PE20120616A1 - Sondas y cebadores para la deteccion de chikungunya - Google Patents
Sondas y cebadores para la deteccion de chikungunyaInfo
- Publication number
- PE20120616A1 PE20120616A1 PE2011001546A PE2011001546A PE20120616A1 PE 20120616 A1 PE20120616 A1 PE 20120616A1 PE 2011001546 A PE2011001546 A PE 2011001546A PE 2011001546 A PE2011001546 A PE 2011001546A PE 20120616 A1 PE20120616 A1 PE 20120616A1
- Authority
- PE
- Peru
- Prior art keywords
- seq
- chikungunya
- primers
- infection
- probes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/101—Taqman
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
SE REFIERE A UN METODO PARA DETECTAR Y CUANTIFICAR LA INFECCION POR CHIKUNGUNYA, EL CUAL COMPRENDE: A) FORMAR UNA MEZCLA DE REACCION QUE COMPRENDE i) UNA MUESTRA TAL COMO SANGRE, SUERO O PLASMA QUE VA A DETECTARSE, ii) REACTIVOS DE AMPLIFICACION DE ACIDO NUCLEICO, iii) SONDAS QUE COMPRENDEN LA SEQ ID Nº 1 Y 2 CONJUGADAS CON MARCADORES DETECTABLES TALES COMO UN FLUOROFORO EN EL EXTREMO 5' QUE ES 6-CARBOXIFLUORESCEINA Y UN EXTINTOR EN EL EXTREMO 3' QUE ES TETRAMETILRODAMINA, Y iv) CEBADORES QUE COMPRENDEN LA SEQ ID Nº 3, 4, 5 Y 6 CORRESPONDIENDO LOS CEBADORES QUE TIENEN SEQ ID Nº 3 Y 5 A LA SONDA DE SEQ ID Nº 1 Y CORRESPONDIENDO LOS CEBADORES QUE TIENEN SEQ ID Nº 4 Y 6 A LA SONDA DE SEQ ID Nº 2; B) SOMETER LA MEZCLA DE REACCION A PCR PARA OBTENER COPIAS DE LA SECUENCIA DIANA SEGUIDO DE LA MEDICION DE LA SENAL DE FLUORESCENCIA PARA DETECTAR LA INFECCION POR CHIKUNGUNYA; Y C) OPCIONALMENTE CONSTRUIR UNA CURVA PATRON A PARTIR DE LA SENAL DETECTADA PARA OBTENER EL NUMERO DE COPIAS PARA CUANTIFICAR LA INFECCION POR CHIKUNGUNYA
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN439CH2009 | 2009-02-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20120616A1 true PE20120616A1 (es) | 2012-05-26 |
Family
ID=42665059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2011001546A PE20120616A1 (es) | 2009-02-25 | 2010-02-23 | Sondas y cebadores para la deteccion de chikungunya |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20120045761A1 (es) |
| EP (1) | EP2401286A4 (es) |
| JP (1) | JP2012518431A (es) |
| KR (1) | KR20110118176A (es) |
| CN (1) | CN102414217A (es) |
| AU (1) | AU2010217230B2 (es) |
| CO (1) | CO6430470A2 (es) |
| EA (1) | EA201171046A1 (es) |
| MX (1) | MX2011008970A (es) |
| PE (1) | PE20120616A1 (es) |
| SG (1) | SG173822A1 (es) |
| WO (1) | WO2010097806A1 (es) |
| ZA (1) | ZA201106432B (es) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935715B (zh) * | 2010-09-08 | 2012-02-22 | 中国检验检疫科学研究院 | 实时荧光定量pcr检测基孔肯亚病毒核酸的方法 |
| JP6205354B2 (ja) | 2011-07-06 | 2017-09-27 | ギリアード サイエンシーズ， インコーポレイテッド | Ｈｉｖの処置のための化合物 |
| CN102268489A (zh) * | 2011-08-23 | 2011-12-07 | 中国检验检疫科学研究院 | 纳米金荧光定量pcr检测基孔肯雅病毒核酸的非诊断性方法 |
| CN102443051B (zh) * | 2011-09-30 | 2017-07-18 | 中国人民解放军广州军区疾病预防控制中心 | 一种检测基孔肯雅病毒的重组抗原蛋白、试剂盒及其应用 |
| WO2013171485A1 (en) * | 2012-05-18 | 2013-11-21 | Medical Research Council | Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase. |
| TWI706945B (zh) | 2013-03-01 | 2020-10-11 | 美商基利科學股份有限公司 | 供治療反轉錄病毒科病毒感染之治療性化合物 |
| US10202353B2 (en) | 2014-02-28 | 2019-02-12 | Gilead Sciences, Inc. | Therapeutic compounds |
| CN104513865B (zh) * | 2014-11-25 | 2016-02-24 | 扬州大学 | 反转录pcr检测基孔肯雅病毒的试剂盒及其检测方法 |
| CN104745692A (zh) * | 2015-03-10 | 2015-07-01 | 普生（天津）科技有限公司 | 基孔肯亚病毒序列 |
| EP4265299A3 (en) | 2016-08-19 | 2024-01-17 | Gilead Sciences, Inc. | Therapeutic compounds useful for the prophylactic or therapeutic treatment of an hiv virus infection |
