2012096430A1 (uk)
ZA (1) ZA201209536B (uk)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101903535A (zh) 2007-03-28 2010-12-01 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
MX2017015093A (es) 2011-01-11 2023-03-10 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
WO2012112925A2 (en) * 2011-02-18 2012-08-23 NVS Technologies, Inc. Quantitative, highly multiplexed detection of nucleic acids
WO2012134195A2 (en) 2011-03-29 2012-10-04 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
DE102011055247A1 (de) 2011-11-10 2013-05-16 Albert-Ludwigs-Universität Freiburg Multianalyt-Reportersystem
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
KR101794981B1 (ko) * 2012-03-05 2017-11-09 주식회사 씨젠 Ｐｔｏ 절단 및 연장 어세이에 의한 타겟 핵산서열에서의 뉴클레오타이드 변이 검출
EP2839031B1 (en) * 2012-04-19 2017-03-29 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage
CN105102630A (zh) * 2012-08-16 2015-11-25 Nvs技术股份有限公司 试验方法和系统
CA2896213C (en) * 2012-12-27 2018-12-11 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay
EP2770065B1 (en) * 2013-02-25 2017-12-13 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
WO2014142575A1 (en) * 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US10364459B2 (en) 2013-05-30 2019-07-30 Sugentech, Inc. Method of quantitatively and qualitatively analyzing biomaterial in real-time
EP3022319B1 (en) * 2013-07-15 2023-05-17 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
KR101778771B1 (ko) 2013-07-31 2017-09-18 주식회사 씨젠 Snp 유전자형을 결정하는 방법
CN105793435A (zh) 2013-10-09 2016-07-20 弗洛雷森特里克公司 多重探针
JP6295322B2 (ja) * 2013-10-18 2018-03-14 シージーン アイエヌシー ｈＣＴＯを利用するＰＴＯ切断及び延長分析による固相におけるターゲット核酸配列の検出
CN105452451B (zh) 2013-12-06 2020-06-05 生物辐射实验室股份有限公司 融合聚合酶
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
MX2017000391A (es) * 2014-07-10 2017-07-11 Fluoresentric Inc Tecnologia de amplificacion de adn.
DE102014110535A1 (de) 2014-07-25 2016-01-28 Certoplast Vorwerk & Sohn Gmbh Verfahren zur Herstellung eines Klebebandes
CN106715720B (zh) 2014-08-11 2021-08-13 卢米耐克斯公司 用于在核酸测定中改善解链分辨和多重性的探针
KR101750464B1 (ko) 2014-11-28 2017-06-28 케이맥바이오센터주식회사 바이오물질의 실시간 정량 및 정성 분석을 위한 프로브 시스템, 상기 프로브 시스템을 구비한 반응용기 및 이를 이용한 분석 방법
FR3029290B1 (fr) * 2014-12-01 2020-03-06 Biomerieux Procede d'estimation de l'affinite sonde-cible d'une puce a adn et procede de fabrication d'une puce a adn
KR102019506B1 (ko) 2014-12-09 2019-09-19 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
US20170362646A1 (en) * 2014-12-09 2017-12-21 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures and reference values
WO2017046192A1 (de) * 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre nukleinsäure-amplifikate in einem kontinuierlichen reaktionsansatz und unmittelbare auswertung mittels elektrophoretischer verfahren
DE102015115836A1 (de) * 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre Nukleinsäure-Amplifikate in einem kontinuierlichen Reaktionsansatz und unmittelbare Auswertung mittels immunchromatographischer Verfahren
EP3378000B1 (en) 2015-11-20 2021-09-01 Seegene, Inc. Method for calibrating a data set of a target analyte
CN108368547B (zh) 2015-12-15 2022-04-05 Seegene株式会社 与靶核酸序列有关的信号提取
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
KR102300738B1 (ko) 2016-02-05 2021-09-10 주식회사 씨젠 타겟 분석물질에 대한 데이터 세트의 노이즈 수준 감축 방법
WO2017213458A1 (en) 2016-06-10 2017-12-14 Seegene, Inc. Methods for preparing tagging oligonucleotides
US11602752B2 (en) 2016-06-30 2023-03-14 Seegene, Inc. Apparatus for amplificating nucleic acid and fluorescence-detecting device
CN109689889A (zh) 2016-09-15 2019-04-26 豪夫迈·罗氏有限公司 用于进行多重pcr的方法
EP3523452A4 (en) 2016-10-06 2020-06-10 Seegene, Inc. METHOD FOR PRODUCING OLIGONUCLEOTIDES FOR DETECTING TARGET NUCLEIC ACID MOLECULES IN SAMPLES
BR112019012925A2 (pt) 2016-12-23 2019-12-10 Albert-Ludwigs-Universität Freiburg sonda mediadora em duas partes
EP3562958B1 (en) 2016-12-29 2021-11-03 Seegene, Inc. Method for reducing primer dimer formation and increasing amplification efficiency
RU2665631C2 (ru) 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックＩｐマネジメント株式会社 核酸検出用オリゴヌクレオチド
CN108823287B (zh) 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
DE102017210140A1 (de) 2017-06-16 2018-12-20 Fidlock Gmbh Verschlussvorrichtung zum lösbaren Verbinden zweier Teile
CN107236815A (zh) * 2017-07-18 2017-10-10 江西贤聚景欣医药生物科技有限公司 用于靶核酸序列检测的多重淬灭荧光探针及方法
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
EP3688188A4 (en) 2017-09-29 2021-06-16 Seegene, Inc. DETECTION OF TARGET NUCLEAR ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION DEPENDENT NON-HYBRIDIZATION TEST
KR102369388B1 (ko) 2018-01-05 2022-03-02 주식회사 씨젠 시료에서 M. tuberculosis, M. bovis 및 M. bovis BCG의 존재 또는 부존재를 결정하는 방법
BR112020014412A2 (pt) 2018-01-22 2020-12-29 Luminex Corporation Métodos e composições para análise de fusão discreta
KR102464501B1 (ko) 2018-04-20 2022-11-17 주식회사 씨젠 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치
WO2020127620A1 (en) 2018-12-20 2020-06-25 F. Hoffmann-La Roche Ag Detection of target nucleic acid by solid-phase molography
EP4163397A1 (en) 2020-06-05 2023-04-12 Seegene, Inc. Specimen transport kit for detecting respiratory pathogens and methods for detecting respiratory pathogens using same
US20230295739A1 (en) * 2020-11-23 2023-09-21 Pleno, Inc. Encoded Endonuclease Assays
US20230068047A1 (en) * 2021-08-19 2023-03-02 Luminex Corporation Digital amplification assay analysis method
WO2023043280A1 (ko) * 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
WO2023075394A1 (ko) 2021-10-29 2023-05-04 주식회사 씨젠 검체 채취 스왑 도구 및 호흡기 병원체의 검출 방법
WO2023108014A1 (en) * 2021-12-07 2023-06-15 Luminex Corporation Methods and compositions for nucleic acid analysis
CN114032337A (zh) * 2021-12-14 2022-02-11 广州市金圻睿生物科技有限责任公司 一种呼吸道病原体检测试剂盒及其制备方法与应用
CN114134219A (zh) * 2021-12-14 2022-03-04 广州市金圻睿生物科技有限责任公司 一种多重核酸检测系统及其制备方法与应用
WO2023203230A1 (de) 2022-04-22 2023-10-26 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4265734A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5965364A (en) 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
EP0730662A4 (en) 1993-09-10 1999-11-24 Genevue Inc OPTICAL DETECTION OF THE POSITION OF OLIGONUCLEOTIDES ON LARGE DNA MOLECULES
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2257109C (en) 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
EP0963997B1 (en) 1996-11-18 2003-02-19 Takeshi Imanishi Novel nucleotide analogues
JP4362150B2 (ja) 1996-11-29 2009-11-11 サード ウェーブ テクノロジーズ，インコーポレーテッド Ｆｅｎ−１エンドヌクレアーゼ、混合物、および開裂方法
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
JP4236812B2 (ja) 1997-09-12 2009-03-11 エクシコン エ／エス オリゴヌクレオチド類似体
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6322980B1 (en) 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence
JP4724380B2 (ja) 1999-04-20 2011-07-13 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
WO2000079009A2 (en) 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US6265170B1 (en) * 2000-01-24 2001-07-24 Ingeneus Corporation Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
US7348141B2 (en) 2000-03-29 2008-03-25 Lgc Limited Hybridization beacon and method of rapid sequence detection and discrimination
ATE497018T1 (de) 2000-05-19 2011-02-15 Eragen Biosciences Inc Materialien und methoden zum nachweis von nukleinsäuren
US7309573B2 (en) * 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7678541B2 (en) 2000-11-21 2010-03-16 Hologic, Inc. Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
US20030165865A1 (en) * 2001-01-29 2003-09-04 Hinkel Christopher A. Methods of analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
WO2003033741A1 (en) 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
EP1481090A4 (en) 2002-02-15 2006-08-09 Somalogic Inc METHOD AND REAGENTS FOR DETECTING BINDING OF TARGET MOLECULES BY NUCLEIC ACID ELECTRODES
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20080187910A1 (en) * 2002-06-26 2008-08-07 Nilsen Thor W Nucleic acid assay and method of detecting the presence of a nucleic acid sequence
KR100557474B1 (ko) 2002-09-26 2006-03-07 요시다 켄지 도트 패턴을 이용한 정보재생, 입출력방법, 정보재생장치,휴대정보 입출력 장치 및 전자완구
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
AU2003221408B2 (en) 2003-03-17 2010-05-27 Kenji Yoshida Information input/output method using dot pattern
WO2005010199A2 (en) 2003-07-16 2005-02-03 Geneohm Sciences, Inc. Invasive cleavage reaction with electrochemical readout
US20050142595A1 (en) 2003-11-07 2005-06-30 U.S. Genomics, Inc. Intercalator FRET donors or acceptors
EP1698897B1 (en) 2003-12-19 2010-03-17 Nippon Steel Kankyo Engineering Co., Ltd. Novel mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor
US20050191682A1 (en) 2004-02-17 2005-09-01 Affymetrix, Inc. Methods for fragmenting DNA
WO2006005081A2 (en) 2004-06-30 2006-01-12 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
WO2006004949A1 (en) * 2004-06-30 2006-01-12 Applera Corporation Compositions and methods for detecting and quantitating polynucleotide sequences
RU2400538C2 (ru) 2005-03-05 2010-09-27 Сиджен, Инк. Олигонуклеотид с двойственной специфичностью и способы, в которых он используется
WO2006128010A2 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
US20070099211A1 (en) 2005-07-15 2007-05-03 Vissarion Aivazachvili Detection of nucleic acid amplification
EP2118310B1 (en) 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
EP2069525A1 (en) 2006-12-29 2009-06-17 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
FI20075124A0 (fi) 2007-02-21 2007-02-21 Valtion Teknillinen Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
KR20090067334A (ko) 2007-12-21 2009-06-25 주식회사 씨젠 핵산 중합효소의 뉴클레아제 활성을 이용한 타깃핵산분자의 검출방법
MX2010010161A (es) * 2008-03-15 2011-03-21 Hologic Inc Star Composiciones y metodos para el analisis de moleculas de acido nucleico durante reacciones de amplificacion.
US20110136118A1 (en) * 2008-06-17 2011-06-09 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
CN104673888B (zh) 2008-08-15 2017-09-19 卡斯凯德生物体系股份有限公司 检测核酸
WO2010030716A1 (en) * 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
WO2010055134A1 (en) 2008-11-13 2010-05-20 Riboxx Gmbh Rna detection method
EP2256215A1 (en) 2009-04-30 2010-12-01 Steffen Mergemeier Assay system using a nuclease activity of a nucleic acid polymerase
EP2248915B1 (en) 2009-05-05 2013-09-18 Qiagen GmbH Detection of multiple nucleic acid sequences in a reaction cartridge
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
EP2516679B1 (en) 2009-12-21 2017-08-23 Seegene, Inc. Tsg primer target detection
US20110281266A1 (en) 2010-05-14 2011-11-17 Life Technologies Corporation Identification of Nucleic Acids
US9921165B2 (en) * 2010-11-05 2018-03-20 Genalyte, Inc. Optical analyte detection systems and methods of use
MX2017015093A (es) 2011-01-11 2023-03-10 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
WO2012134195A2 (en) 2011-03-29 2012-10-04 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출

Also Published As

Publication number Publication date
EP2572004A2 (en) 2013-03-27
IN2013CN00284A (uk) 2015-10-02
PT2708608T (pt) 2017-06-26
RU2012142160A (ru) 2015-02-20
EP3543352A1 (en) 2019-09-25
AU2012205956B2 (en) 2015-07-16
WO2012096430A1 (en) 2012-07-19
IL223608A (en) 2016-08-31
AU2012205956A1 (en) 2013-01-31
NO20121065A1 (no) 2012-12-18
US10280453B2 (en) 2019-05-07
SG190758A1 (en) 2013-08-30
CA2802494C (en) 2019-06-18
CA2979858C (en) 2019-06-18
BR112012026221A2 (pt) 2017-01-10
CA3023333C (en) 2020-06-16
BR122019022348B1 (pt) 2021-05-11
PL2708608T3 (pl) 2017-09-29
ES2628327T3 (es) 2017-08-02
US20150086984A1 (en) 2015-03-26
KR20130006477A (ko) 2013-01-16
WO2012096523A3 (en) 2012-12-06
BR112012026221B1 (pt) 2021-03-02
WO2012096523A2 (en) 2012-07-19
US20200087718A1 (en) 2020-03-19
US10519489B2 (en) 2019-12-31
NZ604338A (en) 2015-05-29
US20130109588A1 (en) 2013-05-02
EP3168311B1 (en) 2019-05-15
EP3543352B1 (en) 2021-10-20
JP2017070298A (ja) 2017-04-13
EP2708608B1 (en) 2017-03-15
EP2572004A4 (en) 2013-05-29
NO343803B1 (no) 2019-06-11
EP2572004B1 (en) 2015-03-04
IL243623A (en) 2017-06-29
WO2012096523A9 (en) 2014-01-23
JP2015070848A (ja) 2015-04-16
EP3967774A1 (en) 2022-03-16
US20160312271A1 (en) 2016-10-27
US20170073744A1 (en) 2017-03-16
EP2826868A1 (en) 2015-01-21
MX2013001510A (es) 2013-02-27
ZA201209536B (en) 2014-03-26
CN102959092A (zh) 2013-03-06
RU2566562C2 (ru) 2015-10-27
ES2538459T3 (es) 2015-06-22
US20220213535A1 (en) 2022-07-07
US9540681B2 (en) 2017-01-10
MX352460B (es) 2017-11-24
JP6054357B2 (ja) 2016-12-27
CA2979858A1 (en) 2012-07-19
MX340258B (es) 2016-07-01
MY174052A (en) 2020-03-05
NZ706144A (en) 2016-11-25
PT2572004E (pt) 2015-07-07
MX2017015093A (es) 2023-03-10
DK2572004T3 (da) 2015-06-08
BR122019022355B1 (pt) 2021-05-11
EP2826868B1 (en) 2017-03-08
KR101462192B1 (ko) 2014-11-14
CN103866037B (zh) 2016-09-07
CN103866037A (zh) 2014-06-18
CN102959092B (zh) 2016-01-13
JP6244440B2 (ja) 2017-12-06
EP2708608A1 (en) 2014-03-19
CA2802494A1 (en) 2012-07-19
CA3023333A1 (en) 2012-07-19
ES2629759T3 (es) 2017-08-14
JP2013538041A (ja) 2013-10-10
US8809239B2 (en) 2014-08-19
PL2572004T3 (pl) 2015-08-31
US11306349B2 (en) 2022-04-19
EP3168311A1 (en) 2017-05-17
JP5756854B2 (ja) 2015-07-29

Similar Documents

Publication Publication Date Title
UA115082C2 (uk) Спосіб детекції нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто
EP4299760A3 (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2012134195A3 (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent cleavage
NZ701145A (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
MX354465B (es) Deteccion de variacion del nucleotido en la secuencia de acido nucleico de objetivo por ensayo de desdoblamiento y extension de pto.
GB201317477D0 (en) Methods, compositions and kits for determing the presence/absnece of a variant nucleic acid sequence
EP2504453A4 (en) ASSAY METHOD FOR TARGET NUCLEIC ACID BY SIGNAL AMPLIFICATION USING HYBRIDIZATION AND RESTRICTION OF PROBES
IN2014DN04645A (uk)
IN2015DN00194A (uk)
PE20120616A1 (es) Sondas y cebadores para la deteccion de chikungunya
CL2022000131A1 (es) Método para detectar la presencia de un gen restaurador rf3 para el gen de la esterilidad masculina citoplasmática de tipo s del maíz. (divisional de solicitud 201601658)
CY1116130T1 (el) Μεθοδοι δοκιμασιας για mdv-1
BR112012030813A2 (pt) "métodos e kits para detecção in situ de sequências de nucleotídeo"
WO2012054933A3 (en) Universal probe assay methods
PH12016502411A1 (en) Method for methylation analysis
BR112015000911A2 (pt) iniciadores cooperativos, sondas e suas respectivas aplicações
UA110815C2 (uk) Детекція нуклеїновокислотної послідовності-мішені в аналізі із розщепленням та подовженням рто
DE602008006254D1 (de) Doppel-oligonukleotid-nukleinsäurenachweisverfahren
BR122020012978A8 (pt) Método de quantificação de um produto específico em uma reação de amplificação por corte e extensão (near)
MX2017006898A (es) Amplificacion y deteccion de acidos nucleicos en una muestra biologica.
WO2019066461A3 (en) Detection of target nucleic acid sequences by pto cleavage and extension-dependent extension assay
EP3022319A4 (en) Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
CA2829852C (en) Dual probe assay for the detection of hcv
CL2012000569A1 (es) Metodo para cuantificar acidos nucleicos que comprende etiquetar un oligonucleotido de referencia, amplificar un acido nucleico objetivo, comparar la intensidad del acido nucleico objetivo con el de referencia para determinar su cantidad.
MX2015001135A (es) Analisis de cigosis de criterio de valoracion para detectar el gen rf4 en el maiz.