| US20190330706A1 (en) * | 2016-08-26 | 2019-10-31 | The Broad Institute, Inc. | Nucleic acid amplification assays for detection of pathogens |
| TW202024061A (zh) | 2017-08-17 | 2020-07-01 | 美商基利科學股份有限公司 | Hiv蛋白質膜抑制劑之固體形式 |
| AR112412A1 (es) | 2017-08-17 | 2019-10-23 | Gilead Sciences Inc | Formas de sal de colina de un inhibidor de la cápside del vih |
| KR102030245B1 (ko) * | 2017-10-12 | 2019-10-08 | 고려대학교 산학협력단 | 치쿤군야 바이러스 검출용 올리고뉴클레오티드 세트 및 이의 용도 |
| KR102587510B1 (ko) | 2018-02-15 | 2023-10-11 | 길리애드 사이언시즈, 인코포레이티드 | 피리딘 유도체 및 hiv 감염을 치료하기 위한 그의 용도 |
| JP7038843B2 (ja) | 2018-02-16 | 2022-03-18 | ギリアード サイエンシーズ， インコーポレイテッド | Ｒｅｔｒｏｖｉｒｉｄａｅウイルス感染の処置において有用な治療用化合物を調製するための方法および中間体 |
| KR20230141905A (ko) | 2018-07-16 | 2023-10-10 | 길리애드 사이언시즈, 인코포레이티드 | Hiv의 치료를 위한 캡시드 억제제 |
| US11807625B2 (en) | 2019-11-26 | 2023-11-07 | Gilead Sciences, Inc. | Capsid inhibitors for the prevention of HIV |
| CA3181690A1 (en) | 2020-06-25 | 2021-12-30 | Chienhung CHOU | Capsid inhibitors for the treatment of hiv |
| CA3235937A1 (en) | 2021-12-03 | 2023-06-08 | Gilead Sciences, Inc. | Therapeutic compounds for hiv virus infection |
| KR20230111359A (ko) | 2022-01-18 | 2023-07-25 | 한국표준과학연구원 | 전장유전체 증폭을 위한 치쿤군야 바이러스 범용 프라이머 세트 및 이를 이용한 진단 키트 |
| KR20230111360A (ko) | 2022-01-18 | 2023-07-25 | 한국표준과학연구원 | 치쿤군야 바이러스 검출용 프라이머 세트 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2545597A1 (en) * | 2006-03-15 | 2007-09-15 | Institut Pasteur | Novel isolated and purified strains of chikungunya virus and polynucleotides and polypeptides sequences, diagnostic and immunogenical uses thereof |
| FR2921064A1 (fr) * | 2007-09-14 | 2009-03-20 | Biomerieux Sa | Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence du gene e1 du virus du chikungunya |
| CN101270394B (zh) * | 2008-05-05 | 2011-08-03 | 广东出入境检验检疫局检验检疫技术中心 | 一种用于基孔肯雅病毒检测的引物 |
-
2010
- 2010-02-23 US US13/203,286 patent/US20120045761A1/en not_active Abandoned
- 2010-02-23 EA EA201171046A patent/EA201171046A1/ru unknown
- 2010-02-23 MX MX2011008970A patent/MX2011008970A/es not_active Application Discontinuation
- 2010-02-23 JP JP2011551569A patent/JP2012518431A/ja active Pending
- 2010-02-23 PE PE2011001546A patent/PE20120616A1/es not_active Application Discontinuation
- 2010-02-23 KR KR1020117022190A patent/KR20110118176A/ko not_active Application Discontinuation
- 2010-02-23 SG SG2011060787A patent/SG173822A1/en unknown
- 2010-02-23 EP EP10745891A patent/EP2401286A4/en not_active Withdrawn
- 2010-02-23 AU AU2010217230A patent/AU2010217230B2/en not_active Ceased
- 2010-02-23 WO PCT/IN2010/000103 patent/WO2010097806A1/en active Application Filing
- 2010-02-23 CN CN2010800183040A patent/CN102414217A/zh active Pending
-
2011
- 2011-08-31 ZA ZA2011/06432A patent/ZA201106432B/en unknown
- 2011-09-22 CO CO11123963A patent/CO6430470A2/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| MX2011008970A (es) | 2012-03-06 |
| EA201171046A1 (ru) | 2012-04-30 |
| AU2010217230B2 (en) | 2012-05-10 |
| AU2010217230A1 (en) | 2011-09-22 |
| EP2401286A1 (en) | 2012-01-04 |
| US20120045761A1 (en) | 2012-02-23 |
| ZA201106432B (en) | 2012-04-25 |
| WO2010097806A1 (en) | 2010-09-02 |
| CN102414217A (zh) | 2012-04-11 |
| CO6430470A2 (es) | 2012-04-30 |
| SG173822A1 (en) | 2011-09-29 |
| EP2401286A4 (en) | 2012-09-26 |
| JP2012518431A (ja) | 2012-08-16 |
| KR20110118176A (ko) | 2011-10-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |